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| 1 | The antitumor effect of tanshinone IIA on antiproliferation and decreasing VEGF/VEGFR2 expression on the human non-small cell lung cancer A549 cell line显示文摘The effects of tanshinone IIA on the proliferation of the human non-small cell lung cancer cell line A549 and its possible mechanism on the VEGF/VEGFR signal pathway were investigated.The exploration of the interaction between tanshinone IIA and its target proteins provides a feasible platform for studying the anticancer mechanism of active components of herbs.The CCK-8 assay was used to evaluate the proliferative activity of A549 cells treated with tanshinone IIA(2.5 80 μmol/L) for 24,48 and 72 h,respectively.Flow cytometry was used for the detection of cell apoptosis and cell cycle perturbation.VEGF and VEGFR2 expression were studied by Western blotting.The binding mode of tanshinone IIA within thecrystal structure of the VEGFR2 protein was evaluated with molecular docking analysis by use of the CDOCKER algorithm in Discovery Studio 2.1.The CCK-8 results showed that tanshinone IIA can significantly inhibit A549 cell proliferation in a dose-and time-dependent manner.Flow cytometry results showed that the apoptosis rate of tested group was higher than the vehicle control,and tanshinone IIAtreated cells accumulated at the S phase,which was higher than the vehicle control.Furthermore,the expression of VEGF and VEGFR2 was decreased in Western blot.Finally,molecular docking analysis revealed that tanshinone IIA could be stably docked into the kinase domain of VEGFR2 protein with its unique modes to form H-bonds with Cys917 and π–π stacking interactions with Val848.In conclusion,tanshinone IIA may suppress A549 proliferation,induce apoptosis and cell cycle arrest at the S phase.This drug may suppress angiogenesis by targeting the protein kinase domains of VEGF/VEGFR2. | Jun Xie Jiahui Liu Heng Liu Shihui Liang Meigui Lin Yueyu Gu Taoli Liu Dongmei Wang Hui Gee Sui-lin Mo | 2015 | Acta Pharmaceutica Sinica B2015，5，6： | 26 |
| 2 | 无创机械通气时单用右美托咪定持续静点镇静的经典用法对血流动力学的影响以及撤药风险显示文摘目的右美托咪定用于儿童重症监护逐渐增加,其作为单一持续镇静药物时的长期效应尚无明确研究。本研究旨在了解长时间应用右美托咪定为唯一持续镇静药物时,其对血流动力学的影响、撤药策略及戒断反应风险因素。设计回顾性病历分析。场所大型单中心四级儿科医学院。对象共纳入382例患儿;〈18岁且于PICU内无创机械通气(non—invasive ventilation,NIPPV)期间仅静点右美托咪定镇静〉24h。干预措施记录右美托咪定常规用药方法。撤药策略分为:骤停,逐渐减停及改为口服可乐定。 | Shutes BL Gee SW Sargel CL 方伯梁 钱素云 | 2018 | 中国小儿急救医学2018，25，3： | 18 |
| 3 | Inflammatory bowel disease imaging:current practice andfuture directions显示文摘The purpose of this paper is to evaluate the role of imaging in inflammatory bowel disease(IBD), including detection of extraluminal complications and extraintestinal manifestations of IBD, assessment of disease activity and treatment response, and discrimination of inflammatory from fibrotic strictures. IBD is a chronic idiopathic disease affecting the gastrointestinal tract that is comprised of two separate, but related intestinal disorders; Crohn's disease and ulcerative colitis. The paper discusses, in detail the pros and cons of the different IBD imaging modalities that need to be considered in order to optimize the imaging and clinical evaluation of patients with IBD. Historically, IBD evaluation of the bowel has included imaging to assess the portions of the small bowel that are inaccessible to optical endoscopic visualization. This traditionally was performed using barium fluoroscopic techniques; however, cross-sectional imaging techniques(computed tomography and magnetic resonance imaging) are being increasingly utilized for IBD evaluation because they can simultaneously assess mural and extramural IBD manifestations. Recent advances in imaging technology, that continue to improve the ability of imaging to noninvasively follow disease activity and treatment response, are also discussed. This review article summarizes the current imaging approach in inflammatory bowel disease as well as the role of emerging imaging modalities. | Aoife Kilcoyne Jess L Kaplan Michael S Gee | 2016 | World Journal of Gastroenterology2016，22，3： | 8 |
| 4 | 钠-葡萄糖共转运蛋白-2抑制剂或胰高血糖素样肽-1受体激动剂治疗成人2型糖尿病:临床实践指南显示文摘临床问题对于存在不同心血管风险及肾脏结局的2型糖尿病患者,在原有生活方式干预和/或其他降糖药物的基础上加用钠-葡萄糖共转运蛋白2(SGLT-2)抑制剂和胰高血糖素样肽1(GLP-1)受体激动剂的获益及风险是什么?现行做法几十年来,2型糖尿病的治疗决策都以控制血糖为主导。SGLT-2抑制剂和GLP-1受体激动剂在传统观念中常被用于二甲双胍治疗后血糖仍控制不佳的患者。目前这一现状已经发生了改变,这得益于多项临床研究结果。研究显示SGLT-2抑制剂和GLP-1受体激动剂拥有独立于药物降糖作用之外的对于动脉粥样硬化性心血管病(CVD)和慢性肾脏病(CKD)的获益。建议本指南阐述了针对不同风险分层的成人2型糖尿病患者使用SGLT-2抑制剂或GLP-1受体激动剂的建议。•伴有3种或更少的心血管风险因素且不存在CVD或CKD:不建议启动SGLT-2抑制剂或GLP-1受体激动剂治疗。(推荐等级:弱)•伴有3种以上心血管风险因素且不存在CVD或CKD:建议启动SGLT-2抑制剂治疗,不建议启动GLP-1受体激动剂治疗。(推荐等级:弱)•已经存在CVD或CKD:建议启动SGLT-2抑制剂治疗和GLP-1受体激动剂治疗。(推荐等级:弱)•已经存在CVD和CKD:建议启动SGLT-2抑制剂治疗(推荐等级:强)和GLP-1受体激动剂治疗。(推荐等级:弱)•对于那些想要进一步降低CVD和CKD结局风险的患者:推荐优先启用SGLT-2抑制剂治疗而非GLP-1受体激动剂治疗。(推荐等级:弱)这项指南是如何制订的一个由患者、临床医生和方法学家共同组成的国际小组提出了这些推荐意见。这些推荐意见基于可信度较高的指南的标准,并使用GRADE分级方法进行评估。该小组采用了息者个体化的观点。证据一项关于获益与风险的系统综述和网络meta分析(764项随机对照研究,包括421346例参与者)发现SGLT-2抑制剂和GLP-1受体激动剂可以降低总体死亡率、心肌梗死发生率、终末期肾病或肾衰竭的发生率(中等至高等质量的证据)。在不同的亚组中这些药物对卒中、因心力衰竭所致住院和其他主要不良事件有不同的影响。药物绝对获益的程度因患者个体风险的不同有很大的差异。(例如,对于接受了超过5年药物治疗的1000例患者,在最低风险人群中死亡人数减少了5人,在最高风险人群中死亡人数减少了48人)。一项关于预后的综述确认了14种风险预测模型,其中一种(RECODe)在证据总结中报告了大部分基线风险评估数据,小组利用该模型以支持风险分层的建议。考虑到患者的价值观及个体差异,指南推荐的支撑证据包括一项对已发表论文的系统综述、一项患者焦点小组研究、一项临床问题总结,以及一项指南调查。指南解读我们依据不同的CVD和CKD风险水平,综合考虑获益、风险和其他因素的平衡,以及每一个风险组别的实际问题,来对推荐意见进行分层。本指南强烈建议CVD和CKD患者使用SGLT-2抑制剂治疗,这说明专家组认为其具有显著的获益。而对于其他成人2型糖尿病患者,推荐等级较弱,这说明专家组想要在获益、风险及治疗花费上取得一个更好的平衡。临床医生通过该指南可以使用可靠的风险计算模型,如RECODe,来明确其患者的个体心血管和肾脏疾病风险。医患交互式总结临床证据和制订决策有助于患者知晓治疗选择,包括进行共同决策。2型糖尿病人群(全球患病率不断增长1-2)正面临着不断增加的心血管疾病、肾脏病和其他并发症的风险3。数十年来,2型糖尿病的管理始终以控制血糖及糖化血红蛋白(HbA1c)为治疗目标4-5,但是,最近的高质量随机对照研究已经对这种以血糖为中心的治疗模式发起了挑战。研究结果显示,强化血糖控制未必会降低大血管不良事件,它还可能带来不利影响监管机构现在要求新型糖尿病药物必须证明其具有心血管和肾脏获益才能获得批准。对两类新药--钠-葡萄糖共转运蛋白2(SGLT-2)抑制剂和胰高血糖素样肽1(GLP-1)受体激动剂(见框图1)的临床试验结果显示,在现有治疗方案(常规治疗)之上加用这些药物,对死亡、心肌梗死、卒中、心力衰竭和肾脏的结局(如进展为终末期肾病)都有获益8-12。 | Sheyu Li Per Olav Vandvik Lyubov Lytvyn Gordon H Guyatt Suetonia C Palmer Rene Rodriguez-Gutierrez Farid Foroutan Thomas Agoritsas Reed A C Siemieniuk Michael Walsh Lawrie Frere David J Tunnicliffe Evi V Nagler Veena Manja Bjφrn Olav Asvold Vivekanand Jha Mieke Vermandere Karim Gariani Qian Zhao Yan Ren Emma Jane Cartwright Patrick Gee Alan Wickes Linda Fems Robin Wright Ling Li Qiukui Hao Reem A Mustafa 无 郭鹤鸣(译) | 2021 | 英国医学杂志中文版2021，24，9： | 7 |
| 5 | Grain size effect on wear resistance of WC-Co cemented carbides under different tribological conditions显示文摘The grain-size dependence of wear resistance of WC-Co cemented carbides(with mean WC grain sizes of 2.2μm,1.6μm,0.8μm and 0.4μm,respectively)was investigated under different tribological conditions.The results showed that the grain size had opposite effects on wear resistance of the cemented carbides in dry sliding wear and microabrasion tests.In the former condition,with decrease of WC grain size hence the increase of hardness,plastic deformation,fracture,fragmentation and oxidation were all mitigated,leading to a drastic decrease in the wear rate.In the latter condition,pull-out of WC grains after Co removal dominated the wear,so that the hardness of cemented carbide was not a core factor.As a result,the wear resistance of the cemented carbide generally showed a decreasing trend with decrease of the grain size,except for a slight increase in the ultrafine-grained cemented carbide.Single-pass scratching of the cemented carbides under various loads indicated the same failure mechanism as that in the sliding wear tests.Furthermore,the reasons for severe surface oxidation of the coarse-grained cemented carbides were disclosed. | Haibin Wang Mark Gee Qingfan Qiu Hannah Zhang Xuemei Liu Hongbo Nie Xiaoyan Song Zuoren Nie | 2019 | Journal of Materials Science & Technology2019，35，11： | 6 |
| 6 | From the Early Paleozoic Platforms of Baltica and Laurentia to the Caledonide Orogen of Scandinavia and Greenland显示文摘 | David G. Gee Haakon Fossen Niels Henriksen Anthony K. Higgins | 2008 | Episodes2008，31，1： | 5 |
| 7 | 人乳腺癌肿瘤模型:HER2/neu靶向治疗的敏感性及药物剂量的分子成像显示文摘目的利用近红外线光学图像评估原位移植人乳腺癌鼠模型的分子靶向治疗的敏感性及剂量。材料及方法本研究得到动物保护及使用委员会的批准。 | M.S. Gee R. Upadhyay H. Bergquist H. Alencar F. Reynolds M. Maricevich 郑少燕 | 2008 | 国际医学放射学杂志2008，31，A06： | 5 |
| 8 | Polo-like kinase 1,a new therapeutic target in hepatocellular carcinoma显示文摘AIM:To investigate the role of polo-like kinase 1 (PLK1) as a therapeutic target for hepatocellular carcinoma (HCC).METHODS:PLK1 gene expression was evaluated in HCC tissue and HCC cell lines.Gene knockdown with short-interfering RNA (siRNA) was used to study PLK1 gene and protein expression using real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blotting,and cell proliferation using 3-(4,5-dim ethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2(4-sulf ophenyl)-2H-tetrazolium (MTS) and bromodeoxyuridine (BrdU) assays.Apoptosis was evaluated using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay,and caspase-inhibition assay.Huh-7 cells were transplanted into nude mice and co-cultured with PLK1 siRNA or control siRNA,and tumor progression was compared with controls.RESULTS:RT-PCR showed that PLK1 was overexpressed 12-fold in tumor samples compared with controls,and also was overexpressed in Huh-7 cells.siRNA against PLK1 showed a reduction in PLK1 gene and protein expression of up to 96% in Huh-7 cells,and a reduction in cell proliferation by 68% and 92% in MTS and BrdU cell proliferation assays,respectively.There was a 3-fold increase in apoptosis events,and TUNEL staining and caspase-3 assays suggested that this was caspase-independent.The pan-caspase inhibitor Z-VAD-FMK was unable to rescue the apoptotic cells.Immnofluorescence co-localized endonuclease-G to fragmented chromosomes,implicating it in apoptosis.Huh-7 cells transplanted subcutaneously into nude mice showed tumor regression in siPLK1-treated mice,but not in controls.CONCLUSION:Knockdown of PLK1 overexpression in HCC was shown to be a potential therapeutic target,leading to apoptosis through the endonuclease-G pathway. | Wei Chuen Mok Shanthi Wasser Theresa Tan Seng Gee Lim | 2012 | World Journal of Gastroenterology2012，18，27： | 4 |
| 9 | Magnetic resonance elastography for the detection and staging of liver fibrosis in chronic hepatitis B显示文摘 | Sudhakar Kundapur Venkatesh Gang Wang Seng Gee Lim Aileen Wee | 2014 | European Radiology2014，，1： | 4 |
| 10 | RF characteristics of wireless capsule endoscopy in human body显示文摘The wireless capsule endoscope,as a small electronic device,has conquered some limitations of traditional wired diagnosing tools,such as the uncomfortableness of the cables for the patient and the inability to examine the very convoluted small intestine section.However,this technique is still encountering a lot of practical challenges and is looking for feasible improvements.This work investigates the RF performance of the wireless capsule endoscope system by studying the electromagnetic(EM) wave propagation within the human body.A wireless capsule endoscopy transmission channel model is constructed to serve the purpose of investigating signal attenuations according to the relative position between the transmitter and the receiver.Within 300-500 MHz,the S_(21) results are regular and do not display any sudden changes,which allows a suitable expression to be derived for S_(21) in terms of frequency and offset.The results provide useful information for capsule localization. | 王璟琛 王炤 Mark Leach LEE Sanghyuk Eng Gee Lim HUANG Yi | 2016 | Journal of Central South University2016，23，5： | 4 |
| 11 | Long-term Therapy With Adefovir Dipivoxil for HBeAg-Negative Chronic Hepatitis B for up to 5 Years显示文摘 | Stephanos J. Hadziyannis Nicolaos C. Tassopoulos E. Jenny Heathcote Ting–Tsung Chang George Kitis Mario Rizzetto Patrick Marcellin Seng Gee Lim Zachary Goodman Jia Ma Carol L. Brosgart Katyna Borroto–Esoda Sarah Arterburn Steven L. Chuck | 2006 | Gastroenterology2006，，6： | 3 |
| 12 | Chronic hepatitis B: whom to treat and for how long? Propositions, challenges, and future directions显示文摘 | Sang Hoon Ahn Henry L. Y. Chan Pei-Jer Chen Jun Cheng Mahesh K. Goenka Jinlin Hou Seng Gee Lim Masao Omata Teerha Piratvisuth Qing Xie Hyung Joon Yim Man-Fung Yuen | 2010 | Hepatology International2010，，1： | 3 |
| 13 | Detecting active inflammation and fibrosis in pediatric Crohn’s disease: prospective evaluation of MR-E and CT-E显示文摘 | Keith B. Quencer Katherine Nimkin Mari Mino-Kenudson Michael S. Gee | 2013 | Abdominal Imaging2013，，： | 3 |
| 14 | Abdominal ultrasonography of the pediatric gastrointestinal tract显示文摘Ultrasound is an invaluable imaging modality in the evaluation of pediatric gastrointestinal pathology; it can provide real-time evaluation of the bowel without the need for sedation or intravenous contrast. Recent improvements in ultrasound technique can be utilized to improve detection of bowel pathology in children: Higher resolution probes, color Doppler, harmonic and panoramic imaging are excellent tools in this setting. Graded compression and cine clips provide dynamic information and oral and intravenous contrast agents aid in detection of bowel wall pathology. Ultrasound of the bowel in children is typically a targeted exam; common indications include evaluation for appendicitis, pyloric stenosis and intussusception. Bowel abnormalities that are detected prenatally can be evaluated after birth with ultrasound. Likewise, acquired conditions such as bowel hematoma, bowel infections and hernias can be detected with ultrasound. Rare bowel neoplasms, vascular disorders and foreign bodies may first be detected with sonography, as well. At some centers, comprehensive exams of the gastrointestinal tract are performed on children with inflammatory bowel disease and celiac disease to evaluate for disease activity or to confirm the diagnosis. The goal of this article is to review up-to-date imaging techniques, normal sonographic anatomy, and characteristic sonographic features of common and uncommon disorders affecting the gastrointestinal tract in children. | Heather I Gale Michael S Gee Sjirk J Westra Katherine Nimkin | 2016 | World Journal of Radiology2016，8，7： | 3 |
| 15 | Atypical onset of bicalutamide-induced liver injury显示文摘Anti-androgen therapy is the leading treatment for advanced prostate cancer and is commonly used for neoadjuvant or adjuvant treatment. Bicalutamide is a non-steroidal anti-androgen, used during the initiation of androgen deprivation therapy along with a luteinizing hormone-releasing hormone agonist to reduce the symptoms of tumor-related flares in patients with advanced prostate cancer. As side effects, bicalutamide can cause fatigue, gynecomastia, and decreased libido through competitive androgen receptor blockade. Additionally, although not as common, drug-induced liver injury has also been reported. Herein, we report a case of hepatotoxicity secondary to bicalutamide use. Typically, bicalutamideinduced hepatotoxicity develops after a few days; however, in this case, hepatic injury occurred 5 mo after treatment initiation. Based on this rare case of delayed liver injury, we recommend careful monitoring of liver function throughout bicalutamide treatment for prostate cancer. | Gee Young Yun Seok Hyun Kim Seok Won Kim Jong Seok Joo Ju Seok Kim Eaum Seok Lee Byung Seok Lee Sun Hyoung Kang Hee Seok Moon Jae Kyu Sung Heon Young Lee Kyung Hee Kim | 2016 | World Journal of Gastroenterology2016，22，15： | 3 |
| 16 | Risk factors for metachronous gastric carcinoma development after endoscopic resection of gastric dysplasia: Retrospective, single-center study显示文摘AIM To determine the gastric adenocarcinoma(GAC)occurrence rate and related factors,we evaluated the follow-up results of patients confirmed to have gastric dysplasia after endoscopic resection(ER).METHODS We retrospectively analyzed the medical records,endoscopic examination records,endoscopic procedure records,and histological records of 667 cases from641 patients who were followed-up for at least 12mo,from among 1273 patients who were conformed to have gastric dysplasia after Endoscopic mucosal resection(EMR)or endoscopic submucosal dissection (ESD)of gastric mucosal lesions between January 2007and August 2013 at the Chungnam National University Hospital.RESULTS The mean follow-up period was 33.8 mo,and the median follow-up period was 29 mo(range:12-87).During the follow-up period,the occurrence of metachronous GAC was 4.0%(27/667).The mean and median interval periods between the occurrence of metachronous GAC and endoscopic treatment of gastric dysplasia were 36.3 and 34 mo,respectively(range:16-71).The factors related to metachronous GAC occurrence after ER for gastric dysplasia were male sex(5.3%vs 1.0%),open-type atrophic gastritis(9.5%vs3.4%),intestinal metaplasia(6.8%vs 2.4%),and highgrade dysplasia(HGD;8.4%vs 3.2%).Among them,male sex[OR:5.05(1.18-21.68),P=0.029],intestinal metaplasia[OR:2.78(1.24-6.23),P=0.013],and HGD[OR:2.70(1.16-6.26),P=0.021]were independent related factors in multivariate analysis.Furthermore,24of 27 GAC cases(88.9%)occurred at sites other than the previous resection sites,and 3(11.1%)occurred at the same site as the previous resection site.CONCLUSION Male sex,intestinal metaplasia,and HGD were significantly related to the occurrence of metachronous GAC after ER of gastric dysplasia,and most GACs occurred at sites other than the previous resection sites. | Hee Seok Moon Gee Young Yun Ju Seok Kim Hyuk Soo Eun Sun Hyung Kang Jae Kyu Sung Hyun Yong Jeong Kyu-Sang Song | 2017 | World Journal of Gastroenterology2017，23，24： | 3 |
| 17 | Adipose Tissue: The New Endocrine Organ? A Review Article显示文摘 | Susan E. Wozniak Laura L. Gee Mitchell S. Wachtel Eldo E. Frezza | 2009 | Digestive Diseases and Sciences2009，，9： | 3 |
| 18 | Symptom Management for Irritable Bowel Syndrome: A Pilot Randomized Controlled Trial of Acupuncture/Moxibustion显示文摘 | Joyce K. Anastasi Donald J. McMahon Gee H. Kim | 2009 | Gastroenterology Nursing2009，，4： | 3 |
| 19 | NOTES Rectosigmoid Resection Using Transanal Endoscopic Microsurgery (TEM) with Transgastric Endoscopic Assistance: A Pilot Study in Swine显示文摘 | Patricia Sylla Field F. Willingham Dae K. Sohn Denise Gee William R. Brugge David W. Rattner | 2008 | Journal of Gastrointestinal Surgery2008，，10： | 3 |
| 20 | 中国露天生物质燃烧的温室气体排放及秸秆禁燃政策的协同减排效应显示文摘露天生物质燃烧是温室气体(CO_(2)、CH_(4)、N_(2)O)的重要排放源之一,这些排放可影响本地、区域和全球的大气化学和气候变化,并带来一系列生态环境问题,进而对人类及其他生物的生存环境产生影响.本研究采用卫星火点排放清单(FINN),对2010-2019年中国露天生物质燃烧温室气体排放进行研究,并分析秸秆禁燃政策对温室气体排放的影响,对后续秸秆禁燃政策的制定具有重要参考价值.结果表明,我国2010-2019年平均年温室气体排放量为1.53×10^(8) t(将CH_(4)和N_(2)O的温室效应换算为同等效应的CO_(2)当量单位),总体呈波动性下降趋势.温室气体排放量最大的植被类型是森林(7.43×10^(7) t)和农作物(3.19×10^(7) t),分别占总排放量的48.7%和20.9%.从月分布来看,露天生物质燃烧排放的温室气体集中在2-6月,3月是高峰值.从空间分布上看,排放主要集中在华南、西南和华东地区,占总排放量的72.9%.从2013年以来,秸秆禁燃政策的加强在一定程度上降低了露天农作物秸秆燃烧排放的温室气体和PM_(2.5).从变化量看,28个省份实现了秸秆燃烧排放的温室气体与PM_(2.5)同步下降. | 朱永慧 黄凌 李瑜敏 段炼 王杨君 Maggie Chel Gee Ooi Andy Chan 李莉 | 2022 | 环境科学学报2022，42，10： | 3 |
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