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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | A simple and efficient method for DNA extraction from grapevine cultivars andVitis species显示文摘 | Muhammad A. Lodhi Guang-Ning Ye Norman F. Weeden Bruce I. Reisch | 1994 | Plant Molecular Biology Reporter1994，，1： | 1 |
| 3 | Long-term study of somatic embryogenesis from anthers and ovaries of 12 grapevine (Vitis sp.) genotypes显示文摘 | Julie R. Kikkert Michael J. Striem José R. Vidal Patricia G. Wallace John Barnard Bruce I. Reisch | 2005 | In Vitro Cellular & Developmental Biology - Plant2005，，3： | 1 |
| 4 | Biolistic transformation of grapevine using minimal gene cassette technology显示文摘 | Jose R. Vidal Julie R. Kikkert Bruno D. Donzelli Patricia G. Wallace Bruce I. Reisch | 2006 | Plant Cell Reports2006，，8： | 1 |
| 5 | Selection and characterization of ethionine-resistant alfalfa (Medicago sativa L.) cell lines显示文摘 | Dr. Bruce I. Reisch Stanley H. Duke E. T. Bingham | 1981 | Theoretical and Applied Genetics1981，，2： | 1 |
| 6 | A simple and efficient method for DNA extraction from grapevine cultivars andVitis species显示文摘 | Muhammad A. Lodhi Guang-Ning Ye Norman F. Weeden Bruce I. Reisch | 1994 | Plant Molecular Biology Reporter1994，，1： | 1 |
| 7 | A simple and efficient method for DNA extraction from grapevine cultivars andVitis species显示文摘 | Muhammad A. Lodhi Guang-Ning Ye Norman F. Weeden Bruce I. Reisch | 1994 | Plant Molecular Biology Reporter1994，，1： | 1 |
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