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| 1 | Correlation between the cumulative analgesic effect of electroacupuncture intervention and synaptic plasticity of hypothalamic paraventricular nucleus neurons in rats with sciatica显示文摘In the present study, a rat model of chronic neuropathic pain was established by ligation of the sciatic nerve and a model of learning and memory impairment was established by ovariectomy to investigate the analgesic effect of repeated electroacupuncture stimulation at bilateral Zusanli (ST36) and Yanglingquan (GB34). In addition, associated synaptic changes in neurons in the paraventricular nucleus of the hypothalamus were examined. Results indicate that the thermal pain threshold (paw withdrawal latency) was significantly increased in rats subjected to 2-week electroacupuncture intervention compared with 2-day electroacupuncture, but the analgesic effect was weakened remarkably in ovariectomized rats with chronic constrictive injury. 2-week electroacupuncture intervention substantially reversed the chronic constrictive injury-induced increase in the synaptic cleft width and thinning of the postsynaptic density. These findings indicate that repeated electroacupuncture at bilateral Zusanli and Yanglingquan has a cumulative analgesic effect and can effectively relieve chronic neuropathic pain by remodeling the synaptic structure of the hypothalamic paraventricular nucleus. | Qiuling Xu Tao Liu Shuping Chen Yonghui Gao Junying Wang Lina Qiao Junling Liu | 2013 | Neural Regeneration Research2013，8，3： | 6 |
| 2 | Machine learning for perovskite materials design and discovery显示文摘The development of materials is one of the driving forces to accelerate modern scientific progress and technological innovation.Machine learning(ML)technology is rapidly developed in many fields and opening blueprints for the discovery and rational design of materials.In this review,we retrospected the latest applications of ML in assisting perovskites discovery.First,the development tendency of ML in perovskite materials publications in recent years was organized and analyzed.Second,the workflow of ML in perovskites discovery was introduced.Then the applications of ML in various properties of inorganic perovskites,hybrid organic–inorganic perovskites and double perovskites were briefly reviewed.In the end,we put forward suggestions on the future development prospects of ML in the field of perovskite materials. | Qiuling Tao Pengcheng Xu Minjie Li Wencong Lu | 2021 | npj Computational Materials2021，，1： | 4 |
| 3 | Single-cell transcriptomic landscape of human blood cells显示文摘High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis.However,an elaborate and comprehensive transcriptome reference of the whole blood system is lacking.Here,we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types,including hematopoietic stem cells,progenitors and mature blood cells derived from 21 healthy donors.With high sequencing depth and coverage,we constructed a single-cell transcriptional atlas of blood cells(ABC) on the basis of both protein-coding genes and long noncoding RNAs(lncRNAs),and showed a high consistence between them.Notably,putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified.While common transcription factor regulatory networks were activated in neutrophils and monocytes,lymphoid cells dramatically changed their regulatory networks during differentiation.Furthermore,we showed a subset of nucleated erythrocytes actively expressing immune signals,suggesting the existence of erythroid precursors with immune functions.Finally,a web portal offering transcriptome browsing and blood cell type prediction has been established.Thus,our work provides a transcriptional map of human blood cells at single-cell resolution,thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis. | Xiaowei Xie Mengyao Liu Yawen Zhang Bingrui Wang Caiying Zhu Chenchen Wang Qing Li Yingying Huo Jiaojiao Guo Changlu Xu Linping Hu Aiming Pang Shihui Ma Una Wang Wenbin Cao Shulian Chen Qiuling Li Sudong Zhang Xueying Zhao Wen Zhou Hongbo Luo Guoguang Zheng Erlie Jiang Sizhou Feng Lixiang Chen Lihong Shi Hui Cheng Sha Hao Ping Zhu Tao Cheng | 2021 | National Science Review2021，8，3： | 3 |
| 4 | Estrogen improved metabolic syndrome through down-regulation of VEGF and HIF-1α to inhibit hypoxia of periaortic and intra-abdominal fat in ovariectomized female rats显示文摘 | JinWen Xu QiuLing Xiang GuiPing Lin XiaoDong Fu KeWen Zhou Ping Jiang ShuHui Zheng TingHuai Wang | 2012 | Molecular Biology Reports2012，，8： | 1 |
| 5 | Hydrogen selenide,a vital metabolite of sodium selenite,uncouples the sulfilimine bond and promotes the reversal of liver fibrosis显示文摘Sodium selenite has alleviating effects on liver fibrosis;however,its therapeutic molecular mechanism remains unclear.Herein,hydrogen selenide,a major metabolite of Na_(2)SeO_(3),was tested to uncouple the sulfilimine bond in collagen IV,the biomarker of liver fibrosis.A mouse model of liver fibrosis was constructed via a CCl_(4)-induced method,followed by the administration of 0.2 mg kg−1 Na_(2)SeO_(3)via gavage three times per week for 4 weeks.Changes in H2Se,NADPH,and H_(2)O_(2)levels were monitored in real time by using NIR-H2Se,DCI-MQ-NADPH,and H_(2)O_(2)probes in vivo,respectively.H_(2)Se continuously accumulated in the liver throughout the Na_(2)SeO_(3)treatment period,but the levels of NADPH and H_(2)O_(2)decreased.The expression of collagen IV was analyzed through Western blot and liquid chromatography-mass spectrometry.Results confirmed that the sulfilimine bond of collagen IV in the fibrotic mouse livers could be broken by H2Se with the Na_(2)SeO_(3)treatment.Therefore,the therapeutic effect of Na_(2)SeO_(3)on liver fibrosis could be mainly attributed to H_(2)Se that uncoupled the sulfilimine bond to induce collagen IV degradation.This study provided a reasonable explanation for the molecular mechanism of the in vivo function of Na_(2)SeO_(3)and the prevention of liver fibrosis by administering inorganic selenium. | Dongrui Luan Zengteng Zhao Dandan Xia Qiuling Zheng Xiaonan Gao Kehua Xu Bo Tang | 2021 | Science China(Life Sciences)2021，64，3： | 1 |
| 6 | Expedient chemoselective and catalyst-free synthesis of 3,3-difluorochroman-4-ones from o-hydroxyarylenaminones and Selectfluor显示文摘An expedient and mild strategy for the synthesis of unconventional 2-(dimethylamino)-3,3-difluorochroman-4-one derivatives from o-hydroxyarylenaminones and Selectfluor was developed at room temperature under catalyst-free conditions. This method showed excellent chemoselectivity and great functional groups tolerance. | Jian Xu Zhijie Kuang Qiuling Song | 2018 | Chinese Chemical Letters2018，29，6： | 0 |
| 7 | Design,Synthesis,and Applications of ortho-Sulfur Substituted Arylphosphanes显示文摘Ortho-sulfur substituted arylphosphine oxides and arylphosphonates are the precursors of orthosulfurated arylphosphanes,which are widely utilized as ligands in transition metal-catalyzed reactions.However,efficient synthetic methods to access such compounds are sparse.Herein,highly valuable ortho-sulfur substituted arylphosphine oxides and arylphosphonates were constructed via difunctionalization of aryne precursors with P(O)H and elemental sulfur.This method avoids the use of transition metal catalysts and corrosive phosphorus chloride.The synthetic utility of this reaction is further demonstrated by the reduction of the synthesized products to access monophosphine ligands and their applications in cross-coupling reactions.Moreover,by introducing an alkyne moiety into the P(O)H substrate,cyclic sulfur-containing phosphines could be obtained in a one-pot reaction,which represents a new P,S structure that is inaccessible by known strategies. | Yu Guo Ying Luo Shiqiang Mu Jianke Su Jian Xu Qiuling Song | 2023 | CCS Chemistry2023，5，6： | 0 |
| 8 | The haplotype-resolved T2T genome of teinturier cultivar Yan73 reveals the genetic basis of anthocyanin biosynthesis in grapes显示文摘Teinturier grapes are characterized by the typical accumulation of anthocyanins in grape skin,flesh,and vegetative tissues,endowing them with high utility value in red wine blending and nutrient-enriched foods developing.However,due to the lack of genome information,the mechanism involved in regulating teinturier grape coloring has not yet been elucidated and their genetic utilization research is still insufficient.Here,the cultivar‘Yan73’was used for assembling the telomere-to-telomere(T2T)genome of teinturier grapes by combining the High Fidelity(HiFi),Hi-C and ultralong Oxford Nanopore Technologies(ONT)reads.Two haplotype genomes were assembled,at the sizes of 501.68 Mb and 493.38 Mb,respectively.In the haplotype 1 genome,the transposable elements(TEs)contained 32.77%of long terminal repeats(LTRs),while in the haplotype 2 genome,31.53%of LTRs were detected in TEs.Furthermore,obvious inversions were identified in chromosome 18 between the two haplotypes.Transcriptome profiling suggested that the gene expression patterns in‘Cabernet Sauvignon’and‘Yan73’were diverse depending on tissues,developmental stages,and varieties.The transcription program of genes in the anthocyanins biosynthesis pathway between the two cultivars exhibited high similarity in different tissues and developmental stages,whereas the expression levels of numerous genes showed significant differences.Compared with other genes,the expression levels of VvMYBA1 and VvUFGT4 in all samples,VvCHS2 except in young shoots and VvPAL9 except in the E-L23 stage of‘Yan73’were higher than those of‘Cabernet Sauvignon’.Further sequence alignments revealed potential variant gene loci and structure variations of anthocyanins biosynthesis related genes and a 816 bp sequence insertion was found in the promoter of VvMYBA1 of‘Yan73’haplotype 2 genome.The‘Yan73’T2T genome assembly and comparative analysis provided valuable foundations for further revealing the coloring mechanism of teinturier grapes and the genetic improvement of grape coloring traits. | Kekun Zhang Mengrui Du Hongyan Zhang Xiaoqian Zhang Shuo Cao Xu Wang Wenrui Wang Xueqiang Guan Penghui Zhou Jin Li Wenguang Jiang Meiling Tang Qiuling Zheng Muming Cao Yongfeng Zhou Keqin Chen Zhongjie Liu Yulin Fang | 2023 | Horticulture Research2023，10，11： | 0 |
| 9 | A chromosome-level genome assembly for Dracaena cochinchinensis reveals the molecular basis of its longevity and formation of dragon’s blood显示文摘Dracaena,a remarkably long-lived and slowly maturing species of plant,is world famous for its ability to produce dragon’s blood,a precious traditional medicine used by different cultures since ancient times.However,there is no detailed and high-quality genome available for this species at present;thus,the molecular mechanisms that underlie its important traits are largely unknown.These factors seriously limit the protection and regeneration of this rare and endangered plant resource.Here,we sequenced and assembled the genome of Dracaena cochinchinensis at the chromosome level.The D.cochinchinensis genome covers 1.21 Gb with a scaffold N50 of 50.06 Mb and encodes 31619 predicted protein-coding genes.Analysis showed that D.cochinchinensis has undergone two whole-genome duplications and two bursts of long terminal repeat insertions.The expansion of two gene classes,cis-zeatin O-glucosyltransferase and small auxin upregulated RNA,were found to account for its longevity and slow growth.Two transcription factors(bHLH and MYB)were found to be core regulators of the flavonoid biosynthesis pathway,and reactive oxygen species were identified as the specific signaling molecules responsible for the injuryinduced formation of dragon’s blood.Our study provides high-quality genomic information relating to D.cochinchinensis and significant insight into the molecular mechanisms responsible for its longevity and formation of dragon’s blood.These findings will facilitate resource protection and sustainable utilization of Dracaena. | Yanhong Xu Kaijian Zhang Zhonglian Zhang Yang Liu Feifei Lv Peiwen Sun Shixi Gao Qiuling Wang Cuicui Yu Jiemei Jiang Chuangjun Li Meifang Song Zhihui Gao Chun Sui Haitao Li Yue Jin Xinwei Guo Jianhe Wei | 2022 | Plant Communications2022，3，6： | 0 |
| 10 | Drug target discovery by magnetic nanoparticles coupled mass spectrometry显示文摘Drug target discovery is the basis of drug screening.It elucidates the cause of disease and the mechanism of drug action,which is the essential of drug innovation.Target discovery performed in biological systems is complicated as proteins are in low abundance and endogenous compounds may interfere with drug binding.Therefore,methods to track drug-target interactions in biological matrices are urgently required.In this work,a Fe_(3)O_(4) nanoparticle-based approach was developed for drug-target screening in biofluids.A known ligand-protein complex was selected as a principle-to-proof example to validate the feasibility.After incubation in cell lysates,ligand-modified Fe_(3)O_(4) nanoparticles bound to the target protein and formed complexes that were separated from the lysates by a magnet for further analysis.The large surface-to-volume ratio of the nanoparticles provides more active sites for the modification of chemical drugs.It enhances the opportunity for ligand-protein interactions,which is beneficial for capturing target proteins,especially for those with low abundance.Additionally,a one-step magnetic separation simplifies the pre-processing of ligand-protein complexes,so it effectively reduces the endogenous interference.Therefore,the present nanoparticle-based approach has the potential to be used for drug target screening in biological systems. | Dandan Xia Baoling Liu Xiaowei Xu Ya Ding Qiuling Zheng | 2021 | Journal of Pharmaceutical Analysis2021，11，1： | 0 |
| 11 | Global analysis of basic leucine zipper transcription factors in trifoliate orange and the function identification of PtbZIP49 in salt tolerance显示文摘As one of the most widely distributed and highly conserved transcription factors in eukaryotes,basic leucine zipper proteins(bZIPs)are involved in a variety of biological processes in plants,but they are largely unknown in citrus.In this study,56 bZIP family members were identified genome-wide from an important citrus rootstock,namely trifoliate orange(Poncirus trifoliata L.Raf.),and these putative bZIPs were named PtbZIP1—PtbZIP56.All PtbZIPs were classified into 13 subgroups by phylogenetic comparison with Arabidopsis thaliana bZIPs(AtbZIPs),and they were randomly distributed on nine known(50 genes)chromosomes and one unknown(6 genes)chromosome.Sequence analysis revealed the detailed characteristics of PtPZIPs,including their amino acid length,isoelectric point(pI),molecular weight(MW),predicted subcellular localization,gene structure,and conserved motifs.Prediction of promoter elements suggested the presence of drought,low-temperature,wound,and defense and stress responsive elements,as well as multiple hormone-responsive cis-acting elements.Spatiotemporal expression analysis showed the transcriptional patterns of PtbZIPs in different tissues and under dehydration,high salt,ABA,and IAA treatments.In addition,21 PtbZIPs were predicted to have direct or indirect protein—protein interactions.Among these,PtbZIP49 was experimentally proven to interact with PtbZIP1 or PtbZIP11 by using a yeast two-hybrid assay and bimolecular fluorescence complementation(BiFC).Subcellular localization analysis further revealed that PtbZIP1,PtbZIP11,and PtbZIP49 were localized in the nucleus.Moreover,PtbZIP49 was functionally identified as having an important role in salt stress via ectopic expression in A.thaliana and silenced in trifoliate orange using virus-induced gene silencing(VIGS).This study provided comprehensive information on PtbZIP transcription factors in citrus and highlights their potential functions in abiotic stress. | Yuanyuan Xu Qiuling Hui Meng Li Hongxian Peng Yizhong He Changpin Chun Liangzhi Peng Xingzheng Fu | 2024 | Horticultural Plant Journal2024，10，1： | 0 |
| 12 | Microfluidic magnetic detection system combined with a DNA framework-mediated immune-sandwich assay for rapid and sensitive detection of tumor-derived exosomes显示文摘Tumor-derived circulating exosomes(TDEs)are being pursued as informative and noninvasive biomarkers.However,quantitatively detecting TDEs is still challenging.Herein,we constructed a DNA tetrahedral-structured probe(TSP)-mediated microfluidic magnetic detection system(μFMS)to provide a rapid and sensitive platform for analyzing TDEs.CD63 aptamer-modified Fe_(3)O_(4)magnetic nanoparticles(MNPs)were constructed to form magnetic nano-report probes(MNRs).The microfluidic chips were fabricated from glass functionalized with DNA TSP-modified aldehyde groups and a PDMS layer designed with serpentine microchannels.An induction coil-based magnetic detector was used to measure the magnetic signal.The linear dynamic range of theμFMS system for TDE assays was 1.98×10^(3)-1.98×10^(7)particles/mL with a limit of detection of 1.98×10^(3)particles/mL in PBS.There was no significant difference in TDE detection between the simulated serum and PBS,which indicated the feasibility of the constructedμFMS system for TDE analysis in complex biological systems.In terms of cost,reaction time and operation procedure,thisμFMS has the potential to be developed as a clinical point-of-care testing tool for cancer diagnosis and therapeutics. | Qiuling Qian Yutong Wei Yi Xu Mengmeng Zheng Chenguang Wang Shulin Zhang Xiaoming Xie Chaofeng Ye Xianqiang Mi | 2023 | Microsystems & Nanoengineering2023，9，6： | 0 |
| 13 | Atom Recombination of Difluorocarbene Enables 3-Fluorinated Oxindoles from 2-Aminoarylketones显示文摘Contrary to the traditional implementation as a difluoromethyl group and recently disclosed role of C1 synthons in synthetic organic chemistry,difluorocarbene(:CF_(2))is reported herein to proceed in unprecedented atom recombination as both a C1 synthon and F1 reagent simultaneously to render valuable 3-fluorinated oxindoles from 2-aminoarylketones.The reaction does not require catalyst and features a broad range of substrates with good functional group compatibility and ease of execution.This transformation could be employed to the quick-constructions of certain bioactive molecule derivatives.The mechanistic experiments and density functional theory(DFT)calculations indicate that this atom recombination reaction of:CF_(2) for the synthesis of 3-fluorinated oxindoles may involve a rearrangement process of epoxide intermediates. | Guan Zhang Qianqian Shi Mengyuan Hou Kai Yang Shihui Wang Shuai Wang Wangyang Li Chaokun Li Jian Qiu Hetao Xu Lu Zhou Cece Wang Shi-Jun Li Yu Lan Qiuling Song | 2022 | CCS Chemistry2022，4，5： | 0 |
| 14 | Molecular imaging:design mechanism and bioapplications显示文摘Molecular imaging is a non-invasive method to image and analyze the concentration and activity of functional biomolecules in cells or in vivo at molecular level,and plays an increasing role in deep understanding of biological processes,early and accurate diagnosis of diseases,and evaluation of treatment.Nowadays,numerous novel molecular imaging probes have been developed,involving every biomedical imaging modality,such as optical imaging,photoacoustic imaging,magnetic resonance imaging,single-photon-emission computed tomography,and positron emission tomography.In this review,we summarize the development of current state-of-the-art molecular imaging probes.We introduce the design strategies of molecular probes and detailed imaging modalities,and highlight the properties of probes and biomedical imaging applications in cells and in vivo,including disease diagnosis,drug tracking,and imaging-guided surgery.Then we discuss the perspectives and challenges in this emerging field.We expect this review could inspire more effective molecular imaging probes to be developed,achieving the goal towards clinical practices. | Lanlan Chen Yifan Lyu Xuan Zhang Liting Zheng Qingqing Li Ding Ding Fengming Chen Yihao Liu Wei Li Yutong Zhang Qiuling Huang Zhiqiang Wang Tiantian Xie Qiang Zhang Yingyu Sima Ke Li Shuai Xu Tianbing Ren Mengyi Xiong Ying Wu Jibin Song Lin Yuan Huanghao Yang Xiao-Bing Zhang Weihong Tan | 2023 | Science China Chemistry2023，66，5： | 0 |
| 15 | Effect of thickness on infrared optical property of VO2 film deposited by magnetron sputtering显示文摘A series of VO2 films with different thicknesses(from about 25 nm to 250 nm)were prepared on sapphire substrates by radio frequency magnetron sputtering.The deposition times varied from 15 min to 150 min.The metal to insulator transitions(MIT)of the films were studied.The optical transmittance of the films to infrared light(with a wavelength of 4.0μm)at room temperature(30℃)varied significantly with film thickness,ranging from 86.53%to 41.01%.The modulation property also changes with thickness,decreasing from 22.89%to 14.74%.The phase transition temperature remained approximately 70℃during heating,and approximately 53℃during cooling,despite a tenfold increase in the deposition time,and the resulting thickness of the films.Raman spectroscopy of the films indicated that the intensities of the characteristic peaks corresponding to V2O5 increase with the increasing of film thickness.Temperature-dependent Raman spectroscopy indicated that the peaks corresponding to VO2 undergo reversible changes during heating and cooling of the films,while the peaks corresponding to V2O5 remain unchanged throughout.Careful control of the V2O5 content of the films(by varying the duration of the deposition process)allows control over their transmittance and optical modulation properties without changes in the phase transition temperature.This provides a new method of controlling the optical properties of these materials and shows promise for their potential applications in thermochromic windows. | YANG ZhenHuai YANG QiuLing YANG Lei DAI Bing XIA Fei WANG Peng GUO Shuai GAO Gang XU LiangGe ZHANG YuMin ZHU JiaQi | 2020 | Science China(Technological Sciences)2020，63，8： | 0 |
| 16 | Preparation of Iron-Pillared Bentonite/Oyster Shell Composite and Phosphate Adsorption in Water显示文摘Iron-pillared bentonite(FB)was prepared by Fe(III)modified bentonite,and then the composites(FB-OS)were prepared by iron-pillared bentonite and oyster shell powder.The composites were characterized by FTIR,SEM,TGA,and EDS,and the phosphorus removal test was carried out.The results showed that FB-OS contained a large amount of CaO.Its structure was compact,but there were gaps in it.The maximum bending stress and compressive strength were 43.7 N and 0.927 MPa,respectively.The phosphorus removal test showed that the phosphorus removal rate of FB-OS was more than 90%,and measured the maximum adsorption capacity was 48.31 mg/g.A large amount of spherical products were produced on the surface and inside of FB-OS after phos-phorus removal,it was speculated that spherical products were amorphous calcium phosphate in the paper.Ana-lysis indicated that there was chemical adsorption during phosphorus removal.The kinetic equation of phosphorus adsorption by FB-OS was qt=10:193t/1+2:574t (R^(2)=0.995).The adsorption rate was mainly controlled by outerfilm diffusion and intraparticle diffusion. | Zhijian Zhou Jie Yan Xinxiang Du Qiulin Xu Zijun Wu Jinlan Yang Xitong Fang Qiuling Zhong Qiaoguang Li | 2023 | Journal of Renewable Materials2023，11，9： | 0 |
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