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| 1 | Construction of a eukaryotic expression plasmid for human retina-derived neurotrophin-3显示文摘Neurotrophin-3 (NT-3) can promote the repair of central nervous system and retinal damage. In previous reports, NT-3 has been expressed by viral vectors. However, plasmid vectors have a safer profile compared with viral vectors in clinical studies. This study recombined amplified human retinal NT-3 with a eukaryotic expression plasmid containing green fluorescent protein (GFP) to construct an NT-3 expression plasmid, pEGFP-N1-NT-3. The transfection efficiency 48 hours after pEGFP-N1-NT-3 transfection to 293T cells was 50.06 ± 2.78%. Abundant NT-3-GFP was expressed in 293T cells as observed by fluorescence microscopy, suggesting the construct pEGFP-N1-NT-3 effectively expressed and secreted NT-3-GFP. Secretory vesicles containing NT-3-GFP were observed in a constant location in cells by laser scan confocal microscopy, indicating the expression and secretion processes of NT-3 in eukaryotic cells were in accordance with the physical synthesis processes of secreted proteins. Western blot assay showed that pro-NT-3-GFP had a molecular weight of 56 kDa, further confirming NT-3-GFP expression. At 48 hours after transfection, the concentration of NT-3 in culture medium was 22.3 ng/mL, suggesting NT-3 produced by pEGFP-N1-NT-3 was efficiently secreted. This study constructed a human retinal-derived NT-3 eukaryotic expression plasmid that efficiently expressed and secreted NT-3. | Chunxia Peng Xiaobei Yin Mengda Li Ting He Genlin Li | 2013 | Neural Regeneration Research2013，8，11： | 1 |
| 2 | Pegylated Liposomal Doxorubicin as a Single Agent or as Combination Therapy with Carboplatin in Patients with Recurrent or Refractory Epithelial Ovarian Cancer显示文摘OBJECTIVE Pegylated liposomal doxorubicin (PLD;CAELYX^(?)), a novel formulation of doxorubicin with enhancedtherapeutic efficacy and reduced toxicity, has demonstratedimproved progression-free survival in recurrent or refractoryovarian cancer. The objective of this open-label, noncomparative,observational study was to determine the efficacyand safety of PLD monotherapy or combination therapy withcarboplatin for patients with recurrent or refractory ovariancancer.METHODS Sixty-two patients with recurrent or refractoryovarian cancer who completed a platinum-based chemotherapyregimen and demonstrated platinum sensitivity for first-linetreatment at least 6 months prior to study entry were enrolledin 20 centers in China. PLD was given as monotherapy (50mg/m^2 infused over 60 minutes) or as combination therapy(30 mg/m^2 1-hour infusion) with carboplatin (area under thecurve 5 mg.min/mL 1-hour infusion) on day 1 every 28 daysfor 4 cycles. The primary endpoint was objective response (OR)rate or CA-125 level. Secondary endpoints included time toresponse, time-to-progression, health-related quality of life, andsafety.RESULTS Overall, 48% of the 62 evaluable patients achieveda confirmed OR. More patients receiving PLD and carboplatinachieved an OR vs the PLD monotherapy group (63% vs. 37%).The median time to response and disease progression was58.5 days and 56.0 days, respectively. Overall and drug-relatedadverse events were reported for 39% and 34%, respectively.The most commonly reported adverse events were stomatitis(22.6%) and palmar-plantar erythroderma (9.7%). Two deathswere reported.CONCLUSION PLD is an effective and well tolerated agentin women with recurrent or refractory epithelial ovarian cancer. | Beihua Kong Yunong Gao Lingying Wu Ziting Li Yile Chen Mengda Li Yongliang Gao Ding ga Zhilan Peng KengShen | 2009 | Clinical oncology and cancer resexreh2009，6，6： | 0 |
| 3 | Ultrasensitive detection of IgE levels based on magnetic nanocapturer linked immunosensor assay for early diagnosis of cancer显示文摘Rapid and accurate detection of immunoglobulin E(Ig E) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of Ig E from patient serum based on Fe3 O4@Si O2-NTA@026 sdab as the capture probe and multiple horseradish peroxidase(HRP)-labeled antibodies linked gold nanoparticles(Au NPs) as chemiluminescence(CL) signal amplifier for ultrasensitive detection of total Ig E. Results showed that the limit of detection of our immunosensor system in serum samples was 0.03 k U/L, which is lowest in comparison with current methods, and far lower than that of Immuno CAP for Ig E detection(0.1 k U/L). Furthermore, our immunosensor possessed satisfied repeatability and accuracy, as well as good stability. In comparison with the Immuno CAP for the quantitative detection of Ig E, highly consistent results were achieved in 20 serum samples. Specially, this method was also successfully utilized for assessing the Ig E traces in breast cancer patients,which provides a new idea for the diagnosis of early cancer. Therefore, we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various Ig E-related diseases. | Yongxin Liu Mengda Cao Zhongxi Huang Changmin Yu Naidi Yang Qiong Wu Liang Shi Wenjie Duan Yi Zhu Jifu Wei Lin Li Wei Huang | 2022 | Chinese Chemical Letters2022，33，4： | 0 |
| 4 | 利用H3、H4组蛋白系统突变库解析生物组蛋白的功能显示文摘文章简介目前,组蛋白修饰酶的研究已表明,组蛋白的表观修饰与生长、发育、癌症等存在密切联系。但由于非组蛋白底物的存在和组蛋白多位点修饰,对组蛋白本身某一修饰功能的理解仍极具挑战,且组蛋白基因在多细胞真核生物中呈现多拷贝和多分布等复杂特征,这极大地阻碍了以组蛋白为核心的表观遗传学发展。 | Weimin Zhang Xuedi Zhang Zhaoyu Xue Yijie Li Qing Ma Xiangle Ren Jiaying Zhang Songhua Yang Lijuan Yang Menghua Wu Mengda Ren Rongwen Xi Zheng Wu Ji-Long Liu Erika Matunis 戴俊彪 高冠军 | 2020 | 科学新闻2020，，2： | 0 |
| 5 | Wideband Polarization Reconfigurable Dielectric Resonator Antenna显示文摘A wideband polarization reconfigurable dielectric resonator antenna excited by quasilumped quadrature coupler( QLQC) is proposed. By adjusting the value of varactor diode on the QLQC feed network,both the wideband LP and CP dielectric resonator antennas( DRA) modes can be achieved. By selecting a different feed port,left-and right-handed CP conversion can be realized.It is found that the 10 dB impedance bandwidth of the LP and CP modes are 10. 1% and 44. 9%,respectively.For the CP mode,a very wide 3 dB axial ratio bandwidth of 37. 7% can be obtained which is much larger than that of the microstrip patch counterpart( 3. 8%). It is worth mentioning that the wideband CP DRA has a stable broadside radiation pattern across the whole operating band where the boresight gain is larger than 3 dBi. | Bin Li Ying Zhou Mengda Wu Yuxuan Xia Yong Liu Xin Lyu | 2017 | Journal of Beijing Institute of Technology2017，26，4： | 0 |
| 6 | Effect of Sintering Time on the Mechanical and Corrosion Behavior of Zn-Mg Composites with a Core-Shell Structure Prepared by SPS显示文摘Zn-10 Mg composite with a core-shell structure was prepared by spark plasma sintering(SPS)technology,and a systematic study of the microstructure and properties has been conducted for different sintering times.The shell layer dominated by the hard MgZn2 phase thickens with the increase in sintering time,which has a positive effect on the mechanical and degradation properties of the material.The sample sintered for 20 min(T-20)has the best mechanical properties,with a compressive strength of 226 MPa and a compression rate of 6.5%.The corrosion resistance of samples increases as the sintering time prolongs,while the hydrogen evolution volume and pH value decrease in the immersion experiment.Furthermore,the increase in the shell thickness significantly reduces the corrosion rate,which is attributed to the weakening of the galvanic corrosion reaction between the Mg core and the MgZn2 shell.Therefore,composite with unique core-shell structure provides an advanced design idea for degradable biomaterials,and a reasonable control of sintering time can provide the optimal design strategy. | Zeqin Cui Lei Zhou Xiaohu Hao Mengda Luo Wenxian Wang Jianzhong Wang Weiguo Li | 2023 | Acta Metallurgica Sinica(English Letters)2023，36，8： | 0 |
| 7 | Performance Analysis of Cross⁃Site Scripting Based on Natural Language Processing显示文摘With the acceleration of network communication in the 5G era,the volume of data communication in cyberspace has increased unprecedentedly.The speed of data transmission will accelerate.Subsequently,the security of network communication data becomes more and more serious.Among them,malicious cross⁃site scripting leading to the leakage of user information is very serious.This article uses URL attribute analysis method and YARA rule to process data for cross⁃site scripting based on the long short⁃term memory(LSTM)characteristics of LSTM model.The results show that the LSTM classification model adopted in this paper has higher recall rate and F1⁃score than other machine learning methods,which proves that the method adopted in this paper is feasible. | Mengda Xu Luqun Li | 2022 | Journal of Harbin Institute of Technology(New Series)2022，29，4： | 0 |
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