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| 1 | In situ tumor vaccination with adenovirus vectors encoding measles virus fusogenic membrane proteins and cytokines显示文摘AIM: To evaluate whether intratumoral expression of measles virus fusogenic membrane glycoproteins H and F (MV-FMG), encoded by an adenovirus vector Ad.MV-H/ F, alone or in combination with local coexpression of cytokines (IL-2, IL-12, IL-18, IL-21 or GM-CSF), can serve as a platform for inducing tumor-specifi c immune responses in colon cancer. METHODS: We used confocal laser scanning microscopy and flow cytometry to analyze cell-cell fusion after expression of MV-FMG by dye colocalization. In a syngeneic bilateral subcutaneous MC38 and Colon26 colon cancer model in C57BL/6 and BALB/c mice, we assessed the effect on both the directly vector-treated tumor as well as the contralateral, not directly vector- treated tumor. We assessed the induction of a tumor- specific cytotoxic T lymphocyte (CTL) response with a lactate dehydrogenase (LDH) release assay. RESULTS: We demonstrated in vitro that transduction of MC38 and Colon26 cells with Ad.MV-H/F resulted in dye colocalization, indicative of cell-cell fusion. In addition, in the syngeneic bilateral tumor model we demonstrated a signifi cant regression of the directly vector-inoculated tumor upon intratumoral expression of MV-FMG alone or in combination with the tested cytokines. We observed the highest anti-neoplastic efficacy with MV-FMG and IL-21 coexpression. The degree of tumor regression of the not directly vector-treated tumor correlated with the anti-neoplastic response of the directly vector-treated tumor. This regression was mediated by a tumor-specifi c CTL response. CONCLUSION: Our data indicate that intratumoral expression of measles virus fusogenic membraneglycoproteins is a promising tool both for direct tumor treatment as well as for tumor vaccination approaches that can be further enhanced by cytokine coexpression. | Dennis Hoffmann Wibke Bayer Oliver Wildner | 2007 | World Journal of Gastroenterology2007，13，22： | 4 |
| 2 | Confocal laser endomicroscopy for the differential diagnosis of ulcerative colitis and Crohn’s disease: a pilot study显示文摘 | Gian Tontini Jonas Mudter Michael Vieth Raja Atreya Claudia Günther Yurdagül Zopf Dane Wildner Ralf Kiesslich Maurizio Vecchi Markus Neurath Helmut Neumann | 2014 | Endoscopy2014，，： | 2 |
| 3 | Therapy of peritoneal carcinomatosis from colon cancer with oncolytic adenoviruses 显示文摘 | Wildner O Morris JC | 2000 | J Gene Med2000，2，： | 1 |
| 4 | Comparison of HSV-1 thymidine kinase-dependent and -independent inhibition of replication-competent adenoviral vectors by a panel of drugs显示文摘 | Wildner O Hoffmann D Jogler C | | 0，，10： | 1 |
| 5 | Interleukin-1 be- ta and tumor necrosis factor alpha inhibit migration activity of chondrogenic progenitor cells from non-fibrillated osteoarthrit- ic cartilage显示文摘 | JOOS H WILDNER A HOGREFE C | 2013 | Arthritis Res Therapy2013，15，5： | 1 |
| 6 | Adenoviral vectors capable of replication improve the efficacy of HSVtk/GCV suicide gene therapy of cancer显示文摘 | Wildner O Morris JC Vahanian NN | 1999 | Gene Ther1999，6，： | 1 |
| 7 | Telomerase activity levels in the surgical margin and tumour distant tissue of the squamous cell carcinoma of the head-and-neck显示文摘 | Fabricius E M Gur U Wildner G P | 2002 | Anal Cell Pathol2002，24，： | 1 |
| 8 | Gene transfer of heat-shock protein 70 reduces infarct size in vivo after ischemia/reperfusion in the rabbit heart显示文摘 | Okubo S Wildner O Shah MR | 2001 | Circulation2001，103，6： | 1 |
| 9 | Therapy of colon cancer withoncolytic adenovirus is enhanced by the addition of herpes simplexvims - thymidine kinase 显示文摘 | Wildner 0 Blaese RM Morris JC | 1999 | Cancer Res1999，59，2： | 1 |
| 10 | Characterization of virus isolates by particle-associated nucleic acid PCR 显示文摘 | Stang A Korn K Wildner O | 2005 | J Clin Microbiol2005，43，2： | 1 |
| 11 | Comparison of the corneal thickness as measured by Visante anterior segment OCT versus ultrasound technique显示文摘 | Wildner K Müller M Dawczynski J | | 0，，： | 1 |
| 12 | Retinoblastoma显示文摘 | Villegas VM Hess DJ Wildner A | 2013 | Curr Opin Ophthalmol2013，24，6： | 1 |
| 13 | Adenoviral vectors capable of replication improve the efficacy of HSVtk/GCV suicide gene therapy of cancer显示文摘 | Wildner O Morris JC Vahanian NN | 1999 | Gene Ther1999，6，1： | 1 |
| 14 | Interleukin - 1 beta and tumor necrosis factor alpha inhibit migration activity of chondrogenic progeni- tor cells from non -fibrillated osteoarthritic cartilage显示文摘 | Joos H Wildner A Hogrefe C | 2013 | Arthritis Res Ther2013，15，5： | 1 |
| 15 | Intellectual outcome,motor skills and BMI of children with congenital hypothyroidism:a population-based study显示文摘 | Arenz S Nennstiel-Ratzel U Wildner M | 2008 | Acta Paediatr2008，97，4： | 1 |
| 16 | Autoimmune uveitis and antigenic mimicry of environmental antigens 显示文摘 | Wildner G Diedrichs-Mohring M | 2004 | Autoimmun Rev2004，3，5： | 1 |
| 17 | Retino- bl astoma 显示文摘 | Villegas VM Hess D J Wildner A Gold AS Murray TG | 2013 | Curt Opin Ophthalmol2013，24，6： | 1 |
| 18 | Gene transfer of heat-shock protein 70 reduces infarct size in vivo after ischemia/reperfusion in the rabbit heart显示文摘 | Okubo S Wildner O Shah MR | 2001 | Circulation2001，103，6： | 1 |
| 19 | Synergy between the herpes simplex virus tk/ganciclovir prodrug suicide system and the topoisomerase 1 inhibitor topotecan 显示文摘 | Wildner O Blaese RM Morris JC | 1999 | Hum Gene Ther1999，10，16： | 1 |
| 20 | Characterization of virus isolates by particle-associated nucleic acid PCR显示文摘 | Stang A Korn K Wildner O | 2005 | Clin Mi- erobiol2005，43，2： | 1 |
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