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| 1 | Characterization of Basic Nitrogen Aromatic Species Obtained during Fluid Catalytic Cracking by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry显示文摘The basic-nitrogen aromatic compounds in feedstocks and liquid products from the micro-reactor and soluble components of coke obtained during fluid catalytic cracking (FCC) process were analyzed by the micro-electrospray ionization (ESI) 9.4T Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) with an average mass resolving power of 300 000 at a mass range of 100-1 200. The analytical results revealed that the coker gas oil (CGO) contained a higher abundance of basic-nitrogen aromatic compounds with the type of -5N to -9N compared with those in deasphalted oil (DAO) and mixed FCC feedstock. After catalytic cracking, the abundance of lowly condensed basic-nitrogen aromatic compounds was much less than those of highly condensed aromatics in the liquid products, with the carbon number mainly ranging from 6 to 25 and the average carbon number of the side-chains equating to 1-5. On the contrary, with respect to the soluble components of coke, the abundance of lowly condensed basic-nitrogen aromatic compounds was more than those of highly condensed aromatics, and the carbon number ranged from 12 to 30, which was much smaller than that of the mixed FCC feedstock but slightly larger than that of the cracked liquid products. These results have provided some fundamental information on FCC process. | Liu Yingrong Wang Wei Hu Qiuling Zhu Yuxia Deng Jinghui Tian Songbai | 2012 | China Petroleum Processing & Petrochemical Technology2012，14，2： | 8 |
| 2 | Correlation between the cumulative analgesic effect of electroacupuncture intervention and synaptic plasticity of hypothalamic paraventricular nucleus neurons in rats with sciatica显示文摘In the present study, a rat model of chronic neuropathic pain was established by ligation of the sciatic nerve and a model of learning and memory impairment was established by ovariectomy to investigate the analgesic effect of repeated electroacupuncture stimulation at bilateral Zusanli (ST36) and Yanglingquan (GB34). In addition, associated synaptic changes in neurons in the paraventricular nucleus of the hypothalamus were examined. Results indicate that the thermal pain threshold (paw withdrawal latency) was significantly increased in rats subjected to 2-week electroacupuncture intervention compared with 2-day electroacupuncture, but the analgesic effect was weakened remarkably in ovariectomized rats with chronic constrictive injury. 2-week electroacupuncture intervention substantially reversed the chronic constrictive injury-induced increase in the synaptic cleft width and thinning of the postsynaptic density. These findings indicate that repeated electroacupuncture at bilateral Zusanli and Yanglingquan has a cumulative analgesic effect and can effectively relieve chronic neuropathic pain by remodeling the synaptic structure of the hypothalamic paraventricular nucleus. | Qiuling Xu Tao Liu Shuping Chen Yonghui Gao Junying Wang Lina Qiao Junling Liu | 2013 | Neural Regeneration Research2013，8，3： | 6 |
| 3 | Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2显示文摘Rescuing cells from stress damage emerges a potential therapeutic strategy to combat myocardial infarction.Protocatechuic aldehyde(PCA)is a major phenolic acid in Chinese herb Danshen(Salvia miltiorrhiza root).This study investigated whether PCA regulated nuclear pyruvate kinase isoform M2(PKM2)function to protect cardiomyocytes.In rats subjected to isoprenaline,PCA attenuated heart injury and protected cardiomyocytes from apoptosis.Through DARTS and CETSA assays,we identified that PCA bound and promoted PKM2 nuclear translocation in cardiomyocytes exposed to oxygen/glucose deprivation(OGD).In the nucleus,PCA increased the binding of PKM2 to β-Catenin via preserving PKM2 acetylation,and the complex,in cooperation with T-cell factor 4(TCF4),was required for transcriptional induction of genes encoding anti-apoptotic proteins,contributing to rescuing cardiomyocyte survival.In addition,PCA ameliorated mitochondrial dysfunction and prevented mitochondrial apoptosis dependent on PKM2.Consistently,PCA increased the binding of PKM2 to β-Catenin,improved heart contractive function,normalized heart structure and attenuated oxidative damage in mice subjected to artery ligation,but the protective effects were lost in Pkm2-deficient heart.Together,we showed that PCA regulated nuclear PKM2 function to rescue cardiomyocyte survival via β-Catenin/TCF4 signaling cascade,suggesting the potential of pharmacological intervention of PKM2 shuttle to protect the heart. | Xunxun Wu Lian Liu Qiuling Zheng Haiping Hao Hui Ye Ping Li Hua Yang | 2021 | Acta Pharmaceutica Sinica B2021，11，11： | 4 |
| 4 | Single-cell transcriptomic landscape of human blood cells显示文摘High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis.However,an elaborate and comprehensive transcriptome reference of the whole blood system is lacking.Here,we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types,including hematopoietic stem cells,progenitors and mature blood cells derived from 21 healthy donors.With high sequencing depth and coverage,we constructed a single-cell transcriptional atlas of blood cells(ABC) on the basis of both protein-coding genes and long noncoding RNAs(lncRNAs),and showed a high consistence between them.Notably,putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified.While common transcription factor regulatory networks were activated in neutrophils and monocytes,lymphoid cells dramatically changed their regulatory networks during differentiation.Furthermore,we showed a subset of nucleated erythrocytes actively expressing immune signals,suggesting the existence of erythroid precursors with immune functions.Finally,a web portal offering transcriptome browsing and blood cell type prediction has been established.Thus,our work provides a transcriptional map of human blood cells at single-cell resolution,thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis. | Xiaowei Xie Mengyao Liu Yawen Zhang Bingrui Wang Caiying Zhu Chenchen Wang Qing Li Yingying Huo Jiaojiao Guo Changlu Xu Linping Hu Aiming Pang Shihui Ma Una Wang Wenbin Cao Shulian Chen Qiuling Li Sudong Zhang Xueying Zhao Wen Zhou Hongbo Luo Guoguang Zheng Erlie Jiang Sizhou Feng Lixiang Chen Lihong Shi Hui Cheng Sha Hao Ping Zhu Tao Cheng | 2021 | National Science Review2021，8，3： | 3 |
| 5 | Enhancement of α-ketoisovalerate production by relieving the product inhibition of L-amino acid deaminase from Proteus mirabilis显示文摘L-Amino acid deaminase(LAAD) is a key enzyme in the deamination of L-valine(L-val) to produce α-ketoisovalerate(KIV). However, the product inhibition of LAAD is a major hindrance to industrial KIV production.In the present study, a combination strategy of modification of flexible loop regions around the product binding site and the avoidance of dramatic change of main-chain dynamics was reported to reduce the product inhibition.The four mutant PM-LAAD^(M4)(PM-LAAD^(S98A/T105A/S106A/L341A)) achieved a 6.2-fold higher catalytic efficiency and an almost 6.7-fold reduction in product inhibition than the wild-type enzyme. Docking experiments suggested that weakened interactions between the product and enzyme, and the flexibility of the 'lid' structure relieved LAAD product inhibition. Finally, the whole-cell biocatalyst PM-LAAD^(M4) has been applied to KIV production,the titer and conversion rate of KIV from L-val were 98.5 g·L^-1 and 99.2% at a 3-L scale, respectively. These results demonstrate that the newly engineered catalyst can significantly reduce the product inhibition, that making KIV a prospective product by bioconversion method, and also provide the understanding of the mechanism of the relieved product inhibition of PM-LAAD. | Shanshan Pei Xiaobo Ruan Jia Liu Wei Song Xiulai Chen Qiuling Luo Liming Liu Jing Wu | 2020 | Chinese Journal of Chemical Engineering2020，28，8： | 2 |
| 6 | Significance and strategies in developing delivery systems for bio-macromolecular drugs显示文摘 | Huining HE Qiuling LIANG Meong Cheoi SHIN Kyuri LEE Junbo GONG Junxiao YE Quan LIU Jingkang WANG Victor YANG | 2013 | Frontiers of Chemical Science and Engineering2013，7，4： | 2 |
| 7 | GM130 regulates pulmonary surfactant protein secretion in alveolar typeⅡcells显示文摘Pulmonary surfactant is a lipid-protein complex secreted by alveolar typeⅡepithelial cells and is essential for the maintenance of the delicate structure of mammalian alveoli to promote efficient gas exchange across the air-liquid barrier.The Golgi apparatus plays an important role in pulmonary surfactant modification and secretory trafficking.However,the physiological function of the Golgi apparatus in the transport of pulmonary surfactants is unclear.In the present study,deletion of GM130,which encodes for a matrix protein of the cis-Golgi cisternae,was shown to induce the disruption of the Golgi structure leading to impaired secretion of lung surfactant proteins and lipids.Specifically,the results of in vitro and in vivo analysis indicated that the loss of GM130 resulted in trapping of Sftpa in the endoplasmic reticulum,Sftpb and Sftpc accumulation in the Golgi apparatus,and an increase in the compensatory secretion of Sftpd.Moreover,global and epithelial-specific GM130 knockout in mice resulted in an enlargement of alveolar airspace and an increase in alveolar epithelial autophagy;however,surfactant repletion partially rescued the enlarged airspace defects in GM130-deficient mice.Therefore,our results demonstrate that GM130 and the mammalian Golgi apparatus play a critical role in the control of surfactant protein secretion in pulmonary epithelial cells. | Qianqian Pang Chunyi Liu Yulong Qiao Jian Zhao Sin Man Lam Mei Mei Guanghou Shui Shilai Bao Qiuling Li | 2022 | Science China(Life Sciences)2022，65，1： | 2 |
| 8 | An essential role for PNLDC1 in piRNA 3' end trimming and male fertility in mice显示文摘 | Yue Zhang Rui Guo Yiqiang Cui Zhiping Zhu Yingwen Zhang Hao Wu Bo Zheng Qiuling Yue Shun Bai Wentao Zeng Xuejiang Guo Zuomin Zhou Bin Shen Ke Zheng Mingxi Liu Lan Ye Jiahao Sha | 2017 | Cell Research2017，27，11： | 2 |
| 9 | Preparation and doping mode of doped LiMn2O4 for Li-Ion batteries显示文摘 | LIU Qiuling WANG Shengping TAN Haibo | 2013 | Energies2013，6，3： | 1 |
| 10 | High rate ca-pabilities Fe-doped EMD electrodes for Li/MnO2 primary battery 显示文摘 | Liu Qiuling Wang Shengping Cheng Hong | 2013 | International Journal of Electrochemical Sci- ence2013，8，10： | 1 |
| 11 | Simultaneous Quantification of Seven Main Triterpenoid Saponins in Radix et Rhizoma Clematidis by LC–ELSD显示文摘 | Xiaoli Ma Le Xie Lifang Liu Qiuling Tang Zhuqing Wan Yuqing Li | 2009 | Chromatographia (-)2009，，5： | 1 |
| 12 | A high-resolution cell atlas of the domestic pig lung and an online platform for exploring lung single-cell data显示文摘The genetically engineered pig is regarded as an optimal source of organ transplantation for humans and an excellent model for human disease research,given its comparable physiology to human beings.A myriad of single-cell RNA sequencing(sc RNA-seq)data on humans has been reported,but such data on pigs are scarce.Here,we apply sc RNA-seq technology to study the cellular heterogeneity of 3-month-old pig lungs,generating the single-cell atlas of 13,580 cells covering 16 major cell types.Based on these data,we systematically characterize the similarities and differences in the cellular cross-talk and expression patterns of respiratory virus receptors in each cell type of pig lungs compared with human lungs.Furthermore,we analyze pig lung xenotransplantation barriers and reported the cell-type expression patterns of 10 genes associated with pig-to-human immunobiological incompatibility and coagulation dysregulation.We also investigate the conserved transcription factors(TFs)and their candidate target genes and constructed five conserved TF regulatory networks in the main cell types shared by pig and human lungs.Finally,we present a comprehensive and openly accessible online platform,Scdb Lung.Our sc RNA-seq atlas of the domestic pig lung and Scdb Lung database can guide pig lung research and clinical applicability. | Lijing Zhang Jiacheng Zhu Haoyu Wang Jun Xia Ping Liu Fang Chen Hui Jiang Qiuling Miao Weiying Wu Lingli Zhang Lihua Luo Xiaosen Jiang Yong Bai Chengcheng Sun Dongsheng Chen Xingliang Zhang | 2021 | Journal of Genetics and Genomics2021，48，5： | 1 |
| 13 | Anisotropic Expansion and High Rate Discharge Performance of V-doped MnO2for Li/MnO2 Primary Battery显示文摘 | Wang Shengping Liu Qiuling | 2012 | Int J Electrochem Sci2012，7，2： | 1 |
| 14 | Dihydrotanshinone I preconditions myocardium against ischemic injury via PKM2 glutathionylation sensitive to ROS显示文摘Ischemic preconditioning(IPC)is a potential intervention known to protect the heart against ischemia/reperfusion injury,but its role in the no-reflow phenomenon that follows reperfusion is unclear.Dihydrotanshinone I(DT)is a natural compound and this study illustrates its role in cardiac ischemic injury from the aspect of IPC.Pretreatment with DT induced modest ROS production and protected cardiomyocytes against oxygen and glucose deprivation(OGD),but the protection was prevented by a ROS scavenger.In addition,DT administration protected the heart against isoprenaline challenge.Mechanistically,PKM2 reacted to transient ROS via oxidization at Cys423/Cys424,leading to glutathionylation and nuclear translocation in dimer form.In the nucleus,PKM2 served as a co-factor to promote HIF-1a-dependent gene induction,contributing to adaptive responses.In mice subjected to permanent coronary ligation,cardiac-specific knockdown of Pkm2 blocked DT-mediated preconditioning protection,which was rescued by overexpression of wild-type Pkm2,rather than Cys423/424-mutated Pkm2.In conclusion,PKM2 is sensitive to oxidation,and subsequent glutathionylation promotes its nuclear translocation.Although IPC has been viewed as a protective means against reperfusion injury,our study reveals its potential role in protection of the heart from no-reflow ischemia. | Xunxun Wu Lian Liu Qiuling Zheng Hui Ye Hua Yang Haiping Hao Ping Li | 2023 | Acta Pharmaceutica Sinica B2023，13，1： | 1 |
| 15 | Six novel single-nucleotide polymorphisms in SPAG11 gene and their association with sperm quality traits in Chinese Holstein bulls显示文摘 | Xinyan Liu Zhihua Ju Lingling Wang Yan Zhang Jingmin Huang Qiuling Li Jianbin Li Jifeng Zhong Liguo An Changfa Wang | 2011 | Animal Reproduction Science2011，，1： | 1 |
| 16 | A novel Physarum polycephalum SR protein kinase specifically phosphorylates the RS domain of the human SR protein, ASF/SF2显示文摘象蛋白质一样的充满丝氨酸的蛋白质 kinase (SRPK ) 的 A 1591-bp cDNA 在 Physarum polycephalum 被识别了(GenBank 同意没有。DQ140379 ) 。cDNA 在 bp 包含二个重复序列 1153 并且 bp 395547。编码顺序是 56% 对人的 SRPK1 相应并且被称为 Physarum SRPK (PSRPK ) 。与另外的 SRPK 一致, PSRPK 的一致主题在二保存的域(CD ) 以内。然而,在 N 终端和 CD 之间的分叉的主题比另外的 SRPK 的相应序列短得多。学习这蛋白质的结构和功能,我们在 Escherichia coli 并且在 vitro phosphorylation 试金执行了合作表示实验在人的 SR 蛋白质上调查 recombinant PSRPK 的 phosphorylation 效果, ASF/SF2。西方的污点分析显示出那 PSRPK 能在 E 的 phosphorylate ASF/SF2。coli 房间。Autoradiographic 检查显示出那 recombinant PSRPK 和有在 N 终点的 28-aa 删除的 PSRPK 的一种截断的形式能 phosphorylate ASF/SF2 和包含 RS 领域的 ASF/SF2 的一种截断的形式。然而,这二 PSRPK 形成不能 phosphorylate 缺乏 RS 领域的截断的形式 ASF/SF2。也 CD 缺乏的 PSRPK 的一种截断的形式不举办任何 phosphorylation 活动。这些结果显示在 PSRPK 的 phosphorylation 地点象另外的 SRPK 一样,位于 SR 蛋白质的 RS 领域以内并且它的 phosphorylation 活动仔细与二 CD 被联系。PSRPK 的结构和功能上的这研究证明它是 SRPK 家庭的一个新成员。 | Shide Liu Kang Kang Jianhua Zhang Qiuling Ouyang Zhuolong Zhou Shengli Tian Miao Xing | 2009 | Acta Biochimica et Biophysica Sinica2009，41，8： | 1 |
| 17 | Ultrasound diagnosis about branchial cyst in parotid显示文摘 | Shi Qiuling Hui Luo Yang Jiao Tao Liu Jun Lu | 2014 | International English Education Research2014，，2： | 0 |
| 18 | The haplotype-resolved T2T genome of teinturier cultivar Yan73 reveals the genetic basis of anthocyanin biosynthesis in grapes显示文摘Teinturier grapes are characterized by the typical accumulation of anthocyanins in grape skin,flesh,and vegetative tissues,endowing them with high utility value in red wine blending and nutrient-enriched foods developing.However,due to the lack of genome information,the mechanism involved in regulating teinturier grape coloring has not yet been elucidated and their genetic utilization research is still insufficient.Here,the cultivar‘Yan73’was used for assembling the telomere-to-telomere(T2T)genome of teinturier grapes by combining the High Fidelity(HiFi),Hi-C and ultralong Oxford Nanopore Technologies(ONT)reads.Two haplotype genomes were assembled,at the sizes of 501.68 Mb and 493.38 Mb,respectively.In the haplotype 1 genome,the transposable elements(TEs)contained 32.77%of long terminal repeats(LTRs),while in the haplotype 2 genome,31.53%of LTRs were detected in TEs.Furthermore,obvious inversions were identified in chromosome 18 between the two haplotypes.Transcriptome profiling suggested that the gene expression patterns in‘Cabernet Sauvignon’and‘Yan73’were diverse depending on tissues,developmental stages,and varieties.The transcription program of genes in the anthocyanins biosynthesis pathway between the two cultivars exhibited high similarity in different tissues and developmental stages,whereas the expression levels of numerous genes showed significant differences.Compared with other genes,the expression levels of VvMYBA1 and VvUFGT4 in all samples,VvCHS2 except in young shoots and VvPAL9 except in the E-L23 stage of‘Yan73’were higher than those of‘Cabernet Sauvignon’.Further sequence alignments revealed potential variant gene loci and structure variations of anthocyanins biosynthesis related genes and a 816 bp sequence insertion was found in the promoter of VvMYBA1 of‘Yan73’haplotype 2 genome.The‘Yan73’T2T genome assembly and comparative analysis provided valuable foundations for further revealing the coloring mechanism of teinturier grapes and the genetic improvement of grape coloring traits. | Kekun Zhang Mengrui Du Hongyan Zhang Xiaoqian Zhang Shuo Cao Xu Wang Wenrui Wang Xueqiang Guan Penghui Zhou Jin Li Wenguang Jiang Meiling Tang Qiuling Zheng Muming Cao Yongfeng Zhou Keqin Chen Zhongjie Liu Yulin Fang | 2023 | Horticulture Research2023，10，11： | 0 |
| 19 | A chromosome-level genome assembly for Dracaena cochinchinensis reveals the molecular basis of its longevity and formation of dragon’s blood显示文摘Dracaena,a remarkably long-lived and slowly maturing species of plant,is world famous for its ability to produce dragon’s blood,a precious traditional medicine used by different cultures since ancient times.However,there is no detailed and high-quality genome available for this species at present;thus,the molecular mechanisms that underlie its important traits are largely unknown.These factors seriously limit the protection and regeneration of this rare and endangered plant resource.Here,we sequenced and assembled the genome of Dracaena cochinchinensis at the chromosome level.The D.cochinchinensis genome covers 1.21 Gb with a scaffold N50 of 50.06 Mb and encodes 31619 predicted protein-coding genes.Analysis showed that D.cochinchinensis has undergone two whole-genome duplications and two bursts of long terminal repeat insertions.The expansion of two gene classes,cis-zeatin O-glucosyltransferase and small auxin upregulated RNA,were found to account for its longevity and slow growth.Two transcription factors(bHLH and MYB)were found to be core regulators of the flavonoid biosynthesis pathway,and reactive oxygen species were identified as the specific signaling molecules responsible for the injuryinduced formation of dragon’s blood.Our study provides high-quality genomic information relating to D.cochinchinensis and significant insight into the molecular mechanisms responsible for its longevity and formation of dragon’s blood.These findings will facilitate resource protection and sustainable utilization of Dracaena. | Yanhong Xu Kaijian Zhang Zhonglian Zhang Yang Liu Feifei Lv Peiwen Sun Shixi Gao Qiuling Wang Cuicui Yu Jiemei Jiang Chuangjun Li Meifang Song Zhihui Gao Chun Sui Haitao Li Yue Jin Xinwei Guo Jianhe Wei | 2022 | Plant Communications2022，3，6： | 0 |
| 20 | Drug target discovery by magnetic nanoparticles coupled mass spectrometry显示文摘Drug target discovery is the basis of drug screening.It elucidates the cause of disease and the mechanism of drug action,which is the essential of drug innovation.Target discovery performed in biological systems is complicated as proteins are in low abundance and endogenous compounds may interfere with drug binding.Therefore,methods to track drug-target interactions in biological matrices are urgently required.In this work,a Fe_(3)O_(4) nanoparticle-based approach was developed for drug-target screening in biofluids.A known ligand-protein complex was selected as a principle-to-proof example to validate the feasibility.After incubation in cell lysates,ligand-modified Fe_(3)O_(4) nanoparticles bound to the target protein and formed complexes that were separated from the lysates by a magnet for further analysis.The large surface-to-volume ratio of the nanoparticles provides more active sites for the modification of chemical drugs.It enhances the opportunity for ligand-protein interactions,which is beneficial for capturing target proteins,especially for those with low abundance.Additionally,a one-step magnetic separation simplifies the pre-processing of ligand-protein complexes,so it effectively reduces the endogenous interference.Therefore,the present nanoparticle-based approach has the potential to be used for drug target screening in biological systems. | Dandan Xia Baoling Liu Xiaowei Xu Ya Ding Qiuling Zheng | 2021 | Journal of Pharmaceutical Analysis2021，11，1： | 0 |
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