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| 1 | Enhanced expression of interleukin-18 in serum and pancreas of patients with chronic pancreatitis显示文摘AIM: To investigate interleukin-18 (IL-18) in patients with chronic panreatitis (CP). METHODS: We studied 29 patients with CP and 30 healthy controls. Peripheral blood mononuclear cells (PBMC) were isolated and incubated with 50 mmol/L ethanol, lipopolysaccharide (LPS) (doses 25 g/L, 250 g/L, 2500 g/L) and both agents for 24 h. Levels of IL-18 in the supernatants, and levels of IL-18, IL-12, interferon (IFN)-γ and soluble CD14 in the serum were analysed by ELISA technique. Expression of IL-18 in PBMC was investigated by reverse-transcription (RT)-PCR. IL-18 protein levels in CP tissue and in normal pancreas were studied by ELISA technique. IL-18 levels in PBMC and pancreatic tissue were determined by Westernblot. Im-munohistochemistry for pancreatic IL-18 expression was performed. RESULTS: In patients, IL-18 serum levels were signifi-cantly enhanced by 76% (mean: 289.9 ± 167.7 ng/L) compared with controls (mean: 165.2 ± 43.6 ng/L; P < 0.0005). IL-12 levels were enhanced by 25% in patients (18.3 ± 7.3 ng/L) compared with controls (14.7 ± 6.8ng/L, P = 0.0576) although not reaching the statistical significance. IFN-γ and soluble CD14 levels were not in-creased. In vitro, LPS stimulated significantly and dose-dependently IL-18 secretion from PBMC. Incubation with ethanol reduced LPS-stimulated IL-18 secretion by about 50%. The mRNA expression of IL-18 in PBMC and the response of PBMC to ethanol and LPS was similar in CP patients and controls. In PBMC, no significant differences in IL-18 protein levels were detected between patients and controls. IL-18 protein levels were increased in CP tissues compared to normal pancreatic tissues. IL-18 was expressed by pancreatic acinar cells and by infiltrating inflammatory cells within the pancreas. CONCLUSION: IL-18 originates from the chronically inflammed pancreas and appears to be involved in the fibrotic destruction of the organ. | Alexander Schneider Stephan L Haas Ralf Hildenbrand Sren Siegmund Iris Reinhard Helmut Nakovics Manfred V Singer Peter Feick | 2006 | World Journal of Gastroenterology2006，12，40： | 4 |
| 2 | European experts consensus statement on cystic tumours of the pancreas显示文摘 | Marco Del Chiaro Caroline Verbeke Roberto Salvia Gunter Kl?ppel Jens Werner Colin McKay Helmut Friess Riccardo Manfredi Eric Van Cutsem Matthias L?hr Ralf Segersv?rd | 2013 | Digestive and Liver Disease2013，，： | 3 |
| 3 | Confocal laser endomicroscopy for the differential diagnosis of ulcerative colitis and Crohn’s disease: a pilot study显示文摘 | Gian Tontini Jonas Mudter Michael Vieth Raja Atreya Claudia Günther Yurdagül Zopf Dane Wildner Ralf Kiesslich Maurizio Vecchi Markus Neurath Helmut Neumann | 2014 | Endoscopy2014，，： | 2 |
| 4 | Pancreatic Enzyme Replacement Therapy in Patients With Exocrine Pancreatic Insufficiency Due to Chronic Pancreatitis: A 1-Year Disease Management Study on Symptom Control and Quality of Life显示文摘 | Jan G. D’Haese Güralp O. Ceyhan Ihsan Ekin Demir Peter Layer Waldemar Uhl Matthias L?hr Reinhard Rychlik Konstantinos Pirilis York Z?llner Birgit Gradl Douglas Foerster Julia M?bius Friederike Henniges Helmut Friess | 2014 | Pancreas2014，，： | 2 |
| 5 | The cause of land use and land cover change: moving beyond the myths显示文摘 | Eric F L Turner B L Helmut J G | 2001 | Global Environmental Change2001，11，4： | 1 |
| 6 | Social Origins of Civil Society: Explaining the Nonprofit Sector Cross- Nationally显示文摘 | SALAMON L M HELMUT K A | 1998 | Voluntas1998，9，3： | 1 |
| 7 | Magnetic induction tomography: hardware for multi-frequency measurements in biological tissues 显示文摘 | SCHARFETTER H HELMUT K L JAVIER R | 2001 | Physiol Meas2001，22，1： | 1 |
| 8 | The cause of land-use and land-cover change: moving beyond the myths显示文摘 | Eric F Lam bin TurnerB L Helmut J | 2001 | Global Environmental Change2001，11，： | 1 |
| 9 | Unstiffened steel plate shear wall performance under cyclic loading显示文摘 | ADAM S L HELMUT G L P | 2000 | Journal of Structural Engineering2000，126，4： | 1 |
| 10 | Registration without ICP 显示文摘 | Helmut P Stdfan L Michael H | 2004 | Computer Vision and Image Understanding2004，95，： | 1 |
| 11 | Infraredspectroscopic investigations of selective catalytic reduction catalysts poisoned with arsenic oxide显示文摘 | FRIEDERIKE C L HELMUT S HELMUT K | 1996 | Appl Catal B1996，8，24： | 1 |
| 12 | A challenge for plastic surgery 显示文摘 | Helmut F Wolfgang L | 2008 | Nasal Reconstruction2008，105，43： | 1 |
| 13 | An X-ray photo electron spectroscopy study of oxides of arsenic supported on Ti O2显示文摘 | FRIEDERIKE C L HELMUT S HELMUT K | 1991 | J Electron Spectrosc1991，57，30： | 1 |
| 14 | Water relationships and incorporation 14C of assimilates in tubers of potato plants differing in potassium nutrition显示文摘 | HELMUT B HANS H MEINOLF L | 1983 | Plant Physiol1983，73，： | 1 |
| 15 | European experts consensus statement on cystic tumours of the pancreas显示文摘 | Marco Del Chiaro Caroline Verbeke Roberto Salvia Gunter Kl?ppel Jens Werner Colin McKay Helmut Friess Riccardo Manfredi Eric Van Cutsem Matthias L?hr Ralf Segersv?rd | 2013 | Digestive and Liver Disease2013，，： | 1 |
| 16 | The cause of land-use and land-cover change:moving beyond the myths显示文摘 | Eric F Lambin B L Turner Helmut J Geist | 2001 | Global Environmental Change2001，11，4： | 1 |
| 17 | lnvestigating Stability and Linearity of a German M 1 Money Demand Function显示文摘 | Helmut L ti tkepohl Timo Teaavirta J u rgen Wolters | 1999 | Jourhal of Applied Econometrics1999，，14： | 1 |
| 18 | The Cause of Land Use and Land Cover Change: Moving Beyond the Myths 显示文摘 | Eric F Lambin B L Turner Helmut J Geist | 2001 | Global Environmental Change2001，，： | 1 |
| 19 | Synthesis of Spirorenone-a Novel Highly Active Aldosterone Antagonist显示文摘 | Dieter B Helmut H Henry L | 1982 | Angew Chew1982，94，： | 1 |
| 20 | Global environment change:moving beyond the myths显示文摘 | Eric F Lambin Turner B L Helmut J Geist | 2001 | Global Environment Change2001，11，： | 1 |
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