|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | How is mRNA expression predictive for protein expression? A correlation study on human circulating monocytes显示文摘在学习 mRNA 表示的一个关键假设是它在蛋白质表示的预言是增进知识的。然而,仅仅有限的研究在酵母探索了 mRNA 蛋白质表示关联或人的纸巾和结果是相对不一致的。我们从 30 个无关的女人在刚孤立的人的传播单核白血球在 mRNA 蛋白质表情上执行了关联分析。为 71 基因的表示蛋白质被确定并且由结合的 2-D 电气泳动识别了集体 spectrometry。相应 mRNA 表达式被 Affymetrix 基因芯片确定。重要关联(r=0.235, P < 0.0001 ) 包括所有学习基因和所有样品为整个数据集被观察。关联在基因本体论的不同生物范畴变化了。例如,最高的关联以细胞的部件为细胞外的区域的基因被完成(r=0.643, P < 0.0001 ) 并且最低关联为规定的基因被获得(r=0.099, P=0.213 ) 以生物过程。在染色体,样品的一半证明为 71 基因的重要积极关联和重要关联在所有样品在平均 mRNA 和平均蛋白质表示层次之间被发现(r=0.296, P < 0.01 ) 。在学习组水平,然而,仅仅五学习基因越过所有样品有重要积极关联。我们的结果显示出在 mRNA 和蛋白质表示层次之间的全面积极关联。然而,中等、变化的关联建议那 mRNA 表情可能有时是有用的,但是远非当然在预言蛋白质表示层次完善。 | Yanfang Guo Peng Xiao Shufeng Lei Feiyan Deng Gary Guishan Xiao Yaozhong Liu Xiangding Chen Liming Li Shan Wu Yuan Chen Hui Jiang Lijun Tan Jingyun Xie Xuezhen Zhu Songping Liang Hongwen Deng | 2008 | Acta Biochimica et Biophysica Sinica2008，40，5： | 7 |
| 2 | Dataset of the plasma membrane proteome of nasopharyngeal carcinoma cell line HNE1 for uncovering protein function显示文摘鼻咽的癌(NPC ) 是在南部的中国和东南亚洲的一个通常发生的肿瘤。当前的学习集中了于为 NPC 房间线 HNE1 的外部、不可分的蛋白质开发一个广泛的分析方法。外部膜蛋白质被 biotinylated 丰富提取, 0.1 M Na 2 公司 3, 和 H 2 O。不可分或全部的血浆膜部分用 30% Percoll 密度等级 centrifugation 被准备与或没有 0.1 M Na 2 公司 3 处理并且由西方的污点分析评估了。蛋白质受到与双人脚踏车团 spectrometry,与双人脚踏车团 spectrometry 相结合的钠 dodecyl sulfate-polyacrylamide 胶化电气泳动,和猎枪分析相结合的二维的电气泳动。我们分别地从外部、不可分、全部的血浆膜部分识别了 371, 180,和 702 蛋白质。总共, 848 非冗余的蛋白质(534 个组) 被识别。有约束力、催化、结构的分子是主要的班。除了 NPC 房间的已知的房间表面标记,分析在癌症房间是特征的疾病小径揭示了涉及多重发信号房间的小径的 311 蛋白质和 25 蛋白质。由寻找,差别表示了蛋白质数据库(http://gffzze0f623e48f094866h0wv5fn9wb0vn65qp.ffgz.tsg.suse.edu.cn/depd/index.jsp ) , 199 蛋白质被发现是在以前的癌症 proteome 研究表示的差别。671 蛋白质蛋白质相互作用网络被获得,包括在这个工作的 178 识别蛋白质。五蛋白质的血浆膜本地化被免疫学的技术证实,验证这 proteomic 策略。我们的学习能为理解 NPC 的分子的机制提供一些帮助。 | Lijun Zhang Xiaofang Jia Xiaohui Liu Tingting Sheng Rui Cao Quanyuan He Zhen Liu Xia Peng JixianXiong Pengfei Zhang Ni Shi Songping Liang | 2008 | Acta Biochimica et Biophysica Sinica2008，40，1： | 5 |
| 3 | Assignment of the three disulfide Bridges of huwentoxin-I, a neurotoxin from the spiderSelenocosmia huwena显示文摘 | Dongyi Zhang Songping Liang | 1993 | Journal of Protein Chemistry1993，，6： | 1 |
| 4 | Evaluation of strategy for analyzing mouse liver plasma membrane proteome显示文摘Plasma membrane (PM) proteome is one of the major subproteomes present in the cell,and is very important in liver function. In the present work, C57 mouse liver PM was purified by density-gradient centrifugation. The purified PM was verified by electron microscope analysis and Western blotting. The results showed that the PM was enriched by more than 20-fold and the contamination of mitochondria was reduced by 2-fold compared with the homogenization fraction. Proteins were separated by 2DE and 1DE, trypsin-digested and submitted to ESI-Q-TOF and MALDI-TOF-TOF mass spectrometry or directly digested in solution and analyzed by LC-ESI ion trap mass spectrometry. In all, 547 non-redundant mouse liver PM proteins were identified, of which 34% contributed to plasma membrane or plasma membrane-related proteins. This study optimized and evaluated the HLPP plasma membrane proteome analysis method and made a systematic analysis on PM proteome. | CHEN Ping ZHANG LiJun LI XuanWen WANG XiE CAO Rui LIU Zhen XIONG JiXian PENG Xia WEI YingJuan YING XingFeng WANG XianChun LIANG SongPing | 2007 | Science China(Life Sciences)2007，50，6： | 1 |
| 5 | Sample preparation for the analysis of membrane proteomes by mass spectrometry显示文摘The low abundance and highly hydrophobic nature of most membrane proteins make their analysis more difficult than that for common soluble proteins.Successful membrane protein identification is largely dependent on the sample preparation including the enrichment and dissolution of the membrane proteins.A series of conventional and newly developed methods has been applied to the enrichment of low-abundance membrane proteins at membrane and/or protein levels and to the dissolution of hydrophobic membrane proteins.However,all the existing methods have inherent advantages and limitations.Up to now,there has been no unique method that can universally be employed to solve all the problems and more efforts are needed in improving sample preparation for the analysis of membrane proteomes. | Xianchun Wang Songping Liang | 2012 | Protein & Cell2012，3，9： | 1 |
| 6 | Isolation and characterization of Jingzhaotoxin-V, a novel neurotoxin from the venom of the spider Chilobrachys jingzhao显示文摘 | Xiongzhi Zeng Meichun Deng Ying Lin Chunhua Yuan Jianhui Pi Songping Liang | 2007 | Toxicon2007，49，3： | 1 |
| 7 | De nove sequencing of tryptic peptides sulfonated by 4-sulfophenyl isothiocyanate for unambiguous protein identification using post-source decay matrix-assisted laser desorption/ionization mass spectrometry显示文摘 | Chen Ping Nie Song Wang Xiangchun Liang Songping | 2004 | Rapid Commun Mass Spectrom2004，18，： | 1 |
| 8 | Proteomic and peptidomic analysis of the venom from Chinese tarantula Chilobrachys jingzhao显示文摘 | Zhi Liao Jia Cao Shuiming Li Xiaojun Yan Weijun Hu Quanyuan He Jinjun Chen Jianzhou Tang Jinyun Xie and Songping Liang | 2007 | Proteomics2007，，7： | 1 |
| 9 | Differential protein expression in low-grade astrocytomas and peritumoral human brain tissues显示文摘Differential protein expression between various pathological grades of glioma has been shown in studies of glioma proteomics. However,very little data is available regarding normal brain tissues and glioma differential protein expression,because normal human brain tissues are difficult to harvest. The present study selected samples from low-grade astrocytomas and peritumoral brain tissues to analyze differential protein expression by two-dimensional(2D) electrophoresis and mass spectrometry techniques. Results revealing 36 protein spots by 2D electrophoresis,including 23 spots revealing increased expression and 13 spots revealing decreased expression. However,25 differential proteins were identified by mass spectrometry,including 16 proteins with increased expression and 9 with decreased expression. Western blot analysis confirmed the mass spectrometry results,i.e.,heat shock protein 70(HSP70) and human transthyretin(TTR) expressions were increased,but glial fibrillary acidic protein(GFAP) was decreased,in astrocytomas. The present study constructed a 2D electrophoresis pattern between low-grade astrocytomas in the human brain and peritumoral tissues. Results demonstrated that a majority of differential proteins,such as HSP70,TTR,and GFAP,participate in malignant progression of gliomas. | Guangzhong Xiong Huisheng Xiao Junxian Lu Dongshan Zhang Changlong Bi Li Peng Ge Xiong Li Xiong Ping Chen Songping Liang | 2010 | Neural Regeneration Research2010，5，24： | 0 |
| 10 | Effects of Arg26 and Lys27 mutation on the bioactivity of HNTX-Ⅳ显示文摘Hainantoxin-Ⅳ(HNTX-Ⅳ)was isolated from the Chinese bird spider Ornithoctorcs hainana and identified as a novel antagonist of tetrodotoxin-sensitive(TTX-S)sodium channels.As revealed by the solution structure of HNTX-Ⅳ solved by two-dimensional nuclear magnetic resonance(2D-NMR),HNTX-Ⅳ adopts an inhibitor cystine knot motif.To check the role of basic residues during HNTX-Ⅳ’s interaction with TTX-S sodium channels,R26A and K27A mutants of HNTX-Ⅳ were constructed by solid-phase chemical synthesis.The synthesized peptides were purified and refolded under optimized oxidation conditions.Correct synthesis and folding were confirmed by MALDI-TOF mass spectrometry and NMR spectroscopy,respectively.Using the whole-cell patch-clamp technique,Lys27 but not Arg26 was identified as a key residue for HNTX-Ⅳ’s bioactivity against TTX-S sodium channels,because R26A-HNTX-Ⅳ showed slightly reduced activity and K27A-HNTX-Ⅳ showed almost no inhibition. | XIONG Xia XU Xia LI Dongling CHEN Ping LIANG Songping | 2007 | Frontiers in Biology2007，2，1： | 0 |
| 11 | Molecular diversification of antimicrobial peptides from the wolf spider Lycosa sinensis venom based on peptidomic,transcriptomic,and bioinformatic analyses显示文摘The venom of Lycosoidea spiders is a complex multicomponent mixture of neurotoxic pep-tides(main components)and antimicrobial peptides(AMPs)as minor components.In this study,we described the high-throughput identification and analysis of AMPs from Lycosa sinensis venom(named LS-AMPs)using a combination strategy that includes the following three differ-ent analysis approaches:(i)peptidomic analysis,namely reversed-phase high-performance liquid chromatography(RP-HPLC)separation plus top-down sequencing by matrix-assisted laser desorp-tion/ionization time-of-flight mass spectrometry(MS);(i)transcriptomic analysis,namely cDNA library construction plus DNA sequencing;(ii)i)bioinformatic analysis,namely analysis and predic-tion for molecular characters of LS-AMPs by the online biology databases.In total,52 sequences of AMPs were identified from L.sinensis venom,and all AMPs can be categorized into eight different families according to phylogenetic analysis and sequence identity.This is the largest number of AMPs identified from a spider species so far.In the present study,we demonstrated molecular char-acteristics,such as complex precursor,N-and/or C-terminally truncated analogs,and C-terminal amidation of LS-AMPs from L.sinensis venom.This is a preliminary investigation on the molecu-lar diversification of venom-derived AMPs from the wolf spider species(family Lycosidae),and a detailed investigation on the functional diversity of LS-AMPs will be preformed in the future. | Xing Tang Jing Yang Zhigui Duan Liping Jiang Zhonghua Liu Songping Liang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，11： | 0 |
| 12 | Impacts of environmental and canopy conditions on the nighttime sap flow of larch plantations in the Liupan Mountains,China显示文摘Nighttime sap flow(Q_(n))is an important physiological activity under which trees manage drought stress.An in-depth understanding of the characteristics of Q_(n)and its response to environmental and canopy conditions are of significance for arid area forest and water management.This study measured daily sap flow(Q_(s))of a Larix principis-rupprechtii plantation in the Liupan Mountains,northwest China during the 2017-2019 growing seasons,and separated Q_(s)into daytime sap flow(Qd)and Q_(n).Meteorological conditions(reference evapotranspiration,ETref),canopy structure(leaf area index,LAI),and soil moisture(relative soil water content,RSWC)were considered as the main biophysical factors affecting Q_(n).The structural equation model and upper boundary line method determined the effects of compound and single factors on Q_(n)The daily mean Q_(n)values during the growing seasons in 2017,2018,and 2019 were 0.024,0.026,and 0.030 mm d-1,accounting for 6.2,11.2,and 10.1%of Q_(s),respectively.Q_(n)at different canopy development phases(leaf expanding,LG;leaf expanded,LD;and defoliation,DF)over three years was LD>LG>DF.Q_(n)increased with increasing ETref,whereas the ratio of Q_(n)to Q_(s)decreased.Q_(n)did not show regular variation in the three-year growing seasons under different soil moisture conditions.ETrefand LAI mainly controlled Q_(n)by affecting Qd,whereas RSWC had no significant effect on Q_(n).Q_(n)had a positive and linear relationship with LAI and a quadratic relationship with ETref.Both explained 40%of variation in Q_(n)Meteorological and canopy conditions are important factors affecting Q_(n)on the semi-humid study site.The application of the Q_(n)model coupled with the impact of ETrefand LAI furthers understanding of the impacts of climate and forest structure change on Q_(n). | Songping Yu Jianbin Guo Zebin Liu Yanhui Wang Lihong Xu Pengtao Yu Liang He | 2023 | Journal of Forestry Research2023，34，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员