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| 1 | H7N9 virulent mutants detected in chickens in China pose an increased threat to humans显示文摘 | Jianzhong Shi Guohua Deng Huihui Kong Chunyang Gu Shujie Ma Xin Yin Xianying Zeng Pengfei Cui Yan Chen Huanliang Yang Xiaopeng Wan Xiurong Wang Liling Liu Pucheng Chen Yongping Jiang Jinxiong Liu Yuntao Guan Yasuo Suzuki Mei Li Zhiyuan Qu Lizheng Guan Jinkai Zang Wenli Gu Shuyu Han Yangming Song Yuzhen Hu Zeng Wang Linlin Gu Wenyu Yang Libin Liang Hongmei Bao Guobin Tian Yanbing Li Chuanling Qiao Li Jiang Chengjun Li Zhigao Bu Hualan Chen | 2017 | Cell Research2017，27，12： | 70 |
| 2 | Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China显示文摘The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza. | Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen | 2022 | Science China(Life Sciences)2022，65，4： | 12 |
| 3 | Physiological responses of osteoblasts to cyclic stretching and the change of intracellular calcium concentration显示文摘The development of bone tissues is regulated by mechanical stimulation. Cyclic stretching was applied to the osteoblasts that were delivered from rat calvarie. The results showed that stretching at 500 me increased the cell prolifera-tion while loading at 1000 me and 1500 me inhabited cell growth. Loading also increased the adhesive force between cells and substrate as well as spreading areas of osteobalsts. Furthermore, the fluorescence probe Fluo-3/AM was used to investigate the effect of stretching stimulation on the intra-cellular calcium concentration of osteoblasts. The intracellu-lar calcium concentration of osteoblasts that were stretched at 500 me for 5 min was 92.9% higher than the control. After being treated with the panax ontoginseng saponins, the stretched osteoblasts still expressed 28.6% higher intracellu-lar calcium concentration than that of the control, which proved that both the influx of extracellular calcium and the release of intracellular calcium store were involved in the increase of intracellular calcium concentration when os-teoblasts responded to the cyclic stretching. And the influx of extracellular calcium through transmembrance channel played a main role. | TANG Liling, WANG Yuanliang, GU Li, CAI Shaoxi & SU Aihua Bio-engineering College, Chongqing University, Chongqing 400044, China Correspondence should be addressed to Tang Liling | 2003 | Chinese Science Bulletin2003，48，4： | 7 |
| 4 | A Method for Alpine Wetland Delineation and Features of Border: Zoigê Plateau, China显示文摘Accurate wetland delineation is the basis of wetland definition and mapping, and is of great importance for wetland management and research. The Zoigê Plateau on the Qinghai-Tibet Plateau was used as a research site for research on alpine wetland delineation. Several studies have analyzed the spatiotemporal pattern and dynamics of these alpine wetlands, but none have addressed the issues of wetland boundaries. The objective of this work was to discriminate the upper boundaries of alpine wetlands by coupling ecological methods and satellite observations. The combination of Landsat 8 images and supervised classification was an effective method for rapid identification of alpine wetlands in the Zoigê Plateau. Wet meadow was relatively stable compared with hydric soils and wetland hydrology and could be used as a primary indicator for discriminating the upper boundaries of alpine wetlands. A slope of less than 4.5° could be used as the threshold value for wetland delineation. The normalized difference vegetation index(NDVI) in 434 field sites showed that a threshold value of 0.3 could distinguish grasslands from emergent marsh and wet meadow in September. The median normalized difference water index(NDWI) of emergent marsh remained more stable than that of wet meadow and grasslands during the period from September until July of the following year. The index of mean density in wet meadow zones was higher than the emergent and upland zones. Over twice the number of species occurred in the wet meadow zone compared with the emergent zone, and close to the value of upland zone. Alpine wetlands in the three reserves in 2014 covered 1175.19 km2 with a classification accuracy of 75.6%. The combination of ecological methods and remote sensing technology will play an important role in wetland delineation at medium and small scales. The correct differentiation between wet meadow and grasslands is the key to improving the accuracy of future wetland delineation. | ZHENG Yaomin NIU Zhenguo GONG Peng LI Mengna HU Lile WANG Lei YANG Yuxiang GU Hai-jun MU Jinrong DOU Gejia XUE Hui WANG Lin LI Hua DOU Gejie DANG Zhicairang | 2017 | Chinese Geographical Science2017，27，5： | 2 |
| 5 | H7N9 Influenza Viruses Are Transmissible in Ferrets by Respiratory Droplet显示文摘 | Qianyi Zhang Jianzhong Shi Guohua Deng Jing Guo Xianying Zeng Xijun He Huihui Kong Chunyang Gu Xuyong Li Jinxiong Liu Guojun Wang Yan Chen Liling Liu Libin Liang Yuanyuan Li Jun Fan Jinliang Wang Wenhui Li Lizheng Guan Qimeng Li Huanliang Yang Pucheng Che | 2013 | Science2013，，6144： | 1 |
| 6 | Glycosylation and an amino acid insertion in the head of hemagglutinin independently affect the antigenic properties of H5N1 avian influenza viruses显示文摘Antigenic drift forces us to frequently update influenza vaccines; however, the genetic basis for antigenic variation remains largely unknown. In this study, we used clade 7.2 H5 viruses as models to explore the molecular determinants of influenza virus antigenic variation. We generated eight monoclonal antibodies(MAbs) targeted to the hemagglutinin(HA) protein of the index virus A/chicken/Shanxi/2/2006 and found that two representative antigenically drifted clade 7.2 viruses did not react with six of the eight MAbs. The E131 N mutation and insertion of leucine at position 134 in the HA protein of the antigenically drifted strains eliminated the reactivity of the virus with the MAbs. We also found that the amino acid N131 in the H5 HA protein is glycosylated. Our results provide experimental evidence that glycosylation and an amino acid insertion or deletion in HA influence antigenic variation. | Chunyang Gu Xianying Zeng Yangming Song Yanbing Li Liling Liu Yoshihiro Kawaoka Dongming Zhao Hualan Chen | 2019 | Science China(Life Sciences)2019，62，1： | 1 |
| 7 | Determination of nifeviroc, a novel CCR5 antagonist: Application to a pharmacokinetic study显示文摘 | Wei Wu Dong Xie Zeneng Cheng Zhi Liu Liling Ran Zhenkun Gu Peng Yu | 2011 | Journal of Pharmaceutical and Biomedical Analysis2011，，3： | 1 |
| 8 | H7N9 Influenza Viruses Are Transmissible in Ferrets by Respiratory Droplet显示文摘 | Qianyi Zhang Jianzhong Shi Guohua Deng Jing Guo Xianying Zeng Xijun He Huihui Kong Chunyang Gu Xuyong Li Jinxiong Liu Guojun Wang Yan Chen Liling Liu Libin Liang Yuanyuan Li Jun Fan Jinliang Wang Wenhui Li Lizheng Guan Qimeng Li Huanliang Yang Pucheng Che | 2013 | Science2013，，6144： | 1 |
| 9 | 2017年中国出现的H7N9高致病性病毒的快速进化显示文摘文章简介为了防控H7N9病毒,中国2017年9月开始对鸡进行了H5/H7二价禽流感灭活疫苗。为了解H7N9病毒在中国家禽中的分布情况,研究团队进行了系统的禽流感病毒监测。2017年2月至2017年5月之间在379个活禽市场和665个家禽养殖场共采集30201份样品(其中活禽市场采集12504份. | Jianzhong Shi Guohua Deng Shujie Ma Xianying Zeng Xin Yin Mei Li Bo Zhang Pengfei Cui Yan Chen Huanliang Yang Xiaopeng Wan Liling Liu Pucheng Chen Yongping Jiang Yuntao Guan Jinxiong Liu Wenli Gu Shuyu Han Yangming Song Libin Liang Zhiyuan Qu Yujie Hou Xiurong Wang Hongmei Bao Guobin Tian Yanbing Li Li Jiang Chengjun Li 陈化兰 | 2019 | 科学新闻2019，0，2： | 1 |
| 10 | Autophagy and gap junctional intercellular communication inhibition are involved in cadmium-induced apoptosis in rat liver cells显示文摘 | Hui Zou Liling Zhuo Tao Han Di Hu Xiaokang Yang Yi Wang Yan Yuan Jianhong Gu Jianchun Bian Xuezhong Liu Zongping Liu | 2015 | Biochemical and Biophysical Research Communicatio2015，，4： | 1 |
| 11 | Preparation and characterization of methacrylated gelatin/bacterial cellulose composite hydrogels for cartilage tissue engineering显示文摘Methacrylated gelatin(GelMA)/bacterial cellulose(BC)composite hydrogels have been successfully prepared by immersing BC particles in GelMA solution followed by photo-crosslinking.The morphology of GelMA/BC hydrogel was examined by scanning electron microscopy and compared with pure GelMA.The hydrogels had very well interconnected porous network structure,and the pore size decreased from 200 to 10 mm with the increase of BC content.The composite hydrogels were also characterized by swelling experiment,X-ray diffraction,thermogravimetric analysis,rheology experiment and compressive test.The composite hydrogels showed significantly improved mechanical properties compared with pure GelMA.In addition,the biocompatility of composite hydrogels were preliminarily evaluated using human articular chondrocytes.The cells encapsulated within the composite hydrogels for 7 days proliferated and maintained the chondrocytic phenotype.Thus,the GelMA/BC composite hydrogels might be useful for cartilage tissue engineering. | Liling Gu Tao Li Xiongbo Song Xianteng Yang Senlei Li Long Chen Pingju Liu Xiaoyuan Gong Cheng Chen Li Sun | 2020 | Regenerative Biomaterials2020，7，2： | 0 |
| 12 | Thermosensitive injectable hydrogel loaded with hypoxia-induced exosomes maintains chondrocyte phenotype through NDRG3-mediated hypoxic response显示文摘Current clinical treatments cannot effectively delay the progression of osteoarthritis(OA).Consequently,joint replacement surgery is required for late-stage OA when patients cannot tolerate pain and joint dysfunction.Therefore,the prevention of OA progression in the early and middle stages is an urgent clinical problem.In a previous study,we demonstrated that NDRG3-mediated hypoxic response might be closely related to the development and progression of OA.In this study,an injectable thermosensitive hydrogel was established by cross-linking Pluronic F-127 and hyaluronic acid(HA)for the sustained release of hypoxia-induced exosomes(HExos)derived from adipose-derived mesenchymal stem cells.We demonstrated that for OA at the early and middle stages,the HExos-loaded HP hydrogel could maintain the chondrocyte phenotype by enhancing chondrocyte autophagy,reducing chondrocyte apoptosis,and promoting chondrocyte activity and proliferation through the NDRG3-mediated hypoxic response.This novel composite hydrogel,which could activate the NDRG3-mediated hypoxic response,may provide new ideas and a theoretical basis for the treatment of early-and mid-stage OA. | Xiongbo Song Liling Gu Qiming Yang Jiarui Wu Junrong Chen Xiaobin Tian Li Sun Long Chen | 2023 | Chinese Chemical Letters2023，34，8： | 0 |
| 13 | The Effect of Cyclic Stretching on Matrix Production, Mineralization and Differentiation of Osteoblasts显示文摘A four-point bending apparatus is used to investigate the effects of stretching on collagen synthesis, mineralization and differentiation of osteoblasts. Cells are stretched at 1500 με for 24 hours. The responses of osteoblasts to mechanical signal of physiological stretching are evaluated from three aspects: collagen production, extracellular inorganic calcium secretion and ALP activity. The results show that osteoblasts decrease the collagen synthesis, calcium secretion and ALP activity compared to the control cells (65.82%,73.51%,48.10% respectively), confirming that cyclic stretching at 1500 με inhabits the physiological activity of osteoblasts. | 秦建 TANG LILING Wang Yuanliang Gu Li | 2003 | High Technology Letters2003，9，3： | 0 |
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