|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Establishment and Optimization of SRAP Amplification System in Lonicara caerulea L.显示文摘A single factor design was applied to optimize five factors influencing SRAP system,including Taq DNA polymerase,template DNA concentration,dNTPs,primer and Mg 2+,each at four levels. The optimal SRAP-PCR system for Lonicera caerulea L. was 20 μL SRAP-PCR amplification reaction solution containing 2.0 μL 10×PCR buffer,1.0 U Taq DNA polymerase,30 ng template DNA,0.2 mmol L -1 dNTPs,2.0 mmol L -1 Mg 2+ and 0.2 μmol L -1 primer. The suitable amplification procedure consisted of an initial denaturation at 94℃ for 5 min;denaturation at 94℃ for 1 min,annealing at 35℃ for 1 min,extension at 72℃ for 90 s and in total five cycles;denaturation at 94℃ for 1 min,annealing at 50℃ for 1 min,extension at 72℃ for 90 s and in total 35 cycles;extension at 72℃ for 8 min;preservation at 4℃. The procedures and systems could meet the demand for SRAP amplification of Lonicera caerulea L. and would play an important role in Lonicera caerulea L. germplasm identification and genetic diversity analysis. | SUN Feng HUO Junwei QIN Dong | 2011 | Journal of Northeast Agricultural University(English Edition)2011，18，4： | 4 |
| 2 | Effects of fruit thinning on ascorbate–glutathione cycle metabolism in black currants(Ribes nigrum L.)显示文摘To elucidate mechanisms regulating ascorbic acid(AsA) biosynthesis and accumulation in the fruit and leaves of black currants,As A and the activities of key enzymes in the ascorbate–glutathione(As A–GSH) cycle were measured from fruit set to fruit ripening during fruit thinning treatments of three common commercial black currant cultivars that differed in their As A levels:'Risager'(low),'Brodtrop'(medium) and 'Adelinia'(high).Treatments were 50% fruit reduction(50% of total fruit set) by hand,control was no thinning.Fruit thinning treatment significantly increased As A content in fruit of all three cultivars from weeks 2 to 8,significantly decreased As A content in leaves from weeks 3 to 8.Dehydroascorbate reductase and monodehydroascorbate reductase activities in fruit and leaves had a similar pattern,increasing during week 2,rose until they peaked in week 4.Ascorbate peroxidase activity in fruit in the thinning treatment was slightly lower than in the control.Fruit thinning was shown to be a good model to test As A biosynthesis regulation and accumulation in black currants.The results from our study provided strong evidence that As A–GSH cycle involved in As A synthesis and accumulation in fruit. | Dong Qin Lijuan Zhao Yu Gary Gao Fangxiao Li Shulei Li Junwei Huo Shuang Lou Peng Liu | 2017 | Journal of Forestry Research2017，28，5： | 3 |
| 3 | Effects of GA3 and ABA on the respiratory pathways during the secondary bud burst in black currants显示文摘The effects of exogenous gibberellic acid(GA_3)and abscisic acid(ABA) on the total respiratory rate, percentages of total respiratory rate contributed by respiratory pathways [Embden-Meyerhof-Parnas Pathway(EMP),Pentose Phosphate Pathway(PPP), and Tricarboxylic Acid Cycle(TCA)], and conversion of starch to soluble sugars in the buds of black currants during secondary bud burst were investigated to determine the relationship between respiratory rates and secondary bud burst. ‘Adelinia', a black currant cultivar that is prone to secondary bud burst after the first harvest, was used in this study. Mature bushes of Adelinia were sprayed with 30 mg/L GA_3 and 50 mg/L ABA to manipulate bud burst. The results showed that exogenous applications of GA_3 and ABA had opposite effects on bud respiratory rate. Generally, GA_3 treatment increased the total respiratory rate and respiratory rate of the TCA and PPP, and the respiratory rates after GA_3 treatment were higher than those of control. While ABA treatment mostly decreased the total respiratory rate and the respiratory rate of TCA and PPP in buds in comparison to control. In terms of the percentage of the three respiratory rates in comparison to the total respiratory rate, GA_3 treatment significantly increased the percentage of TCA and PPP respiratory rate in comparison to the control(P \ 0.01), whereas ABA decreased the rates. GA_3 significantly increased the content of soluble sugars and decreased the starch content, while the starch content in buds after ABA treatment was significantly higher than that of the control. All results showed that PPP is a critical process for the second bud burst in black currants. While the EMP–TCA pathway is still dominant in bud respiration,provides a series of basic materials and energy(ATP). The conversion of starch to soluble sugars is essential for bud burst. Thus, we conclude that an energy shortage is a main contributor in ABA inhibition of the secondary bud burst of black currants. | Dong Qin Huanhuan Wang Chunhao Zhang Zeyuan Yu Yu Gary Gao Fuchun Xie Ning Hung Junwei Huo | 2017 | Journal of Forestry Research2017，28，4： | 2 |
| 4 | Regeneration of Blue Honeysuckle via Dormant Axillary Buds显示文摘The optimum medium for dormant axillary buds culture of blue honeysuckle was screened according to the growth rate and elongation rate by inoculating the buds on culture medium with various 6-BA and iron-salt concentration.About 35 days,the stretched stem buds were divided into strong root system after inoculated on 1/2 MS+1.0 mg · L-1 IBA rooting medium.Amount of qualified tissue-cultured young plants could be obtained by the stretched stem buds reproduction. | QU Guiqin HUANG Longshuang HUO Junwei | 2008 | Journal of Northeast Agricultural University(English Edition)2008，15，2： | 1 |
| 5 | Microstructure and Mechanical Properties of an Ultrahigh-strength Titanium alloy Ti-4.5Al-5Mo-5V-6Cr-1Nb Prepared Using Laser Directed Energy Deposition and Forging:A Comparative Study显示文摘The application of titanium alloys in aerospace put forward the requirement for higher strength.Additive manu-facturing is a promising method for the efficient and economical processing of titanium alloys.However,research on the additive manufacturing of ultrahigh-strength titanium alloys is still limited.The mechanisms of microseg-regation for high alloying elements and poor plasticity are still not clear.In this study,an ultrahigh-strength titanium alloy Ti-4.5Al-5Mo-5V-6Cr-1Nb(TB18)was prepared using two methods:laser direct energy deposi-tion(LDED)and forging.The LDEDed alloy contains three zones with similar grain morphologies but different microstructure.The microsegregation of the alloy is limited due to the rapid solidification and almost eliminated after the thermal cycle and solution treatment.With stress relief treatment,the LDEDed alloy exhibits anisotropic mechanical properties.After solution and aging treatments,its ultimate strength is enhanced;however,its plas-ticity is relatively lower than that of the wrought alloy with equally high strength.The excellent balance of the strength and plasticity of the wrought alloy can be ascribed to the formation of��WGB and multiscale��laths,which provides enlightenment for optimizing the properties of the LDEDed alloy. | Junwei Yang Haibo Tang Peiyuan Wei Hongwei Gao Jiawei Wang Haixin Huo Yanyan Zhu | 2023 | Chinese Journal of Mechanical Engineering(Additive Manufacturing Frontiers)2023，2，1： | 0 |
| 6 | Three-dimensional boron nitride network/polyvinyl alcohol composite hydrogel with solid-liquid interpenetrating heat conduction network for thermal management显示文摘Polyvinyl alcohol hydrogels have been used in wearable devices due to their good flexibility and biocompatibility.However,due to the low thermal conductivity(κ)of pure hydrogel,its further application in high power devices is limited.To solve this problem,melamine sponge(MS)was used as the skeleton to wrap boron nitride nanosheets(BNNS)through repeated layering assembly,successfully preparing a three-dimensional(3D)boron nitride network(BNNS@MS),and PVA hydrogels were formed in the pores of the network.Due to the existence of the continuous phonon conduction network,the BNNS@MS/PVA exhibited an improvedκ.When the content of BNNS is about 6 wt.%,κof the hydrogel was increased to 1.12 W m^(-1)K^(-1),about two times higher than that of pure hydrogel.The solid heat conduction network and liquid convection network cooperate to achieve good thermal management ability.Combined with its high specific heat capacity,the composites have an important application prospect in the field of wearable flexible electronic thermal management. | Mengmeng Qin Yajie Huo Guoying Han Junwei Yue Xueying Zhou Yiyu Feng Wei Feng | 2022 | Journal of Materials Science & Technology2022，，32： | 0 |
| 7 | Transient Expression of BVDV N^(pro) Gene in vitro and Distribution of Fusion Protein in Cells显示文摘The gene encoding nonstructural protein Npro of BVDV was amplified by PCR,then was inserted into pEGFP-C1 vector.The 293 cells were transfected in vitro with recombinant plasmid by liposome.Npro-EGFP fusion protein was viewed directly with fluorensce microscope and mRNA expression of Npro was detected by reverse transcription-polymerase chain reaction(RT-PCR).Results showed that recombinant plasmid was confirmed to be constructed correctly by PCR,restriction enzyme digestion and DNA sequencing;meanwhile,the gene carried was expressed in 293 cells.The fusion protein had properties of both the two Npro and enhanced green fluorescent proteins(EGFP) and distributed in the cytoplasm.This research lays a foundation for further researches which explored Npro gene function in the process of viral replication and synthesis. | HUO Hui WU Mingfu WANG Junwei | 2011 | Journal of Northeast Agricultural University(English Edition)2011，18，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员