|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Development of composite PLGA microspheres containing exenatide-encapsulated lecithin nanoparticles for sustained drug release显示文摘This study aimed to prepare poly(D, L-lactic-co-glycolic acid) microspheres(PLGA-Ms)by a modified solid-in-oil-in-water(S/O/W) multi-emulsion technique in order to achieve sustained release with reduced initial burst and maintain efficient drug concentration for a prolonged period of time. Composite PLGA microspheres containing exenatideencapsulated lecithin nanoparticles(Ex-NPs-PLGA-Ms) were obtained by initial fabrication of exenatide-loaded lecithin nanoparticles(Ex-NPs) via the alcohol injection method,followed by encapsulation of Ex-NPs into PLGA microspheres. Compared to Ms prepared by the conventional water-in-oil-in-water(W/O/W) technique(Ex-PLGA-Ms), Ex-NPs-PLGAMs showed a more uniform particle size distribution, reduced initial burst release, and sustained release for over 60 d in vitro. Cytotoxicity studies showed that Ms prepared by both techniques had superior biocompatibility without causing any detectable cytotoxicity.In pharmacokinetic studies, the effective drug concentration was maintained for over 30 d following a single subcutaneous injection of two types of Ms formulation in rats, potentially prolonging the therapeutic action of Ex. In addition, administration of Ex-NPs-PLGA-Ms resulted in a more smooth plasma concentration-time profile with a higher area under the curve(AUC) compared to that of Ex-PLGA-Ms. Overall, Ex-NPs-PLGA-Ms prepared by the novel S/O/W method could be a promising sustained drug release system with reduced initial burst release and prolonged therapeutic efficacy. | Ni Dong Chune Zhu Junhuang Jiang Di Huang Xing Li Guilan Quan Yang Liu Wen Tan Xin Pan Chuanbin Wu | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，3： | 7 |
| 2 | Comparison of Clinical and Epidemiological Characteristics of Asymptomatic and Symptomatic SARS-CoV-2 Infection in Children显示文摘To understand the epidemiological and clinical features of the symptomatic and asymptomatic pediatric cases of COVID-19,we carried out a prospective study in Shanghai during the period of January 19 to April 30,2020.A total of 49 children(mean age 11.5±5.12 years)confirmed with SARS-CoV-2 infection were enrolled in the study,including 11(22.4%)domestic cases and 38(77.6%)imported cases.Nine(81.8%)local cases and 12(31.6%)imported cases had a definitive epidemiological exposure.Twenty-eight(57.1%)were symptomatic and 21(42.9%)were asymptomatic.Neither asymptomatic nor symptomatic cases progressed to severe diseases.The mean duration of viral shedding for SARS-CoV-2 in upper respiratory tract was 14.1±6.4 days in asymptomatic cases and 14.8±8.4 days in symptomatic cases(P>0.05).Forty-five(91.8%)cases had viral RNA detected in stool.The mean duration of viral shedding in stool was 28.1±13.3 days in asymptomatic cases and 30.8±18.6 days in symptomatic participants(P>0.05).Children<7 years shed viral RNA in stool for a longer duration than school-aged children(P<0.05).Forty-three(87.8%)cases had seropositivity for antibodies against SARS-CoV-2 within 1–3 weeks after confirmation with infection.In conclusion,asymptomatic SARS-CoV-2 infection may be common in children in the community during the COVID-19 pandemic wave.Asymptomatic cases shed viral RNA in a similar pattern as symptomatic cases do.It is of particular concern that asymptomatic individuals are potentially seed transmission of SARS-CoV-2 and pose a challenge to disease control. | Jiehao Cai Xiangshi Wang Jun Zhao Yanling Ge Jin Xu He Tian Hailing Chang Aimei Xia Jiali Wang Jinqiang Zhang Zhongqiu Wei Jingjing Li Chuning Wang Jianshe Wang Qirong Zhu Xiaowen Zhai Mei Zeng | 2020 | Virologica Sinica2020，35，6： | 4 |
| 3 | Single-cell transcriptomic profiling unravels the adenoma-initiation role of protein tyrosine kinases during colorectal tumorigenesis显示文摘The adenoma-carcinoma sequence is a well-accepted roadmap for the development of sporadic colorectal cancer.However,cellular heterogeneity in aberrant epithelial cells limits our understanding of carcinogenesis in colorectal tissues.Here,we performed a single-cell RNA sequencing survey of 54,788 cells from patient-matched tissue samples,including blood,normal tissue,para-cancer,polyp,and colorectal cancer. | Xiaobo Zheng Jinen Song Chune Yu Zongguang Zhou Xiaowei Liu Jing Yu Guangchao Xu Jiqiao Yang Xiujing He Xin Bai Ya Luo Yu Bao Huifang Li Lie Yang Mingqing Xu Nan Song Xiaodong Su Jie Xu Xuelei Ma Hubing Shi | 2022 | Signal Transduction and Targeted Therapy2022，7，3： | 2 |
| 4 | Spectroscopic study of conformation changes of bovine serum albumin in aqueous environment显示文摘The secondary structural changes of bovine serum albumin (BSA) aqueous solutions with and without calcium cations were investigated by attenuated total reflection-Fourier transform infrared (ATR-FTIR) technology. The spectra of BSA solution and BSA dry powder were mainly reflected the formation of hydrogen bonds between water and BSA. Further investigation indicated that the concentrations of calcium cations in BSA aqueous solution also affected the secondary structural change of the protein. Amide I band was red shifted and amide II band was blue shifted in aqueous environment compared with the dry BSA powder, and the addition of calcium cations further changed the amide bands position, which led to the change of the secondary structure. The result was coinciding with the Raman spectroscopy. | Chune Guo Xiaomi Guo Wubo Chu Nan Jiang He Li | 2019 | Chinese Chemical Letters2019，30，6： | 2 |
| 5 | Overweight, obesity, and their associations with insulin resistance and β -cell function among Chinese: a cross-sectional study in China显示文摘 | Gang Chen Chune Liu Jin Yao Qiqin Jiang Nianhui Chen Huibin Huang Jixing Liang Liantao Li Lixiang Lin | 2010 | Metabolism2010，，12： | 1 |
| 6 | Hewei Jiangni granule alleviates visceral hypersensitivity in a rat model of non-erosive reflux disease via transient receptor potential channel signaling显示文摘Objective:To uncover the underlying mechanism of Hewei Jiangni granule(HWJNG)on non-erosive reflux disease(NERD)treatment by examining histological changes,gastrointestinal neurochemicals release and visceral hypersensitivity-related receptor expression in NERD model rats.Methods:A NERD rat model was established via a combination of basal sensitization and acid perfusion.HWJNG treatments at different doses were then administered.Pathological changes to tissues,mast cell(MC)activation,serum levels of esophageal visceral hypersensitivity-related neurochemicals,and transient receptor potential(TRP)receptor mRNA and protein levels were investigated.Results:Compared with the control group,the expression of tryptase in MCs,the changes of intercellular space,and the serum levels of substance P(SP),calcitonin gene-related peptides(CGRP)and proteinaseactivated receptor 2(PAR2)increased in the model group(all P<.05).The expression of TRP vanilloid 1(Trpv1)mRNA decreased in esophagus and dorsal root ganglia(DRG)of the model group(P=.030&P=.013),and the expression of TRP melastatin channel subfamily member 8(Trpm8)mRNA decreased in the esophagus of model group(P<.01).The level of esophageal TRPV1 protein increased in the model group(P<.01)and the level of TRPM8 protein decreased in esophagus and DRG of the model group(both P<.05).Compared with the model group,the serum levels of SP,CGRP,and PAR2 in the mediumdose HWJNG group showed significant decreases(all P<.05).The expression of Trpv1 mRNA in esophagus and DRG of the HWJNG groups and the Omeprazole group remarkably decreased(all P<.05),as was the expression of Trpm8 mRNA in esophagus of the HWJNG groups(all P<.05).Conclusion:HWJNG alleviated visceral hypersensitivity in NERD model rats by regulating TRP-mediated signaling.Our results indicate that HWJNG has potential as a therapeutic agent for NERD. | Jiali Liu Fushun Kou Xiang Tan Yi Dai Chune Xie Xiaohong Li Lei Shi Tangyou Mao Xiaojun Shi Junxiang Li | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，2： | 1 |
| 7 | FTIR-ATR study for adsorption of trypsin in aqueous environment on bare and TiO2 coated ZnSe surfaces显示文摘Undesired adsorption of proteins brings big troubles to marine structures.The settled proteins change the physical and chemical properties of the surfaces,which allow marine fouling organisms to settle down on the structures.Therefore,to understand the adsorption mechanism of proteins is very helpful to find an environment-friendly solution against biofouling.Many approaches have been developed to study protein adsorption,but most of them are insufficient to give the chemical interaction information between proteins and surfaces.Fourier transform infrared spectroscopy with attenuated total reflection(FTIR-ATR)is an efficient,fast and non-destructive method for in situ surface measurement,which greatly minimizes the interference of water to infra red spectra,because of the very small depth of penetration of the evanescent wave.In this paper,an in situ FTIR-ATR technology was used to investigate the adsorption process of trypsin on a bare ZnSe surface and on a TiO2 coated ZnSe surface,and the effect of calcium cation strength and ultraviolet light irradiation on the secondary structure of trypsin were also evaluated.FTIR spectra of trypsin showed that Amide I band red shift and AmideⅡband blue shift in aqueous environment on both surfaces compared with the dry trypsin powder,and the addition of calcium cations further changed the Amide bands position,which indicated that the change of the secondary structure could be interfered by the environment.The hydrogen bond formation between water and trypsin,the interaction between surface and trypsin,the interaction between hydrated calcium cations and trypsin,are major facto rs to change the secondary structure of trypsin,and UV light irradiation also showed its influence for the secondary structure. | Chune Guo Xiaomi Guo Wubo Chu Nan Jiang He Li | 2020 | Chinese Chemical Letters2020，31，1： | 1 |
| 8 | 3D model study on fog over complex terrain part Ⅰ:numerical study显示文摘 | Li Zihua Shi Chune | 1997 | Acta Meteorologica Sinica1997，10，1： | 1 |
| 9 | 3D model study on fog over complex terrain part Ⅰ: numerical study 显示文摘 | Li Zihua Shi Chune | 1997 | Acta Meteor Sinica1997，10，1： | 1 |
| 10 | Removal of nitro- gen by heterotrophic nitrification - aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG -24 显示文摘 | Chune Li Jinshui Yang Xin Wang | 2015 | Bioresource Technology2015，182，： | 1 |
| 11 | Combined superposition effect of hypertension and dyslipidemia on left ventricular hypertrophy显示文摘Background : Hypertension and dyslipidemia are considered reversible risk factors for cardiovascular disease. The purpose of this study was to explore the impact of traditional and nontraditional blood lipid profiles on the risk of left ventricular hypertrophy(LVH) and to explore the superposition effect of dyslipidemia combined with hypertension.Methods : Data on 9134 participants(53.5 ±10.3 years old) from the Northeast China Rural Cardiovascular Health Study(NCRCHS) were statistically analyzed. The blood lipid profile was measured by total cholesterol(TC), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein cholesterol(HDL-C), total glyceride(TG), and calculated nontraditional blood lipid indices including non-HDL-C, atherosclerosis index(AI), TC/HDL-C, and residual cholesterol(RC).Results : After the adjustment of age and gender, the odds ratios(ORs) of LVH in patients with hypertension, high LDL-C, high non-HDL-C, high AI, and high TC/HDL-C were 3.97(3.31– 4.76), 1.27(1.02– 1.59), 1.21(1.04– 1.39), 1.33(1.15– 1.53), and 1.42(1.22– 1.65), respectively. After full adjustment of potential confounding factors, high AI and TC/HDL-C were associated with LVH rather than traditional blood lipid indices.The combination of hypertension and nontraditional dyslipidemia(defined by high AI and TC/HDL-C) was associated with the highest risk of LVH, especially in participants under 45 years of age. The risk was more significant in men, 5.09-fold and 6.24-fold,respectively, compared with 3.66-fold and 4.01-fold in women.Conclusions : People with dyslipidemia defined by nontraditional blood lipid indices(high AI and high TC/HDL-C) and hypertension were more likely to develop LVH. | Xueyao Zhang Guangxiao Li Chuning Shi Dongyuan Zhang Yingxian Sun | 2022 | Animal Models and Experimental Medicine2022，5，3： | 1 |
| 12 | Biochemical responses to the toxicity of the biocide abamectin on the freshwater snail Physa acuta显示文摘 | Junguo Ma Chune Zhou Yao Li Xiaoyu Li | 2013 | Ecotoxicology and Environmental Safety2013，，： | 1 |
| 13 | Electric and dielectric properties of polyaniline显示文摘 | PingshengHe Xiaohua Qian Chune Li | 1993 | SynthMet1993，5557，： | 1 |
| 14 | Fog Simulations Based on Multi-Model System: A Feasibility Study显示文摘 | Chune Shi Lei Wang Hao Zhang Su Zhang Xueliang Deng Yaosun Li Mingyan Qiu | 2012 | Pure and Applied Geophysics2012，，5： | 1 |
| 15 | Enantioselective Chlorocyclization of Olefinic Amides with 1,3-Dichloro-5,5-Dimethylhydantoin(DCDMH) Catalyzed by(DHQD)2PHAL显示文摘 | ZHOU Qingjun GUO Chao LI Xiwang HE Pei YANG Guichun DONG Chune | 2018 | Wuhan University Journal of Natural Sciences2018，23，3： | 0 |
| 16 | ATR-FTIR study of Bacillus sp. and Escherichia coli settlements on the bare and Al_2O_3 coated ZnSe internal reflection element显示文摘Marine microorganism accumulated on the surface of ships or pipelines would accelerate fouling organisms, such as mussels and barnacles, adhered on the surface. It was significant to understand the bio-interaction between the microorganisms and the surface. Attenuated total reflection(ATR) Fourier transform infrared(FTIR) spectroscopy was used to study the initial stages of marine microorganism adhering to surfaces, because it could probe the microorganism interaction to the surface regardless of the water interference. Bacillus sp. and Escherichia coli were selected to study the initial attachment on different surfaces, because they were typical fouling microorganisms and showed opposite Gram stain results. The assays were conducted respectively in dried and settled bacteria on two different surfaces(ZnSe, Al2 O3 coated on ZnSe). IR spectra of settled bacteria showed amide I band red shift and amide II band blue shift in aqueous environment on both surfaces compared with the dry bacteria. The reasons of amide bands shift were investigated and it was discovered that the hydrogen bond between the water and the protein of the bacteria led to the protein secondary structure change. ATR-FTIR provided an approach to study the attachment process and showed dynamic changing process on the surface, and it could be an appropriate approach to study the interaction between proteins and chemicals. | Rongqiao Chen Chune Guo Wubo Chu Nan Jiang He Li | 2019 | Chinese Chemical Letters2019，30，1： | 0 |
| 17 | Isomerization and bioaccessibility of cypermethrin and fenpropathrin in Pacific oyster during simulated digestion as influenced by domestic cooking methods显示文摘Pyrethroids can be ingested by humans through eating contaminated oysters,which is potentially harmful to health.This study aimed to investigate the effects of raw,steaming,and roasting on cypermethrin(CP)and fenpropathrin(FP)in oysters during simulated digestion.Results showed that the amount of released CP and FP was different from raw(CP:0.617μg·g−1,FP:0.266μg·g−1),steaming(CP:0.498μg·g−1,FP:0.660μg·g−1),and roasting(CP:1.186μg·g−1,FP:0.588μg·g−1)at the end of simulated digestion.The share of cis-CP and low-efficiency CP increased significantly(p<0.05),and the share of high-efficiency trans-CP did not maintain a high level for a long time during simulated digestion.The fluorimetric titration and isothermal titration calorimetry confirmed that CP and FP could spontaneously interact with oyster actin,and CP could bind with oyster actin more tightly than FP.This study reveals that cooking methods affect the binding capacity of CP and FP to oyster tissues and influence the changes of CP and FP in oysters during digestion.Furthermore,the current study provides a reference for assessing the potential harm of pyrethroids in oysters to consumers. | Hangtao Xie Yadan Jiao Tian Li Tuo Zhang Yanyan Zheng Yongkang Luo Yuqing Tan Chune Liu Hui Hong | 2023 | Food Innovation and Advances2023，2，1： | 0 |
| 18 | Alternative polyadenylation-related genetic variants contribute to bladder cancer risk显示文摘Aberrant alternative polyadenylation(APA)events play an important role in cancers,but little is known about whether APA-related genetic variants contribute to the susceptibility to bladder cancer.Previous genome-wide association study performed APA quantitative trait loci(apaQTL)analyses in bladder cancer,and identified 17955 single nucleotide polymorphisms(SNPs).We found that gene symbols of APA affected by apaQTL-associated SNPs were closely correlated with cancer signaling pathways,high mutational burden,and immune infiltration.Association analysis showed that apaQTL-associated SNPs rs34402449 C>A,rs2683524 C>T,and rs11540872 C>G were significantly associated with susceptibility to bladder cancer(rs34402449:OR=1.355,95%confidence interval[CI]:1.159-1.583,P=1.33×10^(−4);rs2683524:OR=1.378,95%CI:1.164-1.632,P=2.03×10^(−4);rs11540872:OR=1.472,95%CI:1.193-1.815,P=3.06×10^(−4)).Cumulative effect analysis showed that the number of risk genotypes and smoking status were significantly associated with an increased risk of bladder cancer(P_(trend)=2.87×10^(−12)).We found that PRR13,being demonstrated the most significant effect on cell proliferation in bladder cancer cell lines,was more highly expressed in bladder cancer tissues than in adjacent normal tissues.Moreover,the rs2683524 T allele was correlated with shorter 3′untranslated regions of PRR13 and increased PRR13 expression levels.Collectively,our findings have provided informative apaQTL resources and insights into the regulatory mechanisms linking apaQTL-associated variants to bladder cancer risk. | Ting Liu Jingjing Gu Chuning Li Mengfan Guo Lin Yuan Qiang Lv Chao Qin Mulong Du Haiyan Chu Hanting Liu Zhengdong Zhang | 2023 | The Journal of Biomedical Research2023，37，6： | 0 |
| 19 | A revisit to MacKay algorithm and its application to deep network compression显示文摘An iterative procedure introduced in MacKay’s evidence framework is often used for estimating the hyperparameter in empirical Bayes.Together with the use of a particular form of prior,the estimation of the hyperparameter reduces to an automatic relevance determination model,which provides a soft way of pruning model parameters.Despite the effectiveness of this estimation procedure,it has stayed primarily as a heuristic to date and its application to deep neural network has not yet been explored.This paper formally investigates the mathematical nature of this procedure and justifies it as a well-principled algorithm framework,which we call the MacKay algorithm.As an application,we demonstrate its use in deep neural networks,which have typically complicated structure with millions of parameters and can be pruned to reduce the memory requirement and boost computational efficiency.In experiments,we adopt MacKay algorithm to prune the parameters of both simple networks such as LeNet,deep convolution VGG-like networks,and residual netowrks for large image classification task.Experimental results show that the algorithm can compress neural networks to a high level of sparsity with little loss of prediction accuracy,which is comparable with the state-of-the-art. | Chune LI Yongyi MAO Richong ZHANG Jinpeng HUAI | 2020 | Frontiers of Computer Science2020，14，4： | 0 |
| 20 | UI layers merger: merging UI layers via visual learning and boundary prior显示文摘With the fast-growing graphical user interface(GUI)development workload in the Internet industry,some work attempted to generate maintainable front-end code from GUI screenshots.It can be more suitable for using user interface(UI)design drafts that contain UI metadata.However,fragmented layers inevitably appear in the UI design drafts,which greatly reduces the quality of the generated code.None of the existing automated GUI techniques detects and merges the fragmented layers to improve the accessibility of generated code.In this paper,we propose UI layers merger(UILM),a vision-based method that can automatically detect and merge fragmented layers into UI components.Our UILM contains the merging area detector(MAD)and a layer merging algorithm.The MAD incorporates the boundary prior knowledge to accurately detect the boundaries of UI components.Then,the layer merging algorithm can search for the associated layers within the components’boundaries and merge them into a whole.We present a dynamic data augmentation approach to boost the performance of MAD.We also construct a large-scale UI dataset for training the MAD and testing the performance of UILM.Experimental results show that the proposed method outperforms the best baseline regarding merging area detection and achieves decent layer merging accuracy.A user study on a real application also confirms the effectiveness of our UILM. | Yunnong CHEN Yankun ZHEN Chuning SHI Jiazhi LI Liuqing CHEN Zejian LI Lingyun SUN Tingting ZHOU Yanfang CHANG | 2023 | Frontiers of Information Technology & Electronic Engineering2023，24，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员