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| 1 | A High-Density SNP Genotyping Array for Rice Biology and Molecular Breeding显示文摘一个高密度的单个核苷酸多型性(SNP ) 数组为遗传学者和分子的 breeders.With 是极其重要的 genomic 的巨大的数量的累积重新定序为精确 SNP 察觉的数据和可得到的技术,设计高密度、高质量的米饭 SNP 数组是可能的。这里,我们报导一个高密度的 riceSNP 数组和它的实用程序的开发。SNP 探针被屏蔽超过 10 设计从变化和一个数组说出 RiceSNP50 的 801 米饭的 re-sequencingdata 提取的 000 000 SNP loci 在 Illumina Infinium 站台上被生产。数组 contained51 478 个均匀地分布式的标记,其 68% 个在遗传因子的区域以内。有 parent/F1 relationshipswere 的几百米饭植物过去常为精确 SNP 打电话产生一个高质量的簇文件。应用程序测试证明这穿有的 highgenotyping 精确性,并且能被用于不同目的。例如,有好分辨率的精英米饭变化 wasclustered 的一个核心集合。染色体宽的协会研究(GWAS ) 分析正确地识别了描绘的 QTL.Further,这个数组成功地为变化确认和特点基因渗入被使用。作为一个精确 high-throughputgenotyping 工具, RiceSNP50 将在两功能的 genomics 学习和分子的 breeding.Key 词起一个重要作用: | Haodong Chen Weibo Xie Hang He Huihui Yu Wei Chen Jing Li Renbo Yu Yue Yao Wenhui Zhang Yuqing He Xiaoyan Tang Fasong Zhou Xing Wang Deng Qifa Zhang | 2014 | Molecular Plant2014，7，3： | 38 |
| 2 | Olive leaf extract inhibits lead poisoning-induced brain injury显示文摘Olive leaves have an antioxidant capacity,and olive leaf extract can protect the blood,spleen and hippocampus in lead-poisoned mice.However,little is known about the effects of olive leaf extract on lead-induced brain injury.This study was designed to determine whether olive leaf extract can inhibit lead-induced brain injury,and whether this effect is associated with antioxidant capacity.First,we established a mouse model of lead poisoning by continuous intragastric administration of lead acetate for 30 days.Two hours after successful model establishment,lead-poisoned mice were given olive leaf extract at doses of 250,500 or 1000 mg/kg daily by intragastric administration for 50 days.Under the transmission electron microscope,olive leaf extract attenuated neuronal and capillary injury and reduced damage to organelles and the matrix around the capillaries in the frontal lobe of the cerebral cortex in the lead-poisoned mice.Olive leaf extract at a dose of 1000 mg/kg had the greatest protective effect.Spectrophotometry showed that olive leaf extract significantly increased the activities of superoxide dismutase,catalase,alkaline phosphatase and acid phosphatase,while it reduced malondialdehyde content,in a dose-dependent manner.Furthermore,immunohistochemical staining revealed that olive leaf extract dose-dependently decreased Bax protein expression in the cerebral cortex of lead-poisoned mice.Our findings indicate that olive leaf extract can inhibit lead-induced brain injury by increasing antioxidant capacity and reducing apoptosis. | Yu Wang Shengqing Wang Wenhui Cui Jiujun He Zhenfu Wang Xiaolu Yang | 2013 | Neural Regeneration Research2013，8，22： | 21 |
| 3 | ROS-responsive drug delivery systems for biomedical applications显示文摘In the field of biomedicine, stimuli-responsive drug delivery systems(DDSs) have become increasingly popular due to their site-specific release ability in response to a certain physiological stimulus, which may result in both enhanced treatment outcome and reduced side effects. Reactive oxygen species(ROS) are the unavoidable consequence of cell oxidative metabolism. ROS play a crucial part in regulating biological and physiological processes,whereas excessive intracellular ROS usually lead to the oxidation stress which has implications in several typical diseases such as cancer, inflammation and atherosclerosis. Therefore,ROS-responsive DDSs have elicited widespread popularity for their promising applications in a series of biomedical research because the payload is only released in targeted cells or tissues that overproduce ROS. According to the design of ROS-responsive DDSs, the main release mechanisms of therapeutic agents can be ascribed to ROS-induced carrier solubility change, ROS-induced carrier cleavage or ROS-induced prodrug linker cleavage. This review summarized the latest development and novel design of ROS-responsive DDSs and discussed their design concepts and the applications in the biomedical field. | Wenhui Tao Zhonggui He | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，2： | 12 |
| 4 | Insight-HXMT observations of the first binary neutron star merger GW170817显示文摘Finding the electromagnetic(EM) counterpart of binary compact star merger, especially the binary neutron star(BNS) merger,is critically important for gravitational wave(GW) astronomy, cosmology and fundamental physics. On Aug. 17, 2017,Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart,GRB 170817 A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope(HE) onboard Insight-HXMT(Hard X-ray Modulation Telescope) is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart(SSS17 a/AT2017 gfo) with very large collection area(~1000 cm^2) and microsecond time resolution in 0.2-5 MeV. In addition,Insight-HXMT quickly implemented a Target of Opportunity(ToO) observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy(0.2-5 MeV) radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817 A. Meanwhile,Insight-HXMT/HE provides one of the most stringent constraints(~10^(-7) to 10^(-6) erg/cm^2/s) for both GRB170817 A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event. | TiPei Li ShaoLin Xiong ShuangNan Zhang FangJun Lu LiMing Song XueLei Cao Zhi Chang Gang Chen Li Chen TianXiang Chen Yong Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong YuanYuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao CongZhan Liu GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge OU JinLu Qu Na Sai Liang Sun Yin Tan Lian Tao WenHui Tao YouLi Tuo GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BoBing WU Mei Wu GuangCheng Xiao He Xu YuPeng Xu LinLi Yan JiaWei Yang Sheng Yang YanJi Yang AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang Shu Zhang Tong Zhang Wei Zhang WanChang Zhang WenZhao Zhang Yi Zhang Yue Zhang YiFei Zhang YongJie Zhang Zhao Zhang ZiLiang Zhang HaiSheng Zhao JianLing Zhao XiaoFan Zhao ShiJie Zheng Yue Zhu YuXuan Zhu ChangLin Zou | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，3： | 12 |
| 5 | Global correlation for strontium isotope curve in the Late Cretaceous of Tibet and dating marine sediments显示文摘87Sr/86Sr ratios of marine carbonate samples collected from a sedimentary section of the Late Cretaceous in the south of Tibet were measured. Based on the absence of cathodoluminescence and a very low Mn/Sr ratio (average 0.06) of the samples, it is thought that they contain information on the original seawater strontium isotope composition. The strontium isotope evolution curve of the Late Cretaceous in Tibet we established here, is consistent with other coeval curves from Europe, North America and Antarctica, supports the notion that the strontium isotope composition of seawater is governed by global events, which provides a new approach for the inter-continental and inter-basinal correlations of Late Cretaceous in the area and is a complementarity for biostratigraphy. In addition, we attempt to determine the age of the boundaries for Campanian/Santonian and Maastrichtian/Campanian by 87Sr/86Sr ratios for Gamba section in southern Tibet. The two boundaries are located in the thickness of 217 m (83.5 Ma) and 291 m (71.3 Ma), respectively. | HUANG Sijing SHI He SHEN Licheng ZHANG Meng WU Wenhui | 2005 | Science China Earth Sciences2005，48，2： | 11 |
| 6 | Impact of urban expansion on meteorological observation data and overestimation to regional air temperature in China显示文摘自从改革的实现并且在 1970 年代末在中国开创政策,一些气象学的车站由于城市的扩大消极地进入了城市。在在车站附近的表面和开发环境的变化影响了空气温度的观察。当来自城市的车站的观察数据在公民或地区性的规模空气温度数据集的插值被使用时,他们能导致地区性的空气温度的 overestimation 和对温暖的不精密的评价。在这研究,越过中国包围 756 个气象学的车站的内在的表面在很多时间间隔上基于遥感图象被识别区分进入了城市的农村车站。在把观察数据从被城市的扩大影响了的这些车站移开以后,然后,背景空气温度的数据集被插入内推产生从仍然保持农村的观察数据驻扎。吝啬的城市的热岛效果紧张自从 1970 被从城市的车站把原来的观察记录与背景空气作比较估计,温度插入内推。结果证明那城市的热岛效果确实由于城市的扩大发生,与在比在另外的季节的冬季的更高的紧张。然后,地区性的空气温度的 overestimation 被比较分别地由所有车站和农村车站被插入内推的空气温度的二种格子数据集评估观察数据。空间地, overestimation 比在中国的中央部分在东方中国是相对更高的;然而,两个区域比的展出高得多的效果在西方的中国被观察。我们在最后 40 年里结束了那在中国的温度增加了大约 1.58 的平均数,降水,浅地面温度和 NDVI,哪个 3 吗?? | SHAO Quanqin SUN Chaoyang LIU Jiyuan HE Jianfeng KUANG wenhui TAO Fulu | 2011 | Journal of Geographical Sciences2011，21，6： | 10 |
| 7 | Procalcitonin could be a reliable marker in differential diagnosis of post-implantation syndrome and infection after percutaneous endovascular aortic repair显示文摘 | Xue Ling Luo Songyuan Luo Jianfang Liu Zhen Gu Mengnan Kang Huiyuan Yang Fan Nie Bingrong Liu Yuan Huang Wenhui Xie Nianjin He Pengcheng Dong Haojian Ni Zhonghan Fan Ruixin Chen Jiyan | 2014 | Chinese Medical Journal2014，，14： | 8 |
| 8 | High-Resolution Genome-wide Association Study Identifies Genomic Regions and Candidate Genes for Important Agronomic Traits in Wheat显示文摘Wheat(Triticum aestivum)is a major staple food crop worldwide.Genetic dissection of important agronomic traits is essential for continuous improvement of wheat yield to meet the demand of the world's growing population.We conducted a large-scale genome-wide association study(GWAS)using a panel of 768 wheat cultivars that were genotyped with 327609 single-nucleotide polymorphisms generated by genotyping-by-sequencing and detected 395 quantitative trait loci(QTLs)for 12 traits under 7 environments.Among them,273 QTLs were delimited to≤1.0-Mb intervals and 7 of them are either known genes(Rht-D,Vrn-B1,and Vrn-D1)that have been cloned or known QTLs(TaGA2ox8,APO1,TaSus1-7B,and Rht12)that were previously mapped.Eight putative candidate genes were identified for three QTLs that enhance spike seed setting and grain size using gene expression data and were validated in three bi-parental populations.Protein sequence analysis identified 33 putative wheat orthologs that have high identity with rice genes in QTLs affecting similar traits.Large r^2 values for additive effects observed among the QTLs for most traits indicated that the phenotypes of these identified QTLs were highly predictable.Results from this study demonstrated that significantly increasing GWAS population size and marker density greatly improves detection and identification of candidate genes underlying a QTL,solidifying the foundation for large-scale QTL fine mapping,candidate gene validation,and developing functional markers for genomics-based breeding in wheat. | Yunlong Pang Chunxia Liu Danfeng Wang Paul St.Amand Amy Bernardo Wenhui Li Fang He Linzhi Li Liming Wang Xiufang Yuan Lei Dong Yu Su Huirui Zhang Meng Zhao Yunlong Liangi Hongze Jia Xitong Shen Yue Lu Hongming Jiang Yuye Wu Anfei Li Honggang Wang Lingrang Kong Guihua Bai Shubing Liu | 2020 | Molecular Plant2020，13，9： | 8 |
| 9 | Physical location of rice Gm-6,Pi-5(t) genes in O.officinalis with BAC-FISH显示文摘A fluorescence in situ hybridization (FISH) procedure was adopted to physically map two rice BAC clones 24E21 and 4F22 linked to Gm-6 and Pi-5(t) in O. offi-cinalis. FISH results showed that the two BAC clones were located at 4L. The percentage distance from the centromere to the hybridization sites was 72 ± 2.62 for 24E21 and 54± 5.43 for 4F22, the detection rates were 52.70% and 61.2%. The results obtained from the BAC and plasmid clones, RG214 and RZ565 of cultivated rice and O. officinalis were the same. This suggested that the markers, RG214 and RZ565 of cultivated rice and O. officinalis were in the same BAC clones. The homologous sequences of Gm-6 and Pi-5(t) in O. officinalis were positions that signals existed on the 4L. Many signals were observed when no Cot-1 DNA blocked. This also showed that repetitive sequences were some ho-molgous between cultivated rice and O. officinalis. The identification of chromosome 4 of O. officinalis is based on Jena et al. (1994). In our study, we discussed | QIN Rui WEI Wenhui JIN Weiwei HE Guangcun NING Shunbin YU Shunwu SONG Yunchun | 2001 | Chinese Science Bulletin2001，46，8： | 6 |
| 10 | The zinc transporter Slc39a5 controls glucose sensing and insulin secretion in pancreatic β-cells via Sirt1- and Pgc-1α-mediated regulation of Glut2显示文摘Zinc levels are high in pancreatic β-cells, and zinc is involved in the synthesis, processing and secretion of insulin in these cells. However, precisely how cellular zinc homeostasis is regulated in pancreatic β-cells is poorly understood. By screening the expression of 14 Slc39a metal importer family member genes, we found that the zinc transporter Slc39a5 is significantly downregulated in pancreatic β-cells in diabetic db/db mice, obese ob/ob mice and high-fat diet-fed mice. Moreover,β-cell-specific Slc39a5 knockout mice have impaired insulin secretion. In addition, Slc39a5-deficient pancreatic islets have reduced glucose tolerance accompanied by reduced expression of Pgc-1α and its downstream target gene Glut2. The down-regulation of Glut2 in Slc39a5-deficient islets was rescued using agonists of Sirt1, Pgc-1α and Ppar-γ. At the mechanistic level, we found that Slc39a5-mediated zinc influx induces Glut2 expression via Sirt1-mediated Pgc-1α activation. These findings suggest that Slc39a5 may serve as a possible therapeutic target for diabetes-related conditions. | Xinhui Wang Hong Gao Wenhui Wu Enjun Xie Yingying Yu Xuyan He Jin Li Wanru Zheng Xudong Wang Xizhi Cao Zhuoxian Meng Ligong Chen Junxia Min Fudi Wang | 2019 | Protein & Cell2019，10，6： | 6 |
| 11 | A thinned lithosphere beneath coastal area of southeastern China as evidenced by seismic receiver functions显示文摘During Mesozoic to Cenozoic time, the large-scale tectono-magmatism had strongly modified the lithosphere beneath the southeastern continent of China, leaving the present-day lithosphere as a new one evolving from the ancient lithosphere that was largely removed and replaced. But this model proposed from geochemical and petrological research is urgently in need of support from seismic observational evidence. In this paper, based on the dataset recorded by the dense stations of two NE oriented broadband seismic profiles deployed in the coastal area of southeastern China(SE China), both P-wave(P-RF) and S-wave(S-RF) receiver functions were isolated. We identified Pls phase converted from the Lithosphere-Asthenosphere Boundary(LAB) in P-RFs of individual stations. Migrated Pls phase indicated a depth of 60–70 km for LAB. Inversions/comparisons of P-RF(Pls phase) and S-RF(Slp phase) waveforms together with Ps and Sp imaging for the crust and upper mantle structure further confirmed this result. P-RF and S-RF migrated images exhibit that a flat LAB is positioned at the depth of 60–70 km spreading along the profile, whereas a distinct structural change of lithospheric base appears at the Min River estuary. Both Ps and PpPs migrated images of P-RFs present an abrupt Moho drop across the Min River fault from south to north, which is consistent with previous result obtained from deep seismic sounding. By taking into consideration other geological and geophysical features such as locally high anomalies of crustal Poisson's ratios and heat flow at the Min River estuary, we infer that the Min River fault penetrates down to the Moho and may, furthermore, interfere in the deeper lithospheric structure. | YE Zhuo LI QiuSheng GAO Rui ZHANG HongShuang HE RiZheng WANG HaiYan LI WenHui | 2014 | Science China Earth Sciences2014，57，11： | 6 |
| 12 | The IAA-and ABA-responsive transcription factor CgMYB58 upregulates lignin biosynthesis and triggers juice sac granulation in pummelo显示文摘In citrus,lignin overaccumulation in the juice sac results in granulation and an unpleasant fruit texture and taste.By integrating metabolic phenotyping and transcriptomic analyses,we found 702 differentially expressed genes(DEGs),including 24 transcription factors(TFs),to be significantly correlated with lignin content.CgMYB58 was further identified as a critical R2R3 MYB TF involved in lignin overaccumulation owing to its high transcript levels in Huanong Red-fleshed pummelo(HR,Citrus grandis)fruits.Transient expression of CgMYB58 led to an increase in the lignin content in the pummelo fruit mesocarp,whereas its stable overexpression significantly promoted lignin accumulation and upregulated 19 lignin biosynthetic genes.Among these genes,CgPAL1,CgPAL2,Cg4CL1,and CgC3H were directly modulated by CgMYB58 through interaction with their promoter regions.Moreover,we showed that juice sac granulation in pummelo fruits could be affected by indole-3-acetic acid(IAA)and abscisic acid(ABA)treatments.In HR pummelo,ABA significantly accelerated this granulation,whereas IAA effectively inhibited this process.Taken together,these results provide novel insight into the lignin accumulation mechanism in citrus fruits.We also revealed the theoretical basis via exogenous IAA application,which repressed the expression of CgMYB58 and its target genes,thus alleviating juice sac granulation in orchards. | Meiyan Shi Xiao Liu Haipeng Zhang Zhenyu He Hongbin Yang Jiajing Chen Jia Feng Wenhui Yang Youwu Jiang Jia-Long Yao Cecilia Hong Deng Juan Xu | 2020 | Horticulture Research2020，7，1： | 4 |
| 13 | Interferon as a Mucosal Adjuvant for an Influenza Vaccine in Pigs显示文摘Interferon,a natural protein that is produced by a variety of cells during viral infection,activates the transcription of multiple functional genes in cells,regulates synergy among various signaling pathways,and mediates many biological functions such as antiviral activity,immune regulation,and cell growth.However,clinical research on interferon in livestock is lacking.In this study,recombinant porcine interferon(PoIFNα)was used as an adjuvant,in combination with inactivated influenza virus,to vaccinate 6-week-old pigs via nasal infusion.The transcription of target genes was then monitored and the functions of PoIFNαwere determined with respect to the activation of mucosal immunity.We found that a combination of low-dose PoIFNαand in activated influenza virus could significantly up-regulate the expression of immunoregulatory cytokines such as IL-2,IL-1β,IFN-γ,IL-6,and IL-10 by real-time PCR,suggesting the induction of a strong mucosal innate immune response after administration.In addition,low-dose PoIFNαcan significant enhancing the transcription of genes encoding homing factors including CCR9 and CCR10(P<0.001),thereby resulting in the induction of higher levels of HA-specific antibodies(P<0.05),which can be determined by ELISA and IFA.Post-immunization challenges with H1N1 virus demonstrated that PoIFNot,combined with inactivated influenza virus,could alleviate clinical signs in pigs during the early stages of viral infection.These studies reveal low-dose PoIFNαas a potential mucosal adjuvant for influenza virus in pigs. | Lirong Liu Wenhui Fan He Zhang Shuang Zhang Liang Cui Meng Wang Xiaoyuan Bai Wenxian Yang Lei Sun Limin Yang Wenjun Liu Jing Li | 2019 | Virologica Sinica2019，34，3： | 3 |
| 14 | Optical super-resolution microscopy and its applications in nano-catalysis显示文摘常规光显微镜学的分辨率仅仅是 200 nm,它为许多应用程序正在变得越来越不足够。为了超过衍射,限制了分辨率,超级决定的显微镜学(SRM ) 被开发了完成一~十纳米的高分辨率。涉及 SRM 的技术能被分配进二个宽广范畴,也就是真的超级决定的技术和功能的超级决定的技术。在功能的超级决定的技术,随机的超级决定的显微镜学(SSRM ) 广泛地由于它的低开销,简单操作,和高分辨率被使用。在 SSRM 的原则进程是积累许多衍射有限 emitters 的坐标(例如,单个荧光灯分子) 在由本地化点的矩心的对象上散布函数(PSF ) ,然后重建用这些的对象的图象协调。什么时候衍射有限 emitters 由 nanoparticles 参加关于催化作用的催化反应,活动分发和运动信息能被 SSRM 获得。SSRM 被使用了并且在催化作用的几块地里展出了突出的优点,例如金属 nanoparticle 催化作用,分子的筛催化作用,和光催化。因为 SSRM 能在一 nanoparticle 以内解决催化活动,它答应加速新、更好的催化剂的发展和发现。这评论将在 nano 催化作用介绍简短介绍给 SRM,和对 SSRM 和它的应用的详细描述。 | Wenhui Wang Junnan Gu Ting He Yangbin Shen Shaobo Xi Lei Tian Feifei Li Haoyuan Li Liuming Yan Xiaochun Zhou | 2015 | Nano Research2015，8，2： | 3 |
| 15 | The self-assembled nanoparticle-based trimeric RBD mRNA vaccine elicits robust and durable protective immunity against SARS-CoV-2 in mice显示文摘As COVID-19 continues to spread rapidly worldwide and variants continue to emerge,the development and deployment of safe and effective vaccines are urgently needed.Here,we developed an mRNA vaccine based on the trimeric receptor-binding domain(RBD)of the SARS-CoV-2 spike(S)protein fused to ferritin-formed nanoparticles(TF-RBD).Compared to the trimeric form of the RBD mRNA vaccine(T-RBD),TF-RBD delivered intramuscularly elicited robust and durable humoral immunity as well as a Th 1-biased cellular response.After further challenge with live SARS-CoV-2,immunization with a two-shot low-dose regimen of TF-RBD provided adequate protection in hACE2-transduced mice.In addition,the mRNA template of TF-RBD was easily and quickly engineered into a variant vaccine to address SARS-CoV-2 mutations.The TF-RBD multivalent vaccine produced broad-spectrum neutralizing antibodies against Alpha(B.1.1.7)and Beta(B.1.351)variants.This mRNA vaccine based on the encoded self-assembled nanoparticle-based trimer RBD provides a referenee for the design of mRNA vaccines targeting SARS-CoV-2. | Wenqiang Sun Lihong He He Zhang Xiaodong Tian Zhihua Bai Lei Sun Limin Yang Xiaojuan Jia Yuhai Bi Tingrong Luo Gong Cheng Wenhui Fan Wenjun Liu Jing Li | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 3 |
| 16 | Landscape of the regulatory elements for lysine 2-hydroxyisobutyrylation pathway显示文摘短链的丰满的酸和他们的相应 acyl-CoAs 在新陈代谢的小径的十字路口坐并且在多样的细胞的过程起重要作用。他们也是为蛋白质 translational 以后离氨酸 acylation 修正的先锋。一个引人注目的例子是最新识别的离氨酸 2-hydroxyisobutyrylation (K 从 2-hydroxyisobutyrate 和 2-hydroxyisobutyryl-CoA 被导出的 hib ) 。Histone K hib 被显示了在 spermatogenic 房间与活跃基因表示被联系。然而,调整这条 translational 以后离氨酸 acylation 小径的关键元素仍然保持未知。这妨碍了这修正由施加它的生物功能的机制的描述。这里,我们在开始发育的酵母显示出那 Esa1p,它在人的相当或相同事物 Tip60 能增加 K hib 到在 vitro 并且在 vivo 的底层蛋白质。另外,我们作为主要的酶识别了 HDAC2 和 HDAC3 移开 K hib 。而且,我们报导第一 K 在哺乳动物的房间的 hib proteome,识别 6 548 K 1 725 底层蛋白质上的 hib 地点。我们的学习因此为 histone K hib 标记,和主要 K hib 蛋白质底层。这些结果不仅说明这条新离氨酸 acylation 小径的风景,而且为学习与这条小径联系的细胞的代谢物的多样的功能打开新大街。 | He Huang Zhouqing Luo Shankang Qi Jing Huang Peng Xu Xiuxuan Wang Li Gao Fangyi Li Jian Wang Wenhui Zhao Wei Gu Zhucheng Chen Lunzhi Dai Junbiao Dai Yingming Zhao | 2018 | Cell Research2018，28，1： | 2 |
| 17 | Antioxidant and ACE inhibitory activities of peptides prepared from adzuki bean by semi-solid enzymatic hydrolysis显示文摘In this study,adzuki bean peptides were prepared by semi-solid enzymatic hydrolysis(SEH)and their antioxidant and Angiotensin-I-converting enzyme(ACE)inhibitory activities and internal absorption were studied.By modifying different enzymatic hydrolysis times and enzyme additions,the optimal enzymatic hydrolysis condition for SEH could be obtained as adzuki bean powder:water=4:1(w/w),alcalase:neutrase=2:1(w/w),enzyme addition 3.75%(w/w),4 h enzymatic hydrolysis at 50℃ and pH=8.Within these hydrolysis conditions,the content of adzuki bean peptides was 11.37%,the OH−scavenging rate of 1 mg/mL peptides was 72.75%,the total antioxidant capacity was 0.88±0.02 mmol/L FeSO4,the ACE inhibition rate was 77.49%.In vivo absorption test proved that the peptides prepared by SEH were absorbed faster and exhibited higher antioxidant activity compared with liquid enzymatic hydrolysis.In conclusion,the process of SEH can improve not only the efficiency of hydrolysis,but also the antioxidant and ACE inhibitory activity as well as the internal absorption of adzuki bean peptides. | Mengdi Cao Wenhui Li He Li Jian Zhang Yu Liu Xinqi Liu | 2022 | Food Bioscience2022，47，3： | 2 |
| 18 | Effect of Zibushenjing Fang on skeletal development and brain tissue antioxidation in mice with kidney essence insufficiency: a mechanistic study显示文摘OBJECTIVE: To investigate the effects of Zibushenjing Fang (formula for tonifying the kidney essence) on skeletal development and brain tissue antioxidation in mice with kidney essence insufficiency. METHODS: Fifty male Kunming mice were randomly divided into five groups: normal group, model group, Jinkuishenqi Wan group, Zibushenjing Fang high dose group, and Zibushenjing Fang low dose group, with 10 mice in each group. The model of kidney essence insufficiency syndrome was established in all the mice except the normal group by using a cat to threaten the mice and by swimming until exhaustion daily which lasted about 21 days. Mice in the model group were administered 20 mL/ kg -1 · d -1 of normal saline intragastrically. The Jinkuishenqi Wan group was given 2.7 g/kg-1 · d-1 ofa solution of Jinkuishenqi Wan. The Zibushenjing Fang high dose group was given 20 g/kg -1 · d -1 and the Zibushenjing Fang low dose group was given 10 g/kg -1 · d -1 of a solution of Zibushenjing Fang.The general condition of all the groups was observed, including the quantity of food and water intake, swimming time, length of femur, and weight of the femur and musculus quadriceps femoris. The total superoxide dismutase (T-SOD), glutathione peroxi- dase (GSH-Px), and methane dicarboxylic aldehyde (MDA) activities in the brain tissues were detected. RESULTS: Zibushenjing Fang could improve the manifestation of kidney essence insufficiency, increase the quantity of food and water intake, swimming time, femur length, and femur and musculus quadriceps femoris weight. It could also regulate the activities of T-SOD, GSH-Px, and MDA in brain tissue. CONCLUSION: Zibushenjing Fang may play an important role in treating kidney essence insufficiency syndrome by promoting body development and improving brain tissue antioxidation. | Yuezhen He Junxiao Yao Shilin Shen Xiaojun Su Yuping Wang Li Li Wenhui Wang Jiping Shi | 2013 | Journal of Traditional Chinese Medicine2013，33，3： | 2 |
| 19 | Rapid and specific isolation of intact mitochondria from Arabidopsis leaves显示文摘Mitochondria carry out many essential metabolic processes, the dynamic of which impacts most aspects of cellular physiology(Balk and Leaver, 2001;Rose and Sheahan, 2012). Therefore, characterizing the real-time metabolism of mitochondria is of great biological significance. | Lilan Luo Yajun He Qian Xu Wenhui Lyu Jijun Yan Peiyong Xin Dahan Zhang Jinfang Chu Jiayang Li Hong Yu | 2020 | Journal of Genetics and Genomics2020，47，1： | 1 |
| 20 | H7N9 Influenza Viruses Are Transmissible in Ferrets by Respiratory Droplet显示文摘 | Qianyi Zhang Jianzhong Shi Guohua Deng Jing Guo Xianying Zeng Xijun He Huihui Kong Chunyang Gu Xuyong Li Jinxiong Liu Guojun Wang Yan Chen Liling Liu Libin Liang Yuanyuan Li Jun Fan Jinliang Wang Wenhui Li Lizheng Guan Qimeng Li Huanliang Yang Pucheng Che | 2013 | Science2013，，6144： | 1 |
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