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| 1 | Rockburst mechanism research and its control显示文摘A new rockburst classification, innovative works in developing a ‘‘strainburst test machine' and an‘‘impact-induced rockburst test machine' that can reproduce rockbursts in laboratory were researched.New concepts were proposed regarding the stress paths that take into account both the static and dynamic stresses analogous to that at excavation boundaries for generating artificially-induced strainburst and impact-induced rockburst. As an important method for rockburst control, a novel energyabsorbing bolt was developed, which has a constant-resistance under both static and impact loadings and a large-elongation capacity for containing large deformations of rock masses under burst-prone conditions. | Manchao He Fuqiang Ren Dongqiao Liu | 2018 | International Journal of Mining Science and Technology2018，28，5： | 21 |
| 2 | Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution显示文摘The speed of high-resolution optical imaging has been a rate-limiting factor for meso-scale mapping of brain structures and functional circuits,which is of fundamental importance for neuroscience research.Here,we describe a new microscopy method of Volumetric Imaging with Synchronized on-the-fly-scan and Readout(VISo R)for high-throughput,high-quality brain mapping.Combining synchronized scanning beam illumination and oblique imaging over cleared tissue sections in smooth motion,the VISo R system effectively eliminates motion blur to obtain undistorted images.By continuously imaging moving samples without stopping,the system achieves high-speed 3D image acquisition of an entire mouse brain within1.5 hours,at a resolution capable of visualizing synaptic spines.A pipeline is developed for sample preparation,imaging,3D image reconstruction and quantification.Our approach is compatible with immunofluorescence methods,enabling flexible cell-type specific brain mapping and is readily scalable for large biological samples such as primate brains.Using this system,we examined behaviorally relevant whole-brain neuronal activation in 16 c-Fos-sh EGFP mice under resting or forced swimming conditions.Our results indicate the involvement of multiple subcortical areas in stress response.Intriguingly,neuronal activation in these areas exhibits striking individual variability among different animals,suggesting the necessity of sufficient cohort size for such studies. | Hao Wang Qingyuan Zhu Lufeng Ding Yan Shen Chao-Yu Yang Fang Xu Chang Shu Yujie Guo Zhiwei Xiong Qinghong Shan Fan Jia Peng Su Qian-Ru Yang Bing Li Yuxiao Cheng Xiaobin He Xi Chen Feng Wu Jiang-Ning Zhou Fuqiang Xu Hua Han Pak-Ming Lau Guo-Qiang Bi | 2019 | National Science Review2019，6，5： | 7 |
| 3 | Detection and characterization of diverse alpha-and betacoronaviruses from bats in China显示文摘Bats have been implicated as important reservoir hosts of alpha- and betacoronaviruses. In this study, diverse coronaviruses(CoVs) were detected in 50 of 951(positive rate 5.3%) intestinal specimens of eight bat species collected in four provinces and the Tibet Autonomous Region of China by pan-coronavirus RT-PCR screening. Based on 400-nt RNA-dependent RNA polymerase(Rd RP) sequence analysis, eight belonged to genus Alphacoronavirus and 42 to Betacoronavirus.Among the 50 positive specimens, thirteen gave rise to CoV full-length Rd RP gene amplification for further sequence comparison, of which three divergent sequences(two from a unreported province) were subjected to full genome sequencing. Two complete genomes of betacoronaviruses(JTMC15 and JPDB144) and one nearly-complete genome of alphacoronavirus(JTAC2) were sequenced and their genomic organization predicted. The present study has identified additional numbers of genetically diverse bat-borne coronaviruses with a wide distribution in China. Two new species of bat CoV, identified through sequence comparison and phylogenetic analysis, are proposed. | Lin Xu Fuqiang Zhang Weihong Yang Tinglei Jiang Guanjun Lu Biao He Xingyu Li Tingsong Hu Gang Chen Yun Feng Yuzhen Zhang Quanshui Fan Jiang Feng Hailin Zhang Changchun Tu | 2016 | Virologica Sinica2016，31，1： | 6 |
| 4 | Cortical Organization of Centrifugal Afferents to the Olfactory Bulb: Mono-and Trans-synaptic Tracing with Recombinant Neurotropic Viral Tracers显示文摘Sensory processing is strongly modulated by different brain and behavioral states,and this is based on the top-down modulation.In the olfactory system,local neural circuits in the olfactory bulb(OB)are innervated by centrifugal afferents in order to regulate the processing of olfactory information in the OB under different behavioral states.The purpose of the present study was to explore the organization of neural networks in olfactory-related cortices and modulatory nuclei that give rise to direct and indirect innervations to the glomerular layer(GL)of the OB at the whole-brain scale.Injection of different recombinant attenuated neurotropic viruses into the GL showed that it received direct inputs from each layer in the OB,centrifugal inputs from the ipsilateralanterior olfactory nucleus(AON),anterior piriform cortex(Pir),and horizontal limb of diagonal band of Broca(HDB),and various indirect inputs from bilateral cortical neurons in the AON,Pir,amygdala,entorhinal cortex,hippocampus,HDB,dorsal raphe,median raphe and locus coeruleus.These results provide a circuitry basis that will help further understand the mechanism by which olfactory informationprocessing in the OB is regulated. | Pengjie Wen Xiaoping Rao Liuying Xu Zhijian Zhang Fan Jia Xiaobin He Fuqiang Xu | 2019 | Neuroscience Bulletin2019，35，4： | 4 |
| 5 | Regional Metabolic Patterns of Abnormal Postoperative Behavioral Performance in Aged Mice Assessed by XH-NMR Dynamic Mapping Method显示文摘Abnormal postoperative neurobehavioral performance(APNP)is a common phenomenon in the early postoperative period.The disturbed homeostatic status of metabolites in the brain after anesthesia and surgery might make a significant contribution to APNP.The dynamic changes of metabolites in different brain regions after anesthesia and surgery,as well as their potential association with APNP are still not well understood.Here,we used a battery of behavioral tests to assess the effects of laparotomy under isoflurane anesthesia in aged mice,and investigated the metabolites in 12 different sub-regions of the brain at different time points using proton nuclear magnetic resonance('H-NMR)spectroscopy.The abnormal neurobehavioral performance occurred at 6 h and/or 9 h,and recovered at 24 h after anesthesia/surgery.Compared with the control group,the altered metabolite of the model group at 6 h was aspartate(Asp),and the difference was mainly displayed in the cortex;while significant changes at 9 h occurred predominantly in the cortex and hippocampus,and the corresponding metabolites were Asp and glutamate(Glu).All changes returned to baseline at 24 h.The altered metabolic changes could have occurred as a result of the acute APNP,and the metabolites Asp and Glu in the cortex and hippocampus could provide preliminary evidence for understanding the APNP process. | Taotao Liu Zhengqian Li Jindan He Ning Yang Dengyang Han Yue Li Xuebi Tian Huili Liu Anne Manyande Hongbing Xiang Fuqiang Xu Jie Wang Xiangyang Guo | 2020 | Neuroscience Bulletin2020，36，1： | 2 |
| 6 | Regulation of pathological BBB restoration via nanostructured ROS-responsive glycolipid-like copolymer entrapping siVEGF for glioblastoma targeted therapeutics显示文摘Glioblastoma(GBM)is one of the malignant brain tumors with high mortality and no curative treatments.Abnormally elevated vascular endothelial growth factor(VEGF)in GBM seriously disrupts the blood brain barrier(BBB)with an increased permeability,resulting in poor outcome and prognosis.RNAi interference has shown strong potential to inhibit VEGF expression,thus it is necessary to development an effective and safe gene delivery system possessing the ability to cross the BBB and target GBM cells.This study aims to explore the anti-GBM effect of angiopep-2(Ap)peptide modified reactive oxygen species(ROS)cleavable thioketal(TK)linked glycolipid-like nanocarrier(CSTKSA)delivering anti-VEGF siRNA(R),termed as Ap-CSTKSA/R complexes.Ap functionalized modification produced an enhanced cellular uptake and a stronger bio-distribution of Ap-CSTKSA/R complexes in U87 MG cells and brain tumor tissues,respectively.Ap-CSTKSA/R complexes exhibited great superiority in GBM growth inhibition and finally translated into the longest survival period mainly via receptor-mediated targeting delivery,VEGF gene silencing accompanied with remarkable angiogenesis inhibition,and suppressed expression of caveolin-1 which is involved in BBB functional regulation in the occurrence and treatment of GBM.The study indicated that Ap functionalization on ROS-responsive glycolipid-like copolymer exhibits a promising and effective gene delivery platform for GBM targeted treatment. | Lijuan Wen Yu Peng Kai Wang Zhihua Huang Shiying He Ruiwen Xiong Longping Wu Fengtian Zhang Fuqiang Hu | 2022 | Nano Research2022，15，2： | 2 |
| 7 | Rabies Virus Pseudotyped with CVS-N2C Glycoprotein as a Powerful Tool for Retrograde Neuronal Network Tracing显示文摘Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain. The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2 C glycoprotein(SAD-RV(DG)-N2 C(G)) shows strong neuro-tropism in cell culture, but its in vivo efficiency for retrograde gene transduction and neuro-tropism have not been systematically characterized.We compared these features in different mouse brain regions for SAD-RV-N2 C(G) and two other widely-used retrograde tracers, SAD-RV(DG)-B19(G) and r AAV2-retro. We found that SAD-RV(DG)-N2 C(G) enhanced the infection efficiency of long-projecting neurons by^10 times but with very similar neuro-tropism, compared with SAD-RV(DG)-B19(G). On the other hand, SAD-RV(DG)-N2 C(G) had an infection efficiency comparable with r AAV2-retro, but a more restricted diffusion range, and broader tropism to different types and regions of longprojecting neuronal populations. These results demonstrate that SAD-RV(DG)-N2 C(G) can serve as an effective retrograde vector for studying neuronal circuits. | Xutao Zhu Kunzhang Lin Qing Liu Xinpei Yue Huijie Mi Xiaoping Huang Xiaobin He Ruiqi Wu Danhao Zheng Dong Wei Liangliang Jia Weilin Wang Anne Manyande Jie Wang Zhijian Zhang Fuqiang Xu | 2020 | Neuroscience Bulletin2020，36，3： | 2 |
| 8 | Plasmonic coupling-enhanced in situ photothermal nanoreactor with shape selective catalysis for C–C coupling reaction显示文摘Carbon-carbon(C–C)coupling reactions represent one of the most powerful tools for the synthesis of complex natural products,bioactive molecules developed as drugs and agrochemicals.In this work,a multifunctional nanoreactor for C–C coupling reaction was successfully fabricated via encapsulating the core-shell Cu@Ni nanocubes into ZIF-8(Cu@Ni@ZIF-8).In this nanoreactor,Ni shell of the core-shell Cu@Ni nanocubes was the catalytical active center,and Cu core was in situ heating source for the catalyst by absorbing the visible light.Moreover,benefiting from the plasmonic resonance effect between Cu@Ni nanocubes encapsulated in ZIF-8,the absorption range of nanoreactor was widened and the utilization rate of visible light was enhanced.Most importantly,the microporous structure of ZIF-8 provided shape-selective of reactant.This composite was used for the highly shape-selective and stable photocatalysed C–C coupling reaction of boric acid under visible light irradiation.After five cycles,the nanoreactor still remained high catalytical activity.This Cu@Ni@ZIF-8 nanoreactor opens a way for photocatalytic C–C coupling reactions with shape-selectivity. | Zhenxing Li Yixuan Gong Xin Zhang Yangyang Wen Jiasai Yao Mingliang Hu Miao He Jiahao Liu Rui Li Fuqiang Wang Chuanxin Zhang | 2020 | Nano Research2020，13，10： | 2 |
| 9 | Overexpression of Sirt6 ameliorates sleep deprivation induced-cognitive impairment by modulating glutamatergic neuron function显示文摘Sleep benefits the restoration of energy metabolism and thereby suppo rts neuronal plasticity and cognitive behaviors.Sirt6 is a NAD+-dependent protein deacetylase that has been recognized as an essential regulator of energy metabolism because it modulates various transcriptional regulators and metabolic enzymes.The aim of this study was to investigate the influence of Sirt6 on cerebral function after chronic sleep deprivation(CSD).We assigned C57BL/6J mice to control or two CSD groups and subjected them to AAV2/9-CMV-EGFP or AAV2/9-CMV-Sirt6-EGFP infection in the prelimbic cortex(PrL).We then assessed cerebral functional connectivity(FC) using resting-state functional MRI,neuron/astrocyte metabolism using a metabolic kinetics analysis;dendritic spine densities using sparse-labeling;and miniature excitato ry postsynaptic currents(mEPSCs) and action potential(AP) firing rates using whole-cell patchclamp recordings.In addition,we evaluated cognition via a comprehensive set of behavioral tests.Compared with controls,Sirt6 was significantly decreased(P<0.05) in the PrL after CSD,accompanied by cognitive deficits and decreased FC between the PrL and accumbens nucleus,piriform cortex,motor co rtex,somatosensory co rtex,olfactory tubercle,insular cortex,and cerebellum.Sirt6 ove rexpression reve rsed CSD-induced cognitive impairment and reduced FC.Our analysis of metabolic kinetics using [1-13C] glucose and [2-13C] acetate showed that CSD reduced neuronal Glu4and GABA2synthesis,which could be fully restored via forced Sirt6 expression.Furthermore,Sirt6 ove rexpression reversed CSD-induced decreases in AP firing rates as well as the frequency and amplitude of mEPSCs in PrL pyramidal neurons.These data indicate that Sirt6 can improve cognitive impairment after CSD by regulating the PrL-associated FC network,neuronal glucose metabolism,and glutamatergic neurotransmission.Thus,Sirt6 activation may have potential as a novel strategy for treating sleep disorder-related diseases. | Jinpiao Zhu Chang Chen Zhen Li Xiaodong Liu Jingang He Ziyue Zhao Mengying He Binbin Nie Zili Liu Yingying Chen Kuanpin Su Xiang Li Juxiang Chen Hongbing Xiang Fuqiang Xu Kangguang Lin Zongze Zhang Jie Wang | 2023 | Neural Regeneration Research2023，18，11： | 1 |
| 10 | The influence of Amorpha fruticosa on the growth of Populus ussuriensis under the inoculation of AM fungi 显示文摘 | Fuqiang Song Xingjun Tian Xingbing He | 2009 | New forests2009，37，： | 1 |
| 11 | Single-Cell Exome Sequencing Reveals Single-Nucleotide Mutation Characteristics of a Kidney Tumor显示文摘 | Xun Xu Yong Hou Xuyang Yin Li Bao Aifa Tang Luting Song Fuqiang Li Shirley Tsang Kui Wu Hanjie Wu Weiming He Liang Zeng Manjie Xing Renhua Wu Hui Jiang Xiao Liu Dandan Cao Guangwu Guo Xueda Hu Yaoting Gui Zesong Li Wenyue Xie Xiaojuan Sun Min Shi Zhiming | 2012 | Cell2012，，5： | 1 |
| 12 | Non-host plants:Are they mycorrhizal networks players?显示文摘Common mycorrhizal networks(CMNs)that connect individual plants of the same or different species together play important roles in nutrient and signal transportation,and plant community organization.However,about 10%of land plants are non-mycorrhizal species with roots that do not form any wellrecognized types of mycorrhizas;and each mycorrhizal fungus can only colonize a limited number of plant species,resulting in numerous non-host plants that could not establish typical mycorrhizal symbiosis with a specific mycorrhizal fungus.If and how non-mycorrhizal or non-host plants are able to involve in CMNs remains unclear.Here we summarize studies focusing on mycorrhizal-mediated host and non-host plant interaction.Evidence has showed that some host-supported both arbuscular mycorrhizal(AM)and ectomycorrhizal(EM)hyphae can access to non-host plant roots without forming typical mycorrhizal structures,while such non-typical mycorrhizal colonization often inhibits the growth but enhances the induced system resistance of non-host plants.Meanwhile,the host growth is also differentially affected,depending on plant and fungi species.Molecular analyses suggested that the AMF colonization to non-hosts is different from pathogenic and endophytic fungi colonization,and the hyphae in non-host roots may be alive and have some unknown functions.Thus we propose that non-host plants are also important CMNs players.Using non-mycorrhizal model species Arabidopsis,tripartite culture system and new technologies such as nanoscale secondary ion mass spectrometry and multiomics,to study nutrient and signal transportation between host and non-host plants via CMNs may provide new insights into the mechanisms underlying benefits of intercropping and agro-forestry systems,as well as plant community establishment and stability. | Yanliang Wang Xinhua He Fuqiang Yu | 2022 | Plant Diversity2022，44，2： | 1 |
| 13 | Automatic visual inspection for leather manufacture 显示文摘 | HE Fuqiang WANG Wen CHEN Zichen | 2006 | Key Eng Mater2006，，12： | 1 |
| 14 | AgNO3-based colorimetric methods for measurement of chloride penetration in concrete显示文摘 | HE Fuqiang SHI Caijun | | 0，，01： | 1 |
| 15 | Bridging small-gap peripheral nerve defects using biodegradable chintin conduit with cultured cells in rats 显示文摘 | Peixun Zhang Xiangjun He Fuqiang Zhao | 2005 | Journal of reconstructive microsurgery2005，8，21： | 1 |
| 16 | Imaging and Spectral Characteristics of Amyloid Plaque Autofluorescence in Brain Slices from the APP/PS1 Mouse Model of Alzheimer’s Disease显示文摘Amyloid deposits are one of the hallmark pathological lesions of Alzheimer's disease(AD). They can be visualized by thioflavin-S, silver impregnation,Congo red staining, and immunohistochemical reactions.However, that amyloid deposits generate blue autofluorescence(auto-F) has been ignored. Here, we report that visible light-induced auto-F of senile plaques(SPs) was detected and validated with conventional methods. Brain slices from APP/PS1(amyloid precursor protein/presenilin1) transgenic mice were mounted on slides, rinsed,coverslipped and observed for details of the imaging and spectral characteristics of the auto-F of SPs. Then the slices were treated with the above classic methods for comparative validation. We found that the SP auto-F was greatest under blue-violet excitation with a specific emission spectrum, and was much easier, more sensitive, and reliable than the classic methods. Because it does not damage slices, observation of auto-F can be combined with all post-staining techniques in slices and for brain-wide imaging in AD. | Yunling Gao Qing Liu Lingling Xu Ning Zheng Xiaoming He Fuqiang Xu | 2019 | Neuroscience Bulletin2019，35，6： | 1 |
| 17 | 一个中央杏仁核—无名质神经环路编码厌恶增强信号(英文)显示文摘文章简介Aversive stimuli can impact motivation and support associative learning as reinforcers.However,the neural circuitry underlying the processing of aversive reinforcers has not been elucidated.Here,we report | Yuting Cui Guanghui Lv Sen Jin Jie Peng Jing Yuan Xiaobin He Hui Gong Fuqiang Xu 许彤辉 李浩洪 | 2018 | 科学新闻2018，0，4： | 0 |
| 18 | Synergy of first-and second-sphere interactions in a covalent organic framework boosts highly selective platinum uptake显示文摘Platinum recovery from waste electrical and electronic equipment(WEEE) in highly acidic solutions is significant to the electronics industry and environmental remediation. However, the lack of ingenious design and synergetic coordination gives rise to unsatisfied PtCl_(4)^(2-)extraction capacities and selectivities in most previously reported adsorbents(e.g., polymeric and inorganic materials). Herein, we proposed a synergistic strategy that realizes highly selective PtCl_(4)^(2-)uptake through first-and second-sphere coordinations. The proof-of-concept imine-linked covalent organic framework(SCU-COF-3) was found to chelate Pt Cl42-via the direct N…Pt coordination and the synergistically interlaminar N–H…Cl hydrogen bonds, which was disclosed by the comprehensive analysis of extended X-ray adsorption fine structure(EXAFS) characterizations and density functional theory(DFT) calculations. The unique adsorption mechanism imparts a superior adsorption capacity(168.4 mg g-1)and extraordinary Pt(II) selectivity to SCU-COF-3 under static conditions. In addition, SCU-COF-3 exhibits an upgraded distribution coefficient of 1.62 × 10^(5)mL· g^(-1), one order of magnitude higher than those of reported natural adsorbents. Specifically, SCU-COF-3 can extract PtCl_(4)^(2- )quantitatively from a simulated acidic waste solution coexisting with other 12 competitive ions, suggesting its promising application in practical scenarios. | Linwei He Baoyu Li Zhonglin Ma Lixi Chen Shicheng Gong Mingxing Zhang Yaoyao Bai Qi Guo Fuqi Wu Fuqiang Zhao Jie Li Duo Zhang Daopeng Sheng Xing Dai Long Chen Jie Shu Zhifang Chai Shuao Wang | 2023 | Science China Chemistry2023，66，3： | 0 |
| 19 | A method for detecting two-dimensional plane stress distribution in basin-type insulator based on critically refracted longitudinal wave显示文摘Basin-type insulator often has small cracks due to stress concentration.The current method cannot accurately reflect the stress condition of the insulator to find the stress concentration areas.To solve these problems,a method for detecting two-dimensional plane stress(δ_(1) andδ_(2))within different depth ranges in a basin-type insulator is proposed based on critically refracted longitudinal(LCR)wave.First,the acoustoelastic equation characterising the relationship between the variation of LCR wave propagation time and the plane stress was derived.Next,the propagation characteristics of LCR wave in epoxy resin samples were investigated.Then,the stress distribution within different depth ranges of the insulator subjected to hydraulic load was measured using the proposed method,including direction(θ),δ_(1) andδ_(2).The results show that the magnitude of the stress alone cannot accurately characterise the stress state.Points with equal distances to the centre have similar stress magnitudes,but their directions are not the same.With increasing depth,θremains essentially unchanged at the same location,whileδ1 andδ2 decrease,and the rate of decrease varies at different locations.Comparing the measured and simulated data,the results showed that they were in good agreement,and the maximum errors of stress value andθwere 0.69 MPa and 2.97°,respectively,which confirmed the feasibility and accuracy of the stress detection in the proposed method. | Zhaoyang Kang Fuqiang Ren Hongru Zhang Jingjing Yang Kaining Hou Qingquan Li Dongxin He Hongshun Liu Yongzhi Zhao Huaxin Wen | 2024 | High Voltage2024，9，1： | 0 |
| 20 | SYNTHESIS OF NEW POLY (p-PHENYLENEVINYLENE) DERIVATIVES WITH IMPROVED SOLUBILITY显示文摘PPV-based copolymers with cyano group in the main chain have been synthesized by Wittig-type polycondensation of alkoxy-substituted dialdehydes and 4,4’-bis(cyanomethyl)-substituted diphenyl ether: [―C6H4―O―C6H4―C(CN)(CH―C6H2(OR)2―C(C(CN)―]n (POPPV). The copolymers are highly soluble in common solvents and their super highly transparent homogeneous thin films can be formed by spin coating directly without heat treatment. The chemical structures are determined by NMR and IR spectroscopys and evaluated using photoluminescence, gel-permeation chromatography (GPC) and DSC (or TGA) methods. The incorporation of ether group into the PPV backbone has an interruption of the main-chain conjugation, that results in the blue-shift of electroluminescence spectra and enhancement the solubility. | ZHANG Fuqiang YIN Shougen LIU Xiaohang HUANG Wenqiang HE Binling | 2000 | Chinese Journal of Reactive Polymers2000，9，2： | 0 |
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