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您的检索式：作者名="David C Manns"
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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Reduction of Patulin in Apple Cider by UV Radiation显示文摘 | Dong Qingfang Manns David C Feng Guoping Yue Tianli Churey John J Worobo Randy W | 2010 | Journal of Food Protection2010，，1： | 1 |
| 3 | The economics of end-stage renal disease care in Canada:incentives and impact on delivery of care显示文摘 | Braden J Manns David C | 2007 | Int J Health Care Finance Econ2007，7，： | 1 |
| 4 | Reduction of Patulin in Apple Cider by UV Radiation显示文摘 | Dong Qingfang Manns David C Feng Guoping Yue Tianli Churey John J Worobo Randy W | 2010 | Journal of Food Protection2010，，1： | 1 |
| 5 | Large-scale sequence analysis of avian influ- enza isolates显示文摘 | John C Obenauer Jackie Denson Perdeep K Mehta Xiaoping Su Suraj Mukatira David B Finkelstein Xiequn Xu Jinhua Wang Jing Ma Yiping Fan Ka- ren M Rakestraw Robert G Webster Erich Hoff mann Scott Krauss Jie Zheng Ziwei Zhang Clayton W Naeve | 2006 | Science2006，311，5767： | 1 |
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