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| 1 | Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells显示文摘RNase III DROSHA is upregulated in multiple cancers and contributes to tumor progression by hitherto unclear mechanisms.Here,we demonstrate that DROSHA interacts withβ-Catenin to transactivate STC1 in an RNA cleavage-independent manner,contributing to breast cancer stem-like cell(BCSC)properties.DROSHA mRNA stability is enhanced by N6-methyladenosine(m^(6)A)modification which is activated by AURKA in BCSCs.AURKA stabilizes METTL14 by inhibiting its ubiquitylation and degradation to promote DROSHA mRNA methylation.Moreover,binding of AURKA to DROSHA transcript further strengthens the binding of the m^(6)A reader IGF2BP2 to stabilize m^(6)A-modified DROSHA.In addition,wild-type DROSHA,but not an m^(6)A methylation-deficient mutant,enhances BCSC stemness maintenance,while inhibition of DROSHA m^(6)A modification attenuates BCSC traits.Our study unveils the AURKA-induced oncogenic m^(6)A modification as a key regulator of DROSHA in breast cancer and identifies a novel DROSHA transcriptional function in promoting the BCSC phenotype. | Fei Peng Jie Xu Bai Cui Qilan Liang Sai Zeng Bin He Hong Zou Manman Li Huan Zhao Yuting Meng Jin Chen Bing Liu Shasha Lv Peng Chu Fan An Zifeng Wang Junxiu Huang Yajing Zhan Yuwei Liao Jinxin Lu Lingzhi Xu Jin Zhang Zhaolin Su Zhiguang Li Fangjun Wang Eric W-FLam Quentin Liu | 2021 | Cell Research2021，31，3： | 9 |
| 2 | Optimal operation of cold–heat–electricity multi-energy collaborative system based on price demand response显示文摘In a multi-energy collaboration system, cooling, heating, electricity, and other energy components are coupled to complement each other. Through multi-energy coordination and cooperation, they can significantly improve their individual operating efficiency and overall economic benefits. Demand response, as a multi-energy supply and demand balance method, can further improve system flexibility and economy. Therefore, a multi-energy cooperative system optimization model has been proposed, which is driven by price-based demand response to determine the impact of power-demand response on the optimal operating mode of a multi-energy cooperative system. The main components of the multi-energy collaborative system have been analyzed. The multi-energy coupling characteristics have been identified based on the energy hub model. Using market elasticity as a basis, a price-based demand response model has been built. The model has been optimized to minimize daily operating cost of the multi-energy collaborative system. Using data from an actual situation, the model has been verified, and we have shown that the adoption of price-based demand response measures can significantly improve the economy of multi-energy collaborative systems. | Yuwei Cao Liying Wang Shigong Jiang Weihong Yang Ming Zeng Xiaopeng Guo | 2020 | Global Energy Interconnection2020，3，5： | 4 |
| 3 | Characteristics of SARS-CoV-2 Delta variant-infected individuals with intermittently positive retest viral RNA after discharge显示文摘SARS-CoV-2-infected patients will be discharged when viral ribonucleic acid(RNA)is consecutively negative at least twice over a 24-h interval.However,we have demonstrated that positive retest viral RNA,a particular clinical phenomenon initially observed in discharged SARS-CoV-2-infected patients in early 2020[1,2],is the occasional reoccurrence of Polymerase Chain Reaction(PCR)-detectable viral RNA but not reinfection[3]. | Lu Li Jingyan Tang Zhiwei Xie Qingxin Gan Guofang Tang Zhongwei Hu Huimin Zeng Jingrong Shi Jiaojiao Li Yan Li Changwen Ke Min Kang Dan Liang Huan Lu Yuwei Tong Xilong Deng Jinxin Liu Hongzhou Lu Fuxiang Wang Fengyu Hu Feng Li Nanshan Zhong Xiaoping Tang | 2022 | National Science Review2022，9，10： | 3 |
| 4 | The Chinese Teacher's Role in The Teaching of Culture Through Language显示文摘 | | 1997 | Teaching English in China1997，，30： | 1 |
| 5 | An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo显示文摘Background: Male germline stem cells(MGSCs) are a subpopulation of germ cells in the testis tissue. MGSCs are capable of differentiation into spermatozoa and thus are perfect targets for genomic manipulation to generate transgenic animals.Method: The present study was to optimize a protocol of production of transgenic mice through transduction of MGSCs in vivo using lentiviral-based vectors. The recombinant lentiviral vectors with either EF-1 or CMV promoter to drive the expression of enhanced green fluorescent protein(e GFP) transgene were injected into seminiferous tubules or inter-tubular space of 7-day-old and 28-day-old mouse testes. At 5 or 6 wk post-surgery, these pre-founders were mated with wild-type C57BL/6J female mice(1.5 to 2.0-month-old).Results: Sixty-seven percent of F1 generation and 55.56 % of F2 offspring were positive for eG FP transgene under the control of EF-1 promoter via PCR analysis. The transgenic pups were generated in an injection site-and age-independent manner. The expression of transgene was displayed in the progeny derived from lentiviral vector containing CMV promoter to drive transgene, but it was silenced or undetectable in the offspring derived from lentiviral vector with transgene under EF-1 promoter. The methylation level of g DNA in the promoter region of transgene was much higher in the samples derived lentiviral vectors with EF-1 promoter than that with CMV promoter,suggesting e GFP transgene was suppressed by DNA methylation in vivo.Conclusion: This research reported here an effective strategy for generation of transgenic mice through transduction of MGSCs in vivo using lentivirus vectors with specific promoters, and the transgenic offspring were obtained in an injection site-and age-independent manner. This protocol could be applied to other animal species, leading to advancement of animal transgenesis in agricultural and biomedical fields. | Jinzhou Qin Haixia Xu Pengfei Zhang Conghui Zhang Zhendong Zhu Rongfeng Qu Yuwei Qin Wenxian Zeng | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 1 |
| 6 | Two methods of chloral hydrate given drugs for pediatric calming effect observation显示文摘 | Liu Zeng Xiang Yuan Junjun Hu Yuwei | 2010 | Journal of practical medicine in China2010，，27： | 1 |
| 7 | Effects of the PARP inhibitor Niraparib on the radiosensitivity of human lung and cervical cancer cells显示文摘ObjectiveTo investigate the effect of Niraparib,a clinically approved PARP inhibitor on the radiosensitivity of human lung and cervical cancer cells,and preliminarily explore the underlying mechanism.MethodsThe human lung cancer cell line A549 and human cervical cancer cell line Siha were both treated with Niraparib for 1h,or X-ray irradiation with 4y,or Niraparib for 1h combined with X-ray irradiation with 4Gy.The effect was examined via measurements of cell proliferation by the cell counting kit-8 assay,and cell viability was detected by clone formation assays.Cell apoptosis and cell cycle distribution were investigated by flow cytometry.ResultsIn human lung and cervical cancer cell lines,Niraparib combined with radiation therapy significantly inhibited cell proliferation.The proportion of apoptotic cells in cell lines treated with Niraparib plus radiation(the combination group)was significantly higher compared with control,radiation,and Niraparib groups(P<0.05).Additionally,the proportion of A549 cells in the G_(2)/M phase was significantly increased in the combination group compared with the radiation group(P<0.05).ConclusionPARP inhibitor Niraparib increases the radiosensitivity of tumorcells,promotes their apoptosis,and induces cell cycle redistribution.The possible mechanism is associated with the inhibition of radiation-induced DNA damage repair. | Yue Feng Shiyi Pi Yuwei Li Yu Zhang Hongwei Zeng Xiangyu E Yang Zhang Jingping Yu | 2023 | Radiation Medicine and Protection2023，4，2： | 1 |
| 8 | Encouraging Active Viewing, Rather Than Passive Watching in the Audio-visual English Class显示文摘 | Yuwei Zeng | 2005 | Sino-US English Teaching2005，2，2： | 1 |
| 9 | Thirteen Dipterocarpoideae genomes provide insights into their evolution and borneol biosynthesis显示文摘Dipterocarpoideae,the largest subfamily of the Dipterocarpaceae,is a dominant component of Southeast Asian rainforests and is widely used as a source of wood,damar resin,medicine,and essential oil.However,many Dipterocarpoideae species are currently on the IUCN Red List owing to severe degradation of their habitats under global climate change and human disturbance.Genetic information regarding these taxa has only recently been reported with the sequencing of four Dipterocarp genomes,providing clues to the function and evolution of these species.Here,we report on 13 high-quality Dipterocarpoideae genome assemblies,ranging in size from 302.6 to 494.8 Mb and representing the five most species-rich genera in Dipterocarpoideae.Molecular dating analyses support the Western Gondwanaland origin of Dipterocarpaceae.Based on evolutionary analysis,we propose a three-step chromosome evolution scenario to describe the karyotypic evolution from an ancestor with six chromosomes to present-day species with 11 and 7 chromosomes.We discovered an expansion of genes encoding cellulose synthase(CesA),which is essential for cellulose biosynthesis and secondary cell-wall formation.We functionally identified five bornyl diphosphate synthase(BPPS)genes,which specifically catalyze the biosynthesis of borneol,a natural medicinal compound extracted from damar resin and oils,thus providing a basis for large-scale production of natural borneol in vitro. | Zunzhe Tian Peng Zeng Xiaoyun Lu Tinggan Zhou Yuwei Han Yingmei Peng Yunxue Xiao Botong Zhou Xue Liu Yongting Zhang Yang Yu Qiong Li Hang Zong Feining Zhang Huifeng Jiang Juan He Jing Cai | 2022 | Plant Communications2022，3，6： | 1 |
| 10 | Antioxidant-enriched autologous biogel promoted diabetic wound healing by remodeling inherent posttraumatic inflammatory patterning and restoring compromised microenvironment homeostasis显示文摘Successful wound healing depends on the reconstruction of proper tissue homeostasis,particularly in the posttraumatic inflammatory tissue microenvironment.Diabetes jeopardizes tissues’immune homeostasis in cutaneous wounds,causing persistent chronic inflammation and cytokine dysfunction.Previously,we developed an autologous regeneration factor(ARF)technology to extract the cytokine composite from autologous tissue to restore immune homeostasis and promote wound healing.However,treatment efficacy was significantly compromised in diabetic conditions.Therefore,we proposed that a combination of melatonin and ARF,which is beneficial for proper immune homeostasis reconstruction,could be an effective treatment for diabetic wounds.Our research showed that the utilization of melatonin-mediated ARF biogel(AM gel)promoted diabetic wound regeneration at a more rapid healing rate.RNA-Seq analysis showed that AM gel treatment could restore more favorable immune tissue homeostasis with unique inflammatory patterning as a result of the diminished intensity of acute and chronic inflammation.Currently,AM gel could be a novel and promising therapeutic strategy for diabetic wounds in clinical practice through favorable immune homeostatic reconstructions in the tissue microenvironment and proper posttraumatic inflammation patterning. | Yixi Yang Le Wang Yonglin Zhou Yijun He Shaozhang Lin Yuwei Zeng Yunhe Zhou Wei Li Zaopeng He Qi Zhao Lihao Chen Zijie Li Wenhao Wang Zhi-Yong Zhang | 2022 | Regenerative Biomaterials2022，9，1： | 0 |
| 11 | When and How to Adjust Non-Pharmacological Interventions Concurrent with Booster Vaccinations Against COVID-19—Guangdong,China,2022显示文摘Introduction:With the large-scale roll-out of the coronavirus disease 2019(COVID-19)booster vaccination effort(a vaccine dose given 6 months after completing primary vaccination)in China,we explore when and how China could lift non-pharmacological interventions(NPIs)against COVID-19 in 2022.Methods:Using a modified susceptible-infectiousrecovered(SIR)mathematical model,we projected the COVID-19 epidemic situation and required medical resources in Guangdong Province,China.Results:If the number of people entering from overseas recovers to 20%of the number in 2019,the epidemic in 2022 could be controlled at a low level by a containment(215 local cases)or suppression strategy(1,397 local cases).A mitigation strategy would lead to 21,722 local cases.A coexistence strategy would lead to a large epidemic with 6,850,083 local cases that would overwhelm Guangdong’s medical system.With 50%or 100%recovery of the 2019 level of travelers from overseas,the epidemic could also be controlled with containment or suppression,but enormous resources,including more hotel rooms for border quarantine,will be required.However,coexistence would lead to an uncontrollable epidemic with 12,922,032 local cases.Discussion:With booster vaccinations,the number of travelers from overseas could increase slightly in 2022,but a suppression strategy would need to be maintained to ensure a controllable epidemic. | Guanhao He Fangfang Zeng Jianpeng Xiao Jianguo Zhao Tao Liu Jianxiong Hu Sicong Zhang Ziqiang Lin Huaiping Zhu Dan Liu Min Kang Haojie Zhong Yan Li Limei Sun Yuwei Yang Zhixing Li Zuhua Rong Weilin Zeng Xing Li Zhihua Zhu Xiaofeng Liang Wenjun Ma | 2022 | China CDC weekly2022，4，10： | 0 |
| 12 | Unraveling the Effect of Cation Types on Electrochromic Properties of Titanium Dioxide Nanocrystals显示文摘Electrochromic(EC)devices have been regarded as promising candidates for energy-saving smart windows,next-generation displays,and wearable electronics.Monovalent ions such as H^(+)-and Li^(+)-based electrolytes are the benchmark insertion ions for EC devices but have serious limitations such as high cost,instability,and difficulty to handle.Seeking multivalent electrolytes is an effective alternative way to prepare high-performance EC devices;unfortunately,the related reports are currently limited to tungsten oxide EC materials.Herein,for the first time,we investigate the EC properties driven by different valence cationic(i.e.,Li^(+),Zn^(2+),and Al^(3+))electrolytes in the titanium dioxide system.It is found that the initial optical modulation ranges of TiO_(2)nanocrystal(NC)films in Li^(+),Zn^(2+),and Al^(3+)electrolytes are 76.8%,77.4%,and 77.3%,respectively.After 250 cycles,the optical contrast of these films in Zn^(2+)electrolyte decreased by only 2.3%,much lower than that in benchmark Li^(+)electrolyte of 10.1%and Al^(3+)electrolyte of 59.1%.Density functional theory calculation indicates that the potential barriers of Li^(+),Zn^(2+),and Al^(3+)in TiO_(2)are 0.59,0.55,and 0.74 eV,respectively,which makes TiO_(2)NCs show good EC properties in Zn^(2+)electrolytes.This work unravels the effect of different valence cations on the electrochromic properties of titanium dioxide NCs,which may provide some new directions for the development of excellent EC devices with long-term stability and durability. | Yi Liang Sheng Cao Yuwei Liu Lijuan He Xinxin Han Ruosheng Zeng Jialong Zhao Bingsuo Zou | 2022 | Energy Material Advances2022，，1： | 0 |
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