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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Lung recruitment maneuvers in acute respiratory distress syndrom e and facilitating resolution显示文摘 | Barbas C S C | 2003 | Crit Care Med2003，31，： | 2 |
| 3 | Gut microbiota disturbance during antibiotic therapy: a multi-omic approach显示文摘 | Ana Elena Pérez-Cobas María José Gosalbes Anette Friedrichs Henrik Knecht Alejandro Artacho Kathleen Eismann Wolfgang Otto David Rojo Rafael Bargiela Martin von Bergen Sven C Neulinger Carolin D?umer Femke-Anouska Heinsen Amparo Latorre Coral Barbas Jana | 2013 | Gut2013，，11： | 2 |
| 4 | Selection and evolution of high-affinity human anti-viral antibodies显示文摘 | Burton D R | 1996 | Trends Biotechnol1996，14，7： | 1 |
| 5 | Detection of radiolytic hydrocarbons by supercritical fluid extraction and gas chromatographic-mass spectrometric analysis of irradiated cheese显示文摘 | Barba C Calvo M M Herraiz M | 2009 | Food Chemistry2009，114，4： | 1 |
| 6 | Intratumor LAK cell and interleukin-2 therapyof human gliomas显示文摘 | Barba D Saris SC holder C | 1989 | J Neurosurg1989，70，2： | 1 |
| 7 | Morphology of starch in surimi gels 显示文摘 | Couso I Alvarez C Solas M T Barba C | 1998 | Zeitsechnft ftlr Lebensmittel-untersuchung und- Forschung1998，206，1： | 1 |
| 8 | Optimization in sample stacking for the measurement of short chain organic acids in serum of natural rubber latex by capillary electrophoresis显示文摘 | GALLI V BARBAS C | 2003 | Anal Chim Acta2003，482，1： | 1 |
| 9 | Ictal clinical and scalp- EEG findings differentiating temporal lobe epilepsies from temporal 'plus' epilepsies显示文摘 | Barba C Barbati G Minotti L | 2007 | Brain2007，130，： | 1 |
| 10 | Zinc Finger Tools:custom DNA-binding domains for transcription factors and nucleases显示文摘 | Mandell J G Barbas C F 3rd | 2006 | Nucleic Acids Res2006，34，： | 1 |
| 11 | Ictal clinical and scalp- EEG findings differentiating temporal lobe epilepsies from temporal ' plus' epilepsies显示文摘 | Barba C Barbati G Minotti L | 2007 | Brain2007，130，： | 1 |
| 12 | Amino Alcohol Catalyzed Direct Asymmetric Aldol Reactions:Enantionselective Synthesis of Anti-α-fluoroβ-hydroxy Ketones显示文摘 | Fan J H Barbas Ⅲ C F | 2004 | Tetrahedron Lett2004，45，： | 1 |
| 13 | Effect of a protective-ventilation strategy on mortality in the acute respiratory distress syndrome显示文摘 | Amato M B Barbas C S Medeiros D M | 1998 | N Engl J Med1998，338，： | 1 |
| 14 | Effect of topical anti-inflammatory drugs on corneal and limbal wound healing显示文摘 | Barba KR Samy A Lai C | 2000 | J Cataract Refract Surg2000，26，6： | 1 |
| 15 | LC determination of impurities in azithromycin tablets显示文摘 | Miguel L Barbas C | 2003 | J Pharmaceut Biomed2003，33，： | 1 |
| 16 | Enamine-based organocatalysis with proline and diamines: the development of direct catalytic asymmetric Aldol, Mannich, Michael, and Diels-Alder reactions 显示文摘 | Notz W Tanaka F Barbas C F | 2004 | Accounts of Chemical Research2004，，37： | 1 |
| 17 | Phage display of combinatorial antibody libraries显示文摘 | Rader C Barbas C F | 1997 | Curr Opin Biotechnol1997，8，4： | 1 |
| 18 | Optimization of the separation lactic acid enantiomers in body fluids by capillary electrophoresis显示文摘 | Saavedra L Barbas C | 2002 | J Chromatogr B2002，766，2： | 1 |
| 19 | Characterization of coloured compound sobtained by enzymatic extraction of bakery products 显示文摘 | BORRELLI R C MENNELLA C BARBA F | 2003 | Food and Chemical Toxicology2003，41，10： | 1 |
| 20 | Effect of a protective-ventilation strategy on mortality in the acute respiratory distress syndrome显示文摘 | Amato M B P Barbas C S V Medeiros D M | 1998 | N Engl J Med1998，338，： | 1 |
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