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您的检索式：作者名="Yaguang Weng"
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| 1 | Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference显示文摘Mesenchymal stem cells(MSCs)are multipotent stem cells and capable of differentiating into multiple cell types including osteoblastic,chondrogenic and adipogenic lineages.We previously identified BMP9 as one of the most potent BMPs that induce osteoblastic differentiation of MSCs although exact molecular mechanism through which BMP9 regulates osteogenic differentiation remains to be fully understood.Here,we seek to develop a recombinant adenovirus system to optimally silence mouse BMP9 and then characterize the important role of BMP9 in osteogenic differentiation of MSCs.Using two different siRNA bioinformatic prediction programs,we design five siRNAs targeting mouse BMP9(or simB9),which are expressed under the control of the converging H1 and U6 promoters in recombinant adenovirus vectors.We demonstrate that two of the five siRNAs,simB9-4 and simB9-7,exhibit the highest efficiency on silencing exogenous mouse BMP9 in MSCs.Furthermore,simB9-4 and simB9-7 act synergistically in inhibiting BMP9-induced expression of osteogenic markers,matrix mineralization and ectopic bone formation from MSCs.Thus,our findings demonstrate the important role of BMP9 in osteogenic differentiation of MSCs.The characterized simB9 siRNAs may be used as an important tool to investigate the molecular mechanism behind BMP9 osteogenic signaling.Our results also indicate that recombinant adenovirus-mediated expression of siRNAs is efficient and sustained,and thus may be used as an effective delivery vehicle of siRNA therapeutics. | Shujuan Yan Ruyi Zhang Ke Wu Jing Cui Shifeng Huang Xiaojuan Ji Liping An Chengfu Yuan Cheng Gong Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Bo Liu Rex C.Haydon Michael J.Lee Russell R.Reid Jennifer Moriatis Wolf Qiong Shi Hue H.Luu Tong-Chuan He Yaguang Weng | 2018 | Genes & Diseases2018，5，2： | 8 |
| 2 | BMP9 inhibits the bone metastasis of breast cancer cells by downregulating CCN2 (connective tissue growth factor, CTGF) expression显示文摘 | Wei Ren Xiaoxiao Sun Ke Wang Honglei Feng Yuehong Liu Chang Fei Shaoheng Wan Wei Wang Jinyong Luo Qiong Shi Min Tang Guowei Zuo Yaguang Weng Tongchuan He Yan Zhang | 2014 | Molecular Biology Reports2014，，3： | 1 |
| 3 | Upregulation of miR-195 increases the sensitivity of breast cancer cellsto Adriamycin treatment through inhibition of Raf-1显示文摘 | Ge Yang Daoquan Wu Jing Zhu Ou Jiang Qiong Shi Jie Tian Yaguang Weng | 2013 | Oncology Reports2013，，2： | 1 |
| 4 | Corrigendum to “Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference” [Genes & Diseases 5(2018):172–184]显示文摘The authors regret having several image assembly errors.Specifically,in Figure 3A panel b,the image for 'AdsimB9-4 only'group was erroneously duplicated with an overlapping image from the'AdRFp'group;and the image for'AdsimB9-1+BMP9'groupwas erroneouslyduplicatedwithan overlapping image from'AdsimB9-8+BMP9'group.In Figure 4Apanel a,the images for'BMP9'group and 'BMP9+simB9-4'group were erroneously duplicated with an overlapping image from'simB9-4'group.In Figure 5A,the image for'BMP9+simB9-4/Day3'group was erroneously duplicated with an overlapping image from'BMP9+simB9-7/Day3'group;and the image for'BMP9+simB9-4/Day5'group was erroneously duplicated with an overlapping image from an unrelated experiment.In Figure 6B,the image for'BMP9+simB9-7/Day 11'group was erroneously duplicated with an overlapping image from the'BMP9+simB9-4/Day 11'group. | Shujuan Yan Ruyi Zhang Ke Wu Jing Cui Shifeng Huang Xiaojuan Ji Liping An Chengfu Yuan Cheng Gong Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Bo Liu Rex C. Haydon Michael J. Lee Russell R. Reid Jennifer Moriatis Wolf Qiong Shi Hue H. Luu Tong-Chuan He Yaguang Weng | 2023 | Genes & Diseases2023，10，2： | 0 |
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