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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | QT prolongation is associated with increased mortality in end stage liver disease显示文摘AIM To determine the prevalence of QT prolongation in a large series of end stage liver disease(ESLD) patients and its association to clinical variables and mortality.METHODS The QT interval was measured and corrected for heart rate for each patient,with a prolonged QT cutoff defined as QT > 450 ms for males and QT > 470 ms for females.Multiple clinical variables were evaluated including sex,age,serum sodium,international normalized ratio,creatinine,total bilirubin,beta-blocker use,Model for EndStage Liver Disease(MELD),MELD-Na,and etiology of liver disease. RESULTS Among 406 ESLD patients analyzed,207(51.0%) had QT prolongation. The only clinical variable associated with QT prolongation was male gender(OR = 3.04,95%CI:2.01-4.60,P < 0.001). During the study period,187patients(46.1%) died. QT prolongation was a significant independent predictor of mortality(OR = 1.69,95%CI:1.03-2.77,P = 0.039). In addition,mortality was also associated with viral etiology of ESLD,elevated MELD score and its components(P < 0.05 for all). No significant reversibility in the QT interval was seen after liver transplantation. CONCLUSION QT prolongation was commonly encountered in an ESLD population,especially in males,and served as a strong independent marker for increased mortality in ESLD patients. | Sun Moon Kim Bennet George Diego Alcivar-Franco Charles L Campbell Richard Charnigo Brian Delisle Jonathan Hundley Yousef Darrat Gustavo Morales Samy-Claude Elayi Alison L Bailey | 2017 | World Journal of Cardiology2017，9，4： | 3 |
| 3 | 乳腺癌死亡率下降归功于筛查和辅助治疗的增加,尤其是辅助治疗显示文摘 | Alison L Jones 樊英 | 2005 | 英国医学杂志中文版2005，8，3： | 3 |
| 4 | Effect of Ginkgo biloba (EGb 761) and aspirin on platelet aggregation and platelet function analysis among older adults at risk of cardiovascular disease: a randomized clinical trial显示文摘 | Christopher D Gardner James L Zehnder Alison J Rigby Joel R Nicholus John W Farquhar | 2007 | Blood Coagulation & Fibrinolysis2007，，8： | 2 |
| 5 | The interfacial transition zone(ITZ) between cement paste and aggregate in concrete显示文摘 | SCRIVENER KAREN L CRUMBIE ALISON K | 2004 | Interface Science2004，12，4： | 1 |
| 6 | Accurnulation of an endogenous in hibitors of nitric oxide synthesis in chronic renalfailure显示文摘 | Patric V Anna L Alison C | 1992 | Lancet1992，339，8795： | 1 |
| 7 | Prenatal stress diminishes neurogenesis in the dentate gyrus of juvenile Rhesus monkeys显示文摘 | Christopher L Coe Marian Kramer Boldizsár Czéh Elizabeth Gould Alison J Reeves Clemens Kirschbaum Eberhard Fuchs | 2003 | Biological Psychiatry2003，，10： | 1 |
| 8 | Carnitine: a nutritional, biosynthetic, and functional perspective显示文摘 | Alison S Janos K Charles L H | 2004 | Mol Aspects Med2004，25，： | 1 |
| 9 | International physical activity questionnaire: 12-country reliability and validity显示文摘 | CORA L CRAING ALISON L SJOSTROM M | 2003 | Med Sci Sports Exerc2003，35，8： | 1 |
| 10 | Cancer stem cells:problems for therapy显示文摘 | Alison M R Lira S M Nieholson L J | 2011 | JPathol2011，223，2： | 1 |
| 11 | Tolerance of Antarctic cyanobacterial mats to enhanced UV radiation显示文摘 | ALISON L G ALISTAIR W M PEDRO O M | 2001 | FEMS Microbiology Ecology2001，37，： | 1 |
| 12 | The new stem cell biology:something for every显示文摘 | Preston S L Alison M R Forbes S J | 2003 | J Clin Pathol:Mol Pathol2003，56，： | 1 |
| 13 | Hepatotoxicity in an African antiretroviral therapy cohort: the effect of tuberculosis and hepatitis B显示文摘 | Christopher J Hoffmann Salome Charalambous Chloe L Thio Desmond J Martin Lindiwe Pemba Katherine L Fielding Gavin J Churchyard Richard E Chaisson Alison D Grant | 2007 | AIDS2007，，10： | 1 |
| 14 | Construction of commutative filters for les on unstructured meshes 显示文摘 | Alison L Marsden Vasilyev O V Parvoz Moin | 2002 | Journal of Computational Physics2002，175，2： | 1 |
| 15 | Galectin-3 regulates myofibroblast activation and hepatic fibrosis显示文摘 | Neil C Alison C Sarah L | 2006 | Proc Natl Acad Sci U S A2006，103，13： | 1 |
| 16 | Influence of grain morphology on electron transport in dye sensitized nanocrystalline solar cells 显示文摘 | Cass M J Qiu F L Alison W B | 2003 | J Phys Chem B2003，107，： | 1 |
| 17 | The use of willingness-to-pay approaches in mammal conservation显示文摘 | Alison C Emma J V H | 2001 | Mammal Bev2001，31，2： | 1 |
| 18 | Application strategies:An organizational perspective显示文摘 | Rynes Sara L and Barber Alison E | 1990 | The Academy of Management Review1990，15，2： | 1 |
| 19 | Cancer stem cells: in the line of fire显示文摘 | Alison M R Lin W R Lira S M L | 2012 | Cancer Treat Rev2012，38，6： | 1 |
| 20 | Application Strategies: An organizational Perspective 显示文摘 | Rynes Sara L Barber Alison E | 1990 | The academy of Management Review1990，，2： | 1 |
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