|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 成骨细胞中NO及c-fos对流体剪切力的响应显示文摘目的:研究NO及c-fos对不同流体剪切力作用时间的生理响应。方法:分离新生SD大鼠颅盖骨中的成骨细胞并分成三组,分别使用含10%FBS、0.3 mm o l/L L-NMMA和0.1 mm o l/LSNP的DM EM培养液预处理24 h,加载大小为1.2 Pa的剪切力。每组在加载剪切力0 m in、10 m in、15 m in、30 m in、60 m in后分别测试c-fosmRNA、NOS的表达。结果:c-fosmRNA表达在水平剪切力加载15 m in时明显增高(P<0.05),在使用L-NMMA后明显降低(P<0.05),而使用SNP后明显升高(P<0.05)。结论:c-fos和NO参与了力学刺激引发细胞响应的过程,特别是在外界信号刺激引起的信息传递级联反应中起重要的偶联作用。 | 朱赴东 赵士芳 童晓艳 方进华 | 2006 | 浙江大学学报(医学版)2006，35，5： | 5 |
| 2 | A model of calcium signaling and degranulation dynamics induced by laser irradiation in mast cells显示文摘Recent experiments show that calcium signaling and degranulation dynamics induced by low power laser irradiation in mast cells must rely on extracellular Ca2+ influx. An analytical expression of Ca2+ flux through TRPV4 cation channel in response to interaction of laser photon energy and extracellular Ca2+ is deduced, and a model characterizing dynamics of calcium signaling and degranulation activated by laser irradiation in mast cells is established. The model indicates that the characteristics of calcium signaling and degranulation dynamics are determined by interaction between laser photon energy and Ca2+ influx. Extracellular Ca2+ concentration is so high that even small photon energy can activate mast cells, thus avoiding the possible injury caused by laser irradiation with shorter wavelengths. The model predicts that there exists a narrow parameter domain of photon energy and extracellular Ca2+ concen-tration of which results in cytosolic Ca2+ limit cycle oscillations, and shows that PKC activity is in direct proportion to the frequency of Ca2+ oscillations. With the model it is found that sustained and stable maximum plateau of cytosolic Ca2+ concentration can get optimal degranulation rate. Furthermore, the idea of introducing the realistic physical energy into model is applicable to modeling other physical signal transduction systems. | SHI XiaoMin ZHENG YuFan LIU ZengRong YANG WenZhong | 2008 | Chinese Science Bulletin2008，53，15： | 3 |
| 3 | 成骨细胞响应机械刺激的力化学转导机理显示文摘应力环境可调节骨组织的生长、吸收和重建。在细胞水平上,机械刺激可以影响成骨细胞的生理活性,如增殖、碱性磷酸酶活性、骨钙素合成等。力转导是将生物物理力转化为细胞的生化响应的过程,它是许多生理功能的基础,早期响应基因(c- fos,c- jun) ,第二信使系统(Ca2 + ,NO,c AMP) 。 | 王远亮 唐丽灵 王建华 蔡绍皙 | 2005 | 生物医学工程学杂志2005，22，2： | 3 |
| 4 | 大鼠成骨细胞对动态拉伸刺激的生理响应显示文摘应用体外周期性机械拉伸装置研究机械拉伸对大鼠成骨细胞增殖及分化的影响。测试了在应变15%,频率20次/min的拉伸刺激下不同加载时间对成骨细胞增殖及分化的影响。发现在受载12h时,细胞相对增殖指数最大,随受载时间的增加而逐渐趋向于1,受载36h后,碱性磷酸酶活性的相对指数最显著。说明成骨细胞对周期性机械拉伸刺激的响应与受载时间相关联,并且受载的成骨细胞经过自身调控后,逐渐适应于新的力学环境,保持一种新的平衡状态。 | 鲜成玉 王远亮 唐丽灵 张兵兵 | 2006 | 北京生物医学工程2006，25，3： | 2 |
| 5 | 新型动态加压交锁髓内针对骨折愈合的影响显示文摘目的探讨新型动态加压交锁髓内针影响骨折愈合的组织学及骨痂内Ⅰ型胶原和骨钙素的表达变化。方法 2周岁青山羊15只,制作双侧股骨干中段横断骨折模型,实验侧以新型动态加压交锁髓内针固定,旨在骨折断端形成持续性动态压应力条件,对照侧以普通髓内针固定。于术后第7、14、30、60、90天在骨折愈合处取材,行组织病理学和RT-PCR等检测。结果组织学检测示实验组骨折间隙内软骨痂基质的钙化、软骨内成骨及编织骨的改建均快于对照组。RT-PCR检测示2组Ⅰ型胶原和骨钙素表达均在骨折术后上升,分别于术后14、30 d时相点达到峰值,实验组明显高于对照组(P<0.05)。结论新型动态加压交锁髓内针能够促进成骨细胞外基质的合成、分泌和软骨内成骨,从而有效加速骨折愈合过程。 | 罗刚 刁鑫伟 李长青 周跃 | 2012 | 第三军医大学学报2012，34，19： | 1 |
| 6 | 载荷作用下成骨细胞的力转导和信号传递机制显示文摘成骨细胞是骨组织中的重要细胞,在骨的形成、发育、改建、修复过程中发挥重要作用。力转导是将力学刺激转化为细胞的生化响应的过程,是引起后续一系列生理反应的基础。信号通路则是一个复杂的网络状信号传递系统,其将信号传入核内引起基因表达的变化。本文将从力转导和信号通路两方面对成骨细胞响应载荷的机制进行综述。 | 宫元伟 闫玉仙 张西正 | 2010 | 中国骨质疏松杂志2010，16，9： | 0 |
| 7 | Integrins mediating bone signal transduction显示文摘Integrin-mediated adhesions play critical roles in diverse cell functions. Integrins offers a platform on which mechanical stimuli, cytoskeletal organization, biochemical signals can concentrate. Mechanical stimuli transmitted by integrins influence the cytoskeleton, in turn, the cytoskeleton influences cell adhesion via integrins, then cell adhesion results in a series of signal transduction cascades. In skeleton, integrins also have a key role for bone resoption by osteoclasts and reformation by osteoblasts. In present review, the proteins involved in integrin signal transduction and integrin signal transduction pathways were discussed, mainly on the basic mechanisms of integrin signaling and the roles of integrins in bone signal transduction, which may give insight into new therapeutic agents to all kinds of skeletal diseases and new strategies for bone tissue engineering. | HEChuanglong WANGYuanliang YANGLihua ZHANGJun | 2004 | Journal of Chongqing University2004，3，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员