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| 1 | Pea3 expression promotes the invasive and metastatic potential of colorectal carcinoma显示文摘AIM:To investigate the function of Pea3 in colorectal carcinoma(CRC)invasion and metastatic potential.METHODS:The expression of Pea3 during clinical progression of human CRC was investigated using Oncomine Research Edition.To assay Pea3 expression in established CRC cell lines,we performed western blotting of cell lysates.We employed sh RNA-mediated knockdown of Pea3 in HCT116(HCT)and LS174T CRC cells which was confirmed by real-time quantitative PCR(q PCR)and western blotting.Transwell invasion assays,MTS proliferation assays,anoikis assays,and fluorometric matrix metalloprotease(MMP)assays wereperformed to determine the effects of Pea3 knockdown on invasion,proliferation,anoikis and MMP activity in CRC cells in vitro.Alterations in epithelial-mesenchymal transition(EMT)and matrix metalloprotease(MMP)m RNA levels were determined by q PCR.CRC cells were injected into the flanks of nude mice to generate xenografts and tumor growth monitored with serial calliper measurements.To assay metastatic potential,CRC cells were injected into the spleen of nude mice,and histological analysis performed on the livers 21 d later.RESULTS:We demonstrated that reduction of Pea3expression in CRC cells significantly impaired their invasive capacity(HCT.sh Pea3,0.28±0.04 fold,P<0.01;LS.sh Pea3,0.15±0.04 fold;SW.sh Pea3,0.23±0.03,P<0.01),reduced anoikis resistance(HCT.sh Pea3 75.4%±1.9%viable cells vs HCT.sh Ctrl 88.6%±0.6%viable cells,P<0.01;LS.sh Pea3 71.7%±0.5%viable cells vs LS.Ctrl 89.6%±0.3%viable cells,P<0.005,but had no effect on proliferation(HCT.sh Ctrl AUC 5098±123 vs HCT.sh Pea3 5689±151,P<0.05;LS.sh Ctrl AUC 5600±324.1 vs LS.sh Pea3 6423±400,P<0.05).In vivo,HCT.sh Pea3 and HCT.sh Ctrl tumour xenografts grew at a similar rate(HCT.sh Pea3 2.64±0.82 fold vs HCT.sh Ctrl 2.88±0.80 fold,P>0.05).In keeping with a pro-metastatic function for Pea3 in CRC,several EMT markers and MMPs were downregulated in sh Pea3-expressing cells,suggesting that Pea3 may exert its effects through these processes.A reduction in overall MMP activity was observed in HCT.sh Pea3 cells compared to their control counterparts(HCT.sh Pea30.61±0.04 fold,P<0.005).This translated in vivo to the complete absence of metastases in the livers of mice that were grafted with CRC cells lacking Pea3.Conversely,CRC cells expressing Pea3 formed liver metastases in all mice.CONCLUSION:Our study implicates Pea3 as a mediator of metastases,and provides a biological rationale for the adverse prognosis associated with elevated Pea3 expression in human CRC. | Aruz Mesci Samira Taeb Xiaoyong Huang Rishi Jairath Darshan Sivaloganathan Stanley K Liu | 2014 | World Journal of Gastroenterology2014，20，46： | 7 |
| 2 | Flux blended synthesis of novel Y2O3:Eu^3+ sensing arrays for highly sensitive dual mode detection of LFPs on versatile surfaces显示文摘In the present communication,various fluxes blended Y_2 O_3:Eu^(3+)(5 mol%) nanopowders(NPs) were successfully fabricated by solution combustion method.PXRD pattern confirms body-centered cubic structure of the prepared samples.Energy band gap(Eg) of the fabricated products was estimated and is found to be in the range of 3.13-3.32 eV.Photoluminescence(PL) emission spectra exhibit sharp and intense peaks at ~579,592,614,657,704 nm corresponding to ~5 D_0→~7 F_J(J = 0,1,2,3 and 4) transitions of Eu^(3+) ions.Significance of fluxes for enhancing the PL emissions was extensively studied.Photometric studies of the prepared samples are located in pure red region.Optimized NPs were explored as a novel sensing agent for visualization of latent fingerprints(LFPs) on various surfaces including porous,semiporous and non-porous surfaces followed by powder dusting technique.Various experiments including aging,temperature,scratching and aquatic fresh water treatment tests were performed to evaluate applicability of the fabricated NPs.Visualized LFPs exhibit well defined ridge details including most authenticated sweat pores are also revealed with high sensitivity,selectivity,little background hindrance and less toxicity.Aforementioned results evidence that the method and fabricated NPs can be considered to be simple,rapid and economical and provide novel sensing platform for LFPs visualization in prospective forensic applications. | K.N. Venkatachalaiah H. Nagabhushana R.B. Basavaj G.R Darshan B. Daruka Prasad S.C. Sharma | 2018 | Journal of Rare Earths2018，36，9： | 6 |
| 3 | Steroid resistance in leukemia显示文摘There are several types of leukemia which are characterized by the abnormal growth of cells from the myeloid or lymphoid lineage. Because of their lympholytic actions, glucocorticoids(GCs) are included in many therapeutic regimens for the treatment of various forms of leukemia. Although a significant number of acute lymphoblastic leukemia patients respond well to GC treatment during initial phases; prolonged treatments sometimes results in steroid-resistance. The exact mechanism of this resistance has yet not been completely elucidated, but a correlation between functional GC receptor expression levels and steroidresistance in patients has been found. In recent years, several other mechanisms of action have been reported that could play an important role in the development of such drug resistances in leukemia. Therefore, a better understanding of how leukemic patients develop drug resistance should result in drugs designed appropriately to treat these patients. | Darshan S Shah Raj Kumar | 2013 | World Journal of Experimental Medicine2013，3，2： | 4 |
| 4 | Evaluating the accuracy of American Society for Gastrointestinal Endoscopy guidelines in patients with acute gallstone pancreatitis with choledocholithiasis显示文摘BACKGROUND Acute gallstone pancreatitis(AGP) is the most common cause of acute pancreatitis(AP) in the United States. Patients with AGP may also present with choledocholithiasis. In 2010, the American Society for Gastrointestinal Endoscopy(ASGE) suggested a management algorithm based on probability for choledocholithiasis, recommending additional imaging for patients at intermediate risk and endoscopic retrograde cholangiopancreatography(ERCP) for patients at high risk of choledocholithiasis. In 2019, the ASGE guidelines were updated using more specific criteria to categorize individuals at high risk for choledocholithiasis. Neither ASGE guideline has been studied in AGP to determine the probability of having choledocholithiasis.AIM To determine compliance with ASGE guidelines, assess outcomes, and compare 2019 vs 2010 ASGE criteria for suspected choledocholithiasis in AGP.METHODS We conducted a retrospective cohort study of 882 patients admitted with AP to a single tertiary care center from 2008-2018. AP was diagnosed using revised Atlanta criteria and AGP was defined as the presence of gallstones on imaging or with cholestatic pattern of liver injury in the absence of another cause. Patients with chronic pancreatitis and pancreatic malignancy were excluded as were those who went directly to cholecystectomy prior to assessment for choledocholithiasis. Patients were assigned low, intermediate or high risk based on ASGE guidelines. Our primary outcomes of interest were the proportion of patients in the intermediate risk group undergoing magnetic resonance cholangiopancreatography(MRCP) first and the proportion of patients in the high risk group undergoing ERCP directly without preceding imaging. Secondary outcomes of interest included outcome differences based on if guidelines were not adhered to. We then evaluated the diagnostic accuracy of 2019 in comparison to the 2010 ASGE criteria for patients with suspected choledocholithiasis. We performed the t test or Wilcoxon rank sum test, as appropriate, to analyze if there were outcome differences based on if guidelines were not adhered to. Kappa coefficients were calculated to measure the degree of agreement between pairs of variables.RESULTS In this cohort, we identified 235 patients with AGP of which 79 patients were excluded as they went directly to surgery for cholecystectomy without prior MRCP or ERCP. Of the remaining 156 patients, 79 patients were categorized as intermediate risk and 77 patients were high risk for choledocholithiasis according to the 2010 ASGE guidelines. Among 79 intermediate risk patients, 54(68%) underwent MRCP first whereas 25 patients(32%) went directly to ERCP. For the 54 patients with intermediate risk who had MRCP first, 36 patients had no choledocholithiasis while 18 patients had evidence of choledocholithiasis prompting ERCP. Of these patients, ERCP confirmed stone disease in 11 patients. Of the 25 intermediate risk patients who directly underwent ERCP, 18 patients had stone disease. One patient with a normal ERCP developed post ERCP pancreatitis. Patients undergoing MRCP in this group had a significantly longer length of stay(5.0 vs 4.0 d, P = 0.02). In the high risk group, 64 patients(83%) had ERCP without preceding imaging, of which, 53 patients had findings consistent with choledocholithiasis, of which 13 patients(17%) underwent MRCP before ERCP, all of which showed evidence of stone disease. Furthermore, all of these patients ultimately had an ERCP, of which 8 patients had evidence of stones and 5 had normal examination.RESULTS Our cohort also demonstrated that 58% of all 156 patients with AGP had confirmed choledocholithiasis(79% of the high risk group and 37% of the intermediate group when risk was assigned based on the 2010 ASGE guidelines). When the updated 2019 ASGE guidelines were applied instead of the original 2010 guidelines, there was moderate agreement between the 2010 and 2019 guidelines(kappa = 0.46, 95%CI: 0.34-0.58). Forty-two of 77 patients were still deemed to be high risk and 35 patients were downgraded to intermediate risk. Thirty-five patients who were originally assigned high risk were reclassified as intermediate risk. For these 35 patients, 26 patients had ERCP findings consistent with choledocholithiasis and 9 patients had a normal examination. Based on the 2019 criteria, 9/35 patients who were downgraded to intermediate risk had an unnecessary ERCP with normal findings(without a preceding MRCP).CONCLUSION Two-thirds in intermediate risk and 83% in high risk group followed ASGE guidelines for choledocholithiasis. One intermediate-group patient with normal ERCP had post-ERCP AP, highlighting the risk of unnecessary procedures. | Supisara Tintara Ishani Shah William Yakah Awais Ahmed Cristina S Sorrento Cinthana Kandasamy Steven D Freedman Darshan J Kothari Sunil G Sheth | 2022 | World Journal of Gastroenterology2022，28，16： | 3 |
| 5 | Electrocardiographic QT Interval and Mortality: A Meta-analysis显示文摘 | Yiyi Zhang Wendy S. Post Elena Blasco-Colmenares Darshan Dalal Gordon F. Tomaselli Eliseo Guallar | 2011 | Epidemiology2011，，5： | 2 |
| 6 | 胰外并发症尤其是血液透析可预测ICU急性胰腺炎患者的病死率和住院时间显示文摘背景和目的:入住ICU的急性胰腺炎患者因疾病严重和住院时间延长,胰外并发症风险增高。我们旨在评估ICU急性胰腺炎患者胰外并发症发生率及其对住院时间和病死率的影响。方法:本研究为回顾性队列研究,研究对象来自一家三级转诊中心的287例ICU急性胰腺炎患者,其中163例满足纳入标准入组研究。计算胰外并发症发生率,并通过单因素和多因素分析评估胰外并发症对住院时间和病死率的影响。结果:163例患者共出现158例次胰外并发症,平均每例患者出现0.97种胰外并发症。95例患者出现至少1种胰外并发症,另外68例未出现胰外并发症。在排除胰腺局部并发症患者后,胰外并发症患者住院时间较无并胰外发症患者显著延长(14.7天vs.8.8天,P<0.01)。非感染性胰外并发症患者病死率显著增高(24.0%vs.16.2%,P=0.04)。多因素分析结果显示,需要透析是住院时间(IRR=1.73,95%CI:1.2632.378,P<0.01)和病死率(IRR=1.50,95%CI:1.6236.843,P<0.01)的独立预测因素。同时,冠脉事件也是病死率的一个预测因素(P=0.05)。而其他胰外并发症的预测价值未获证实。结论:ICU急性胰腺炎患者常会发生胰外并发症,从而影响住院时间。非感染性胰外并发症还会增加病死率。在消除了胰腺局部并发症的干扰后,需要透析治疗被证实为ICU急性胰腺炎患者住院时间延长和病死率增高的独立预测因素。 | Darshan Kothari Maarten R.Struyvenberg Michael C.Perillo Ghideon Ezaz Steven D.Freedman Sunil G.Sheth | 2018 | Gastroenterology Report2018，6，3： | 2 |
| 7 | Toxic megacolon associated Clostridium difficile colitis显示文摘Toxic megacolon is a severe complication of Clostridium difficile (C.difficile) colitis.As the prevalence of C. difficile colitis increases and treatments become more refractory, clinicians will encounter more patients with C. difficile associated toxic megacolon in the future. Here, we review a case of toxic megacolon secondary to C. difficile colitis and review the current literature on diagnosis and management. We identify both clinical and radiologic criteria for diagnosis and discuss both medical and surgical options for management. Ultimately, we recommend using the Jalen criteria in conjunction with daily abdominal radiographs to help establish the diagnosis of toxic megacolon and to make appropriate treatment recommendations. Aggressive medical management using supportive measures and antibiotics should remain the mainstay of treatment. Surgical intervention should be considered if the patient does not clinically improve within 2-3 d of initial treatment. | Leena Sayedy Darshan Kothari Robert J Richards | 2010 | World Journal of Gastrointestinal Endoscopy2010，2，8： | 2 |
| 8 | Liver-gut axis in the regulation of iron homeostasis显示文摘人的身体为它的正常工作每天要求铁的大约 1-2 mg,并且饮食的铁是为这必需品的唯一的来源金属。因为人不为铁的活跃排泄拥有机制,在身体的铁的数量被越过近似小肠并且,因而吸收的数量决定肠的铁吸收是一个高度调整的过程。在最近的年里,肝从另外的纸巾作为铁吸收和铁版本的一个中央管理者出现了。它由肽荷尔蒙把对小肠上皮和另外的房间起作用限制铁交货到血浆的 hepcidin 称为的 secreting 完成这。Hepcidin 本身在身体铁店,红血球生成的率,发炎和组织缺氧包括变化响应各种各样的全身的刺激被调整,以许多年著名调制的一样的刺激熨吸收。这评论将在肝和 hepcidin 在身体铁吸收的规定起的作用上总结最近的调查结果。 | Deepak Darshan Gregory J Anderson | 2007 | World Journal of Gastroenterology2007，13，35： | 2 |
| 9 | Determination of lercanidipine in human plasma by an improved UPLC–MS/MS method for a bioequivalence study显示文摘An improved and reliable ultra-performance liquid chromatography/tandem mass spectrometry(UPLC–MS/MS) method has been developed and validated for the determination of lercanidipine in human plasma. Plasma samples with lercanidipine-d3 as an internal standard(IS) were prepared by solid phase extraction on Phenomenex Strata-X cartridges using 100 μL of human plasma. Chromatographic analysis was performed on UPLC BEH C_(18)(50 mm*2.1 mm, 1.7μm) column under isocratic conditions. Linear calibration curves were obtained over a wide dynamic concentration range of 0.010–20.0 ng/mL. Matrix effect was assessed by post-column infusion, post-extraction spiking and standard-line slope methods.The mean extraction recovery was >94% for the analyte and IS. Inter-batch and intra-batch precision(%CV) across five quality controls was <5.8%. Bioequivalence study was performed with 36 healthy subjects after oral administration of 10 mg of lercanidipine and the assay reproducibility was evaluated by reanalysis of 133 incurred samples. | Darshan V.Chaudhary Daxesh P.Patel Priyanka A.Shah Jaivik V.Shah Mallika Sanyal Pranav S.Shrivastav | 2016 | Journal of Pharmaceutical Analysis2016，6，2： | 2 |
| 10 | Arrhythmogenic Right Ventricular Dysplasia: A United States Experience显示文摘 | Darshan Dalal Khurram Nasir Chandra Bomma Kalpana Prakasa Harikrishna Tandri Jonathan Piccini Ariel Roguin Crystal Tichnell Cynthia James Stuart D. Russell Daniel P. Judge Theodore Abraham Philip J. Spevak David A. Bluemke Hugh Calkins | 2005 | Circulation2005，，25： | 1 |
| 11 | Development of introgression lines of an Indica-type rice variety, IR64, for unique agronomic traits and detection of the responsible chromosomal regions显示文摘 | Daisuke Fujita Rizza E. Santos Leodegario A. Ebron Mary J. Telebanco-Yanoria Hiroshi Kato Sohei Kobayashi Yusaku Uga Etsuko Araki Toshiyuki Takai Hiroshi Tsunematsu Tokio Imbe Gurdev S. Khush Darshan S. Brar Yoshimichi Fukuta Nobuya Kobayashi | 2009 | Field Crops Research2009，，2： | 1 |
| 12 | Thermal decomposition of zinc-iron citrate precursor显示文摘 | GAJBHIYE N S BHATYACHARYA U DARSHANE V S | 1995 | Thermochimica Acta1995，264，： | 1 |
| 13 | Regulation of systemic iron homeostasis: how the body responds to changes in iron demand显示文摘 | Gregory J. Anderson Deepak Darshan Sarah J. Wilkins David M. Frazer | 2007 | BioMetals2007，，3： | 1 |
| 14 | Electrocardiographic Features of Arrhythmogenic Right Ventricular Dysplasia/Cardiomyopathy According to Disease Severity: A Need to Broaden Diagnostic Criteria显示文摘 | Khurram Nasir Chandra Bomma Harikrishna Tandri Ariel Roguin Darshan Dalal Kalpana Prakasa Crystal Tichnell Cynthia James Phillip Jspevak Frank Marcus Hugh Calkins | 2004 | Circulation2004，，12： | 1 |
| 15 | Subrat Sahu and Piyush Kumar Sinha, Role of Dynamic Capability and Information Technology in Customer Relationship Management: A Study of Indi- an Companies 显示文摘 | Darshan Desai | 2007 | Journal for decision makers2007，，32： | 1 |
| 16 | Tranexamic acid for intracerebral haemorrhage within 2 hours of onset: protocol of a phase Ⅱ randomised placebo-controlled double-blind multicentre trial显示文摘Rationale Haematoma growth is common early after intracerebral haemorrhage(ICH),and is a key determinant of outcome.Tranexamic acid,a widely available antifibrinolytic agent with an excellent safety profile,may reduce haematoma growth.Methods and design Stopping intracerebral haemorrhage with tranexamic acid for hyperacute onset presentation including mobile stroke units(STOP-MSU)is a phase Ⅱ double-blind,randomised,placebo-controlled,multicentre,international investigator-led clinical trial,conducted within the estimand statistical framework.Hypothesis In patients with spontaneous ICH,treatment with tranexamic acid within 2 hours of onset will reduce haematoma expansion compared with placebo.Sample size estimates A sample size of 180 patients(90 in each arm)would be required to detect an absolute difference in the primary outcome of 20%(placebo 39%vs treatment 19%)under a two-tailed significance level of 0.05.An adaptive sample size re-estimation based on the outcomes of 144 patients will allow a possible increase to a prespecified maximum of 326 patients.Intervention Participants will receive 1 g intravenous tranexamic acid over 10 min,followed by 1 g intravenous tranexamic acid over 8 hours;or matching placebo.Primary efficacy measure The primary efficacy measure is the proportion of patients with haematoma growth by 24±6 hours,defined as either≥33%relative increase or≥6 mL absolute increase in haematoma volume between baseline and follow-up CT scan.Discussion We describe the rationale and protocol of STOP-MSU,a phase Ⅱ trial of tranexamic acid in patients with ICH within 2 hours from onset,based in participating mobile stroke units and emergency departments. | Nawaf Yassi Henry Zhao Leonid Churilov Bruce C V Campbell Teddy Wu Henry Ma Andrew Cheung Timothy Kleinig Helen Brown Philip Choi Jiann-Shing Jeng Annemarei Ranta Hao-Kuang Wang Geoffrey C Cloud Rohan Grimley Darshan Shah Neil Spratt Der-Yang Cho Karim Mahawish Lauren Sanders John Worthington Ben Clissold Atte Meretoja Vignan Yogendrakumar Mai Duy Ton Duc Phuc Dang Nguyen Thai My Phuong Huy-Thang Nguyen Chung Y Hsu Gagan Sharma Peter J Mitchell Bernard Yan Mark W Parsons Christopher Levi Geoffrey A Donnan Stephen M Davis | 2022 | Stroke & Vascular Neurology2022，7，2： | 1 |
| 17 | Personalized synthetic lethality induced by targeting RAD52 in leukemias identified by gene mutation and expression profile显示文摘 | Kimberly Cramer-Morales Margaret Nieborowska-Skorska Kara Scheibner Michelle Padget David A. Irvine Tomasz Sliwinski Kimberly Haas Jaewoong Lee Huimin Geng Darshan Roy Artur Slupianek Feyruz V. Rassool Mariusz A. Wasik Wayne Childers Mhairi Copland Markus | 2013 | Blood2013，，7： | 1 |
| 18 | An alliance based peering scheme for P2P live media streaming 显示文摘 | Darshan Purandare Ratan K Guha | 2007 | IEEE Transactions on Multimedia2007，9，8： | 1 |
| 19 | Nanostructured nickel ferrite: A liquid petroleum gas sensor 显示文摘 | Darshane S L Suryanvanshi S S Mulla I S | 2009 | Ceramics Inter- national2009，35，5： | 1 |
| 20 | Initial experience in the use of integrated electroanatomic mapping with three-dimensional MR/CT images to guide catheter ablation of atrial fibrillation显示文摘 | Jun D Timm D Darshan D | 2006 | J Cardovasc Eletrophysiol2006，17，： | 1 |
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