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| 1 | Bcl-x_L and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells显示文摘AIM: To explore the role of Bcl-xL and Myeloid cell leukaemia (Mcl)-1 for the apoptosis resistance of colorectal carcinoma (CRC) cells towards current treat-ment modalities. METHODS: Bcl-xL and Mcl-1 mRNA and protein ex-pression were analyzed in CRC cell lines as well as human CRC tissue by Western blot,quantitative PCRand immunohistochemistry. Bcl-xL and Mcl-1 protein expression was knocked down or increased in CRC cell lines by applying specific siRNAs or expression plas-mids,respectively. After modulation of protein expres-sion,CRC cells were treated with chemotherapeutic agents,an antagonistic epidermal growth factor recep-tor (EGFR1) antibody,an EGFR1 tyrosine kinase inhibi-tor,or with the death receptor ligand TRAIL. Apoptosis induction and cell viability were analyzed. RESULTS: Here we show that in human CRC tis-sue and various CRC cell lines both Bcl-xL and Mcl-1 are expressed. Bcl-xL expression was higher in CRC tissue than in surrounding non-malignant tissue,both on protein and mRNA level. Mcl-1 mRNA expression was significantly lower in ma-lignant tissues. However,protein expression was slightly higher. Viability rates of CRC cells were significantly decreased after knock down of Bcl-xL expression,and,to a lower extent,after knock down of Mcl-1 expression. Furthermore,cells with reduced Bcl-xL or Mcl-1 expression was more sensitive towards oxaliplatin-and irinotecan-induced apoptosis,and in the case of Bcl-xL also towards 5-FU-induced apoptosis. On the other hand,upregulation of Bcl-xL by transfec-tion of an expression plasmid decreased chemothera-peutic drug-induced apoptosis. EGF treatment clearly induced Bcl-xL and Mcl-1 expression in CRC cells. Apop-tosis induction upon EGFR1 blockage by cetuximab or PD168393 was increased by inhibiting Mcl-1 and Bcl-xL expression. More strikingly,CD95-and TRAIL-induced apoptosis was increased by Bcl-xL knock down. CONCLUSION: Our data suggest that Bcl-xL and,to a lower extent,Mcl-1,are important anti-apoptotic factors in CRC. Specific downregulation of Bcl-xL is a promising approach to sensitize CRC cells towards chemotherapy and targeted therapy. | Henning Schulze-Bergkamen Roland Ehrenberg Lothar Hickmann Binje Vick Toni Urbanik Christoph C Schimanski Martin R Berger Arno Schad Achim Weber Steffen Heeger Peter R Galle Markus Moehler | 2008 | World Journal of Gastroenterology2008，14，24： | 4 |
| 2 | 22-gauge core vs 22-gauge aspiration needle for endoscopic ultrasound-guided sampling of abdominal masses显示文摘AIM To compare the aspiration needle(AN) and core biopsy needle(PC) in endoscopic ultrasound-guided fine needle aspiration(EUS-FNA) of abdominal masses.METHODS Consecutive patients referred for EUS-FNA were included in this prospective single-center trial. Each patient underwent a puncture of the lesion with both standard 22-gauge(G) AN(Echo Tip Ultra; Cook Medical, Bloomington, Indiana, United States) and the novel 22 G PC(Echo Tip Pro Core; Cook Medical, Bloomington, Indiana, United States) in a randomized fashion; histology was attempted in the PC group only. The main study endpoint was the overall diagnostic accuracy, including the contribution of histology to the final diagnosis. Secondary outcome measures included material adequacy, number of needle passes, and complications.RESULTS Fifty six consecutive patients(29 men; mean age 68 years) with pancreatic lesions(n = 38), lymphadenopathy(n = 13), submucosal tumors(n = 4), or others lesions(n = 1) underwent EUS-FNA using both of the needles in a randomized order. AN and PC reached similar overall results for diagnostic accuracy(AN: 88.9 vs PC: 96.1, P = 0.25), specimen adequacy(AN: 96.4% vs PC: 91.1%, P = 0.38), mean number of passes(AN: 1.5 vs PC: 1.7, P = 0.14), mean cellularity score(AN: 1.7 vs PC: 1.1, P = 0.058), and complications(none). A diagnosis on the basis of histology was achieved in the PC group in 36(64.3%) patients, and in 2 of those as the sole modality. In patients with available histology the mean cellularity score was higher for AN(AN: 1.7 vs PC: 1.0, P = 0.034); no other differences were of statistical significance.CONCLUSION Both needles achieved high overall diagnostic yields and similar performance characteristics for cytological diagnosis; histological analysis was only possible in 2/3 of cases with the new needle. | William Sterlacci Athanasios D Sioulas Lothar Veits Pervin G?nüllü Guido Schachschal Stefan Groth Mario Anders Christos K Kontos Theodoros Topalidis Andrea Hinsch Michael Vieth Thomas R?sch Ulrike W Denzer | 2016 | World Journal of Gastroenterology2016，22，39： | 2 |
| 3 | The role of automated urine particle flow cytometry in clinical practice显示文摘 | Joris R Delanghe Timo T Kouri Andreas R Huber Kurt Hannemann-Pohl Walter G Guder Andreas Lun Pranav Sinha Gudrun Stamminger Lothar Beier | 2000 | Clinica Chimica Acta2000，，1： | 1 |
| 4 | Matched Field Inversion of Broadband Data Using the Freeze Bath Method显示文摘 | Lothar Jaschke Chapman N R | 1999 | JA SA1999，1064，： | 1 |
| 5 | Square regularization matrices for large linear discrete ill-posed problems 显示文摘 | Marco D Arthur N Lothar R | 2012 | Numerical Linear Algebra with Applications2012，19，6： | 1 |
| 6 | Reduced Number of Circulating Endothelial Progenitor Cells Predicts Future Cardiovascular Events: Proof of Concept for the Clinical Importance of Endogenous Vascular Repair显示文摘 | Caroline SL Lothar R Stephan F | 2005 | Circulation2005，111，22： | 1 |
| 7 | Recent progress in the tumor necrosis factor-α field显示文摘 | Lothar R Holter K | 1996 | Int Arch Allergy Immunol1996，111，： | 1 |
| 8 | Neural networks for on -line prediction and optimization of circulating fluidized bed process steps显示文摘 | LOTHAR R | 2000 | Powder Technology2000，111，1： | 1 |
| 9 | Matched field inversion of broadband data using the freeze bath method 显示文摘 | Lothar Jaschke Chapman N R | 1999 | JASA1999，1064，： | 1 |
| 10 | Reduced number of circulating endothelial progeni-tor cells predicts future cardiovascular events proof of concept for the clinical importance of endogenous vascular repair显示文摘 | CAROLINE S L LOTHAR R FICHTLSCHERER S | 2005 | Circulation2005，111，： | 1 |
| 11 | Zinc Homeostasis and Immunity显示文摘 | LOTHAR R HAJO H | 2007 | Trends in Immunology2007，28，1： | 1 |
| 12 | Reduced number ofcirculating endothelial progenitor cells predicts future cardiovascu-lar events proof of concept for the clinical importance of endoge-nous vascular repair显示文摘 | Caroline SL Lothar R Fichtlscherer S al | 2005 | Circulation2005，111，22： | 1 |
| 13 | Early electroencephalography in acute ischemic stroke: Prediction of a malignant course?显示文摘 | Lothar Burghaus Rüdiger Hilker Christian Dohmen Bert Bosche Lutz Winhuisen Norbert Galldiks Brigitte Szelies Wolf-Dieter Heiss | 2006 | Clinical Neurology and Neurosurgery2006，，1： | 1 |
| 14 | The thermopile: the commercial infrared detector显示文摘 | | 1988 | EOSD Sept1988，3，： | 1 |
| 15 | Early electroencephalography in acute ischemic stroke: Prediction of a malignant course?显示文摘 | Lothar Burghaus Rüdiger Hilker Christian Dohmen Bert Bosche Lutz Winhuisen Norbert Galldiks Brigitte Szelies Wolf-Dieter Heiss | 2006 | Clinical Neurology and Neurosurgery2006，，1： | 1 |
| 16 | ,How did the foreland react? Yangtze foreland foldand - thrust belt deformation related to exhumation of the Dabie Shan ultrahigh-pressure continental crust(eastern China) 显示文摘 | Ratschbacher Lothar Hacker B R | 1999 | Terra Nova1999，11，： | 1 |
| 17 | The quantitative evaluation of etch pits in the cubic crystal system PartⅠ , Part Ⅱ 显示文摘 | LOTHAR I HORST R HELMUT H | 1969 | Practical Metallography1969，6，： | 1 |
| 18 | Reduced number of circulating endothelial progenitor cells predicts future cardiovascular events:proof of concept for the clinical importance of endogenous vascular repair显示文摘 | Caroline SL Lothar R Stephan F | 2005 | Circulation2005，111，： | 1 |
| 19 | Electron avalanches in oxygen and in mixtures of 02 and H2 : determination of the first townsend co- efficient a显示文摘 | Corbin R J Lothar F | 1974 | Physical Review A1974，10，6： | 1 |
| 20 | Enzymatic preparation of silybin phase II metabolites: sulfation using aryl sulfotransferase from rat liver显示文摘 | Kate?ina Purchartová Leonie Engels Petr Marhol Miroslav ?ulc Marek Kuzma Kristyna Slámová Lothar Elling Vladimír K?en | 2013 | Applied Microbiology and Biotechnology2013，，24： | 1 |
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