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| 1 | How is mRNA expression predictive for protein expression? A correlation study on human circulating monocytes显示文摘在学习 mRNA 表示的一个关键假设是它在蛋白质表示的预言是增进知识的。然而,仅仅有限的研究在酵母探索了 mRNA 蛋白质表示关联或人的纸巾和结果是相对不一致的。我们从 30 个无关的女人在刚孤立的人的传播单核白血球在 mRNA 蛋白质表情上执行了关联分析。为 71 基因的表示蛋白质被确定并且由结合的 2-D 电气泳动识别了集体 spectrometry。相应 mRNA 表达式被 Affymetrix 基因芯片确定。重要关联(r=0.235, P < 0.0001 ) 包括所有学习基因和所有样品为整个数据集被观察。关联在基因本体论的不同生物范畴变化了。例如,最高的关联以细胞的部件为细胞外的区域的基因被完成(r=0.643, P < 0.0001 ) 并且最低关联为规定的基因被获得(r=0.099, P=0.213 ) 以生物过程。在染色体,样品的一半证明为 71 基因的重要积极关联和重要关联在所有样品在平均 mRNA 和平均蛋白质表示层次之间被发现(r=0.296, P < 0.01 ) 。在学习组水平,然而,仅仅五学习基因越过所有样品有重要积极关联。我们的结果显示出在 mRNA 和蛋白质表示层次之间的全面积极关联。然而,中等、变化的关联建议那 mRNA 表情可能有时是有用的,但是远非当然在预言蛋白质表示层次完善。 | Yanfang Guo Peng Xiao Shufeng Lei Feiyan Deng Gary Guishan Xiao Yaozhong Liu Xiangding Chen Liming Li Shan Wu Yuan Chen Hui Jiang Lijun Tan Jingyun Xie Xuezhen Zhu Songping Liang Hongwen Deng | 2008 | Acta Biochimica et Biophysica Sinica2008，40，5： | 7 |
| 2 | Ablation of Akt2 and AMPKα2 rescues high fat diet-induced obesity and hepatic steatosis through Parkin-mediated mitophagy显示文摘Given the opposing effects of Akt and AMP-activated protein kinase(AMPK)on metabolic homeostasis,this study examined the effects of deletion of Akt2 and AMPKα2 on fat diet-induced hepatic steatosis.Akt2–Ampkα2 double knockout(DKO)mice were placed on high fat diet for 5 months.Glucose metabolism,energy homeostasis,cardiac function,lipid accumulation,and hepatic steatosis were examined.DKO mice were lean without anthropometric defects.High fat intake led to adiposity and decreased respiratory exchange ratio(RER)in wild-type(WT)mice,which were ablated in DKO but not Akt2^(-/-) and Ampkα2^(-/-) mice.High fat intake increased blood and hepatic triglycerides and cholesterol,promoted hepatic steatosis and injury in WT mice.These effects were eliminated in DKO but not Akt2^(-/-) and Ampkα2^(-/-) mice.Fat diet promoted fat accumulation,and enlarged adipocyte size,the effect was negated in DKO mice.Fat intake elevated fatty acid synthase(FAS),carbohydrate-responsive element-binding protein(CHREBP),sterol regulatory element-binding protein 1(SREBP1),peroxisome proliferator-activated receptor gamma coactivator 1-alpha(PGC-1α),peroxisome proliferator-activated receptor-α(PPARα),PPARγ,stearoyl-CoA desaturase 1(SCD-1),phosphoenolpyruvate carboxykinase(PEPCK),glucose 6-phosphatase(G6Pase),and diglyceride O-acyltransferase 1(DGAT1),the effect was absent in DKO but not Akt2^(-/-) and Ampkα2^(-/-) mice.Fat diet dampened mitophagy,promoted inflammation and phosphorylation of forkhead box protein O1(FoxO1)and AMPKα1(Ser^(485)),the effects were eradicated by DKO.Deletion of Parkin effectively nullified DKO-induced metabolic benefits against high fat intake.Liver samples from obese humans displayed lowered microtubule-associated proteins 1A/1B light chain 3B(LC3B),Pink1,Parkin,as well as enhanced phosphorylation of Akt,AMPK(Ser^(485)),and FoxO1,which were consolidated by RNA sequencing(RNAseq)and mass spectrometry analyses from rodent and human livers.These data suggest that concurrent deletion of Akt2 and AMPKα2 offers resilience to fat diet-induced obesity and hepatic steatosis,possibly through preservation of Parkin-mediated mitophagy and lipid metabolism. | Shuyi Wang Jun Tao Huaguo Chen Machender R.Kandadi Mingming Sun Haixia Xu Gary D.Lopaschuk Yan Lu Junmeng Zheng Hu Peng Jun Ren | 2021 | Acta Pharmaceutica Sinica B2021，11，11： | 4 |
| 3 | Rapid Detection of Wheat Blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology显示文摘Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and Bangladesh.Since MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is ineffective.To develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)pathotype.Using polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and Bangladesh.To test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR machine.Following this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 sequences.The activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)activity.We then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat plants.This novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field. | Houxiang Kang Ye Peng Kangyu Hua Yufei Deng Maria Bellizzi Dipali Rani Gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson Yilin Zhou Xueping Zhou Md Tofazzal Islam Guo-Liang Wang | 2021 | Engineering2021，7，9： | 4 |
| 4 | Discovering small molecules as Wnt inhibitors that promote heart regeneration and injury repair显示文摘There are intense interests in discovering pro regenerative medicine leads that can promote cardiac differentiation and regeneration,as well as repair damaged heart tissues.We have combined zebrafish embryo-based screens with cardiomyogenesis assays to discover selective small molecules that modulate heart development and regeneration with minimal adverse effects.Two related compounds with novel structures,named as Cardiomogen 1 and 2(CDMG1 and CDMG2),were identified for their capacity to promote myocardial hyperplasia through expansion of the cardiac progenitor cell population.We find that Cardiomogen acts as a Wnt inhibitor by targeting p-catenin and reducing Tcf/Lef-mediated transcription in cultured cells.CDMG treatment of amputated zebrafish hearts reduces nuclear p-catenin in injured heart tissue,increases cardiomyocyte(CM)proliferation,and expedites wound healing,thus accelerating cardiac muscle regeneration.Importantly,Cardiomogen can alleviate the functional deterioration of mammalian hearts after myocardial infarction.Injured hearts exposed to CDMG1 display increased newly formed CMs and reduced fibrotic scar tissue,which are in part attributable to the^-catenin reduction.Our findings indicate Cardiomogen as a Wnt inhibitor in enhancing injury-induced CM proliferation and heart regeneration,highlighting the values of embryo-based small molecule screens in discovery of effective and safe medicine leads. | Shuying Xie Wenbin Fu Guangju Yu Xueli Hu Kaa Seng Lai Xiangwen Peng Yating Zhou Xuejiao Zhu Ptamen Christov Leah Sawyer Terri T.Ni Gary A.Sulikowski Zhongzhou Yang Ethan Lee Chunyu Zeng Wei E.Wang Tao P.Zhong | 2020 | Journal of Molecular Cell Biology2020，12，1： | 4 |
| 5 | Functional analysis of tomato calmodulin gene family during fruit development and ripening显示文摘Calmodulin is a ubiquitous calcium sensor to recognize the different developmental and/or stimulus-triggered calcium changes and regulate plant growth and development.However,the function of calmodulin remains elusive for fleshy fruit development.We performed expression studies of a family of six calmodulin genes(SlCaMs)in tomato fruit.All calmodulins showed a double peak expression pattern.The first flat peak appeared at 10–30 days after anthesis,but their expression rapidly declined at mature green and breaker.Then a sharp and even higher peak came at turning/pink stages.Among six calmodulins,SlCaM1 had the highest expression during fruit enlargement,whereas SlCaM2 was the major calmodulin during fruit ripening.However,SlCaMs showed different patterns in three ripening mutants rin,Nor and Nr.In particular,at the stages corresponding to mature green and breaker,the expression levels of SlCaMs in those mutants were significantly higher than wild-type.Furthermore,SlCaMs,especially SlCaM2 were upregulated by ethylene.Transiently overexpressing SlCaM2 in mature green fruit delayed ripening,while reducing SlCaM2 expression accelerated ripening.Our results suggest that SlCaMs play double roles to regulate fruit ripening.Prior to the ethylene burst,the ethylene-independent repression of SlCaMs might be critical for fruit to initiate the ripening process.After the ethylene burst,SlCaMs could participate in the ethylene coordinated rapid ripening. | Tianbao Yang Hui Peng Gary R Bauchan | 2014 | Horticulture Research2014，1，1： | 3 |
| 6 | Effects of fruit thinning on ascorbate–glutathione cycle metabolism in black currants(Ribes nigrum L.)显示文摘To elucidate mechanisms regulating ascorbic acid(AsA) biosynthesis and accumulation in the fruit and leaves of black currants,As A and the activities of key enzymes in the ascorbate–glutathione(As A–GSH) cycle were measured from fruit set to fruit ripening during fruit thinning treatments of three common commercial black currant cultivars that differed in their As A levels:'Risager'(low),'Brodtrop'(medium) and 'Adelinia'(high).Treatments were 50% fruit reduction(50% of total fruit set) by hand,control was no thinning.Fruit thinning treatment significantly increased As A content in fruit of all three cultivars from weeks 2 to 8,significantly decreased As A content in leaves from weeks 3 to 8.Dehydroascorbate reductase and monodehydroascorbate reductase activities in fruit and leaves had a similar pattern,increasing during week 2,rose until they peaked in week 4.Ascorbate peroxidase activity in fruit in the thinning treatment was slightly lower than in the control.Fruit thinning was shown to be a good model to test As A biosynthesis regulation and accumulation in black currants.The results from our study provided strong evidence that As A–GSH cycle involved in As A synthesis and accumulation in fruit. | Dong Qin Lijuan Zhao Yu Gary Gao Fangxiao Li Shulei Li Junwei Huo Shuang Lou Peng Liu | 2017 | Journal of Forestry Research2017，28，5： | 3 |
| 7 | Observed permafrost thawing and disappearance near the altitudinal limit of permafrost in the Qilian Mountains显示文摘Permafrost degradation has been widely reported on the Tibetan Plateau(TP).However,directly observed evidence of permafrost thawing processes and degradation rates are very limited,although it is expected to be prevalent near the periphery of a permafrost area.Here,we report permafrost changes and disappearance in the Qilian Mountains(northeastern TP)based on three boreholes instrumented along a 100 m transect during 2014–2021.Our results show that permafrost has significantly degraded in the study area:the mean downward thawing rate from the permafrost table was about 0.16 m per year while the mean upward thawing rate from the permafrost base was about 0.23 m per year.We estimate the mean lateral degradation rate of permafrost in this area was∼4.14 m per year.More dramatically,the 1.5 m thick permafrost layer at one of the boreholes thawed completely between April of 2018 and December of 2019.Our results indicate that changes in climatic condition may have played only a limited role in controlling the active layer thickness in the vicinity of the altitudinal limit of permafrost;moisture content and soil conditions play key roles in site-specific permafrost thawing.This study provides new quantitative insights for understanding changes near the altitudinal limit of permafrost,and we suggest that land surface models or Earth system model studies of the lateral heat exchanges should be implemented in order to better represent permafrost thawing processes. | Wen SUN Tingjun ZHANG Gary D.CLOW Yan-Hua SUN Wen-Yu ZHAO Ben-Ben LIANG Cheng-Yan FAN Xiao-Qing PENG Bin CAO | 2022 | Advances in Climate Change Research2022，13，5： | 2 |
| 8 | MOF-Like 3D Graphene-Based Catalytic Membrane Fabricated by One-Step Laser Scribing for Robust Water Purification and Green Energy Production显示文摘Increasing both clean water and green energy demands for survival and development are the grand challenges of our age.Here,we successfully fabricate a novel multifunctional 3D graphene-based catalytic membrane(3D-GCM)with active metal nanoparticles(AMNs)loading for simultaneously obtaining the water purification and clean energy generation,via a“green”one-step laser scribing technology.The as-prepared 3D-GCM shows high porosity and uniform distribution with AMNs,which exhibits high permeated fluxes(over 100 L m^(−2) h^(−1))and versatile super-adsorption capacities for the removal of tricky organic pollutants from wastewater under ultra-low pressure-driving(0.1 bar).After adsorption saturating,the AMNs in 3D-GCM actuates the advanced oxidization process to self-clean the fouled membrane via the catalysis,and restores the adsorption capacity well for the next time membrane separation.Most importantly,the 3D-GCM with the welding of laser scribing overcomes the lateral shear force damaging during the long-term separation.Moreover,the 3D-GCM could emit plentiful of hot electrons from AMNs under light irradiation,realizing the membrane catalytic hydrolysis reactions for hydrogen energy generation.This“green”precision manufacturing with laser scribing technology provides a feasible technology to fabricate high-efficient and robust 3D-GCM microreactor in the tricky wastewater purification and sustainable clean energy production as well. | Xinyu Huang Liheng Li Shuaifei Zhao Lei Tong Zheng Li Zhuiri Peng Runfeng Lin Li Zhou Chang Peng Kan-Hao Xue Lijuan Chen Gary J.Cheng Zhu Xiong Lei Ye | 2022 | Nano-Micro Letters2022，14，11： | 2 |
| 9 | Systematic comparison of hydrogen production from fossil fuels and biomass resources显示文摘Fossil fuels are the main energy source to satisfy the worldwide energy demands.However,the energy demands are increasing and the supply of fossil fuels is decreasing,thus many countries are looking for other fuel sources.Differing from the traditional fuels,hydrogen is considered as one of the most promising energy sources due to its intrinsic features such as clean,efficient,safe and sustainable.Developing novel technologies for hydrogen production from renewable sources(such as biomass)becomes a core area for the investigation of hydrogen industry.Within this work,different pathways for hydrogen production including steam reforming,electrolysis,and biomass gasification have been systematically compared in terms of yield and cost.This comparison is unique since the systematic evaluation was conducted from many aspects for all the hydrogen production pathways,especially those by involving the biomass gasification that still lack of available literatures.The assessment methods involved energy analysis,exergy analysis and economic analysis.It was concluded that steam reforming remains the cheapest method of hydrogen production at 1.748$/kg,however,steam reforming is not an ideal process currently or for the future,gasification and electrolysis remains competitive with high yield but requires relatively high initial and annual expenditure.For biomass gasification,though its energy efficiency is lower than steam reforming,it has relatively higher mass yield,demonstrating the feasibility of this process for hydrogen production.Further for biomass gasification,the selection of correct feedstock is a key to maximize its yield,i.e.a yield of 82.47%is possible with corn stover fed gasification. | Kang Peng Gary Morrow Zhang Xiaolei Wang Tipeng Tan Zhongfu Jayant Agarwal | 2017 | International Journal of Agricultural and Biological Engineering2017，10，6： | 2 |
| 10 | Effect of Single Oral Doses of Sitagliptin, a Dipeptidyl Peptidase-4 Inhibitor, on Incretin and Plasma Glucose Levels after an Oral Glucose Tolerance Test in Patients with Type 2 Diabetes显示文摘 | Gary A. Herman Arthur Bergman Catherine Stevens Paul Kotey Bingming Yi Peng Zhao Bruno Dietrich George Golor Andreas Schrodter Bart Keymeulen Kenneth C. Lasseter Mark S. Kipnes Karen Snyder Deborah Hilliard Michael Tanen Caroline Cilissen Marina De Smet I | 2006 | The Journal of Clinical Endocrinology & Metabolism2006，，11： | 1 |
| 11 | Factors associated with the removal of fractured NiTi instruments from root canal systems显示文摘 | Ya Shen Bin Peng Gary Shun-pan Cheung | 2004 | Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology2004，，5： | 1 |
| 12 | Spray retention for liquid and mycoherbicide inoculum in three weed-biocontrol systems显示文摘 | Kelly N. Byer Gary Peng Thomas M. Wolf Brian C. Caldwell | 2006 | Biocontrol Science and Technology2006，，8： | 1 |
| 13 | The magnitude of global fresh-water transports of importance to ocean circulation显示文摘 | W S Broecker T H Peng Jean Jouzel Gary Russell | 1990 | Climate Dynamics1990，，2： | 1 |
| 14 | Incidence and Related Factors Associated with Postpreparation Pain after Intracanal Procedures in a Dental Teaching Hospital显示文摘目的:了解根管预备后疼痛发生率,并对一些可能与疼痛发生有关的因素进行分析。方法:选取2001年7月至2002年2月间武汉大学口腔医院牙体牙髓科完成的非一次法根管治疗纪录表,将相关信息输入计算机后作Chi-square test和logistic回归分析。结果:根管预备后疼痛发生率为11.5%。单因素分析和logistic回归发现身体状况、年龄、牙位于上颌或下颌、术前有无叩痛及牙髓活力为影响疼痛发生率的主要因素。结论:根管预备术后疼痛发生率较低;根管预备术后疼痛发生率受多种因素影响。 | Yue Cheng Gary Shun - Pan Cheung Zhuan Bian Bin Peng | 2008 | 口腔医学研究2008，24，2： | 1 |
| 15 | Laboratory Diagnosis and Monitoring the Viral Shedding of SARS-CoV-2 Infection显示文摘The worldwide epidemic of coronavirus disease 2019(COVID-19)is ongoing.Rapid and accurate detection of the causative virus SARSCoV-2 is vital for the treatment and control of COVID-19.In this study,the comparative sensitivity of different respiratory specimen types were retrospectively analyzed using 3,552 clinical samples from 410 COVID-19 patients confirmed by Guangdong CDC(Center for Disease Control and Prevention).Except for bronchoalveolar lavage fluid(BALF),the sputum possessed the highest positive rate(73.4%–87.5%),followed by nasal swabs(53.1%–85.3%)for both severe and mild cases during the first 14 days after illness onset(d.a.o.).Viral RNA could be detected in all BALF samples collected from the severe group within 14 d.a.o.and lasted up to 46 d.a.o.Moreover,although viral RNA was negative in the upper respiratory samples,it was also positive in BALF samples in most cases from the severe group during treatment.Notably,no viral RNA was detected in BALF samples from the mild group.Despite typical ground-glass opacity observed via computed tomographic scans,no viral RNA was detected in the first three or all upper respiratory tract specimens from some COVID-19 patients.In conclusion,sputum is most sensitive for routine laboratory diagnosis of COVID-19,followed by nasal swabs.Detection of viral RNA in BALF improves diagnostic accuracy in severe COVID-19 patients. | Yang Yang Minghui Yang Jing Yuan Fuxiang Wang Zhaoqin Wang Jinxiu Li Mingxia Zhang Jinli Wei Ling Peng Gary Wong Haixia Zheng Weibo Wu Chenguang Shen Mingfeng Liao Kai Feng Jianming Li Qianting Yang Juanjuan Zhao Lei Liu Yingxia Liu | 2020 | The Innovation2020，1，3： | 1 |
| 16 | A preliminary study on the effects of dietary supplementation of brewers yeast and nucleotides, singularly or in combination, on juven lie red drum ( Sciaenops ucellatus ) 显示文摘 | Peng Li Gary S Burr Jonathan Golf | 2005 | Aquaculture Re- search2005，36，11： | 1 |
| 17 | 在药品研发及商业化全程实施药品安全科学管理规范(三)显示文摘为系统阐述和研究药品安全科学,全面引入药品风险管理理念,国家药品不良反应监测中心特邀请国外相关专家共同撰写了《在药品研发及商业化全程实施药品安全科学管理规范》一文,并将其译成中文以共享。 | Kasia Petchel Rebecca Wang Gary Slatko Daniel Reshef Michael Peng 陈易新 | 2009 | 中国药物警戒2009，6，3： | 1 |
| 18 | 在药品研发及商业化全程实施药品安全科学管理规范(一)显示文摘为系统阐述和研究药品安全科学,全面引入药品风险管理理念,国家药品不良反应监测中心特邀请国外相关专家共同撰写了《在药品研发及商业化全程实施药品安全科学管理规范》一文,并将其译成中文以共享。 | Kasia Petchel Rebecca Wang Gary Slatko Daniel Reshef Michael Peng 陈易新 | 2009 | 中国药物警戒2009，6，1： | 0 |
| 19 | Author correction to‘Ablation of Akt2 and AMPKα2 rescues high fat diet-induced obesity and hepatic steatosis through Parkin-mediated mitophagy’[Acta Pharmaceutica Sinica B 11(2021)3508-3526]显示文摘The authors of the above-mentioned paper noticed an error in Fig.5a as reported.H&E staining was shown in wrong color during figure processing and DKO-LF group was accidently copied and pasted twice.Original H&E images have been replaced with the correct color with the accurate grouping information.No text content in figure legend or conclusion is affected by this error.The authors sincerely apologize for any inconvenience caused to the journal and readers. | Shuyi Wang Jun Tao Huaguo Chen Machender R.Kandadi Mingming Sun Haixia Xu Gary D.Lopaschuk Yan Lu Junmeng Zheng Hu Peng Jun Ren | 2023 | Acta Pharmaceutica Sinica B2023，13，2： | 0 |
| 20 | Suppression of LjBAK1-mediated immunity by SymRK promotes rhizobial infection in Lotus japonicus.显示文摘An important question in biology is how organisms can associate with different microbes that pose no threat (commensals), pose a severe threat (pathogens) and those that are beneficial (symbionts). The root nodule symbiosis serves as important model system to address such questions in the context of plant-microbe interactions. It is now generally accepted that rhizobia have the abilities to actively suppress host immune responses during the infection process, analogous to the way in which plant pathogens can evade immune recognition. However, much remains to be elucidated with regard to the mechanisms by which the host recognizes the rhizobia as pathogens and how, subsequently, these pathways are suppressed to allow establishment of the nitrogen fixing symbiosis. In this study, we found that SymRK (Symbiosis Receptor-like Kinase) is required for rhizobial suppression of plant innate immunity in Lotus japonicus. SymRK associates with LjBAK1 (BRASSINOSTEROID INSENSITIVE 1-Associated receptor Kinase 1), a well characterized, positive regulator of plant innate immunity, and directly inhibits LjBAK1 kinase activity. Rhizobial inoculation enhances the association between SymRK and LjBAK1 in planta. LjBAK1 is required to regulate plant innate immunity and plays a negative role in mediating rhizobial infection in L. japonicus. The data indicate that the protein complex of SymRK-LjBAK1 serves as an intersection point between rhizobial symbiotic signaling pathways and innate immunity pathways, which provides an evidence that rhizobia might actively suppress the host's ability to mount a defense response in the legume-rhizobium symbiosis. | Yong Feng Ping Wu Chao Liu Liwei Peng Tao Wang Chao Wang Qian Tan Bixuan Li Yajuan Ou Hui Zhu Songli Yuan Renliang Huang Gary Stacey Zhongming Zhang Yangrong Cao | 2021 | Molecular Plant2021，14，11： | 0 |
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