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| 1 | Developing a bacteriophage cocktail for biocontrol of potato bacterial wilt显示文摘Bacterial wilt is a devastating disease of potato and can cause an 80% production loss. To control wilt using bacteriophage therapy, we isolated and characterized twelve lytic bacteriophages from different water sources in Kenya and China. Based on the lytic curves of the phages with the pathogen Ralstonia solanacearum, one optimal bacteriophage cocktail, P1, containing six phage isolations was formulated and used for studying wilt prevention and treatment efficiency in potato plants growing in pots. The preliminary tests showed that the phage cocktail was very effective in preventing potato bacterial wilt by injection of the phages into the plants or decontamination of sterilized soil spiked with R. solanacearum. Eighty percent of potato plants could be protected from the bacterial wilt(caused by R. solanacearum reference strain GIM1.74 and field isolates), and the P1 cocktail could kill 98% of live bacteria spiked in the sterilized soil at one week after spraying.However, the treatment efficiencies of P1 depended on the timing of application of the phages, the susceptibility of the plants to the bacterial wilt, as well as the virulence of the bacteria infected,suggesting that it is important to apply the phage therapy as soon as possible once there are early signs of the bacterial wilt. These results provide the basis for the development of bacteriophagebased biocontrol of potato bacterial wilt as an alternative to the use of antibiotics. | Cuihua Wei Junli Liu Alice Nyarnbura Maina Francis B Mwaura Junping Yu Chenghui Yan Ruofang Zhang Hongping Wei | 2017 | Virologica Sinica2017，32，6： | 8 |
| 2 | The treatment of black-odorous water using tower bipolar electro-flocculation including the removal of phosphorus,turbidity,sulfion,and oxygen enrichment显示文摘The large amount of municipal wastewater discharged into urban rivers sometimes exceeds the rivers’self-purification capacity leading to black-odorous polluted water.Electro-flocculation has emerged as a powerful remediation technology.Electro-flocculation in a bubble column tower with a bipolar electrode(BPE)was tested in an attempt to overcome the high resistance and weak gas-floatation observed with a monopolar electrode(MPE)in treating such water.The BPE reactor tested had a Ti/Ta2O_(5)-IrO_(2)anode and a graphite cathode with an iron or aluminum bipolar electrode suspended between them.It was tested for its ability to reduce turbidity,phosphate and sulphion and to increase the concentration of dissolved oxygen.The inclusion of the bipolar electrode was found to distinctly improved the system’s conductivity.The system’s electro-flocculation and electrical floatation removed turbidity,phosphate and sulphion completely,and the dissolved oxygen level improved from 0.29 to 6.28 mg/L.An aluminum bipolar electrode performed better than an iron one.Changes in the structure of the microbial community confirmed a significant improvement in water quality. | Huan He Qinjin Yu Chaochao Lai Chen Zhang Muhan Liu Bin Huang Hongping Pu Xuejun Pan | 2021 | Frontiers of Environmental Science & Engineering2021，15，2： | 5 |
| 3 | Bacteriophages Isolated in China for the Control of Pectobacterium carotovorum Causing Potato Soft Rot in Kenya显示文摘Soft rot is an economically significant disease in potato and one of the major threats to sustainable potato production.This study aimed at isolating lytic bacteriophages and evaluating methods for and the efficacy of applying phages to control potato soft rot caused by Pectobacterium carotovorum.Eleven bacteriophages isolated from soil and water samples collected in Wuhan,China,were used to infect P.carotovorum host strains isolated from potato tubers showing soft rot symptoms in Nakuru county,Kenya.The efficacy of the phages in controlling soft rot disease was evaluated by applying individual phage strains or a phage cocktail on potato slices and tubers at different time points before or after inoculation with a P.carotovorum strain.The phages could lyse 20 strains of P.carotovorum,but not Pseudomonas fluorescens control strains.Among the 11 phages,Pectobacterium phage Wc5r,interestingly showed cross-activity against Pectobacterium atrosepticum and two phage-resistant P.carotovorum strains.Potato slice assays showed that the phage concentration and timing of application are crucial factors for effective soft rot control.Phage cocktail applied at a concentration of 1×10^9 plaque-forming units per milliliter before or within an hour after bacterial inoculation on potato slices,resulted in>90%reduction of soft rot symptoms.This study provides a basis for the development and application of phages to reduce the impact of potato soft rot disease. | Peter Muturi Junping Yu Alice Nyambura Maina Samuel Kariuki Frantis B.Mwaura Hongping Wei | 2019 | Virologica Sinica2019，34，3： | 4 |
| 4 | Amorphous TiO_2-supported Keggin-type ionic liquid catalyst catalytic oxidation of dibenzothiophene in diesel显示文摘Supported ionic liquid(IL) catalysts [C_nmim]_3PMo_(12)O_(40)/Am TiO_2(amorphous TiO_2) were synthesized through a one-step method for extraction coupled catalytic oxidative desulfurization(ECODS) system. Characterizations such as FTIR, DRS,wide-angle XRD, N_2 adsorption–desorption and XPS were applied to analyze the morphology and Keggin structure of the catalysts. In ECODS with hydrogen peroxide as the oxidant, it was found that ILs with longer alkyl chains in the cationic moiety had a better effect on the removal of dibenzothiophene. The desulfurization could reach 100% under optimal conditions, and GC–MS analysis was employed to detect the oxidized product after the reaction. Factors affecting the desulfurization efficiencies were discussed, and a possible mechanism was proposed. In addition, cyclic experiments were also conducted to investigate the recyclability of the supported catalyst. The catalytic activity of [C_(16)mim]_3 PMo_(12)O_(40)/Am TiO_2 only dropped from 100% to 92.9% after ten cycles, demonstrating the good recycling performance of the catalyst and its potential industrial application. | Zhendong Yu Donghui Wang Suhang Xun Minqiang He Ruliang Ma Wei Jiang Hongping Li Wenshuai Zhu Huaming Li | 2018 | Petroleum Science2018，15，4： | 3 |
| 5 | Systematic Characterization of Long Non-Coding RNAs and Their Responses to Drought Stress in Dongxiang Wild Rice显示文摘Long non-coding RNAs(lncRNAs) play important roles in response to various biotic and abiotic stresses. So far, systematic identification and characterization of lncRNAs have been reported in a few model plant species and major crops, but their roles in abiotic stress response have not yet been reported in common wild rice(Oryza rufipogon). Dongxiang wild rice(DXWR) possesses a high degree of drought resistance and has been well recognized as a precious genetic resource for drought resistant rice breeding. We presented the reference catalog of 1 655 novel lncRNA transcripts in DXWR using strand-specific RNA sequencing and bioinformatics approaches. Meanwhile, a total of 1092 lncRNAs were determined as differentially expressed lncRNAs under drought stress. Quantitative real-time PCR results exhibited a high concordance with RNA sequencing data, which confirmed that the expression patterns of lncRNAs based on RNA sequencing were highly reliable. Furthermore, 8 711 transcripts were predicted as target genes of the differentially expressed lncRNAs. Functional annotation analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases showed that the target genes were significantly enriched in cellular and metabolic processes, cell part, binding and plant hormone signal transduction, as well as many other terms related to abiotic stress resistance. These results expanded our understanding of lncRNA biology and provided candidate regulators for genetic improvement of drought resistance in rice cultivars. | QI Weidong CHEN Hongping YANG Zuozhen HU Biaolin LUO Xiangdong AI Bing LUO Yuan HUANG Yu XIE Jiankun ZHANG Fantao | 2020 | Rice science2020，27，1： | 3 |
| 6 | Enriched Opportunistic Pathogens Revealed by Metagenomic Sequencing Hint Potential Linkages between Pharyngeal Microbiota and COVID-19显示文摘As a respiratory tract virus,SARS-CoV-2 infected people through contacting with the upper respiratory tract first.Previous studies indicated that microbiota could modulate immune response against pathogen infection.In the present study,we performed metagenomic sequencing of pharyngeal swabs from eleven patients with COVID-19 and eleven Non-COVID-19 patients who had similar symptoms such as fever and cough.Through metagenomic analysis of the above two groups and a healthy group from the public data,there are 6502 species identified in the samples.Specifically,the Pielou index indicated a lower evenness of the microbiota in the COVID-19 group than that in the Non-COVID-19 group.Combined with the linear discriminant analysis(LDA)and the generalized linear model,eighty-one bacterial species were found with increased abundance in the COVID-19 group,where 51 species were enriched more than 8 folds.The top three enriched genera were Streptococcus,Prevotella and Campylobacter containing some opportunistic pathogens.More interestingly,through experiments,we found that two Streptococcus strains,S.suis and S.agalactiae,could stimulate the expression of ACE2 of Vero cells in vitro,which may promote SARS-CoV-2 infection.Therefore,these enriched pathogens in the pharynxes of COVID-19 patients may involve in the virus-host interactions to affect SARS-CoV-2 infection and cause potential secondary bacterial infections through changing the expression of the viral receptor ACE2 and/or modulate the host’s immune system. | Dongyan Xiong Caroline Muema Xiaoxu Zhang Xinming Pan Jin Xiong Hang Yang Junping Yu Hongping Wei | 2021 | Virologica Sinica2021，36，5： | 3 |
| 7 | Performance study on T-stub connected semi-rigid joint between rectangular tubular columns and H-shaped steel beams显示文摘This paper investigates the performance of T-stub connected semi-rigid joint of rectangular tubular columns and H-shaped steel beams.The finite element analysis software ABAQUS is used to analyze the nonlinear performance of the joint under monotonic loading.Meanwhile,the dimensions of T-stub,column and beam are considered as analytic parameters to discuss the performance of the joint.The analysis shows that the thickness and the length of T-stub webs,the height of beam section,bolt diameter,shear connector and the preloaded force affect the performance of the joint largely,and the thickness of the steel tube,the thickness and length of T-stub flange,bolt spacing have relatively little influences on the performance of the joint.The research results indicate that this joint is semi-rigid joint. | Guochang LI Hongping YU Chen FANG | 2013 | Frontiers of Structural and Civil Engineering2013，7，3： | 2 |
| 8 | Piezo1 regulates migration and invasion of breast cancer cells via modulating cell mechanobiological properties显示文摘Cell migration and invasion are two essential processes during cancer metastasis. Increasing evidence has shown that the Piezo1 channel is involved in mediating cell migration and invasion in some types of cancers. However, the role of Piezo1 in the breast cancer and its underlying mechanisms have not been clarified yet. Here, we show that Piezo1 is high-expressed in breast cancer cell (BCC) lines, despite its complex expression in clinical patient database. Piezo1 knockdown (Piezo1-KD) promotes unconfined BCC migration, but impedes confined cell migration. Piezo1 may mediate BCC migration through the balances of cell adhesion, cell stiffness, and contractility. Furthermore, Piezo1-KD inhibits BCC invasion by impairing the invadopodium formation and suppressing the expression of metalloproteinases (MMPs) as well. However, the proliferation and cell cycle of BCCs are not significantly affected by Piezo1. Our study highlights a crucial role of Piezo1 in regulating migration and invasion of BCCs, indicating Piezo1 channel might be a new prognostic and therapeutic target in BCCs. | Yang Yu Xiao’an Wu Sisi Liu Hongping Zhao Bo Li Hucheng Zhao Xiqiao Feng | 2021 | Acta Biochimica et Biophysica Sinica2021，53，1： | 2 |
| 9 | Synergistic Action of Clonorchiasis,HBV Infection and Alcohol Consumption on Primary Hepatocellular Carcinoma显示文摘OBJECTIVE It has been recognized that HBV infection andalcohol consumption are two important risk factors for primaryhepatocellular carcinoma (HCC). Recently, the role of clonorchiasisas a risk factor for HCC is controversial. We aimed to investigatewhether these factors increase the risk of HCC in Guangxi, China.METHODS A hospital-based, case-control study of HCCwas conducted from July 2005 to July 2007. We enrolled 500consecutive patients with HCC as an experimental group and 500patients without tumor in liver as a control group. The risk factorsthat the patients were exposed to were assessed.RESULTS Comparing the risks of developing the HCC, wefound out the following results. The risk of developing HCC forthe patients with clonorchiasis was 5 folds of that for the patientswithout clonorchiasis (OR = 5.0; 95% CI: 3.1-8.1), and the risk forthe patients with alcohol consumption was 3 folds of that for thepatients without drinking alcohol (OR = 3.4; 95% CI: 2.3-4.9), andsimilarly, the risk for the patients with HBV infection was 21 timesof that for the patients without HBV infection (OR = 20.6; 95% CI:14.3-29.7). According to crossover analysis, there was significantinteraction among clonorchiasis, HBV infection and alcoholconsumption, with synergistic indices greater than 1. The etiologicfractions attributed to these interactions [EF (A x B)] are 0.7465,0.5789 and 0.5506, respectively.CONCLUSION Clonorchiasis, HBV infection and heavy alcoholconsumption are independent risk factors for developing HCC inour population in Guangxi, and as they can interact synergistically,the risk of developing HCC is increased. Data from this study mayindicate new prevention strategies of developing HCC in high-riskindividuals. | Shengkui Tan Xiaoqiang Qiu Hongping Yu Xiaoyun Zeng Zengming Xiao Lequn Li Qiuan Zhong | 2009 | Chinese Journal of Clinical Oncology2009，6，2： | 2 |
| 10 | BaYOBO_(3):A deep-ultraviolet rare-earth oxy-borate with a large second harmonic generation response显示文摘Deep-ultraviolet(UV)nonlinear optical(NLO)crystals with excellent performances are of great importance in laser science and technology.However,to design and synthesize an ideal deep-UV NLO material with a balance between second harmonic generation(SHG)response and UV cut-off edge is still a huge challenge.Herein,a new oxy-borate BaYOBO_(3)(BaYBO)was designed and synthesized by substituting Be^(2+)with Y^(3+)from Sr_(2)Be_(2)B_(2(O_(7).The BaYBO features a stable three-dimensional framework and satisfies the property balance between a large SHG response(~2.6×KH_(2)PO_(4)KDP))and a short UV cut-off edge(<190 nm)as a promising deep-UV NLO candidate.The study of structure-property relationship indicates that the large SHG response of BaYBO is mainly attributed to the coplanar arrangement and large number density of BO_(3 )triangles.These results demonstrate that substituting Be^(2+)with Y^(3+)cations is also a feasible strategy for developing new deep-UV NLO crystals. | Mengen Gao Hongping Wu Hongwei Yu Zhanggui Hu Jiyang Wang Yicheng Wu | 2021 | Science China Chemistry2021，64，7： | 1 |
| 11 | Isolation and complete genome sequence of a novel virulent mycobacteriophage, CASbig显示文摘Dear Editor,Bacteriophages are powerful tools for investigating and manipulating their hosts(Fernandes et al.,2014).This holds particularly true for mycobacteriophages,which have facilitated the development of mycobacterial genetic systems and have generated tools for the | Tieshan Teng Junping Yu Hang Yang Hongping Wei | 2015 | Virologica Sinica2015，30，1： | 1 |
| 12 | MicroRNA-15b regulates cell cycle progression by targeting cyclins in glioma cells显示文摘 | Hongping Xia Yanting Qi Samuel S. Ng Xiaona Chen Shen Chen Marong Fang Dan Li Yu Zhao Ruiguang Ge Guo Li Yangchao Chen Ming-Liang He Hsiang-fu Kung Lihui Lai Marie C. Lin | 2009 | Biochemical and Biophysical Research Communications2009，，2： | 1 |
| 13 | Experi- mental study on monitoring steel beam local corrosion based on EMI technique 显示文摘 | Yang Jingwen Zhu Hongping Yu Jing | 2013 | Applied Mechanics and Materials2013，273，： | 1 |
| 14 | Existence of Separate Domains in Lysin PlyG for Recognizing Bacillus anthracis Spores and Vegetative Cells显示文摘 | Hang Yang Dian-Bing Wang Qiuhua Dong Zhiping Zhang Zongqiang Cui Jiaoyu Deng Junping Yu Xian-en Zhang Hongping Wei | 2012 | Antimicrobial Agents and Chemotherapy2012，，10： | 1 |
| 15 | Abnormal gonad development in Kit^(W-2Bao) mice caused by a Kit gene missense mutation显示文摘KitW-2Bao mice are single-gene autosomal dominant mutation mice with a B6 background that were bred in our laboratory. Heterozygotes had morphological characteristics including albinism of the abdomen, extremities, and tail, whereas the homozygotes had albinism of the body, black eyes, and infertility. The homozygous mutants showed small, structurally abnormal gonads, and lacked germ cells. Heterozygous male mice lacked germ cells in some contorted seminiferous tubules. This mutation has been mapped at 43.8 cM from the centromere in chromosome 5 by linkage analysis and Kit has been identified as the candidate gene. After Kit full-length mRNA amplification, it was found that a G to T conversion at position 1228 in the ORF changed the 410th amino acid from V to F. This amino acid change could affect the protein’s secondary structure. Heterozygous mutant mice were intercrossed and homozygous mutant mice were bred and genotyped. We found that no primordial germ cells (PGCs) appeared in the urogenital ridge area at fetus day 11.5 in the homozygotes. The number of PGCs also significantly decreased in heterozygotes. At fetus day 15.5, the differentiation of the testis tubule structure was unclear; as well, they contained no spermatogonia. Female homozygotes contained no primordial follicles in the ovary. The numbers of PGCs and primordial follicles were significantly decreased in heterozygous mice. W-2Bao is the only mutated site in the extracellular 4th Ig-like domain and this mutant mouse model provides new material for the study of the mechanism of reproductive system development. | WU BaoJin YIN LiJing LU ZhengLan YIN YuShu YANG WeiWei YANG Rong KANG XiaoDong LIU GuiJie YIN HongPing YU LiPing Gu MeiEr WU PeiLin | 2010 | Chinese Science Bulletin2010，55，36： | 1 |
| 16 | Physical activation of diatomite-templated carbons and its effect on the adsorption of methylene blue (MB)显示文摘 | Dong Liu Weiwei Yuan Peng Yuan Wenbin Yu Daoyong Tan Hongmei Liu Hongping He | 2013 | Applied Surface Science2013，，： | 1 |
| 17 | Schaftoside inhibits 3CL^(pro)and PL^(pro)of SARS-CoV-2 virus and regulates immune response and inflammation of host cells for the treatment of COVID-19显示文摘It is an urgent demand worldwide to control the coronavirus disease 2019(COVID-19)pandemic caused by the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus.The 3-chymotrypsin-like protease(3CL^(pro))and papain-like protease(PL^(pro))are key targets to discover SARS-CoV-2 inhibitors.After screening 12 Chinese herbal medicines and 125 compounds from licorice,we found that a popular natural product schaftoside inhibited 3CL^(pro)and PL^(pro)with IC_(50)values of 1.73±0.22 and 3.91±0.19μmol/L,respectively,and inhibited SARS CoV-2 virus in Vero E6 cells with EC_(50)of 11.83±3. 23μmol/L. Hydrogen-deuterium exchange mass spectrometry analysis, quantum mechanics/molecular mechanics calculations, together with site-directed mutagenesis indicated the antiviral activities of schaftoside were related with non-covalent interactions with H41, G143 and R188 of3CLpro, and K157, E167 and A246 of PLpro. Moreover, proteomics analysis and cytokine assay revealed that schaftoside also regulated immune response and inflammation of the host cells. The antiinflammatory activities of schaftoside were confirmed on lipopolysaccharide-induced acute lung injury mice. Schaftoside showed good safety and pharmacokinetic property, and could be a promising drug candidate for the prevention and treatment of COVID-19. | Yang Yi Meng Zhang Heng Xue Rong Yu Yang-Oujie Bao Yi Kuang Yue Chai Wen Ma Jing Wang Xiaomeng Shi Wenzhe Li Wei Hong Junhua Li Elishiba Muturi Hongping Wei Joachim Wlodarz Szczepan Roszak Xue Qiao Hang Yang Min Ye | 2022 | Acta Pharmaceutica Sinica B2022，12，11： | 0 |
| 18 | Physically Activated Diatomite-templated Carbon with High Porosity for the Adsorption of Methylene Blue显示文摘Porous carbons are extensively applied in gas separation, water purification, catalytic reaction, and electrochemical processing, attributing to their high specific surface area, large pore volume, chemical inertness, and good mechanical and thermal stability. The templating method is widely used to synthesize porous carbons with the controlled pore structure. Among them, preparation of diatomite-templated carbons attracts increasing attention because the obtained carbon has unique developed macropores and exhibits the promising application in adsorption and support of large-sized molecules. Macroporous diatomite-templated carbons are prepared by using additive or inherent solid acid sites of diatomite as the catalyst. The obtained carbons showed tubular and pillared macroporous structures, and had a few mesopores and micropores. However, the carbons possessed the small specific surface area and micropore volume, and thus showed the low adsorption capacity of small-sized molecules, such as methylene blue (MB). In this case, enhancement of porosity, especially microporosity, is necessary. | LIU Dong YUAN Weiwei YUAN Peng YU Wenbin TAN Daoyong LIU Hongmei HE Hongping | 2013 | 矿物学报2013，33，S1： | 0 |
| 19 | ACE2-Targeting antibody suppresses SARS-CoY-2 Omicron and Delta variants显示文摘Dear Editor,The pandemic of COVID-19 continues worldwide with many variants arising,especially SARS-CoV-2 Delta(B.1.617.2)and Omicron(B.1.1.529)variants of concern(VOCs).According to WHO,Omicron has spread in almost all the countries with a doubling time less than Delta VOC. | Jianxia Ou Yanan Zhang Yongmei Wang Zherui Zhang Hongping Wei Junping Yu Qi Wang Guifeng Wang Bo Zhang Chunhe Wang | 2022 | Signal Transduction and Targeted Therapy2022，7，3： | 0 |
| 20 | Correction to: Rapid Acquisition of High-Quality SARS-CoV-2 Genome via Amplicon-Oxford Nanopore Sequencing显示文摘Correction to:Virologica Sinica(2021)36:901-912 http://gffzzd3cc09b8251d45dfsbbuckqb6vc596vn6.ffgz.tsg.suse.edu.cn/10.1007/sl 2250-021-00378-8 The original version of article contains errors in Figure 4,Supplementary Figure S2,Supplementary Table S2,and the corresponding description in part 3 of“Results”section. | Yi Yan Ke Wu Jun Chen Haizhou Liu Yi Huang Yong Zhang Jin Xiong Weipeng Quan Xin Wu Yu Liang Kunlun He Zhilong Jia Depeng Wang Di Liu Hongping Wei Jianjun Chen | 2021 | Virologica Sinica2021，36，6： | 0 |
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