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| 1 | Diagnosis of intestinal tuberculosis using a monoclonal antibody to Mycobacterium tuberculosis显示文摘AIM:To investigate the utility of immunohistochemical(IHC) staining with an antibody to Mycobacterium tuberculosis(M.tuberculosis) for the diagnosis of intestinal tuberculosis(TB).METHODS:We retrospectively identified 10 patients(4 males and 6 females;mean age = 65.1 ± 13.6 years) with intestinal TB.Clinical characteristics,including age,gender,underlying disease,and symptoms were obtained.Chest radiograph and laboratory tests,including sputum Ziehl-Neelsen(ZN) staining,M.tuberculosis culture,and sputum polymerase chain reaction(PCR) for tubercle bacilli DNA,as well as Tuberculin skin test(TST) and QuantiFERON-TB gold test(QFT),were examined.Colonoscopic records recorded on the basis of Sato's classification were also reviewed,in addition to data from intestinal biopsies examined for histopathological findings,including hematoxylin and eosin staining,and ZN staining,as well as M.tuberculosis culture,and PCR for tubercle bacilli DNA.For the present study,archived formalin-fixed paraffin-embedded(FFPE) intestinal tissue samples were immunohistochemically stained using a commercially available species-specific monoclonal antibody to the 38-kDa antigen of the M.tuberculosis complex.These sections were also stained with the pan-macrophage marker CD68 antibody.RESULTS:From the clinical data,we found that no patients were immunocompromised,and that the main symptoms were diarrhea and weight loss.Three patients displayed active pulmonary TB,six patients(60%) had a positive TST,and 4 patients(40%) had a positive QFT.Colonoscopic findings revealed that all patients had type 1 findings(linear ulcers in a circumferential arrangement or linear ulcers arranged circumferentially with mucosa showing multiple nodules),all of which were located in the right hemicolon and/or terminal ileum.Seven patients(70%) had concomitant healed lesions in the ileocecal area.No acid-fast bacilli were detected with ZN staining of the intestinal tissue samples,and both M.tuberculosis culture and PCR for tubercle bacilli DNA were negative in all samples.The histopathological data revealed that tuberculous granulomas were present in 4 cases(40%).IHC staining in archived FFPE samples with anti-M.tuberculosis monoclonal antibody revealed positive findings in 4 patients(40%);the same patients in which granulomas were detected by hematoxylin and eosin staining.M.tuberculosis antigens were found to be mostly intracellular,granular in pattern,and primarily located in the CD68 + macrophages of the granulomas.CONCLUSION:IHC staining with a monoclonal antibody to M.tuberculosis may be an efficient and simple diagnostic tool in addition to classic examination methods for the diagnosis of intestinal TB. | Yasushi Ihama Akira Hokama Kenji Hibiya Kazuto Kishimoto Manabu Nakamoto Tetsuo Hirata Nagisa Kinjo Haley L Cash Futoshi Higa Masao Tateyama Fukunori Kinjo Jiro Fujita | 2012 | World Journal of Gastroenterology2012，18，47： | 12 |
| 2 | Prostate tumor neuroendocrine differentiation via EMT: The road less traveled显示文摘The long-standing challenge in the treatment of prostate cancer is to overcome therapeutic resistance during progression to lethal disease.Aberrant transforming-growth factor-b(TGF-b)signaling accelerates prostate tumor progression in a transgenic mouse model via effects on epithelial-mesenchymal transition(EMT),and neuroendocrine differentiation driving tumor progression to castration-resistant prostate cancer(CRPC).Neuroendocrine prostate cancer(NEPC)is highly aggressive exhibiting reactivation of developmental programs associated with EMT induction and stem cell-like characteristics.The androgen receptor(AR)is a critical driver of tumor progression as well as therapeutic response in patients with metastatic CRPC.The signaling interactions between the TGF-β mechanistic network and AR axis impact the EMT phenotypic conversions,and perturbation of epithelial homeostasis via EMT renders a critical venue for epithelial derived tumors to become invasive,acquire the neuroendocrine phenotype,and rapidly metastasize.Combinations of microtubule targeting taxane chemotherapy and androgen/AR targeting therapies have survival benefits in CRPC patients,but therapeutic resistance invariability develops,leading to mortality.Compelling evidence from our group recently demonstrated that chemotherapy(cabazitaxel,second line taxane chemotherapy),or TGF-β receptor signaling targeted therapy,caused reversion of EMT to mesenchymal-epithelial transition and tumor re-differentiation,in in vitro and in vivo prostate cancer models.In this review,we discuss the functional contribution of EMT dynamic changes to the development of the neuroendocrine phenotypedthe newly characterized pathological feature of prostate tumors in the context of the tumor microenvironment-navigated cell lineage changes and the role of this neuroendocrine phenotype in metastatic progression and therapeutic resistance. | Haley Dicken Patrick J.Hensley Natasha Kyprianou | 2019 | Asian Journal of Urology2019，6，1： | 3 |
| 3 | Evidence and explanation for the involvement of the nucleus accumbens in pain processing显示文摘The nucleus accumbens(NAc)is a subcortical brain structure known primarily for its roles in pleasure,reward,and addiction.Despite less focus on the NAc in pain research,it also plays a large role in the mediation of pain and is effective as a source of analgesia.Evidence for this involvement lies in the NAc’s cortical connections,functions,pharmacology,and therapeutic targeting.The NAc projects to and receives information from notable pain structures,such as the prefrontal cortex,anterior cingulate cortex,periaqueductal gray,habenula,thalamus,etc.Additionally,the NAc and other pain-modulating structures share functions involving opioid regulation and motivational and emotional processing,which each work beyond simply the rewarding experience of pain offset.Pharmacologically speaking,the NAc responds heavily to painful stimuli,due to its high density ofμopioid receptors and the activation of several different neurotransmitter systems in the NAc,such as opioids,dopamine,calcitonin gene-related peptide,γ-aminobutyric acid,glutamate,and substance P,each of which have been shown to elicit analgesic effects.In both preclinical and clinical models,deep brain stimulation of the NAc has elicited successful analgesia.The multi-functional NAc is important in motivational behavior,and the motivation for avoiding pain is just as important to survival as the motivation for seeking pleasure.It is possible,then,that the NAc must be involved in both pleasure and pain in order to help determine the motivational salience of positive and negative events. | Haley N.Harris Yuan B.Peng | 2020 | Neural Regeneration Research2020，15，4： | 3 |
| 4 | 对两种不同饲养条件下猪生长和胴体性状的遗传与表型参数的研究显示文摘本文对自由采食和限制喂料两群猪的平均日增重(DG)、平均日采食量(FI)、饲料转化率(FCR)、P_2点背膘厚(BF)和后腿瘦肉量(HL)作了遗传分析和比较。试验猪为合成品系,主要的遗传血统为大白猪和长白猪以及少量的巴克夏猪。限制采食猪各性状的平均数和标准差为:DG 729±48.45g,FI 1.85±0.043kg,FCR 2.56±0.15,BF 15.3±1.90mlmHL 6.07±0.36kg,自由采食猪相应为:821±85.87g,2.31±0.16 kg,2.85±0.31,16.8±2.43mm,6.07±0.56kg。限制采食猪各性状的遗传力:DG 0.399,FI 0.410,FC R0.403,BF 0.619,HL 0.446,而自由采食猪分别为0.307,0.480,0.129,0.315和0.350。在限制采食下,日增重与饲料转化率、日增重与后腿瘦肉量、饲料转化率与后腿瘦肉量有很强的遗传相关,分别为-0.94,0.95和-0.97;而在自由采食下这三个相关分别为-0.46,0.97和-0.46。 | 胡锦平 Chris Haley Cam Mcphee | 1990 | 畜牧兽医学报1990，21，2： | 3 |
| 5 | Cell:癌基因“遗传平衡”或会影响患者对癌症疗法的反应显示文摘进行癌症疗法的选择越来越需要确定肿瘤细胞中是否含有一些特殊突变的癌基因会驱动癌细胞异常生长。并且确定是否癌细胞会对特殊药物耐受或敏感.近日来自加州大学旧金山分校的研究人员就通过研究发现。以常见的癌基因KRAs突变为例。患者对疗法的反应往往会随着肿瘤的不断进化而发生改变。相关研究刊登于国际杂志Cell上。 | Michael R. Burgess Eugene Hwang Rana Mroue Craig M. Bielski Anica M. Wandler Benjamin J. Huang Ari J. Firestone Amy Young Jennifer A. Lacap Lisa Crocker Saurabh Asthana Elizabeth M. Davis Jin Xu Keiko Akagi Michelle M. Le Beau Qing Li Benjamin Haley David Stokoe Deepak Sampath Barry S. Taylor Marie Evangelista Kevin Shannon | 2017 | 现代生物医学进展2017，17，18： | 2 |
| 6 | 太湖猪种质基因特征研究显示文摘采用PCRRFLPs、PCRSSCPs和PCRDSCPs分析方法,对我国太湖猪(二花脸猪和梅山猪)18个功能基因特征进行了研究,并与大白猪进行了比较。18个功能基因是:乌头酸酶2(ACO2)、缩胆囊素肠促胰酶肽(CCK)、17β雌二醇脱氢酶(ED)、雌激素受体(ESR)、血纤维蛋白原(FGG)、生长激素(GH)、黏合素(HXB)、促黄体素释放激素受体(LHRHR)、白血病抑制因子(LIF)、莫洛尼鼠肉瘤病毒致肿瘤基因同系物(MOS)、黏病毒抗性1(MX1)、甲状旁腺激素(PTH)、磷酸葡萄糖酸脱氢酶(PGD)、维生素A结合蛋白2(RBP2)、T细胞受体α(TCRA)、T细胞受体β簇(TCRB)、转化生长因子β3(TGFB3)和甲状腺素运载蛋白(TTR)。研究结果表明,CCK、FGG、LHRHR、LIF和RBP2基因位点所扩增片段在两品种群内均呈单态性,而其他位点则呈多态性变异。太湖猪在ACO2、ED、ESR和TTR基因所扩增区域均已纯合固定,表现出保守性的一面;而在HXB、PGD和PTH位点上则出现多个等位基因,表现出变异性的一面。除在TGFB3基因位点外,太湖猪与大白猪在其他所有基因位点等位基因频率均有显著差异(P<0.01)。中国太湖猪的保守性与变异性在猪的基因定位、QTL定位及标记辅助选择中将起重要的作用。 | 姜志华 朱婉茹 徐世永 陈杰 黄瑞华 刘红林 Archibald A.L. Haley C.S. | 2005 | 南京农业大学学报2005，28，2： | 2 |
| 7 | Poly(ethylene glycol)hydrogel elasticity influences human mesenchymal stem cell behavior显示文摘Coordinated investigations into the interactions between biologically mimicking(biomimetic)material constructs and stem cells advance the potential for the regeneration and possible direct replacement of diseased cells and tissues.Any clinically relevant therapies will require the development and optimization of methods that mass produce fully functional cells and tissues.Despite advances in the design and synthesis of biomaterial scaffolds,one of the biggest obstacles facing tissue engineering is understanding how specific extracellular cues produced by biomaterial scaffolds influence the proliferation and differentiation of various cell sources.Matrix elasticity is one such tailorable property of synthetic scaffolds that is known to differ between tissues.Here,we investigate the interactions between an elastically tailorable polyethylene glycol(PEG)-based hydrogel platform and human bone marrow-derived mesenchymal stem cells(hMSCs).For these studies,two different hydrogel compositions with elastic moduli in the ranges of 50-60 kPa and 8-10 kPa were implemented.Our findings demonstrate that the different elasticities in this platform can produce changes in hMSC morphology and proliferation,indicating that the platform can be implemented to produce changes in hMSC behavior and cell state for a broad range of tissue engineering and regenerative applications.Furthermore,we show that the platform’s different elasticities influence stem cell differentiation potential,particularly when promoting stem cell differentiation toward cell types from tissues with stiffer elasticity.These findings add to the evolving and expanding library of information on stem cell-biomaterial interactions and opens the door for continued exploration into PEG-based hydrogel scaffolds for tissue engineering and regenerative medicine applications. | Anna K.Whitehead Haley H.Barnett Mary E.Caldorera-Moore Jamie J.Newman | 2018 | Regenerative Biomaterials2018，5，3： | 2 |
| 8 | Antenna design for a massive multiple input environmental sensor network显示文摘 | J. Craig Prather Michael Bolt Haley Harrell John Manobianco Mark L. Adams | 2016 | Digital Communications and Networks2016，2，4： | 2 |
| 9 | Integrative Genomic Profiling of Human Prostate Cancer显示文摘 | Barry S. Taylor Nikolaus Schultz Haley Hieronymus Anuradha Gopalan Yonghong Xiao Brett S. Carver Vivek K. Arora Poorvi Kaushik Ethan Cerami Boris Reva Yevgeniy Antipin Nicholas Mitsiades Thomas Landers Igor Dolgalev John E. Major Manda Wilson Nicholas D. | 2010 | Cancer Cell2010，，： | 2 |
| 10 | The nationwide nosocomial infection rate: a new need for vital statistics显示文摘 | Haley R Culver D White J | 1985 | Am J Epidemiol1985，121，： | 2 |
| 11 | 饲用酶和酵母衍生物可减轻球虫病对肉鸡肠道健康和功能的危害显示文摘球虫病引起的肠道坏死性病变会损害鸡的消化能力和肠道屏障功能,同时会增加继发性细菌感染的风险。养鸡业通过抗球虫药和免疫接种已成功控制球虫病。然而,人们对艾美耳球虫属的球虫对抗球虫药的耐药性、免疫空白期和限制使用抗生素的担忧,正在促进科研人员研究和应用替代性和/或补充策略来控制球虫病。本文的目的是评价有关介绍饲用酶和酵母衍生物在调控球虫病功效的文献资料。在受球虫病影响的鸡中,内源性酶(蛋白酶、淀粉酶和脂肪酶)的分泌量可能会变得不足,饲用酶可以弥补这些酶的缺乏。发生在小肠上端的球虫病为提高植酸酶的功效创造了条件,有报道表明补充植酸酶可以减轻球虫病对鸡骨骼质量的负面影响。补充纤维降解酶会增加肠道中短链脂肪酸的浓度,进而降低艾美耳球虫的存活率。有证据表明整个酵母(活的或死的)及其衍生物可以调控球虫病。研究证明,酵母衍生物的免疫调节特性可增强艾美耳球虫挑战模型中的细胞免疫和体液免疫,这对球虫苗的接种效果至关重要。此外,研究证明酵母核苷酸有利于促进动物肠道黏膜表面的愈合。其他新的研究表明,某些酵母细胞可以产生含有抗球虫化合物的衍生物,从而有效减少卵囊脱落。还有研究证明,酵母细胞表面是艾美耳球虫口服疫苗的一种高效载体。总体而言,虽然需要进一步完善研究以解决反应和商业性应用中的不一致问题,但有证据表明,饲用酶和酵母衍生物可以作为维持肉鸡肠道功能的补充策略,以提高患球虫病的肉鸡的生长性能。然而,肉鸡日粮含有多种不同作用模式的饲料添加剂,而且缺乏关于预期反应的实证数据。未来的评估应考虑添加剂的组合,以记录动物反应和潜在的协同作用。 | 王晶晶(译) 孟祥光(审) Elijah G.Kiarie Haley Leung Reza Akbari Moghaddam Kakhki Rob Patterson John R.Barta | 2022 | 国外畜牧学(猪与禽)2022，42，3： | 2 |
| 12 | General and specific combining abilities of larval and juvenile growth and viability estimated from natural oyster populations显示文摘 | A. L. Mallet L. E. Haley | 1984 | Marine Biology1984，，1： | 2 |
| 13 | Absence of porcine circovirus type 1 (PCV1) and high prevalence of PCV 2 exposure and infection in swine finisher herds显示文摘 | Sumathy Puvanendiran Suzanne Stone Wanqin Yu Craig R. Johnson Juan Abrahante Liza Garcia Jimenez Theodor Griggs Charles Haley Bruce Wagner Michael P. Murtaugh | 2011 | Virus Research2011，，1： | 2 |
| 14 | Super Decoup led Load Flow with Distribution Slack Bus 显示文摘 | Haley P H Ayres M | 1985 | IEEE Trans on PAS1985，104，1： | 1 |
| 15 | Hypertension following erythropoietin therapy in anemia hemodialysis patients显示文摘 | Eschbach JW Haley NR | 1990 | Am J Hypertens1990，3，5： | 1 |
| 16 | Kinetic analysis of the RNAi en- zyme complex 显示文摘 | Haley B Zamore P D | 2004 | Nat Struct Mol Biol2004，11，： | 1 |
| 17 | Multitrait least squares for quantitative trait loci detection 显示文摘 | Knott S A Haley S C | 2000 | Genetics2000，156，： | 1 |
| 18 | Urgent therapy for stroke,part I,pilot study of tissue plasminogen activator administered within 90 minutes显示文摘 | Brott TG Haley EC Levy DE | 1992 | Stroke1992，23，： | 1 |
| 19 | KIT mutations are common in incidental gastrointestinal stromal tumors one centimeter or less in size显示文摘 | Corless CL McGreevey L Haley A | 2002 | Am J Pathol2002，160，5： | 1 |
| 20 | Myths regarding NINDS rtPA Stroke Trial: setting the record straight显示文摘 | Haley EC Lewandowski C Tilley BC | 1997 | Ann Emerg Med1997，30，7： | 1 |
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