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| 1 | Bioengineering bacterial encapsulin nanocompartments as targeted drug delivery system显示文摘The development of Drug Delivery Systems(DDS)has led to increasingly efficient therapies for the treatment and detection of various diseases.DDS use a range of nanoscale delivery platforms produced from polymeric of inorganic materials,such as micelles,and metal and polymeric nanoparticles,but their variant chemical composition make alterations to their size,shape,or structures inherently complex.Genetically encoded protein nanocages are highly promising DDS candidates because of their modular composition,ease of recombinant production in a range of hosts,control over assembly and loading of cargo molecules and biodegradability.One example of naturally occurring nanocompartments are encapsulins,recently discovered bacterial organelles that have been shown to be reprogrammable as nanobioreactors and vaccine candidates.Here we report the design and application of a targeted DDS platform based on the Thermotoga maritima encapsulin reprogrammed to display an antibody mimic protein called Designed Ankyrin repeat protein(DARPin)on the outer surface and to encapsulate a cytotoxic payload.The DARPin9.29 chosen in this study specifically binds to human epidermal growth factor receptor 2(HER2)on breast cancer cells,as demonstrated in an in vitro cell culture model.The encapsulin-based DDS is assembled in one step in vivo by co-expressing the encapsulin-DARPin9.29 fusion protein with an engineered flavin-binding protein mini-singlet oxygen generator(MiniSOG),from a single plasmid in Escherichia coli.Purified encapsulin-DARPin_miniSOG nanocompartments bind specifically to HER2 positive breast cancer cells and trigger apoptosis,indicating that the system is functional and specific.The DDS is modular and has the potential to form the basis of a multi-receptor targeted system by utilising the DARPin screening libraries,allowing use of new DARPins of known specificities,and through the proven flexibility of the encapsulin cargo loading mechanism,allowing selection of cargo proteins of choice. | Alexander Van de Steen Rana Khalife Noelle Colant Hasan Mustafa Khan Matas Deveikis Saverio Charalambous Clare M.Robinson Rupali Dabas Sofia Esteban Serna Diana A.Catana Konstantin Pildish Vladimir Kalinovskiy Kenth Gustafsson Stefanie Frank | 2021 | Synthetic and Systems Biotechnology2021，6，3： | 2 |
| 2 | Determining the Value of Information for Different Partners in the Supply Chain显示文摘 | Kenth Lumsden Vahid Mirzabeiki | 2008 | Inter- national Journal of Physical Distribution & Logistics Manage- ment2008，38，9： | 1 |
| 3 | Adaption to climatic impacts on the supply and demand for water显示文摘 | Kenth D F | 1997 | Climatic Change1997，37，： | 1 |
| 4 | Performance issues of Smart Transportation Management systems显示文摘 | Gunnar Stefansson Kenth Lumsden | 2008 | International Journal of Productivity and Performance Management2008，，1： | 1 |
| 5 | Multi-year remote-sensing measurements of gasoline light-duty vehicle emissions on a freeway ramp显示文摘 | Ake Sjodin Kenth Andreasson | 2000 | Atmospheric Environment2000，34，： | 1 |
| 6 | Soot processes in compression ignition engines 显示文摘 | Dale R T Kenth I S | 2007 | Progress in Energy and Combustion Science2007，33，3： | 1 |
| 7 | Testosterone inhibits and induces apoptosis in rat seminiferous tubules in a stage-specific manner:in situ quantification in squash preparations after administrtion of Ethane Dimethane sulfonate显示文摘 | Kenth H Harri H Martti P | 1995 | Endocrinology1995，136，8： | 1 |
| 8 | Improving the efficiency of the hub and spoke system for the SKF Eu- ropean distribution network 显示文摘 | Kenth Lumsden Fabfizio Dallari Remigio Ruggeri | 1999 | International Journal of Physical Distribution & Logistics Management1999，29，1： | 1 |
| 9 | Limited polymorphism in the first domain of the rat MHC class II RT1-D molecule显示文摘 | M. Vestberg Ulrica Brunsberg Kristin Bergsteinsdottir Mikael Karlsson Kenth Gustafsson Dirk Wedekind Hans Hedrich Rikard Holmdahl | 1998 | Immunogenetics1998，，5： | 1 |
| 10 | γδ T cells for cancer immunotherapy显示文摘 | Jonathan PH Fisher Jennifer Heuijerjans Mengyong Yan Kenth Gustafsson John Anderson | 2014 | OncoImmunology2014，，： | 1 |
| 11 | Concerted evolution in a segment of the first domain exon of polymorphic MHC class II β loci显示文摘 | Leif Andersson Kenth Gustafsson Ann-Kristin Jonsson Lars Rask | 1991 | Immunogenetics1991，，4： | 1 |
| 12 | Soot Processes in Compression Ig- nition Engines 显示文摘 | Dale R T Kenth I S | 2007 | Progress in Energy and Combustion Science2007，33，27： | 1 |
| 13 | Metal emissions from road traffic and the influence of resuspension—results from two tunnel studies显示文摘 | John Sternbeck ?ke Sj?din Kenth Andréasson | 2002 | Atmospheric Environment2002，，30： | 1 |
| 14 | Results from the International Conference of Experts on Intra-abdominal Hypertension and Abdominal Compartment Syndrome. I. Definitions显示文摘 | Manu L. N. G. Malbrain Michael L. Cheatham Andrew Kirkpatrick Michael Sugrue Michael Parr Jan Waele Zsolt Balogh Ari Lepp?niemi Claudia Olvera Rao Ivatury Scott D’Amours Julia Wendon Ken Hillman Kenth Johansson Karel Kolkman Alexander Wilmer | 2006 | Intensive Care Medicine2006，，11： | 1 |
| 15 | Crystal structure of the measles virus phosphoprotein domain prsponsible for the induced folding of the C-terminal domain of the nucleoprotein 显示文摘 | Kenth Johansson Jean Marie Bourhis Valerie Campanacci etal | 2003 | The journal of biological chemistry2003，278，44： | 1 |
| 16 | Apoptosis in Human Acute Myocardial Infarction显示文摘 | Antti Saraste Kari Pulkki Markku Kallajoki Kenth Henriksen Martti Parvinen Liisa-Maria Voipio-Pulkki | 1997 | Circulation1997，，2： | 1 |
| 17 | Soot processes in compression ignition engines显示文摘 | Dale R. Tree Kenth I. Svensson | 2006 | Progress in Energy and Combustion Science2006，，3： | 1 |
| 18 | Soot processes in compression ignition engines显示文摘 | DALE R T KENTH I S | 2007 | Progress in Energy and Combustion Science2007，33，： | 1 |
| 19 | The Dry Port Concept: Connecting Container Seaports with the Hinterland 显示文摘 | Violeta Roso Johan Woxenius Kenth Lumsden | 2009 | Journal of Transport Geography2009，，17： | 1 |
| 20 | Sudden bereavement in acute care settings显示文摘 | Mcdowell J | 2004 | Nuts Stand2004，19，6： | 1 |
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