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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Effects of culm carbohydrate partitioning on basal stem strength in a high-yielding rice population显示文摘Rice culm carbohydrate transport can simultaneously affect grain filling and stem lodging resistance by regulating non-structural carbohydrate(NSC) and structural carbohydrate(SC) contents. However, the relationship between carbohydrate transposition and culm strength is not well documented. Accordingly, a high-yielding hybrid rice cultivar(Y Liangyou 2) was tested under different N fertilization regimes at two locations, Taoyuan(a special high-yield eco-site), Yunnan province and Danyang(a representative eco-site of the middle and lower Yangtze), Jiangsu province, China. Significantly higher grain yield and basal stem strength were found at Taoyuan than Danyang under all N rates throughout the two-year experiment. At heading stage, soluble sugars, starch, cellulose and lignin contents of the basal culm at Taoyuan were significantly 132.0%, 73.7%, 1.2%, and 62.7% higher than those at Danyang, respectively. At 20 days after heading, soluble sugars and starch content at Taoyuan decreased significantly compared to Danyang, but lignin content remained higher. Culm carbohydrate transport to kernels at Taoyuan was significantly greater than that at Danyang, and the proportion of soluble sugars and starch was correspondingly 62.9%lower. However, the proportion of lignin and cellulose was 22.7% higher at Taoyuan than that at Danyang. Soluble sugars and starch partitioning were significantly reduced under an increased nitrogen application rate, but SC partitioning was little affected. There were significant positive correlations between basal culm bending stress and dry weight and cellulose and lignin proportions at both locations under all N rates, suggesting that the higher SC proportion at 20 days after heading was primarily responsible for culm strength.These results suggest that high-yielding rice populations with greater culm strength require both moderate NSC transport and greater SC accumulation. | Jun Zhang Ganghua Li Qingyu Huang Zhenghui Liu Chengqiang Ding She Tang Lin Chen Shaohua Wang Yanfeng Ding Weijian Zhang | 2017 | The Crop Journal2017，5，6： | 13 |
| 3 | Plasma metabolomic and lipidomic alterations associated with COVID-19显示文摘The pandemic of the coronavirus disease 2019(COVID-19)has become a global public health crisis.The symptoms of COVID-19 range from mild to severe,but the physiological changes associated with COVID-19 are barely understood.In this study,we performed targeted metabolomic and lipidomic analyses of plasma from a cohort of patients with COVID-19 who had experienced different symptoms.We found that metabolite and lipid alterations exhibit apparent correlation with the course of disease in these patients,indicating that the development of COVID-19 affected their whole-body metabolism.In particular,malic acid of the TCA cycle and carbamoyl phosphate of the urea cycle result in altered energy metabolism and hepatic dysfunction,respectively.It should be noted that carbamoyl phosphate is profoundly down-regulated in patients who died compared with patients with mild symptoms.And,more importantly,guanosine monophosphate(GMP),which is mediated not only by GMP synthase but also by CD39 and CD73,is significantly changed between healthy subjects and patients with COVID-19,as well as between the mild and fatal cases.In addition,dyslipidemia was observed in patients with COVID-19.Overall,the disturbed metabolic patterns have been found to align with the progress and severity of COVID-19.This work provides valuable knowledge about plasma biomarkers associated with COVID-19 and potential therapeutic targets,as well as an important resource for further studies of the pathogenesis of COVID-19. | Di Wu Ting Shu Xiaobo Yang Jian-Xin Song Mingliang Zhang Chengye Yao Wen Liu Muhan Huang Yuan Yu Qingyu Yang Tingju Zhu Jiqian Xu Jingfang Mu Yaxin Wang Hong Wang Tang Tang Yujie Ren Yongran Wu Shu-Hai Lin Yang Qiu Ding-Yu Zhang You Shang Xi Zhou | 2020 | National Science Review2020，7，7： | 9 |
| 4 | High thermal conductivity of suspended few-layer hexagonal boron nitride sheets显示文摘推迟的很少层的热传导六角形的硼氮化物(h-BN ) 表试验性地用一个 noncontact micro-Raman 光谱学方法被调查。为单层(1L ) 的一阶的温度系数, bilayer (2L ) 并且九层(9L ) h-BN 表被测量是(当时, electrospinning 准备的 3.41 xide nanofibers 被期望减少了热电导率与体积样品相比。自从它包含复杂样品准备方法,热电导率正在质问的 nanofibers 的大小。在这个工作,我们在场对单个 nanofiber 的热电导率的大小合适的一个新奇方法。一 microelectro 机械(MEMS ) 设备被设计了并且制作了在单个 nanofiber 上执行热传导性大小。一个特殊 Si 模板被设计收集并且转单个 nanofibers 到 MEMS 设备名上吗? | Haiqing Zhou Jixin Zhu Zheng Liu Zheng Yan Xiujun Fan Jian Lin Gunuk Wang Qingyu Yan Ting Yu Pulickel M. Ajayan James M. Tour | 2014 | Nano Research2014，7，8： | 9 |
| 5 | Optical properties of NaY(MoO4)2:Eu3+ nanophosphors prepared by molten salt method显示文摘We synthesized NaY(MoO4)2:Eu3+phosphors of different doping concentrations by a molten salt method.This facile way possesses advantages such as simple process,lower calcination temperature(350℃)and small particle size(70 nm).The crystal system is tetragonal phase and crystal lattice is body centered.The photo luminescence measurements including emission spectra,excitation spectra and fluorescence decay curves were carried out,elucidating that NaY(MoO4)2:Eu3+can be effectively excited by near UV and blue light.Moreover,it can be concluded that Eu3+energy transfer type is exchange interaction.Huang-Rhys factor and the critical energy transfer distance(Rc)were calculated to be 0.043 and 0.995 nm,respectively.Auzel’s model was used to obtain the intrinsic radiative transition lifetime of5 D0 level(τ0=0.923 ms).Furthermore,a calculation method was used to calculate refractive index n of nontransparent NaY(MoO4)2:1 mol%Eu3+phosphor,and n was obtained to be 1.86. | Lin Bai Qingyu Meng Mingxue Huo Yu Sui | 2019 | Journal of Rare Earths2019，37，12： | 8 |
| 6 | Influences of accumulated mileage and technological changes on emissions of regulated pollutants from gasoline passenger vehicles显示文摘In this study, the influences of accumulated mileage(deterioration) and technological changes(emission standards) on emission factors(EFs) of regulated pollutants(CO, HC, and NO_x) from gasoline passenger vehicles were investigated based on Inspection and Maintenance(I/M) data using the chassis dynamometer method. The accumulated mileage of passenger vehicles was significantly linearly correlated with vehicle age. For most cases,the average EFs of CO, HC and NO_x were significantly linearly correlated with accumulated mileage, indicating that emission deterioration had a significant impact on pollutant EFs.Implemented emission standards markedly influenced the EFs of regulated pollutants, and EFs markedly decreased with progressing emission standards. The present study also compared EFs of regulated pollutants between this study and the International vehicle emission(IVE) model, and marked differences in EFs were seen with variations in emission standards, vehicle types and accumulated mileage; NO_x EFs in this study were higher than in the IVE model. The results provide new insight into estimating regulated pollutant emissions using the IVE model. | Qingyu Zhang Juwang Fan Weidong Yang Fang Ying Zhen Bao Yi Sheng Cheng Lin Xuan Chen | 2018 | Journal of Environmental Sciences2018，30，9： | 5 |
| 7 | Direct Introduction of EthoxycarbonyldifluoromethyI-Group to Heteroarenes with Ethyl Bromodifluoroacetate via Visible-Light Photocatalysis显示文摘为到经由可见光的光催化的 heteroarenes 的 ethoxycarbonyldifluoromethyl 组的直接介绍的一条温和、万用的途径被开发了。新 photoredox 协议启用了在温和条件下面包含大量普通的功能的组的 heteroarenes 的 difluoromethylenation。 | Qingyu Lin Lingling Chu Feng-Ling Qing | 2013 | Chinese Journal of Chemistry2013，31，7： | 4 |
| 8 | Angular-based spatially resolved laser-induced breakdown spectroscopy: a new technique for the effective enhancement of signals without an external time delay system显示文摘In this study, we developed a novel angularbased spatially resolved laser-induced breakdown spectroscopy(ABSR-LIBS) technique. This new method allows the spatially resolved spectroscopic properties of laserinduced plasma to be investigated using different angles and polar distances with a spatially adjustable fiber optics probe. The experimental evaluations suggested that the intensity of the Al signal distributions in the ‘‘side-view''depended greatly on the angle of the collection probe. The location of a collection probe at 50° would facilitate efficient spectral collection and minimize the disturbance of the plasma plume. The spectral signals in the plasma emissions had different distribution at various polar distances using the same collection angle, and the highest signal intensity was observed between 15 and 35 mm polar distances. This ABSR-LIBS technique improved both the LIBS signal and the maximum emission intensity of Al atoms by several times at the best spatial position in our experimental conditions. Furthermore, we successfully obtained well-resolved line emission spectroscopy signals without any external time delay using a nongated spectrometer based on the ABSR-LIBS system. We consider that the ABSR-LIBS technique has great potential for obtaining new insights with laser-induced plasma instruments and it could facilitate the development of a low-cost instrument. | Qingyu Lin Xu Wang Guanghui Niu Hongjun Lai Xiaoqin Zhu Kunping Liu Tao Xu Yixiang Duan | 2014 | Chinese Science Bulletin2014，59，27： | 3 |
| 9 | Standard pancreatoduodenectomy versus extended pancreatoduodenectomy with modified retroperitoneal nerve resection in patients with pancreatic head cancer:a multicenter randomized controlled trial显示文摘Background:The extent of pancreatoduodenectomy for pancreatic head cancer remains controversial,and more high-level clinical evidence is needed.This study aimed to evaluate the outcome of extended pancreatoduodenectomy(EPD)with retroperitoneal nerve resection in pancreatic head cancer.Methods:This multicenter randomized trial was performed at 6 Chinese highvolume hospitals that enrolled patients between October 3,2012,and September 21,2017.Four hundred patients with stage I or II pancreatic head cancer and without specific pancreatic cancer treatments(preoperative chemotherapy or chemoradiation)within three months were randomly assigned to undergo standard pancreatoduodenectomy(SPD)or EPD,with the latter followed by dissection of additional lymph nodes(LNs),nerves and soft tissues 270◦on the right side surrounding the superior mesenteric artery and celiac axis.The primary endpoint was overall survival(OS)by intention-to-treat(ITT).The secondary endpoints were disease-free survival(DFS),mortality,morbidity,and postoperative pain intensity.Results:TheR1 ratewas slightly lower with EPD(8.46%)thanwith SPD(12.56%).The morbidity and mortality rates were similar between the two groups.The median OS was similar in the EPD and SPD groups by ITT in the whole study cohort(23.0 vs.20.2 months,P=0.100),while the median DFS was superior in the EPD group(16.1 vs.13.2 months,P=0.031).Patients with preoperative CA19–9<200.0 U/mL had significantly improved OS and DFS with EPD(EPD vs.SPD,30.8 vs.20.9 months,P=0.009;23.4 vs.13.5 months,P<0.001).The EPD group exhibited significantly lower locoregional(16.48%vs.35.20%,P<0.001)andmesenteric LNrecurrence rates(3.98%vs.10.06%,P=0.022).The EPD group exhibited less back pain 6 months postoperation than the SPD group.Conclusions:EPD for pancreatic head cancer did not significantly improve OS,but patients with EPD treatment had significantly improved DFS.In the subgroup analysis,improvements in bothOS and DFS in the EPD armwere observed in patients with preoperative CA19–9<200.0 U/mL.EPD could be used as an effective surgical procedure for patients with pancreatic head cancer,especially those with preoperative CA19–9<200.0 U/mL. | Qing Lin Shangyou Zheng Xianjun Yu Meifu Chen Yu Zhou Quanbo Zhou Chonghui Hu Jing Gu Zhongdong Xu LinWang Yimin Liu Qingyu Liu MinWang Guolin Li He Cheng Dongkai Zhou Guodong Liu Zhiqiang Fu Yu Long Yixiong Li Weilin Wang Renyi Qin Zhihua Li Rufu Chen | 2023 | Cancer Communications2023，43，2： | 3 |
| 10 | Research on preparation of heavy calcium carbonate functional filler with mechanical chemical methods 显示文摘 | Tang Quan Lin Qingyu Zhang Zhi | 2015 | Advanced Manufacturing Technology and Materials2015，1089，： | 1 |
| 11 | A Multifunctional Sampling Chamber for Laser-Induced Breakdown Spectroscopy for On-Site Elemental Analysis显示文摘 | Wang Shuai Xu Mingjun Lin Qingyu | 2015 | Instrumentation Science and Technology2015，43，4： | 1 |
| 12 | Kinetic parameters and mechanisms of the batch biosorption of Cr (Ⅵ) and Cr (Ⅲ) onto Leersia hexandra Swartz biomass显示文摘 | Jianping lJ a Qingyu Lin Xuehong Zhang | 2009 | Journal of Colloid and Interface Science2009，333，： | 1 |
| 13 | Application of zeolitic material synthesized from thermally treated sediment to the removal of trivalent chromium from wastewater显示文摘 | Qingyu Guan Deyi Wu Yan Lin | 2009 | Journal of Hazardous Materials2009，167，13： | 1 |
| 14 | Kinetic parameters and mechanisms of the batch biosorption of Cr(VI) and Cr(III) onto Leersia hexandra Swartz biomass显示文摘 | Jianping Li Qingyu Lin Xuehong Zhang Yan Yan | 2009 | Journal of Colloid And Interface Science2009，，1： | 1 |
| 15 | Adsorption of lead and mercury by rice husk ash显示文摘 | Qingge Feng Qingyu Lin Fuzhong Gong | 2004 | Journal of Colloid and Interface Science2004，，278： | 1 |
| 16 | Development and evaluation of a method for hexavalent chromium in ambient air using IC-ICP-MS显示文摘 | Qingyu Meng Zhihua (Tina) Fan Brian Buckley Lin Lin Lihui Huang Chang-Ho Yu Robert Stiles Linda Bonanno | 2011 | Atmospheric Environment2011，，12： | 1 |
| 17 | Adsorption of lead and mercury by rice husk ash显示文摘 | Qingge Feng Qingyu Lin Fuzhong Gong Shuichi Sugita Masami Shoya | 2004 | Journal of Colloid And Interface Science2004，，1： | 1 |
| 18 | Genome assembly and transcriptome analysis provide insights into the antischistosome mechanism of Microtus fortis显示文摘Microtus fortis is the only mammalian host that exhibits intrinsic resistance against Schistosoma japonicum infection.However,the underlying molecular mechanisms of this resistance are not yet known.Here,we perform the first de novo genome assembly of M.fortis,comprehensive gene annotation analysis,and evolution analysis.Furthermore,we compare the recovery rate of schistosomes,pathological changes,and liver transcriptomes between M.fortis and mice at different time points after infection.We observe that the time and type of immune response in M.fortis are different from those in mice.M.fortis activates immune and inflammatory responses on the 10th day post infection,such as leukocyte extravasation,antibody activation,Fc-gamma receptor-mediated phagocytosis,and the interferon signaling cascade,which play important roles in preventing the development of schistosomes.In contrast,an intense immune response occurrs in mice at the late stages of infection and could not eliminate schistosomes.Infected mice suffer severe pathological injury and continuous decreases in cell cycle,lipid metabolism,and other functions.Our findings offer new insights into the intrinsic resistance mechanism of M.fortis against schistosome infection.The genome sequence also provides the basis for future studies of other important traits in M.fortis. | Hong Li Zhen Wang Shumei Chai Xiong Bai Guohui Ding Yuanyuan Li Junyi Li Qingyu Xiao Benpeng Miao Weili Lin Jie Feng Mingyue Huang Cheng Gao Bin Li Wei Hu Jiaojiao Lin Zhiqiang Fu Jianyun Xie Yixue Li | 2020 | Journal of Genetics and Genomics2020，47，12： | 1 |
| 19 | Thermoelectric Transport Properties of TmAg_(x)Cu_(1-x)Te_(2) solid solutions显示文摘Intrinsic partial occupation of Cu induces strong phonon scattering for an extremely low lattice thermal conductivity in TmCuTe_(2),which leads this material to be a promising candidate for thermoelectric applications.This work focus on the electronic and phononic transport properties of TmCuTe_(2) with Agalloying on the Cu site.Such a Ag/Cu substitution is found to enable a decrease in Hall carrier concentration from 141019 to 81019 cm^(3) for an evaluation of the charge transport based on a single parabolic band(SPB)model with acoustic scattering.In addition,Ag-substitution simultaneously introduces Cu/Ag point defects for extra phonon scattering,leading the lattice thermal conductivity to be as low as~0.25 W/m-K in a broad temperature.The optimization in carrier concentration and the reduction in lattice thermal conductivity contribute to a~40%improvement in average thermoelectric figure of merit(zT).The determination of band parameters enables a guidance for further advancements in this promising thermoelectric material. | Qingyu Bai Xinyue Zhang Bing Shan Xuemin Shi Cheng Sun Siqi Lin Wen Li Yanzhong Pei | 2021 | Journal of Materiomics2021，7，4： | 0 |
| 20 | PLX8394,a RAF inhibitor,inhibits enterovirus 71 replication by blocking RAF/MEK/ERK signaling显示文摘Enterovirus 71(EV71)poses a serious threat to human health,with scattered outbreaks worldwide.There are several vaccines against a few EV71 strains but no efficient drug for the treatment of EV71 infection.Therefore,it is urgent and of significance to develop anti-EV71 drugs.Here,we found that PLX8394,a RAF inhibitor,possesses high antiviral activity against EV71 in vitro,being superior to the traditional clinical drug ribavirin.Moreover,PLX8394 exhibits broad-spectrum antiviral activity against enteroviruses.Notably,in a suckling mouse model,PLX8394 provided a 70%protection rate for EV71-infected mice,reduced the viral load in liver and heart tissues,and relieved the inflammatory response.A mechanistic study showed that PLX8394 inhibited EV71 by suppressing the RAF/MEK/ERK signaling pathway.Thus,PLX8394 lays a foundation for the development of new drugs against EV71. | Chengyuan Wu Guangyan Zhu Fang Qiu Fuli Ren Binbin Lin Dingyu Zhang Qingyu Yang Chaolin Huang | 2023 | Virologica Sinica2023，38，2： | 0 |
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