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| 1 | RVFL-Based Optical Fiber Intrusion Signal Recognition With Multi-Level Wavelet Decomposition as Feature显示文摘光纤维警告前系统(OFPS ) 逐渐地为监视的管道安全被看作了重要工具之一。侵入信号类型正确地被识别它能减少解决和管道的维护的费用。大多数在 OFPS 的以前的特征抽取方法通常从时间领域的看法被寻找。在一些情况中,然而,在时间领域没有区分的特征。在第一,侵入信号跑展示的纸,挖,并且精选鹤嘴锄被多水平小浪分解在频率领域提取,也就是说,侵入信号被分解成五个乐队。第二,不同频率乐队的平均精力比率被获得,它被看作每种侵入类型的特征。最后,特征样品被送到随机的向量功能连接(RVFL ) 为训练完成信号的分类和鉴定联网。试验性的结果证明算法罐头正确地区分不同侵入信号并且完成更高的识别率。 | Yanping WANG Dianjun GONG Liping PANG Dan YANG | 2018 | Photonic Sensors2018，8，3： | 11 |
| 2 | Application of extension theory in risk zoning of debris flow in Beijing显示文摘The occurrence of debris flow is affected by many factors.Risk zoning of debris flow plays a vital role in the early-warning and prediction of abrupt geological hazards,and exploration of new method is needed in the early-warning and prediction of geological hazards.The extension theory is a new method to solve contradiction matters.Based on extension theory,AHP and GIS,the risk zoning model of debris flow was established in this paper.The result of this research provides a new way in the risk zoning,early-warning and prediction of debris | BAI Liping WANG Yeyao SUN Jiali GONG Bin | 2009 | Global Geology2009，12，1： | 8 |
| 3 | Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference显示文摘Mesenchymal stem cells(MSCs)are multipotent stem cells and capable of differentiating into multiple cell types including osteoblastic,chondrogenic and adipogenic lineages.We previously identified BMP9 as one of the most potent BMPs that induce osteoblastic differentiation of MSCs although exact molecular mechanism through which BMP9 regulates osteogenic differentiation remains to be fully understood.Here,we seek to develop a recombinant adenovirus system to optimally silence mouse BMP9 and then characterize the important role of BMP9 in osteogenic differentiation of MSCs.Using two different siRNA bioinformatic prediction programs,we design five siRNAs targeting mouse BMP9(or simB9),which are expressed under the control of the converging H1 and U6 promoters in recombinant adenovirus vectors.We demonstrate that two of the five siRNAs,simB9-4 and simB9-7,exhibit the highest efficiency on silencing exogenous mouse BMP9 in MSCs.Furthermore,simB9-4 and simB9-7 act synergistically in inhibiting BMP9-induced expression of osteogenic markers,matrix mineralization and ectopic bone formation from MSCs.Thus,our findings demonstrate the important role of BMP9 in osteogenic differentiation of MSCs.The characterized simB9 siRNAs may be used as an important tool to investigate the molecular mechanism behind BMP9 osteogenic signaling.Our results also indicate that recombinant adenovirus-mediated expression of siRNAs is efficient and sustained,and thus may be used as an effective delivery vehicle of siRNA therapeutics. | Shujuan Yan Ruyi Zhang Ke Wu Jing Cui Shifeng Huang Xiaojuan Ji Liping An Chengfu Yuan Cheng Gong Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Bo Liu Rex C.Haydon Michael J.Lee Russell R.Reid Jennifer Moriatis Wolf Qiong Shi Hue H.Luu Tong-Chuan He Yaguang Weng | 2018 | Genes & Diseases2018，5，2： | 8 |
| 4 | Optical forces of focused femtosecond laserpulses on nonlinear optical Rayleigh particles显示文摘The principle of optical trapping is conventionally based on the interaction of optical fields with linear-induced polarizations. However, the optical force originating from the nonlinear polarization becomes significant when nonlinear optical nanoparticles are trapped by femtosecond laser pulses. Herein we develop the time-averaged optical forces on a nonlinear optical nanoparticle using high-repetition-rate femtosecond laser pulses, based on the linear and nonlinear polarization effects. We investigate the dependence of the optical forces on the magnitudes and signs of the refractive nonlinearities. It is found that the self-focusing effect enhances the trapping ability, whereas the self-defocusing effect leads to the splitting of the potential well at the focal plane and destabilizes the optical trap. Our results show good agreement with the reported experimental observations and provide theoretical support for capturing nonlinear optical particles. | LIPING GONG BING GU GUANGHAO RUI YIPING CUI ZHUQING ZHU QIWEN ZHAN | 2018 | Photonics Research2018，6，2： | 8 |
| 5 | miR-486 sustains NF-KB activity by disrupting multiple NF-KB-negative feedback loops显示文摘 | Libing Song Chuyong Lin Hul Gong Chanjuan Wang Liping Liu Jueheng Wu Sha Tao Bo Hu Shi-Yuan Cheng Mengfeng Li Jun Li | 2013 | Cell Research2013，23，2： | 6 |
| 6 | Chemical probing reveals insights into the signaling mechanism of inflammasome activation显示文摘Caspase-1-mediated IL-1 尾生产被二条小径通常控制。像使用费的受体(TLR ) 认出导出病原体的产品并且导致 NF-魏 B 依赖者 pro-IL-1 尾抄写;像点头的受体(NLR ) 装配察觉到细菌的产品 / 危险信号的 caspase-1-activating inflammasome 建筑群。通过一幅指向的化学屏幕,我们识别 bromoxone,一个海洋的自然产品,一特定并且 caspase-1 小径的有势力禁止者。Bromoxone 在包括加 ATP/nigericin, cytosolic DNA, flagellin 和杆菌 anthracis 的 TLR ligands 的多样的煽动性的刺激上是有效的致命的毒素。Bromoxone 高效地也压制激活由细菌的感染的几种类型触发了的 caspase-1。在上游的 Bromoxone 行为或在以一种抄写无关的方式的 inflammasome 的水平。Bromoxone 也由在 TLR-NF-魏 B 小径指向 IKK 在上游的部件禁止 pro-IL-1 尾表示。bromoxone 的唯一的双活动被明确地堵住 Nalp3 inflammasome 激活的已知的 TAK1 禁止者分享。提示了从机械学并且 bromoxone 的药理学性质,我们进一步发现几个已知的 NF-魏 B 禁止者 IKK 在上游的那行为,然而并非下游地指向 IKK 或 IKK 的那些,是不同调停 NLRs 的 caspase-1 激活的有势力 blockers。我们的学习揭开可能的 non-transcriptional 在 NLR (Nalp3 ) 之间的分子的连接调停了 inflammasome 小径和 TLR-NF-魏 B 发信号,并且建议潜在的策略开发新反煽动性的药。 | Yi-Nan Gong Xiaoming Wang Jiayi Wang Zhenxiao Yang Shan Li Jieling Yang Liping Liu Xiaoguang Lei Feng Shao | 2010 | Cell Research2010，20，12： | 5 |
| 7 | Visualization of aging-associated chromatin alterations with an engineered TALE system显示文摘在实时房间的特定的 genomic loci 的可视化在多样的生物过程期间是为在染色质建筑学的动态变化的调查的一个前提,例如细胞的老化。然而,当前的精确 genomic 成像方法被缺乏妨碍荧光灯有高特性和 signal-to-noise 的探针形成对照。我们发现那常规抄写像使活跃之物的受动器(故事) 趋于形成蛋白质总数,从而在成像应用程序损害他们的性能。通过屏蔽,我们发现有故事的那熔化 thioredoxin 阻止了总数形成,开基于故事的 genomic 成像的完整的力量。用熔化 thioredoxin 的故事(TTALE ) ,我们在各种各样的 genomic loci 完成了高质量的成像并且观察联系老化(epi ) 在在人和老鼠早衰模型的 telomeres 和着丝点的 genomic 改变。重要地,我们鉴别 ribosomal DNA 的摩擦为人的老化作为一个分子的标记重复。我们的学习为精确在 vitro 并且在 vivo 监视染色质动力学建立一个简单、柔韧的成像方法。 | Ruotong Ren Liping Deng Yanhong Xue Keiichiro Suzuki Weiqi Zhang Yang Yu Jun Wu Liang Sun Xiaojun Gong Huiqin Luan Fan Yang Zhenyu Ju Xiaoqing Ren Si Wang Hong Tang Lingling Geng Weizhou Zhang Jian Li Jie Qiao Tao Xu Jing Qu Guang-Hui Liu | 2017 | Cell Research2017，27，4： | 4 |
| 8 | Loss of Wip1 aggravates brain injury after ischaemia/reperfusion by overactivating microglia显示文摘Background and purpose The inflammatory response mediated by microglia/macrophages is closely related to cerebral ischaemia/reperfusion injury.Wild-type p53-induced protein phosphatase 1(Wip1),a serine/threonine phosphatase,is expressed in various tissues.A growing number of reports have suggested that Wip1 is a negative regulator of inflammation in peripheral tissue;however,its role in the central nervous system(CNS)remains unclear.This study aimed to clarify whether Wip1 can inhibit CNS inflammation by regulating microglia/macrophage functions after ischaemic injury.Methods A model of middle cerebral artery occlusion and reperfusion was established in mice.CNS inflammation was simulated by lipopolysaccharide treatment of primary microglia.Laser speckle imaging was used to monitor regional cerebral blood flow.Behavioural outcomes were assessed with a TreadScan gait analysis system.TTC staining was used to evaluate the infarct volume,and western blotting and immunofluorescence staining were applied to detect the phenotypical transformation of microglia.ELISA was performed to detect the levels of inflammatory factors.Results Wip1 expression was increased after ischaemia/reperfusion.Wip1-knockout(KO)mice displayed more severe brain injury than wild-type mice,as indicated by aggravated motor dysfunction,greater brain infarct volumes and higher expression of inflammatory cytokines(interleukin-6 and tumour necrosis factor alpha)in the brain.We also found that Wip1 depletion increased microglial/macrophage activation in both in vitro and in vivo models,which all showed activation of microglia/macrophages.Lentivirus-Ppm1d reversed the injury induced by Wip1-KO.Conclusions Our results suggest that Wip1 may inhibit neuroinflammation by inhibiting microglial/macrophage activation after brain ischaemia/reperfusion injury. | Feng Yan Xiang Cheng Ming Zhao Shenghui Gong Ying Han Liping Ding Di Wu Yumin Luo Wei Zuo Lingling Zhu Ming Fan Xunming Ji | 2021 | Stroke & Vascular Neurology2021，6，3： | 4 |
| 9 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 10 | Assessing the ecological vulnerability of protected areas by using Big Earth Data显示文摘The ecological vulnerability of global protected areas(PAs)is linked to the 2030 United Nations Sustainable Development Goals.We developed a novel degree of ecological vulnerability(DEV)index based on Big Earth Data to assess the ecological vulnerability of PAs.This is a transparent,repeatable,large-scale and rapid assessment method.We applied the method to case studies of International Union for Conservation of Nature(IUCN)PAs in the basins of the Aral Sea,the Caspian Sea,the Black Sea and the Lake Baikal.Between 2001 and 2015,the spatial pattern of ecological vulnerability in the study area was relatively stable.The Caspian Sea basin and protected landscape/seascape(category V)showed high ecological vulnerability,while the Black Sea basin and wilderness areas(category Ib)showed low ecological vulnerability.Big Earth Data shows great vitality in PAs ecological vulnerability assessment.Strengthening international cooperation is an important means to break the bottleneck of ecological environment and resource endowment in these important international basins. | Yaomin Zheng Shudong Wang Yue Cao Jinlian Shi Yi Qu Liping Li Tianjie Zhao Zhenguo Niu Rui Yang Peng Gong | 2021 | International Journal of Digital Earth2021，14，11： | 3 |
| 11 | First experimental constraints on WIMP couplings in the effective field theory framework from CDEX显示文摘We present weakly interacting massive particles(WIMPs) search results performed using two approaches of effective field theory from the China Dark Matter Experiment(CDEX), based on the data from both CDEX-1B and CDEX-10 stages. In the nonrelativistic effective field theory approach, both time-integrated and annual modulation analyses were used to set new limits for the coupling of WIMP-nucleon effective operators at 90% confidence level(C.L.) and improve over the current bounds in the low mχregion. In the chiral effective field theory approach, data from CDEX-10 were used to set an upper limit on WIMP-pion coupling at 90% C.L. We for the first time extended the limit to the m_(χ)<6 GeV/c^(2) region. | Yi Wang Zhi Zeng Qian Yue LiTao Yang KeJun Kang YuanJing Li Mehment Agartioglu HaiPeng An JianPing Chang JingHan Chen YunHua Chen JianPing Cheng Cheng Yi Chiang WenHan Dai Zhi Deng ChangHao Fang XinPing Geng Hui Gong QiuJu Guo XuYuan Guo HongJian He Li He ShengMing He JinWei Hu TuChen Huang HanXiong Huang HaiTao Jia LiPing Jia Xi Jiang HauBin Li JianMin Li Jin Li MingXuan Li RenMingJie Li Xia Li YuLan Li Bin Liao FongKay Lin ShinTed Lin ShuKui Liu YanDong Liu Yu Liu YuanYuan Liu ZhongZhi Liu Hao Ma YuCai Mao QiYuan Nie JinHua Ning Hui Pan NingChun Qi Jie Ren XiChao Ruan ChangSong Shang Vivek Sharma Ze She Lakhwinder Singh Monoj Kumar Singh TianXi Sun ChangJian Tang WeiYou Tang Yang Tian GuangFu Wang Li Wang Qing Wang Yu Chen Wang YunXiang Wang Zhen Wang Henry Tsz-King Wong ShiYong Wu YuCheng Wu HaoYang Xing Yin Xu Tao Xue YuLu Yan Nan Yi ChunXu Yu HaiJun Yu JianFeng Yue Ming Zeng BingTao Zhang Lei Zhang FengShou Zhang ZhenYu Zhang KangKang Zhao MingGang Zhao JiFang Zhou ZuYing Zhou JingJun Zhu | 2021 | Science China(Physics,Mechanics & Astronomy)2021，64，8： | 3 |
| 12 | First results on ^(76)Ge neutrinoless double beta decay from CDEX-1 experiment显示文摘We report the first results on ^(76)Ge neutrinoless double beta decay from stage one of the China dark-matter experiment(CDEX).A p-type point-contact high-purity germanium detector with a mass of 994 g has been installed to detect neutrinoless double beta decay events, as well as to directly detect dark matter particles. An exposure of 304 kg d has been analyzed over a wide spectral band from 500 keV to 3 MeV. The average event rate obtained was about 0.012 counts per keV per kg per day over the 2.039 MeV energy range. The half-life of ^(76)Ge neutrinoless double beta decay derived based on this result is T^(0ν)1/2>6.4×10^(22) yr(90% C.L.). An upper limit on the effective Majorana-neutrino mass of 5.0 eV has been achieved. | Li Wang Qian Yue KeJun Kang JianPing Cheng YuanJing Li TszKing Henry Wong ShinTed Lin JianPing Chang JingHan Chen QingHao Chen YunHua Chen Zhi Deng Qiang Du Hui Gong Li He QingJu He JinWei Hu HanXiong Huang TengRui Huang LiPing Jia Hao Jiang HauBin Li Hong Li JianMin Li Jin Li Jun Li Xia Li XueQian Li YuLan Li FongKay Lin ShuKui Liu Hao Ma JingLu Ma XingYu Pan Jie Ren XiChao Ruan ManBin Shen Vivek Sharma Lakhwinder Singh Manoj Kumar Singh Manoj Kumar Singh Arun Kumar Soma ChangJian Tang WeiYou Tang ChaoHsiung Tseng JiMin Wang Qing Wang ShiYong Wu YuCheng Wu HaoYang Xing Yin Xu Tao Xue LiTao Yang SongWei Yang Nan Yi ChunXu Yu HaiJun Yu WeiHe Zeng XiongHui Zeng Zhi Zeng Lan Zhang YunHua Zhang MingGang Zhao Wei Zhao JiFang Zhou ZuYing Zhou JingJun Zhu WeiBin Zhu ZhongHua Zhu | 2017 | Science China(Physics,Mechanics & Astronomy)2017，60，7： | 3 |
| 13 | Steady State Rheological Characteristic of Semisolid Magnesium Alloy显示文摘在为半固体 AZ91D 的不同温度,紧张率和成立时间的等温的压缩实验, Zr 与更高的固体修改了 AZ91D 和 MB15 合金体积部分被使用 Gleeble-1500D 模拟器和真压力紧张曲线执行是表观粘度对温度的 givendirectly.The 关系,砍率和三种半固体镁合金的成立时间,以及等温的稳定的州的 rheologicalcharacteristic 和机械行为被学习。结果证明三镁合金有半固体 MB15 的逻辑特征是的 shear-thinning.The 变阻器的特征与半固体 MB15 更高有的半固体 AZ91D.The 的不同表观粘度和变丑抵抗。 | Fengyun YAN Liping SUN Yi GONG Yuan HAO | 2007 | Journal of Materials Science & Technology2007，23，5： | 3 |
| 14 | Fluorouracil-based preoperative chemoradiotherapy with or without oxaliplatin for stage Ⅱ/Ⅲ rectal cancer:a 3-year follow-up study显示文摘Background:Fluorouracil-based preoperative chemoradiotherapy has become the standard treatment for stage II/III rectal cancer.In order to improve the overall survival(OS) and disease-free survival(DFS),we added oxaliplatin to the standard treatment,and compared the effectiveness of these two treatment patterns.Methods:A total of 206 patients enrolled in the prospective study had histologically confirmed rectal cancer of clinical stage II/III during July 2007 to July 2010.They were randomized into the experimental group received oxaliplatin and capecitabine in combination with radiotherapy,and the control group received capecitabine in combination with radiotherapy.All patients received surgery in 6-10 weeks after chemoradiotherapy and adjuvant chemotherapy with m FOLFOX6.The primary endpoints were DFS and OS,and the secondary endpoints included toxicity,compliance,and histopathological response.Results:The 3-year OS in the experimental group and the control group was 90.29% vs.86.41%(P>0.05),and the 3-year DFS was 80.58% vs.69.90%(P>0.05).The pathological complete remission(p CR) rates were 23.30% and 19.42%,respectively(P=0.497).The 3-year local recurrence rates were 4.85% vs.5.83%(P=0.694),and the 3-year distant metastasis rates were 16.50% and 28.16%,respectively(P=0.045).There were no significant differences in most grade 3-4 toxicities between two groups,however,grade 3-4 diarrhea occurred in 16.50%(17/103) of the experimental group,compared with 6.80%(7/103) of the control group(P=0.030).Also,the total grade 3-4 acute toxicity showed a significant difference(10.68% vs.21.36%,P=0.037).Conclusions:The experimental treatment did not lead significantly improved OS and DFS,and thus longer follow-up is warranted for our patient cohort.Adding oxaliplatin to capecitabine-based preoperative chemoradiotherapy can significantly reduce metastasis,but has only minimal impact on local recurrence.Although grade 3-4 toxicity rate increased(primarily gastrointestinal toxicity),patients can stand to be followed up with allopathic treatment. | Dexin Jiao Rui Zhang Zhiqiang Gong Fang Liu Yue Chen Qinrui Yu Liping Sun Hongyan Duan Shendong Zhu Fei Liu Jian Wang Jianhui Jia | 2015 | Chinese Journal of Cancer Research2015，27，6： | 2 |
| 15 | SARS-CoV-2 Omicron RBD shows weaker binding affinity than the currently dominant Delta variant to human ACE2显示文摘Dear Editor,SARS-coronavirus-2(SARS-CoV-2)Omicron variant(B.1.1.529)is of great concern to the world due to its multiple mutations that may have an impact on transmissibility and immune evasion.1 Compared to the wild type(WT),Omicron carries as many as 30 single point mutations,3 deletion mutation and one insertion mutation on its spike protein.Strikingly,there are 15 mutations observed in the Omicron receptor-binding domain(RBD),10 of which are in the receptor-binding motif(RBM)that human angiotensin-converting enzyme 2(ACE2)and most monoclonal antibodies(mAbs)interact directly with.As a comparison,the currently dominant variant Delta(B.1.617.2)has only 2 mutations(L452R and T478K)in its RBM and additional K417N and E484K mutations sometimes.Therefore,Omicron variant may significantly impact the binding affinity to ACE2 and effectiveness of currently available mAbs.Consequently,Omicron mutant has aroused wide concern,many countries have taken measures on entry restrictions to prevent its rapid spread.However,the transmissibility and immune evasion risk of Omicron have not been properly evaluated. | Leyun Wu Liping Zhou Mengxia Mo Tingting Liu Chengkun Wu Chunye Gong Kai Lu Likun Gong Weiliang Zhu Zhijian Xu | 2022 | Signal Transduction and Targeted Therapy2022，7，2： | 2 |
| 16 | CUL4B negatively regulates Toll-like receptor-triggered proinflammatory responses by repressing Pten transcription显示文摘Toll-like receptors (TLRs) play critical roles in innate immunity and inflammation. The molecular mechanisms by which TLR signaling is fine-tuned remain to be completely elucidated. Cullin 4B (CUL4B), which assembles the CUL4B-RING E3 ligase complex (CRL4B), has been shown to regulate diverse developmental and physiological processes by catalyzing monoubiquitination for histone modification or polyubiquitination for proteasomal degradation. Here, we identified the role of CUL4B as an intrinsic negative regulator of the TLR-triggered inflammatory response. Deletion of CUL4B in macrophages increased the production of proinflammatory cytokines and decreased anti-inflammatory cytokine IL-10 production in response to pathogens that activate TLR3, TLR4, or TLR2. Myeloid cell-specific Cul4b knockout mice were more susceptible to septic shock when challenged with lipopolysaccharide, polyinosinic-polycytidylic acid or Salmonella typhimurium infection. We further demonstrated that enhanced TLR-induced inflammatory responses in the absence of CUL4B were mediated by increased GSK3β activity. Suppression of GSK3β activity efficiently blocked the TLR-triggered increase in proinflammatory cytokine production and attenuated TLR-triggered death in Cul4b mutant mice. Mechanistically, CUL4B was found to negatively regulate TLR-triggered signaling by epigenetically repressing the transcription of Pten, thus maintaining the anti-inflammatory PI3K-AKT-GSK3β pathway. The upregulation of PTEN caused by CUL4B deletion led to uncontrolled GSK3β activity and excessive inflammatory immune responses. Thus, our findings indicate that CUL4B functions to restrict TLR-triggered inflammatory responses through regulating the AKT-GSK3β pathway. | Yu Song Peishan Li Liping Qin Zhiliang Xu Baichun Jiang Chunhong Ma Changshun Shao Yaoqin Gong | 2021 | Cellular & Molecular Immunology2021，18，2： | 2 |
| 17 | An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip显示文摘CRISPR-Cas12a system has been shown promising for nucleic acid diagnostics due to its rapid,portable and accurate features.However,cleavage of the amplicons and primers by the cis-and trans-activity of Cas12a hinders the attempts to integrate the amplification and detection into a single reaction.Through phosphorothioate modification of primers,we realized onepot detection with high sensitivity using plasmids of SARS-CoV-2,HPV16 and HPV18.We also identified the activated Cas12a has a much higher affinity to C nucleotide-rich reporter than others.By applying such reporters,the reaction time required for a lateral-flow readout was significantly reduced.Furthermore,to improve the specificity of the strip-based assay,we created a novel reporter and,when combined with a customized gold-nanopaticle strip,the readout was greatly enhanced owing to the elimination of the nonspecific signal.This established system,termed Targeting DNA by Cas12a-based Eye Sight Testing in an Onepot Reaction(TESTOR),was validated using clinical cervical scrape samples for human papillomaviruses(HPVs)detection.Our system represents a general approach to integrating the nucleic acid amplification and detection into a single reaction in CRISPR-Cas systems,highlighting its potential as a rapid,portable and accurate detection platform of nucleic acids. | Jiaojiao Gong Lijuan Kan Xiuming Zhang Ying He Jiaqiang Pan Liping Zhao Qianyun Li Menghao Liu Jie Tian Sili Lin Zhouyu Lu Liang Xue Chaojun Wang Guanghui Tang | 2021 | Bioactive Materials2021，6，12： | 1 |
| 18 | Distinct neuron populations for simple and compound calls in the primary auditory cortex of awake marmosets显示文摘Marmosets are highly social non-human primates that live in families.They exhibit rich vocalization,but the neural basis underlying this complex vocal communication is largely unknown.Here we report the existence of specific neuron populations in marmoset A1 that respond selectively to distinct simple or compound calls made by conspecific marmosets.These neurons were spatially dispersed within A1 but distinct from those responsive to pure tones.Call-selective responses were markedly diminished when individual domains of the call were deleted or the domain sequence was altered,indicating the importance of the global rather than local spectral-temporal properties of the sound.Compound call-selective responses also disappeared when the sequence of the two simple-call components was reversed or their interval was extended beyond 1 s.Light anesthesia largely abolished call-selective responses.Our findings demonstrate extensive inhibitory and facilitatory interactions among call-evoked responses,and provide the basis for further study of circuit mechanisms underlying vocal communication in awake non-human primates. | Huan-huan Zeng Jun-feng Huang Jun-ru Li Zhiming Shen Neng Gong Yun-qing Wen Liping Wang Mu-ming Poo | 2021 | National Science Review2021，8，11： | 1 |
| 19 | Bagging null space locality preserving disctiminant classifiers for face recognition显示文摘 | Yang Liping Gong Weiguo Gu Xiaohna | 2009 | Pattern Recognition2009，42，9： | 1 |
| 20 | Anti-inflammatory Effects of Rebamipide According to Helicobacter pylori Status in Patients with Chronic Erosive Gastritis: A Randomized Sucralfate-Controlled Multicenter Trial in China—STARS Study显示文摘 | Yiqi Du Zhaoshen Li Xianbao Zhan Jie Chen Jun Gao Yanfang Gong Jianlin Ren Liping He Zhijian Zhang Xiaozhong Guo Jianshen Wu Zibin Tian Ruihua Shi Bo Jiang Dianchun Fang Youming Li | 2008 | Digestive Diseases and Sciences2008，，11： | 1 |
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