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| 1 | Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice显示文摘Nucleotide-binding leucine-rich repeat(NLR)proteins play critical roles in plant immunity.However,how NLRs are regulated and activate defense signaling is not fully understood.The rice(Oryza sativa)NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10(APIP10)negatively regulates Piz-t accumulation.In this study,we found that APIP10 interacts with two rice transcription factors,VASCULAR PLANT ONEZINC FINGER 1(OsVOZ1)and OsVOZ2,and promotes their degradation through the 26S proteasome pathway.OsVOZ1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in planta.The osvoz1 and osvoz2 single mutants display modest but opposite M.oryzae resistance in the non-Piz-t background.However,the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death,and silencing of both genes via RNA interference also leads to dwarfism,mild cell death,and enhanced resistance to M.oryzae in the non-Piz-t background.Both OsVOZ1 and OsVOZ2 interact with Piz-t.Double silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription,reactive oxygen species-dependent cell death,and resistance to M.oryzae containing AvrPiz-t.Taken together,these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity. | Jiyang Wang Ruyi Wang Hong Fang Chongyang Zhang Fan Zhang Zeyun Hao Xiaoman You Xuetao Shi Chan Ho Park Kangyu Hua Feng He Maria Bellizzi Kieu Thi Xuan Vo Jong-Seong Jeon Yuese Ning Guo-Liang Wang | 2021 | Molecular Plant2021，14，2： | 8 |
| 2 | PAPP2C Interacts with the Atypical Disease Resistance Protein RPW8.2 and Negatively Regulates Salicylic Acid-Dependent Defense Responses in Arabidopsis显示文摘真菌的许多和 oomycete 病原体区分说出的喂的结构从植物提取营养的 haustorium 表皮的房间。不正常的抵抗(R) 蛋白质 RPW8.2 激活水杨酸酸(SA )-dependent, 对 Golovinomyces spp 的指向 haustorium 的防卫,多重植物种类上的粉的霉疾病的原因的代理人。RPW8.2 怎么激活防卫,仍然是 uncharacterized。这里,我们报导 RPW8.2 在酵母并且在由 co-immunoprecipitation 和双分子的荧光互补试金证实了的 planta 与联系 phytochrome 的蛋白质磷酸酶类型 2C (PAPP2C ) 交往。由在缺乏 RPW8.2 的 Col-0 植物的 RNA 干扰(RNAi ) 的 PAPP2C 的下面规定导致叶自发的细胞死亡和提高的疾病抵抗到经由 SA 依赖的发信号小径的粉的霉。而且,由在在强壮的象 HR 一样房间死亡的 RPW8.2 背景结果的 RNAi 的 PAPP2C 的下面规定,它相关与提高了 RPW8.2 表示。我们进一步表明那红血球凝聚素(哈) 从短暂地与 HAPAPP2C 转变的烟草叶房间准备的标注 PAPP2C 拥有磷酸酶活动。另外, silencing 米饭基因(Os04g0452000 ) 对相应 PAPP2C 也在米饭导致自发的房间死亡。联合,我们的结果建议 RPW8.2 机能上地与 PAPP2C 被连接并且 PAPP2C 否定地在 Arabidopsis 对粉的霉调整 SA 依赖的基础防卫。 | Wen-Ming Wang Xian-Feng Ma Yi Zhang Ming-Cheng Luo Guo-Liang Wang Maria Bellizzi Xing-Yao Xiong Shun-Yuan Xiao | 2012 | Molecular Plant2012，5，5： | 6 |
| 3 | Rapid Detection of Wheat Blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology显示文摘Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and Bangladesh.Since MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is ineffective.To develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)pathotype.Using polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and Bangladesh.To test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR machine.Following this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 sequences.The activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)activity.We then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat plants.This novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field. | Houxiang Kang Ye Peng Kangyu Hua Yufei Deng Maria Bellizzi Dipali Rani Gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson Yilin Zhou Xueping Zhou Md Tofazzal Islam Guo-Liang Wang | 2021 | Engineering2021，7，9： | 4 |
| 4 | Construction and Application of Efficient Ac-Ds Transposon Tagging Vectors in Rice显示文摘Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have constructed a series of transposon tagging vectors based on three approaches:(i) AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain (GVG) chemical-inducible expression system; (ii) deletion of AcTPase via Cre-lox site-specific recombination that was initially triggered by Ds excision; and (iii) suppression of early transposition events in transformed rice callus through a dual-functional hygromycin resistance gene in a novel Ds element (HPT-Ds). We tested these vectors in transgenic rice and characterized the transposition events. Our results showed that these vectors are useful resources for functional genomics of rice and other crop plants. The vectors are freely available for the community. | Shaohong Qu Jong-Seong Jeont Pieter B.F. Ouwerkerk Maria Bellizzi Jan Leach Pamela Ronald Guo-Liang Wang | 2009 | Journal of Integrative Plant Biology2009，51，11： | 0 |
| 5 | 水稻通过抑制稻瘟菌效应蛋白引起的细胞坏死抵御稻瘟菌的侵染显示文摘由稻瘟菌引起的稻瘟病是水稻生产中最严重的真菌性病害。然而,稻瘟菌如何引起水稻细胞坏死,以及水稻如何抑制稻瘟菌从活体营养阶段转换到死体营养阶段的分子机制还不清楚。 | Ruyi Wang Yuese Ning Xuetao Shi Feng He Chongyang Zhang Jiangbo Fan Nan Jiang Yu Zhang Ting Zhang Yajun Hu Maria Bellizzi 王国梁 | 2017 | 科学新闻2017，19，4： | 0 |
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