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| 1 | Hijacking antibody-induced CTLA-4 lysosomal degradation for safer and more effective cancer immunotherapy显示文摘It remains unclear why the clinically used anti-CTLA-4 antibodies, popularly called checkpoint inhibitors, have severe immunotherapy-related adverse effects (irAEs) and yet suboptimal cancer immunotherapeutic effects (CITE). Here we report that while irAE-prone Ipilimumab and TremeIgG1 rapidly direct cell surface CTLA-4 for lysosomal degradation, the non-irAE-prone antibodies we generated, HL12 or HL32, dissociate from CTLA-4 after endocytosis and allow CTLA-4 recycling to cell surface by the LRBA-dependent mechanism. Disrupting CTLA-4 recycling results in robust CTLA-4 downregulation by all anti-CTLA-4 antibodies and confers toxicity to a non-irAE-prone anti-CTLA-4 mAb. Conversely, increasing the pH sensitivity of TremeIgG1 by introducing designed tyrosine-to-histidine mutations prevents antibody-triggered lysosomal CTLA-4 downregulation and dramatically attenuates irAE. Surprisingly, by avoiding CTLA-4 downregulation and due to their increased bioavailability, pH-sensitive anti-CTLA-4 antibodies are more effective in intratumor regulatory T-cell depletion and rejection of large established tumors. Our data establish a new paradigm for cancer research that allows for abrogating irAE while increasing CITE of anti-CTLA-4 antibodies. | Yan Zhang Xuexiang Du Mingyue Liu Fei Tang Peng Zhang Chunxia Ai James K. Fields Eric J. Sundberg Olga S. Latinovic Martin Devenport Pan Zheng Yang Liu | 2019 | Cell Research2019，29，8： | 6 |
| 2 | Effects of Shrub on Runoff and Soil Loss at Loess Slopes Under Simulated Rainfall显示文摘Improved understanding of the effect of shrub cover on soil erosion process will provide valuable information for soil and water conservation programs.Laboratory rainfall simulations were conducted to determine the effects of shrubs on runoff and soil erosion and to ascertain the relationship between the rate of soil loss and the runoff hydrodynamic characteristics.In these simulations a 20° slope was subjected to rainfall intensities of 45,87,and 127 mm/h.The average runoff rates ranged from 0.51 to 1.26 mm/min for bare soil plots and 0.15 to 0.96 mm/min for shrub plots.Average soil loss rates varied from 44.19 to 114.61 g/(min·m^2) for bare soil plots and from 5.61 to 84.58 g/(min·m^2) for shrub plots.There was a positive correlation between runoff and soil loss for the bare soil plots,and soil loss increased with increased runoff for shrub plots only when rainfall intensity is 127 mm/h.Runoff and soil erosion processes were strongly influenced by soil surface conditions because of the formation of erosion pits and rills.The unit stream power was the optimal hydrodynamic parameter to characterize the soil erosion mechanisms.The soil loss rate increased linearly with the unit stream power on both shrub and bare soil plots.Critical unit stream power values were 0.004 m/s for bare soil plots and 0.017 m/s for shrub plots. | XIAO Peiqing YAO Wenyi SHEN Zhenzhou YANG Chunxia LYU Xizhi JIAO Peng | 2017 | Chinese Geographical Science2017，27，4： | 5 |
| 3 | Comparison of nonhuman primates identified the suitable model for COVID-19显示文摘Identification of a suitable nonhuman primate(NHP)model of COVID-19 remains challenging.Here,we characterized severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in three NHP species:Old World monkeys Macaca mulatta(M.mulatta)and Macaca fascicularis(M.fascicularis)and New World monkey Callithrix jacchus(C.jacchus).Infected M.mulatta and M.fascicularis showed abnormal chest radiographs,an increased body temperature and a decreased body weight.Viral genomes were detected in swab and blood samples from all animals.Viral load was detected in the pulmonary tissues of M.mulatta and M.fascicularis but not C.jacchus.Furthermore,among the three animal species,M.mulatta showed the strongest response to SARS-CoV-2,including increased inflammatory cytokine expression and pathological changes in the pulmonary tissues.Collectively,these data revealed the different susceptibilities of Old World and New World monkeys to SARS-CoV-2 and identified M.mulatta as the most suitable for modeling COVID-19. | Shuaiyao Lu Yuan Zhao Wenhai Yu Yun Yang Jiahong Gao Junbin Wang Dexuan Kuang Mengli Yang Jing Yang Chunxia Ma Jingwen Xu Xingli Qian Haiyan Li Siwen Zhao Jingmei Li Haixuan Wang Haiting Long Jingxian Zhou Fangyu Luo Kaiyun Ding Daoju Wu Yong Zhang Yinliang Dong Yuqin Liu Yinqiu Zheng Xiaochen Lin Li Jiao Huanying Zheng Qing Dai Qiangming Sun Yunzhang Hu Changwen Ke Hongqi Liu Xiaozhong Peng | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 4 | A quality evaluation strategy for Rhizoma coptidis from a variety of different sources using chromatographic fingerprinting combined with biological fingerprinting显示文摘Most of herbal medicines of different sources are used for the same treatment goal in clinic despite their tremendous differences of the quality.Because the clinical efficacy is closely related to the quality of medicines,the implementation of an effective quality control(QC)process is vital.Chemical methods based on content determination and chromatographic fingerprints(CFs)have been recommended as powerful tools.In spite of advances in QC,it is still difficult to determine that which of the chemical compositions can best reflect the correlation with the desired biological activity.Herein,we have focused on the biological activities of multi-source herbal medicines to establish an analytical method.By looking for correlations between the biological activities and their chemical compositions,the method can identify reasonable chemical indicators or chemical methods with biological activity information.In this study,high-performance liquid chromatography(HPLC)and microcalorimetry are combined as a useful method for evaluating the quality of Rhizoma coptidis from different sources.The results of biological fingerprints(BFs)showed that the antibacterial activity sequence of R.coptidis of different sources was Coptis teeta Wall.(CT)>Coptis chinensis Franch.(CC)>Coptis deltoidea C.Y.Cheng et Hsiao(CD).The'spectrum-activity'relationships between CFs and BFs were investigated using the canonical correlation analysis(CCA).Compared with the CCA result of CFs and BFs,the canonical correlation coefficient r1and r2were 0.9974 and 0.9752,respectively.It revealed that there are a close correlation between the spectrum-activity relationships and several of the major anti-bacterial components in the CFs,including jatrorrhizine,coptisine,palmatine,and berberine.Of course,R.coptidis was only a representative of multisource medical plants.The result of the current study will provide an effective reference for evaluating the quality of multisource herbal medicines. | LI JunXian YAN Dan MA LiNa XIONG Yin YAN ChunXia LI BaoCai PENG Cheng XIAO XiaoHe | 2013 | Chinese Science Bulletin2013，58，33： | 5 |
| 5 | Effects of L-3-n-butylphthalide on caspase-3 and nuclear factor kappa-B expression in primary basal forebrain and hippocampal cultures after beta-amyloid peptide 1-42 treatment显示文摘BACKGROUND:L-3-n-butylphthalide(L-NBP) can inhibit phosphorylation of tau protein and reduce the neurotoxicity of beta-amyloid peptide 1-42(Aβ_(1-42)). OBJECTIVE:To observe the neuroprotective effects of L-NBP on caspase-3 and nuclear factor kappa-B(NF-κB) expression in a rat model of Alzheimer's disease. DESIGN,TIME AND SETTING:A cell experiment was performed at the Central Laboratory of Provincial Hospital affiliated to Shandong University between January 2008 and August 2008. MATERIALS:L-NBP(purity>98%) was provided by Shijiazhuang Pharma Group NBP Pharmaceutical Company Limited.Aβ_(1-42),3-[4,5-dimethylthiazolo-2]-2,5 iphenyltetrazolium bromide(MTT),and rabbit anti-Caspase-3 polyclonal antibody were provided by Cell Signaling, USA;goat anti-choactase and rabbit anti-NF-κB antibodies were provided by Santa Cruz,USA. METHODS:Primary cultures were generated from rat basal forebrain and hippocampal neurons at 17 or 19 days of gestation.The cells were assigned into five groups:the control group,the Aβ_(1-42) group(2μmol/ L),the Aβ_(1-42) + 0.1μmol/L L-NBP group,the Aβ_(1-42)+ 1μmol/L L-NBP group,and the Aβ_(1-42)+ 10μmol/L L-NBP group.The neurons were treated with Aβ_(1-42)(2μmol/L) alone or in combination with L-NBP(0.1,1,10μmol/L) for 48 hours.Cells in the control group were incubated in PBS. MAIN OUTCOME MEASURES:Morphologic changes were evaluated using inverted microscopy, viability using the MTT method,and the changes in caspase-3 and NF-κB expression using Western blot. RESULTS:Induction with Aβ_(1-42) for 48 hours caused cell death and soma atrophy,and increased caspase-3 and NF-κB expression(P<0.05).L-NBP blocked these changes in cell morphology, decreased caspase-3 and NF-κB expression(P<0.05),and improved cell viability,especially at the high dose(P<0.05). CONCLUSION:Aβ_(1-42) is toxic to basal forebrain and hippocampal primary neurons;L-NBP protects against this toxicity and inhibits the induction of caspase-3 and NF-κB expression. | Ruixia Wang Yong Zhang Liangliang Jiang Guozhao Ma Qingxi Fu Jialong Li Peng Yan Lunqian Shen Yabo Feng Chunxia Li Zaiying Pang Yuanxiao Cui Chunfu Chen Yifeng Du Zhaokong Liu | 2009 | Neural Regeneration Research2009，4，4： | 3 |
| 6 | Advances in Biodegradation of Aflatoxins显示文摘Aflatoxins are secondary metabolites of fungi such as Aspergillus flavus and Aspergillus parasiticus. They are one of the contaminants most common in food and feed, with high toxicity and carcinogenicity. Aflatoxins usually enter animal body together with feed and then enter human body by food chain, thereby seriously threatening human health. In recent years, the degradation of aflatoxins has become a hot research topic. This study overviewed the characteristics and detoxification ways of aflatoxins, specifically for the advances in biodegradation and degradation products of aflatoxins. | Yi WANG Chunxia ZHAO Yucai LYU Wei CHENG Peng GUO | 2016 | Agricultural Science & Technology2016，17，4： | 2 |
| 7 | AATYK is a Novel Regulator of Oligodendrocyte Differentiation and Myelination显示文摘Oligodendrocytes(OLs) are myelinating glial cells that form myelin sheaths around axons to ensure rapid and focal conduction of action potentials. Here, we found that an axonal outgrowth regulatory molecule, AATYK(apoptosis-associated tyrosine kinase), was up-regulated with OL differentiation and remyelination. We therefore studied its role in OL differentiation. The results showed that AATYK knockdown inhibited OL differentiation and the expression of myelin genes in vitro. Moreover, AATYKdeficiency maintained the proliferation status of OLs but did not affect their survival. Thus, AATYK is essential for the differentiation of OLs. | Chunxia Jiang Wanqing Yang Zhihong Fan Peng Teng Ruyi Mei Junlin Yang Aifen Yang Mengsheng Qiu Xiaofeng Zhao | 2018 | Neuroscience Bulletin2018，34，3： | 2 |
| 8 | Urban Fresh Water Resources Consumption of China显示文摘From the point of view of urban consumption behavior, urban fresh water consumption could be classified as three types, namely, direct, indirect and induced water consumption. A calculation approach of urban fresh water consumption was presented based on the theory of urban basic material consumption and the input-output method, which was utilized to calculate urban fresh water consumption of China, and to analyze its structural change and causes. The results show that the total urban fresh water consumption increased 561.7×109m3, and the proportion to the total national fresh water resources increased by 20 percentage points from 1952 to 2005. The proportion of direct and induced water consumption had been continuously rising, and it increased by 15 and 35 percentage points separately from 1952 to 2005, while the proportion of indirect water consumption decreased by 50 percentage points. Urban indirect water consumption was mainly related to urban grain, beef and mutton consumption, and urban induced water consumption had a close relationship with the amount of carbon emission per capita. Finally, some countermeasures were put forward to realize sustainable utilization of urban fresh water resources in China. | ZHU Peng LU Chunxia ZHANG Lei CHENG Xiaoling | 2009 | Chinese Geographical Science2009，19，3： | 2 |
| 9 | Cross-Modal Complementary Network with Hierarchical Fusion for Multimodal Sentiment Classification显示文摘Multimodal Sentiment Classification(MSC)uses multimodal data,such as images and texts,to identify the users'sentiment polarities from the information posted by users on the Internet.MSC has attracted considerable attention because of its wide applications in social computing and opinion mining.However,improper correlation strategies can cause erroneous fusion as the texts and the images that are unrelated to each other may integrate.Moreover,simply concatenating them modal by modal,even with true correlation,cannot fully capture the features within and between modals.To solve these problems,this paper proposes a Cross-Modal Complementary Network(CMCN)with hierarchical fusion for MSC.The CMCN is designed as a hierarchical structure with three key modules,namely,the feature extraction module to extract features from texts and images,the feature attention module to learn both text and image attention features generated by an image-text correlation generator,and the cross-modal hierarchical fusion module to fuse features within and between modals.Such a CMCN provides a hierarchical fusion framework that can fully integrate different modal features and helps reduce the risk of integrating unrelated modal features.Extensive experimental results on three public datasets show that the proposed approach significantly outperforms the state-of-the-art methods. | Cheng Peng Chunxia Zhang Xiaojun Xue Jiameng Gao Hongjian Liang Zhengdong Niu | 2022 | Tsinghua Science and Technology2022，27，4： | 2 |
| 10 | Immobilized trypsin onto chitosan modified monodisperse microspheres:A different way for improving carrier's surface biocompatibility显示文摘 | Peng Gang Zhao Chunxia Liu Bailing | | 0，，15： | 1 |
| 11 | Analysis of the TCD results in hypertensive cerebral arteriosclerosis显示文摘 | HUANG Chunxia WANG Peng | | 0，，04： | 1 |
| 12 | Self-activated luminescent and mesoporous strontium hydroxyapatite nanorods for drug delivery显示文摘 | Cuimiao Zhang Chunxia Li Shanshan Huang Zhiyao Hou Ziyong Cheng Piaoping Yang Chong Peng Jun Lin | 2010 | Biomaterials2010，，12： | 1 |
| 13 | Preparation of Rice Seedling-raising Substrate with Spent Mushroom Substrate of Pleurotus eryngii显示文摘This study aimed to explore the effect of fermented spent mushroom substrate(SMS) of Pleurotus eryngii as a basic material on rice seedling-raising substrate. The physical and chemical indices of the SMS indicated that the fermented SMS was fully composted and was very suitable for preparing rice seedling-raising substrate. The fermented SMS effectively regulated the bulk density, total porosity, aeration porosity and water-holding porosity of rice seedling-raising substrate. With the increased addition amount of fermented SMS, the bulk density of rice seedling-raising substrate was decreased, but the total porosity, aeration porosity and water-holding porosity were increased. Compared with those in the substrates of 100% soil and 100% SMS,the height, chlorophyll content, 100-shoot dry weight, 100-root dry weight, root activity, nitrogen content, phosphorus content and potassium content of rice seedlings in the substrate composed of spent mushroom substrate of P. eryngii and soil were higher.The quality of rice seedlings in the substrate composed of 20% soil and 80% SMS was best, followed by that in the substrate composed of 30% soil and 70% SMS. | Dan ZHENG Yi WANG Chunxia ZHAO Jinguo CHEN Wei CHENG Peng GUO Zongjun CUI | 2016 | Agricultural Science & Technology2016，17，2： | 1 |
| 14 | The catalytic Baeyer- Villiger oxidation of cyelohexanone to e-caprolactone over stibium- containing hydrotalcite显示文摘 | Chen Chunxia Peng Jinsong Li Bin | 2009 | Catalysis Letters2009，131，34： | 1 |
| 15 | Bilateral papilledema caused by chronic infantile neurological cutaneous and articular syndrome in a child with a novel (p. D305N) mutation in NLRP3 gene: a case report显示文摘The rare disease of chronic infantile neurological cutaneous and articular(CINCA)syndrome,is caused by the over-secretion of interleukin(IL)-1βdue to a gain-of-function NLRP3 gene mutation in the autosomal chromosome which often involves in eyes.In this report,we studied a 9-year-old girl with CINCA.The eyes were also involved and presented bilateral papilledema.Genetic testing revealed that the symptoms were caused by a novel gene mutation site(c.913G>A,p.D305N)in conservative domain exon-3 of NLRP3 which is gain-function gene of CINCA.The patient had the characteristic facial features,frontal fossa and saddle nose,manifested the generalized urticaria-like skin rash at two weeks after birth,periodic fever 6 months after birth,sensorineural deafness at 7 years old,and bilateral papilledema,aseptic meningitis and knee arthropathy at 9 years old.White cell counts,C-reactive protein increased and intracranial pressure raised to 300 mmH2O.The meningeal thickening enhanced by gadolinium in magnetic resonance imaging(MRI).Based on clinical features and genetic test,the girl was diagnosed bilateral papilledema secondary to CINCA and administered prednisone and lowered intracranial pressure medicine to resolve symptoms.With 3-year follow-up,patient had no inflammatory flare-up with visual acuity improvement.The finding of novel genetic mutation site(p.D305N)in NLRP3 gene expanded genotype spectrum associated with CINCA.This case also expanded the cause spectrum of papilledema and it highlighted systemic disease history for patients with bilateral papilledema. | Li Li Huanfen Zhou Wei Shi Xiaotun Ren Xiaohua Tan Lirong Tian Chunxia Peng | 2021 | Annals of Eye Science2021，，3： | 1 |
| 16 | Distribution of natural and anthropogenic thallium in the soils in an industrial pyrite slag disposing area 显示文摘 | Yang Chunxia Chen Yongheng Peng Ping an | 2005 | The Science of the Total Environment2005，341，： | 1 |
| 17 | Surface modification of PET films by atmospheric pressure plasma-induced acrylic acid inverse emulsion graft polymerization显示文摘 | Jie Sun Lan Yao Zhiqiang Gao Shujing Peng Chunxia Wang Yiping Qiu | 2010 | Surface & Coatings Technology2010，，24： | 1 |
| 18 | Construction of a eukaryotic expression plasmid for human retina-derived neurotrophin-3显示文摘Neurotrophin-3 (NT-3) can promote the repair of central nervous system and retinal damage. In previous reports, NT-3 has been expressed by viral vectors. However, plasmid vectors have a safer profile compared with viral vectors in clinical studies. This study recombined amplified human retinal NT-3 with a eukaryotic expression plasmid containing green fluorescent protein (GFP) to construct an NT-3 expression plasmid, pEGFP-N1-NT-3. The transfection efficiency 48 hours after pEGFP-N1-NT-3 transfection to 293T cells was 50.06 ± 2.78%. Abundant NT-3-GFP was expressed in 293T cells as observed by fluorescence microscopy, suggesting the construct pEGFP-N1-NT-3 effectively expressed and secreted NT-3-GFP. Secretory vesicles containing NT-3-GFP were observed in a constant location in cells by laser scan confocal microscopy, indicating the expression and secretion processes of NT-3 in eukaryotic cells were in accordance with the physical synthesis processes of secreted proteins. Western blot assay showed that pro-NT-3-GFP had a molecular weight of 56 kDa, further confirming NT-3-GFP expression. At 48 hours after transfection, the concentration of NT-3 in culture medium was 22.3 ng/mL, suggesting NT-3 produced by pEGFP-N1-NT-3 was efficiently secreted. This study constructed a human retinal-derived NT-3 eukaryotic expression plasmid that efficiently expressed and secreted NT-3. | Chunxia Peng Xiaobei Yin Mengda Li Ting He Genlin Li | 2013 | Neural Regeneration Research2013，8，11： | 1 |
| 19 | A dynamic balanced flow for filtering point-sampled geometry显示文摘 | Chunxia Xiao Yongwei Miao Shu Liu Qunsheng Peng | 2006 | The Visual Computer2006，，3： | 1 |
| 20 | Blog opinion re- trieval based on topic-opinion mixture Model 显示文摘 | JIANG Peng ZI4ANG Chunxia YANC Qing | 2010 | LectureNotes in Computer Science2010，，： | 1 |
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