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| 1 | Physical location of rice Gm-6,Pi-5(t) genes in O.officinalis with BAC-FISH显示文摘A fluorescence in situ hybridization (FISH) procedure was adopted to physically map two rice BAC clones 24E21 and 4F22 linked to Gm-6 and Pi-5(t) in O. offi-cinalis. FISH results showed that the two BAC clones were located at 4L. The percentage distance from the centromere to the hybridization sites was 72 ± 2.62 for 24E21 and 54± 5.43 for 4F22, the detection rates were 52.70% and 61.2%. The results obtained from the BAC and plasmid clones, RG214 and RZ565 of cultivated rice and O. officinalis were the same. This suggested that the markers, RG214 and RZ565 of cultivated rice and O. officinalis were in the same BAC clones. The homologous sequences of Gm-6 and Pi-5(t) in O. officinalis were positions that signals existed on the 4L. Many signals were observed when no Cot-1 DNA blocked. This also showed that repetitive sequences were some ho-molgous between cultivated rice and O. officinalis. The identification of chromosome 4 of O. officinalis is based on Jena et al. (1994). In our study, we discussed | QIN Rui WEI Wenhui JIN Weiwei HE Guangcun NING Shunbin YU Shunwu SONG Yunchun | 2001 | Chinese Science Bulletin2001，46，8： | 6 |
| 2 | Comparative genome research between maize and rice using genomic in situ hybridization显示文摘Using the genomic DNAs of maize and rice as probes respectively, the homology of maize and rice genomes was assessed by genomic in situ hybridization. When rice genomic DNAs were hybridized to maize, all chromosomes displayed many multiple discrete regions, while each rice chromosome delineated a single consecutive chromosomal region after they were hybridized with maize genomic DNAs. The results indicate that the genomes of maize and rice share high homology, and confirm the proposal that maize and rice are diverged from a common ancestor. | Shunbin Ning Weiwei Jin Ling Wang Yi Ding Yunchun Song | 2001 | Chinese Science Bulletin2001，46，8： | 5 |
| 3 | Determination of copy number for 5S rDNA and centromeric sequence RCS2 in rice by Fiber-FISH显示文摘The copy number of 5S rDNA and centromerie sequence RCS2 was determined by extended DNA fiber based fluorescence in situ hybridization (Fiber-FISH) in rice (Oryza sativa ssp. indica cv. Guangluai No. 4) genome. In order to determine the copy number, it is necessary to know the basepair number that a given length DNA fiber contains under a microscope. Therefore, the length of two DNA frag-ments, in which the basepair number had been already known, was measured. The insert sequence of the tested BAC 38D17 was 136 kb and its extended DNA was 56.4 μm long, 2.41 kb/μm on average, while that of the tested BAC 44B4 was 144.5 kb in total and 55.7 μm long, 2.60 kb/μm on average under the microscope. They were very close to the theoretical value of B-DNA in the Watson-Crick DNA model, which is 2.97 kb/μm. According to the average value of basepair number per μm of the two samples mentioned above, that is, 2.51 kb/μm, it could be estimated that the copy number was about 686 for 5S rDNA and 286-1121 for the | Zongyun Li Siluo Huang Weiwei Jin Shunbin Ning Yunchun Song Lijia Li | 2002 | Chinese Science Bulletin2002，47，3： | 5 |
| 4 | A novel method for in situ detection of apoptotic cell death in plants显示文摘A simple novel protocol suitable for in situ detection of apoptotic plant cell death is presented. This protocol combines the chromosome spreading technique with an extended in situ end labeling version for plants and makes it possible to detect apoptosis directly at chromosome,nuclear and DNA levels simultaneously with high-sensitivity. False positive and nonspecific signals can be efficiently avoided. Moreover, the changes of chromosomes, nuclei and DNA during the process of apoptosis can be characterized in situ. To illustrate this method, salt stress-induced cell death has been investigated in maize root meristematic cells. | Shunbin Ning Yunchun Song Ling Wang Lihua Liu | 1999 | Chinese Science Bulletin1999，44，11： | 4 |
| 5 | FISH analysis of the integration patterns in transgenic rice co-transformed by micro-projectile bombardment显示文摘Using multi-color fluorescence in situ hybridization (FISH), we localized transferred barnase-psl and pHctinG DNA sequences onto chromosomes of two trans-genie rice plants, named Q12 and Q13, both of which were produced by micro-projectile bombardment. In both Q12 and Q13, each detected cell showed 2-3 signal spots on their chromosomes respectively. The signals of both barnase-psl and pHctinG were mostly detected in the adjacent chromosomal sites in which their signals were overlapped and could be recognized by the signal color on the metaphase chromosomes. Fiber FISH further demonstrated that the multiple copies in each of the two DNA sequences distributed adjacently on the DNA fiber in Q13. Combined with the results of Southern hybridization, the possible integration patterns in transgenic rice co-transformed by micro-projectile bombardment have been discussed. | JIN Weiwei LI Zongyun NING Shunbin LING Dinghou LI Lijia SONG Yunchun | 2001 | Chinese Science Bulletin2001，46，23： | 1 |
| 6 | Test and analysis of the influence of the transformer insulation resistance and dielectric loss显示文摘 | ZHANG Mengjie HU Shunbin SUN JiaLong XU Hai ZHANG Ning | 2015 | International English Education Research2015，，10： | 0 |
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