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| 1 | MSX2 mediates entry of human pluripotent stem cells into mesendoderm by simultaneously suppressing SOX2 and activating NODAL signaling显示文摘发信号的 BMP 怎么集成到并且使动摇人的 pluripotent 干细胞(hPSCs ) 的 pluripotency 电路开始区别进单个细菌层是一个长期的难题。这里,我们报导肌肉片断 homeobox 2 (MSX2 ) , msh 家庭的一个 homeobox 抄写因素, BMP 发信号的直接目标基因和 hPSC 到 mesendoderm 的区别的一个主人调停人。MSX2 的强制表示足够废除 pluripotency 并且导致 hPSCs 的指导 mesendoderm 区别,当 MSX2 弄空损害 mesendoderm 正式就职时。MSX2 是在 hPSCs 的 BMP 小径的直接目标基因,并且能被 Wnt 信号 synergistically 在 mesendoderm 正式就职期间经由 LEF1 激活。而且, MSX2 使动摇通过到 SOX2 倡导者和 SOX2 抄写的压抑的直接绑定的 pluripotency 电路,当 MSX2 通过节的倡导者的直接绑定和激活由 SOX2 的同时的抑制和节的表示的正式就职控制 mesendoderm 系承诺时。有趣地, SOX2 能支持 MSX2 蛋白质的降级,建议在在干细胞命运的控制的二个指定系的因素之间的相互的对抗。一起,我们的调查结果揭示使动摇的关键新机制在 hPSCs 的 pluripotency 和指导系承诺。 | Qingqing Wu Leisheng Zhang Pei Su Xiaohua Lei Xin Liu Hongtao Wang Lisha Lu Yang Bai Tao Xiong Dong Li Zhengmao Zhu Enkui Duan Erlie Jiang Sizhou Feng Mingzhe Hang Yuanfu Xu Fei Wang Jiaxi Zhou | 2015 | Cell Research2015，25，12： | 8 |
| 2 | Single-cell transcriptomic landscape of human blood cells显示文摘High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis.However,an elaborate and comprehensive transcriptome reference of the whole blood system is lacking.Here,we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types,including hematopoietic stem cells,progenitors and mature blood cells derived from 21 healthy donors.With high sequencing depth and coverage,we constructed a single-cell transcriptional atlas of blood cells(ABC) on the basis of both protein-coding genes and long noncoding RNAs(lncRNAs),and showed a high consistence between them.Notably,putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified.While common transcription factor regulatory networks were activated in neutrophils and monocytes,lymphoid cells dramatically changed their regulatory networks during differentiation.Furthermore,we showed a subset of nucleated erythrocytes actively expressing immune signals,suggesting the existence of erythroid precursors with immune functions.Finally,a web portal offering transcriptome browsing and blood cell type prediction has been established.Thus,our work provides a transcriptional map of human blood cells at single-cell resolution,thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis. | Xiaowei Xie Mengyao Liu Yawen Zhang Bingrui Wang Caiying Zhu Chenchen Wang Qing Li Yingying Huo Jiaojiao Guo Changlu Xu Linping Hu Aiming Pang Shihui Ma Una Wang Wenbin Cao Shulian Chen Qiuling Li Sudong Zhang Xueying Zhao Wen Zhou Hongbo Luo Guoguang Zheng Erlie Jiang Sizhou Feng Lixiang Chen Lihong Shi Hui Cheng Sha Hao Ping Zhu Tao Cheng | 2021 | National Science Review2021，8，3： | 3 |
| 3 | Loss of Lkb1 impairs Treg function and stability to aggravate graft-versus-host disease after bone marrow transplantation显示文摘Accumulating evidence suggests that a reduction in the number of Foxp3^(+) regulatory T cells(Tregs)contributes to the pathogenesis of acute graft-versus-host disease(aGVHD),which is a major adverse complication that can occur after allogeneic hematopoietic stem cell transplantation(allo-HSCT).However,the precise features and mechanism underlying the defects in Tregs remain largely unknown.In this study,we demonstrated that Tregs were more dramatically decreased in bone marrow compared with those in peripheral blood from aGVHD patients and that bone marrow Treg defects were negatively associated with hematopoietic reconstitution.Tregs from aGVHD patients exhibited multiple defects,including the instability of Foxp3 expression,especially in response to IL-12,impaired suppressor function,decreased migratory capacity,and increased apoptosis.Transcriptional profiling revealed the downregulation of Lkb1,a previously identified critical regulator of murine Treg identity and metabolism,and murine Lkb1-regulated genes in Tregs from aGVHD patients.Foxp3 expression in human Tregs could be decreased and increased by the knockdown and overexpression of the Lkb1 gene,respectively.Furthermore,a loss-of-function assay in an aGVHD murine model confirmed that Lkb1 deficiency could impair Tregs and aggravate disease severity.These findings reveal that Lkb1 downregulation contributes to multiple defects in Tregs in human aGVHD and highlight the Lkb1-related pathways that could serve as therapeutic targets that may potentially be manipulated to mitigate aGVHD. | Xiuhua Su Qianqian Wang Wei Guo Xiaolei Pei Qing Niu Maolan Liu Yuanyuan Liu Song Chen Sizhou Feng Yi He Donglin Yang Rongli Zhang Qiaoling Ma Weihua Zhai Aiming Pang Jialin Wei Yong Huang Yuechen Luo Mingzhe Han Xiaoming Feng Erlie Jiang | 2020 | Cellular & Molecular Immunology2020，17，5： | 3 |
| 4 | Risk factors associated with hemorrhagic cystitis after allogeneic hematopoietic stem cell transplantation显示文摘Hemorrhagic cystitis(HC)is a common complication of allogeneic hematopoietic stem cell transplantation(HSCT).The incidence is about 7%to 68%,and some patients have to suffer a long period of frequent,urgent,and painful urination,which brings great pain.This study aimed to analyze risk factors of HC and its effect on patient survival.We collected the medical records of 859 patients who underwent HSCT at our hospital between August 2016 and August 2020.Patients with and without HC were matched using propensity score matching at a 1:1 ratio based on sex,age,and diagnosis,and logistic regression analyses were used to identify factors associated with HC.We used Kaplan–Meier curves to analyze the survival rates of patients in the HC and non-HC groups.We also analyzed the relationship between BK viral load and the occurrence of HC using receiver operating characteristic curve(ROC)analysis.After propensity score matching,there were 131 patients each in the HC and non-HC groups.In the HC group,89 patients(67.9%)had mild HC(stage II°)and 43(32.1%)had severe HC(stage III–IV).The median interval between stem cell transplantation and HC development was 31(3–244)days.Univariate analysis indicated that donor age,hematopoietic stem cell source,HLA,acute graft-versus-host disease,busulfan,anti-thymocyte globulin(ATG),total body irradiation,cytomegalovirus(CMV)(urine),and BK polyomavirus(BKV)(urine)were significantly associated with HC.ATG,CMV(urine),and BKV(urine)were independent risk factors for HC based on the multivariate analysis.The Kaplan–Meier survival analysis showed no significant difference between the HC and non-HC groups(P=0.14).The 1-and 2-year survival rates in the HC group were 78.4%and 69.6%,respectively,and the corresponding rates in the non-HC group were 84.4%and 80.7%,respectively.ROC analysis indicated that a urine BKV load of 1×10^(7) copies/mL was able to stratify the risk of HC.In conclusion,when the BKV load is>1×10^(7),we needtobe aware of the potential for the development of HC. | Biao Shen Yueshen Ma Haixiao Zhang Mingyang Wang Jia Liu Jiaxin Cao Wenwen Guo Dan Feng Donglin Yang Rongli Zhang Xin Chen Qiaoling Ma Weihua Zhai Sizhou Feng Mingzhe Han Aiming Pang Erlie Jiang 无 | 2022 | Blood Science2022，4，2： | 2 |
| 5 | Colony-stimulating factor 3 receptor (CSF3R) M696T mutation does not impact on clinical outcomes of a Ph+ acute lymphoblastic leukemia patient显示文摘Colony-stimulating factor 3 receptor(CSF3R)mutations have been identified in a variety of myeloid disorders.Although CSF3R point mutations(eg,T618I)are emerging as key players in chronic neutrophilic leukemia/atypical chronic myelogenous leukemia,the significance of rarer CSF3R mutations is unknown.Here,we report a 32-year-old female who was diagnosed as Philadelphia chromosome-positive acute lymphoblastic leukemia(Ph^(+)ALL)with the CSF3R M696T mutation and was undergone unrelated donor hematopoietic stem cell transplantation.The patient achieved complete remission with chemotherapy in combination with tyrosine kinase inhibitor(TKI)and long-term survival by unrelated donor transplantation.Meanwhile,we performed a series of experiments using murine interleukin 3(IL-3)-dependent Ba/F3 cell line to evaluate the transforming capacity of the CSF3R M696T mutation.We confirmed the presence of a CSF3R M696T germline mutation in this patient which was inherited from her mother.The in vitro experiment results showed that the CSF3R M696T mutation contributes marginally to the tumor transformation of Ba/F3 cells,indicating that CSF3R M696T mutation was neutral in tumor transformation ability.We concluded that TKI is effective in patients with the CSF3R M696T mutation in Ph+ALL and donors with CSF3R M696T mutation might still be selected as the candidate for transplantation. | Xin Chen Bichen Wang Aiming Pang Weiping Yuan Erlie Jiang Yajing Chu Sizhou Feng Mingzhe Han | 2021 | Blood Science2021，3，3： | 0 |
| 6 | Acute myeloid leukemia relapse after allogeneic hematopoietic stem cell transplantation presenting as pericardial effusion显示文摘1.INTRODUCTION.Extramedullary leukemic infiltration can occur as a manifestation of relapse.It is,however,rare with fewer than 10 cases of cardiovascular relapse reported in the literature,and it often presents as myeloid sarcoma.1 We report a case of pericardial effusion presenting as an isolated extramedullary relapse(IEMR)of leukemia after allogeneic hematopoietic stem cell transplantation(allo-HSCT). | Yuyan Shen Jiali Sun Donglin Yang Sizhou Feng | 2023 | Blood Science2023，5，4： | 0 |
| 7 | Risk factors for CMV infection within 100 days posttransplantation in patients with acute leukemia显示文摘Objective:To investigate the risk factors for cytomegalovirus(CMV)infection within 100 days and the relationship between early CMV infection and 1-year relapse for patients with acute leukemia following allogeneic hematopoietic stem cell transplantation(allo-HSCT).Methods:Three hundred fifty-nine patients with acute leukemia who received allo-HSCT at our center between January 2015 and January 2020 were retrospectively reviewed.Results:Of 359 patients,48.19%(173)patients experienced CMV infection within 100 days posttransplantation.In univariate and multivariate logistic analysis,haploidentical-related donor(HRD)(P<0.001;odds ratio[OR],5.542;95%confidence interval[CI],3.186–9.639),and ratio of CD3^(+)CD8^(+)cells in lymphocytes<14.825%(P<0.001;OR,3.005;95%CI,1.712–5.275)were identified as 2 independent risk factors.One-year relapse rate(RR)between the CMV infection group and the non-CMV infection group was not statistically significant(18.5%vs 19.9%,P=0.688).When we divided the total cohort into AML,ALL,and MAL subgroups,there were no significant differences as well(P=0.138;P=0.588;P=0.117;respectively).Conclusion:In conclusion,donor type(HRD)and the insufficient recovery of CD3^(+)CD8^(+)cells were independent risk factors for CMV infection within 100 days posttransplantation in patients with acute leukemia.CMV infection within 100 days did not influence the incidence of relapse in 1 year for patients with acute leukemia. | Juan Chen Aiming Pang Yuanqi Zhao Li Liu Runzhi Ma Jialin Wei Xin Chen Yi He Donglin Yang Rongli Zhang Weihua Zhai Qiaoling Ma Erlie Jiang Mingzhe Han Jiaxi Zhou Sizhou Feng | 2022 | Blood Science2022，4，3： | 0 |
| 8 | Intravenous-oral itraconazole versus oral posaconazole in preventing invasive fungal diseases for acute leukemia patients显示文摘Invasive fungal diseases(IFDs)are major and lethal infectious complications for patients with neutropenia after chemotherapy.Prophylaxis with intravenous and oral suspended itraconazole(200 mg Q12h intravenously×2 days followed by 5 mg/kg·d orally in twice)or oral suspension of posaconazole(200 mg Q8h)was administered for preventing IFDs.The only 2 episodes of proven IFDs were not included after propensity-score matching(PSM),while the incidence of possible IFDs was 8.2%(9/110)in itraconazole group and 1.8%(2/110)in posaconazole group,respectively(P=.030).In clinical failure analysis,the failure rate of posaconazole group was lower as compared to the itraconazole group(2.7%vs 10.9%,P=.016).Both intravenous-oral itraconazole and posaconazole suspension are effective in preventing IFDs,while posaconazole suspension seems more tolerable. | Li Liua Xiaolei Peia Runzhi Maa Yi Hea Rongli Zhanga Jialin Weia Qiaoling Maa Weihua Zhaia Aiming Pang Erlie Jiang Mingzhe Han Donglin Yang Sizhou Feng | 2023 | Blood Science2023，5，2： | 0 |
| 9 | Hole-Transporting Low-Dimensional Perovskite for EnhancingPhotovoltaic Performance显示文摘Halide perovskites with low-dimensionalities(2D or quasi-2D)have demonstrated outstanding stabilities compared to their 3D counterparts.Nevertheless,poor charge-transporting abilities of organic components in 2D perovskites lead to relatively low power conversion efficiency(PCE)and thus limit their applications in photovoltaics.Here,we report a novel hole-transporting low-dimensional(HT2D)perovskite,which can form a hole-transporting channel on the top surface of 3D perovskite due to self-assembly effects of metal halide frameworks.This HT2D perovskite can significantly reduce interface trap densities and enhance hole-extracting abilities of a heterojunction region between the 3D perovskite and hole-transporting layer.Furthermore,the posttreatment by HT2D can also reduce the crystal defects of perovskite and improve film morphology.As a result,perovskite solar cells(PSCs)can effectively suppress nonradiative recombination,leading to an increasement on photovoltage to>1.20 V and thus achieving>20%power conversion efficiency and>500 h continuous illumination stability.This work provides a pathway to overcome charge-transporting limitations in low-dimensional perovskites and delivers significant enhancements on performance of PSCs. | Fangfang Wang Qing Chang Yikai Yun Sizhou Liu You Liu Jungan Wang Yinyu Fang Zhengchun Cheng Shanglei Feng Lifeng Yang Yingguo Yang Wei Huang Tianshi Qin | 2021 | Research2021，，1： | 0 |
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