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| 1 | Grain nucleation and growth behavior of a Sn-Pb alloy affected by direct current: An in situ investigation显示文摘In situ synchrotron X-ray radiography was used to study the effect of direct current(DC) on the grain nucleation and growth of Sn-50 wt.%Pb alloy. The results showed that applying DC adequately during solidification could effectively enhance the grain nucleation and inhibit its growth. Imaging of comparative experiments with varying DC intensity indicated that the final grain size, determined by the competition between grain nucleation and growth, was sensitively dependent on the DC intensity. It was found that the average grain size was decreased from 1632 to 567 μm with DC density of 1.5 A/mm^2 compared to the case without DC. Beyond this value, raising the current density may cause a significant decrease in the nucleation rate, and thus lead to a coarsening of the grain structure. | Fenfen Yang Zongning Chen Fei Cao Rong Fan Huijun Kang Wanxia Huang Qingxi Yuan Tiqiao Xiao Yanan Fu Tongmin Wang | 2017 | Journal of Materials Science & Technology2017，33，10： | 5 |
| 2 | Effects of L-3-n-butylphthalide on caspase-3 and nuclear factor kappa-B expression in primary basal forebrain and hippocampal cultures after beta-amyloid peptide 1-42 treatment显示文摘BACKGROUND:L-3-n-butylphthalide(L-NBP) can inhibit phosphorylation of tau protein and reduce the neurotoxicity of beta-amyloid peptide 1-42(Aβ_(1-42)). OBJECTIVE:To observe the neuroprotective effects of L-NBP on caspase-3 and nuclear factor kappa-B(NF-κB) expression in a rat model of Alzheimer's disease. DESIGN,TIME AND SETTING:A cell experiment was performed at the Central Laboratory of Provincial Hospital affiliated to Shandong University between January 2008 and August 2008. MATERIALS:L-NBP(purity>98%) was provided by Shijiazhuang Pharma Group NBP Pharmaceutical Company Limited.Aβ_(1-42),3-[4,5-dimethylthiazolo-2]-2,5 iphenyltetrazolium bromide(MTT),and rabbit anti-Caspase-3 polyclonal antibody were provided by Cell Signaling, USA;goat anti-choactase and rabbit anti-NF-κB antibodies were provided by Santa Cruz,USA. METHODS:Primary cultures were generated from rat basal forebrain and hippocampal neurons at 17 or 19 days of gestation.The cells were assigned into five groups:the control group,the Aβ_(1-42) group(2μmol/ L),the Aβ_(1-42) + 0.1μmol/L L-NBP group,the Aβ_(1-42)+ 1μmol/L L-NBP group,and the Aβ_(1-42)+ 10μmol/L L-NBP group.The neurons were treated with Aβ_(1-42)(2μmol/L) alone or in combination with L-NBP(0.1,1,10μmol/L) for 48 hours.Cells in the control group were incubated in PBS. MAIN OUTCOME MEASURES:Morphologic changes were evaluated using inverted microscopy, viability using the MTT method,and the changes in caspase-3 and NF-κB expression using Western blot. RESULTS:Induction with Aβ_(1-42) for 48 hours caused cell death and soma atrophy,and increased caspase-3 and NF-κB expression(P<0.05).L-NBP blocked these changes in cell morphology, decreased caspase-3 and NF-κB expression(P<0.05),and improved cell viability,especially at the high dose(P<0.05). CONCLUSION:Aβ_(1-42) is toxic to basal forebrain and hippocampal primary neurons;L-NBP protects against this toxicity and inhibits the induction of caspase-3 and NF-κB expression. | Ruixia Wang Yong Zhang Liangliang Jiang Guozhao Ma Qingxi Fu Jialong Li Peng Yan Lunqian Shen Yabo Feng Chunxia Li Zaiying Pang Yuanxiao Cui Chunfu Chen Yifeng Du Zhaokong Liu | 2009 | Neural Regeneration Research2009，4，4： | 3 |
| 3 | Mild hypothermia effects on matrix metalloproteinase-9 expression in the perihematomal region of rats following experimental intracerebral hemorrhage显示文摘BACKGROUND: Matrix metalloproteinase-9 (MMP-9) expression increases with intracerebral hemorrhage, and participates in the pathophysiological processes of secondary brain injury after intracerebral hemorrhage. OBJECTIVE: To investigate the effects of mild hypothermia on MMP-9 expression and brain edema in the perihematomal region of experimental intracerebral hemorrhage rats. DESIGN, TIME AND SETTING: The randomized, controlled experiment was performed at the Central Laboratory of Shandong Provincial Hospital between May and September 2007. MATERIALS: Seventy-two, Wistar, male rats, 12-weeks old, were used for this study. Rabbit anti-MMP-9 primary antibody was purchased from Boster, China. METHODS: Wistar rats were equally and randomly divided into normothermia and mild hypothermia groups. The two groups each comprised control, 6-hour intracerebral hemorrhage, 24-hour intracerebral hemorrhage, 48-hour intracerebral hemorrhage, 72-hour intracerebral hemorrhage, and 1-week intracerebral hemorrhage subgroups, with six rats in each subgroup. Rat models of intracerebral hemorrhage were established by injecting 100 μL of autologous blood into the rat caudate nucleus. Rats in the mild hypothermia group received four hours of local mild hypothermia immediately following the injection. Intracerebral temperature was maintained at (33 ± 0.5) ℃. Subsequently, intracerebral temperature was spontaneously recovered at 25 ℃. Rats in the control subgroup were not injected with autologous blood and received only with intracerebral hemorrhage. MAIN OUTCOME MEASURES: Brain water content and MMP-9 expression surrounding the hematoma region. RESULTS: MMP-9 expression increased at 6 hours, and brain edema reached a peak at 48 hours after intracerebral hemorrhage. MMP-9 expression was significantly decreased in the mild hypothermia group compared with the normothermia group at each time point (P < 0.05). CONCLUSION: Mild hypothermia can significantly inhibit MMP-9 overexpression and relieve brain edema following intracerebral hemorrhage. | Minzhong Wang Xiaoyan Lin Xueping Liu Qingxi Fu Suming Zhang | 2008 | Neural Regeneration Research2008，3，7： | 2 |
| 4 | Novel azeotropic distillation process for synthesizing nanoscale powders of yttria doped ceria electrolyte显示文摘 | Shaowu Zha Qingxi Fu Yin Lang | 2001 | Materials Letters2001，47，2： | 1 |
| 5 | Study on diffusion behavior and microstructural evolution of Al/Cu bimetal interface by synchrotron X-ray radiography显示文摘 | Tongmin Wang Fei Cao Peng Zhou Huijun Kang Zongning Chen Yanan Fu Tiqiao Xiao Wanxia Huang Qingxi Yuan | 2014 | Journal of Alloys and Compounds2014，，： | 1 |
| 6 | Novel azeotropic distillation process for synthesizing nanoscale powders of yttria doped ceria electrolyte显示文摘 | Zha Shaowu Fu Qingxi | 2001 | Materials Letters2001，47，2： | 1 |
| 7 | Effects of hypoxic preconditioning on the changes of expression of neuroglobin mRNA and labeled positive cells following cerebral ischemia in gerbils显示文摘BACKGROUND: Neuroglobin (NGB), as newly discovered the third member of the globin family binding oxygen, mainly exists in brain of human beings and vertebrates, and it is closely correlated with the oxygen supply in brain. OBJECTIVE: To observe the changes of the expression of neuroglobin and number of positive cells labeled immunohistochemically following cerebral ischemia in gerbils after hypoxic preconditioning. DESIGN: A complete randomized grouping design and controlled experiment. SETTING: Department of Neurology, Department of Anesthesia, Shandong Provincial Hospital, Shandong University. MATERIALS: Sixty-six adult male Mongolian gerbils of clean degree, about 50-65 g, at an average of 57.5 g were provided by the Experimental Animal Center of Capital Medical University [certificate number of animal quality:SCXK(Beijing)2000-0012]. TRNzil (Tianwei Shidai Company, Beijing), polymerase chain reaction (PCR) primers (synthetized by Invitrogen Company, Shanghai); reverse transcription-PCR (RT-PCR) one-step kit (Toyobo Company); PCR instrument (GeneAmp PCR System 240); mice brain NGB monoclonal antibody (Academy of Military Medical Sciences); DAB (Zhongshan Company, Beijing). METHEDS: The study was completed from December 2004 to June 2005 in Shandong Provincial Hospital. ① The 66 gerbils were randomly divided into sham-operated group (n =6), cerebral ischemia group (n =30) and hypoxic preconditioning group (n =30). The gerbils in the hypoxic preconditioning group were put in the environment which contained O2 (0.08 in volume fraction) and N2 (0.92 in volume fraction) at temperature of 25 ℃ for 2 hours. After 5 hours, the gerbils in the hypoxic preconditioning group and cerebral ischemia group were anesthetized, then bilateral common carotid arteries were ligated. In the sham-operated group, bilateral common carotid arteries were only isolated without ligation and hypoxic preconditioning. ② At 1, 5, 10, 30 and 60 minutes after cerebral ischemia, the expression of NGB mRNA was detected with RT-PCR, and the number of NGF positive cells was counted with immunohistochemical staining. The NGB mRNA expression and number of NGB positive cells at different time points after ischemia were compared between the gerbils treated with and without hypoxic preconditioning. MAIN OUTCOME MEASURES: NGB mRNA expression and number of NGB positive cells at 1, 5, 10, 30 and 60 minutes after cerebral ischemia. RESULTS: All the 66 Mongolian gerbils were involved in the analysis of results. ① Results of NGB mRNA: In the cerebral ischemia group, the NGB mRNA expression began to increase at 1 minute after cerebral ischemia, but had no obvious difference as compared with that in the sham-operated group (P > 0.05), that at 5 minutes was obviously higher than that in the sham-operated group (0.951±0.034, 0.597±0.008, P < 0.05), it decreased gradually 10, 30 and 60 minutes after cerebral ischemia, but had no obvious difference as compared with that in the sham-operated group (P > 0.05). In the hypoxic preconditioning group, the NGB mRNA expression increased rapidly 1 minute after cerebral ischemia, which was obviously higher than that in the cerebral ischemia group (0.641±0.010, 0.618±0.015, P < 0.05), and the expressions at 5, 10 and 30 minutes were still obviously higher than those in the cerebral ischemia group (0.995±0.020 vs. 0.951±0.034; 0.941±0.010 vs. 0.615±0.018; 0.642±0.010 vs. 0.608±0.010, P < 0.05-0.01), whereas the expression at 60 minutes were not obviously different from that in the cerebral ischemia group (P > 0.05). ② Number of NGB positive cells: The numbers of NGB positive cells at 1, 10 and 30 minutes after cerebral ishcemia in the hypoxic preconditioning group [(50.2±3.3), (67.2±3.3), (35.0±4.3) cells] were obviously more than those in the cerebral ischemia group [(33.0±2.1), (60.5±1.9), (23.3±3.1) cells, P < 0.05-0.01], whereas those at 5 and 60 minutes had no obvious differences between the two groups (P > 0.05). CONCLUSION: Hypoxic preconditioning can rapidly accelerate the expression of NGB mRNA following cerebral ischemia, and it plays its neuroprotective role in hypoxic preconditioning. | Yong Zhang Yanqun Chang Zhenfang Liu Qingxi Fu Xiao Zhang | 2006 | Neural Regeneration Research2006，1，5： | 0 |
| 8 | Association between serotonin transporter gene polymorphisms and non-lesional temporal lobe epilepsy in a Chinese Han population显示文摘Serotonin (5-hydroxytryptamine, 5-HT) influences the cortical and subcortical excitatory/inhibitory balance and participates in the pathophysiological processes of epilepsy. The serotonin transporter (5-HTT) is the most important factor in serotonin inactivation. We tested whether 5-HTT polymorphisms are involved in the pathogenesis of epilepsy in Chinese Han population. We did not find a significant difference in the frequencies of genotypes and alleles in the 5-HTT gene-linked polymorphic region (5-HTTLPR) in patients with non-lesional temporal lobe epilepsy and normal controls (P > 0.05). Frequencies of the 5-HTT intron 2 variable number tandem repeat (5-HTTVNTR) 12/12 genotype and allele 12 were higher in the patients with non-lesional temporal lobe epilepsy than normal controls (P < 0.01). The odds ratio of affecting non-lesional temporal lobe epilepsy was 1.435 (95% CI, 1.096-1.880) in patients carrying allele 12 (P < 0.05). Although the 5-HTTLPR may not be a genetic locus of non-lesional temporal lobe epilepsy in Chinese Han population, allele 12 in the 5-HTTVNTR may correlate with non-lesional temporal lobe epilepsy. The Stin2.12 allele and 12/12 genotype could be predisposing to non-lesional temporal lobe epilepsy. | Fengyuan Che Youyi Wei Xueyuan Heng Qingxi Fu Jianzhang Jiang | 2010 | Neural Regeneration Research2010，5，16： | 0 |
| 9 | Accurate reconstruction algorithm for bilateral differential phase signals显示文摘Background The differential phase computed tomography(DP-CT)imaging is a kind of X-ray-phase-based CT methods,which is widely used in various X-ray imaging laboratories.At present,a collection of projections with the horizontal direc-tion(perpendicular to the sample rotation axis)DP information is often acquired in DP-CT.In an ideal experiment,if the background is zero,then we can accurately reconstruct the information of each direction of the sample with horizontal data only.Actually,background information will produce streaking artifacts to affect the resolution in directions other than the horizontal direction.Purpose To mitigate the streaking artifacts in the conventionally reconstructed DP-CT images.Method This study develops a novel analytical DP-CT reconstruction framework by jointly using the bilateral DP information obtained along two perpendicular directions.In addition,a new data acquisition scheme is also proposed to quickly acquire the bilateral DP signal without the need of changing the direction of refraction signal acquisition.Results Experimental results demonstrate that this new algorithm is able to greatly reduce the streaking artifacts on DP-CT images. | Qili He Yan Wang Panyun Li Chunxia Yao Jin Zhang Tianyu Fu Kai Zhang Qingxi Yuan Wanxia Huang Shanfeng Wang Peiping Zhu Peng Liu | 2021 | Radiation Detection Technology and Methods2021，5，3： | 0 |
| 10 | Diazoxide preconditioning antagonizes cytotoxicity induced by epileptic seizures显示文摘Diazoxide, an activator of mitochondrial ATP-sensitive potassium channels, can protect neurons and astrocytes against oxidative stress and apoptosis. In this study, we established a cellular model of epilepsy by culturing hippocampal neurons in magnesium-free medium, and used this to investigate effects of diazoxide preconditioning on the expression of inwardly rectifying potassium channel (Kir) subunits of the ATP-sensitive potassium. We found that neuronal viability was significantly reduced in the epileptic cells, whereas it was enhanced by diazoxide preconditioning. Double immunofluorescence and western blot showed a significant increase in the expression of Kir6.1 and Kir6.2 in epileptic cells, especially at 72 hours after seizures. Diazoxide pretreatment completely reversed this effect at 24 hours after seizures. In addition, Kir6.1 expression was significantly upregulated compared with Kir6.2 in hippocampal neurons after seizures. These findings indicate that diazoxide pretreatment may counteract epileptiform discharge-induced cytotoxicity by suppressing the expression of Kir subunits. | Qingxi Fu Zhiqing Sun Jinling Zhang Naiyong Gao Faying Qi Fengyuan Che Guozhao Ma | 2013 | Neural Regeneration Research2013，8，11： | 0 |
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