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| 1 | Enhanced efficiency of generating induced pluripotent stem (iPS) cells from human somatic cells by a combination of six transcription factors显示文摘 | Jing Liao Zhao Wu Ying Wang Lu Cheng Chun Cui Yuan Gao Taotao Chen Lingjun Rao Siye Chen Nannan Jia Huiming Dai Shunmei Xin Jiuhong Kang Gang Pei Lei Xiao | 2008 | Cell Research2008，18，5： | 61 |
| 2 | Immunohisto chemical Study on the Effects of Pyrethroids on Gluta-mate and γ-Aminobutyric Acid in Rat Central Nervous System显示文摘Objective\ In order to study the effects of pyrethroids on glutamate (Glu) and gamma aminobutyric acid (GABA) in rat central nervous system. Methods\ Male SD rats were treated with permethrin (7 mg/kg, i.p) or cypermethrin (70 mg/kg, i.p) once a day for 3 days. Glu and GABA immunoreactive cells and transmitters in CNS were studied by immunohistochemical method. Results\ The total number, percentage of positive area and integral optical density of glutamate immunoreactive cell in cerebral cortex, hippocampus decreased significantly in permethrin or cypermethrin, while GABA was enhanced. Cypermethrin is more tent than permethrin on either Glu or GABA. Conclusion\ Disturbance of Glu and GABA is likely to play a role in the development of pyrethroids excitatory neurotoxicity. | Wu Jianping, Lu Chun\+1, Wang Ying, et al. Wu Jianping, Lu Chun\+1, Wang Ying, et al. Institute of Toxicology, Nanjing Medical University, Nanjing\ 210029 Acta Universitatis Medicinalis Nanjing,19(6):450 | 1999 | The Journal of Biomedical Research1999，23，2： | 68 |
| 3 | Effect of hepatitis C virus infection on expression of several cancer-associated gene products in hepatocellular carcinoma显示文摘NTRODUCTIONOurpreviousstudiesbyseroepidemiological,molecularepidemiologicalandimmunopathologicalmethodshaverevealedthathepa... | YANG Jian Min, WANG Rong Quan, BU Bao Guo, ZHOU Zi Cheng, FANG Dian Chun and LUO Yuan Hui | 1999 | World Journal of Gastroenterology1999，5，1： | 42 |
| 4 | PM_(2.5) in an industrial district of Zhengzhou,China:Chemical composition and source apportionment显示文摘郑州是在中国的一个发展中的城市,那被微粒物质的高水平重重地弄脏。在这研究,好微粒物质(PM2.5 ) 在在 2010 的郑州的一个工业区域为他们的化学作文(可溶的离子,元素,元素的碳(EC ) 和器官的碳(OC )) 镇定、分析。分别地, PM2.5 的平均集中与 175 g/m3 的年度一般水准是 181, 122, 186 和 211 g/m3 春天,夏天,秋天和冬季了,远超过美国国家空气质量标准(15 g/m3 ) 的 PM2.5 规定。在郑州的 PM2.5 的主导的部件是第二等的离子(硫酸盐和硝酸盐) 和碳部分。可溶的离子,全部的碳和元素分别地贡献了 41% PM2.5 质量, 13% 和 3% 。土壤灰尘,第二等的喷雾器和煤燃烧,各贡献大约 26% 全部的 PM2.5 质量, 24% 和 23% ,是 PM2.5 的主要来源,根据积极矩阵因式分解分析的结果。生物资源烧的混合来源,油燃烧和焚化贡献了 13% PM2.5。从车辆和工业产生的好微粒物质分别地贡献了大约 10% PM2.5 和 4% 。 | Ningbo Geng Jia Wang Yifei Xu Wending Zhang Chun Chen Ruiqin Zhang | 2013 | Particuology2013，11，1： | 46 |
| 5 | Spatial and temporal variation of particulate matter and gaseous pollutants in 26 cities in China显示文摘O3and PM2.5were introduced into the newly revised air quality standard system in February 2012, representing a milestone in the history of air pollution control, and China's urban air quality will be evaluated using six factors(SO2, NO2, O3, CO, PM2.5and PM10) from the beginning of 2013. To achieve the new air quality standard, it is extremely important to have a primary understanding of the current pollution status in various cities. The spatial and temporal variations of the air pollutants were investigated in 26 pilot cities in China from August 2011 to February 2012, just before the new standard was executed. Hourly averaged SO2, NO2and PM10were observed in 26 cities, and the pollutants O3, CO and PM2.5were measured in 15 of the 26 cities. The concentrations of SO2and CO were much higher in the cities in north China than those in the south. As for O3and NO2, however, there was no significant diference between northern and southern cities. Fine particles were found to account for a large proportion of airborne particles, with the ratio of PM2.5to PM10ranging from 55% to 77%. The concentrations of PM2.5(57.5 μg/m3) and PM10(91.2 μg/m3) were much higher than the values(PM2.5: 11.2 μg/m3; PM10 : 35.6 μg/m3) recommended by the World Health Organization. The attainment of the new urban air quality standard in the investigated cities is decreased by 20% in comparison with the older standard without considering O3, CO and PM2.5, suggesting a great challenge in urban air quality improvement, and more eforts will to be taken to control air pollution in China. | Fahe Chai Jian Gao Zhenxing Chen Shulan Wang Yuechong Zhang Jingqiao Zhang Hefeng Zhang Yaru Yun Chun Ren | 2014 | Journal of Environmental Sciences2014，26，1： | 36 |
| 6 | Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems显示文摘The CRISPR/Cas system has been extensively applied to make precise genetic modifications in various organisms. Despite its importance and widespread use, large-scale mutation screening remains time-consuming, labour-intensive and costly. Here, we developed Hi-TOM(available at http://gffzz103f3ce5d9b14419hcuk596uk09uq69wo.ffgz.tsg.suse.edu.cn/hi-tom/), an online tool to track the mutations with precise percentage for multiple samples and multiple target sites. We also described a corresponding next-generation sequencing(NGS) library construction strategy by fixing the bridge sequences and barcoding primers. Analysis of the samples from rice, hexaploid wheat and human cells reveals that the Hi-TOM tool has high reliability and sensitivity in tracking various mutations, especially complex chimeric mutations frequently induced by genome editing. Hi-TOM does not require special design of barcode primers,cumbersome parameter configuration or additional data analysis. Thus, the streamlined NGS library construction and comprehensive result output make Hi-TOM particularly suitable for high-throughput identification of all types of mutations induced by CRISPR/Cas systems. | Qing Liu Chun Wang Xiaozhen Jiao Huawei Zhang Lili Song Yanxin Li Caixia Gao Kejian Wang | 2019 | Science China(Life Sciences)2019，62，1： | 37 |
| 7 | Normoalbuminuric diabetic kidney disease显示文摘 | Chao Chen Chang Wang Chun Hu Yachun Han Li Zhao Xuejing Zhu Li Xiao Lin Sun | 2017 | Frontiers of Medicine2017，11，3： | 38 |
| 8 | Preliminary engineering application of microseismic monitoring technique to rockburst prediction in tunneling of Jinping Ⅱ project显示文摘Monitoring and prediction of rockburst remain to be worldwide challenges in geotechnical engineering.In hydropower,transportation and other engineering fields in China,more deep,long and large tunnels have been under construction in recent years and underground caverns are more evidently featured by 'long,large,deep and in group',which bring in many problems associated with rock mechanics problems at great depth,especially rockburst.Rockbursts lead to damages to not only underground structures and equipments but also personnel safety.It has been a major technical bottleneck in future deep underground engineering in China.In this paper,compared with earthquake prediction,the feasibility in principle of monitoring and prediction of rockbursts is discussed,considering the source zones,development cycle and scale.The authors think the feasibility of rockburst prediction can be understood in three aspects:(1) the heterogeneity of rock is the main reason for the existence of rockburst precursors;(2) deformation localization is the intrinsic cause of rockburst;and(3) the interaction between target rock mass and its surrounding rock mass is the external cause of rockburst.As an engineering practice,the application of microseismic monitoring techniques during tunnel construction of Jinping II Hydropower Station was reported.It is found that precursory microcracking exists prior to most rockbursts,which could be captured by the microseismic monitoring system.The stress concentration is evident near structural discontinuities(such as faults or joints),which shall be the focus of rockburst monitoring.It is concluded that,by integrating the microseismic monitoring and the rock failure process simulation,the feasibility of rockburst prediction is expected to be enhanced. | Chun'an Tang Jimin Wang Jingjian Zhang | 2010 | Journal of Rock Mechanics and Geotechnical Engineering2010，2，3： | 32 |
| 9 | Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors显示文摘在 enucleated 鸡蛋的体的房间的 Reprogramming 做了多丽,绵羊,在 1996 的第一个成功地克隆的哺乳动物。然而,绵羊的机制体的房间 reprogramming 还没被探讨了。而且,绵羊胚胎的茎(ES ) 细胞仍然不是可得到的,它限制精确修改基因的绵羊的产生。在这研究,我们报导体的房间能是的那只绵羊直接 reprogrammed 到导致的 pluripotent 用定义因素(Oct4, Sox2, c-Myc, Klf4, Nanog, Lin28, SV40 大 T 和 hTERT ) 的茎(iPS ) 房间。我们的观察显示从绵羊的体的细胞比从另外的种,在 iPS,细胞被报导了的体的细胞对改编更困难。我们证明绵羊 iPS 房间表示 ES 房间标记,包括碱的磷酸酶, Oct4, Nanog, Sox2, Rex1,阶段特定的胚胎的 antigen-1, TRA-1-60, TRA-1-81 和 E-cadherin。绵羊 iPS 房间展出了正常 karyotypes 并且能在 vitro 并且在 teratomas 区分进所有三细菌层。我们的学习可以帮助在绵羊揭示体的房间 reprogramming 的机制并且提供一个平台为绵羊 ES 房间探索文化条件。而且,绵羊 iPS 房间可以直接被用来产生精确修改基因的绵羊。 | Lei Bao Lixiazi He Jijun Chen Zhao Wu Jing Liao Lmgdun Rao Jiangtao Ren Hui Li Hui Zhu Lei Qian Yijun Gu Huimin Dai Xun Xu Jinqiu Zhou Wen Wang Chun Cui Lei Xiao | 2011 | Cell Research2011，21，4： | 32 |
| 10 | Clinical efficacy and safety of moxifloxacin versus levofloxacin plus metronidazole for community-acquired pneumonia with aspiration factors显示文摘 | Sun Tieying Sun Li Wang Rongmei Ren Xiaoping Sui Dong-jiang Pu Chun Ren Yajuan Liu Ying Yang Zhuo Li Fengzhi | 2014 | Chinese Medical Journal2014，，7： | 34 |
| 11 | Expanding the Range of CRISPR/Cas9 Genome Editing in Rice显示文摘 | Xixun Hu Chun Wang Yaping FU Qing Liu Xiaozhen Jiao Kejian Wang | 2016 | Molecular Plant2016，9，6： | 31 |
| 12 | The Association of Maternal Body Composition and Dietary Intake with the Risk of Gestational Diabetes Mellitus during the Second Trimester in a Cohort of Chinese Pregnant Women显示文摘Objective To investigate the association of maternal body composition and dietary intake with the risk of gestational diabetes mellitus(GDM). Methods A total 154 GDM subjects and 981 controls were enrolled in a prospective cohort study in 11 hospitals from May 20, 2012 to December 31, 2013. Bioelectrical impedance analysis and dietary surveys were used to determine body composition and to evaluate the intake of nutrients in subjects at 21-24 weeks' gestation(WG). Logistic regression analysis was applied to explore the relationships of maternal body composition and dietary intake with the risk of GDM morbidity. Results Age, pre-pregnant body weight(BW), and body mass index(BMI) were associated with increased risk of GDM. Fat mass(FM), fat mass percentage(FMP), extracellular water(ECW), BMI, BW, energy, protein, fat, and carbohydrates at 21-24 WG were associated with an increased risk of GDM. In contrast, fat free mass(FFM), muscular mass(MM), and intracellular water(ICW) were associated with a decreased risk of GDM. Conclusion Maternal body composition and dietary intake during the second trimester of pregnancy were associated with the risk of GDM morbidity. | Xu Qing Gao Zhi Ying Li Li Ming Wang Lu Zhang Qian Teng Yue Zhao Xia Ge Sheng Jing Hong Jiang Yang Yong Tao Liu Xiao Jun Lyu Chun Jian Mao Lun Yu Xiao Ming Liu Ying Hua Kong Ai Jing Yang Xue Yan Liu Zhao Zhang Yong Wang Jin Zhang Xin Sheng Xue Chang Yong Lu Yan Ping | 2016 | Biomedical and Environmental Sciences2016，29，1： | 29 |
| 13 | Chinese Multidisciplinary Expert Consensus on the Diagnosis and Treatment of Hyperuricemia and Related Diseases显示文摘 | Zou, He-Jian Wu, Hu-Sheng Zhou, Jing-Guo Zeng, Xue-Jun Dai, Lie Wu, Hua-Xiang Zhu, Xiao-Xia Mei, Chang-Lin Hao, Chuan-Ming Chen, Nan Liu, Bi-Cheng Chen, Jiang-Hua Yang, Li Nie, Jing Yu, Chen Peng, Ai Yu, Sheng-Qiang Li, Lin Ge, Jun-Bo Huo, Yong Zhang, Shu-Yang Chen, Yun-Dai Dong, Yu-Gang Liang, Chun Dai, Yu-Xiang Gao, Xin Li, Chang-Gui Zhao, Jia-Jun Chen, Hai-Bing Cheng, Zhi-Feng Lin, Huan-Dong Guan, Yang-Tai Wang, Kai Luo, Ben-Yan Dai, Ruo-Lian Jiang, Quan Xue, Luan Liang, Chao-Chao Chen, Ming Fan, Song | 2017 | Chinese Medical Journal2017，，20： | 30 |
| 14 | Chemistry and pharmacology of Siraitia grosvenorii: A review显示文摘Siraitia grosvenorii is a perennial herb endemic to Guangxi province of China. Its fruit, commonly known as Luo hanguo, and has been used for hundreds of years as a natural sweetener and as a traditional medicine for the treatment of pharyngitis, pharyngeal pain, as well as an anti-tussive remedy in China. Based on ninety-three literary sources, this review summarized the advances in chemistry, biological effects, and toxicity research of S. grosvenorii during the past 30 years. Several different classes of compounds have been isolated or detected from various parts of S. grosvenorii, mainly triterpenoids, flavonoids, polysaccharides, amino acids, and essential oils. Various types of extracts or individual compounds derived from this species exhibited a wide array of biological effects e.g. anti-tussive, phlegm-relieving, anti-oxidant, immunomodulatory, liver-protecting, glucose-lowering, and anti-microbial. The existing research has shown that extracts and individual compounds from S. grosvenorii are basically non-toxic. Finally, some suggestions for further research on specific chemical and pharmacological properties of S. grosvenorii are proposed in this review. | LI Chun LIN Li-Mei SUI Feng WANG Zhi-Min HUO Hai-Ru DAI Li JIANG Ting-Liang | 2014 | Chinese Journal of Natural Medicines2014，12，2： | 28 |
| 15 | Regulating effects of arsenic trioxide on cell death pathways and inflammatory reactions of pancreatic acinar cells in rats显示文摘背景煽动性的 cytokines 在尖锐胰腺炎的发展起一个关键作用,这被接受,那么堵住煽动性的反应的开始可以减轻尖锐胰腺炎的病理学的变化。我们在 vitro 在胰腺的 acinar 房间的 apoptosis 和 oncosis 上学习了砷三氧化物(As2O3 ) 的规章的效果,在尖锐 pancreatitis.Methods 上的 vivo 和它的治疗学的效果,胰腺的 acinar 房间被 collagenase 消化方法孤立。孤立的胰腺的 acinar 房间的 Apoptosis 和 oncosis 与 Hoechst 33258+PI 或 Annexin V+PI 被检测两倍荧光灯的染色。淀粉酶并且喂奶脱氢酶版本被测量。尖锐胰腺炎被 caerulein 的 intraperitoneal 注射在 Wistar 老鼠导致,并且 apoptosis 与把方法标记的终端 dUTP 刻痕结束被检测。肿瘤 necorsis 因素(TNF-) mRNA, myeloperoxidase,原子 factor-B 并且胰腺的损坏的组织学的分级是在那里的 measured.Results 是增加的 apoptosis 但是在有 As2O3 的处理以后的胰腺的 acinar 房间的减少的 oncosis。层次喂奶脱氢酶和淀粉酶版本显著地在在对待的 As2O3 的对待的 group.Myeloperoxidase 内容,TNF- mRNA 水平,原子 factor-B 激活和病理学的分数组织的 As2O3 被减少比在未经治疗的 group.Conclusions As2O3 能导致 apoptosis 和胰腺的 acinar 的还原剂 oncosis 的显著地低房间,因此导致 acinar 房间的 endocellular 酶的减少的版本,减少了煽动性的房间渗入并且减少 inflamma | XUE Dong-bo ZHANG Wei-hui YUN Xiao-guang SONG Chun ZHENG Biao SHI Xing-ye WANG Hai-yang | 2007 | Chinese Medical Journal2007，，8： | 26 |
| 16 | Hypoxic preconditioning attenuates hypoxia/reoxygenation-induced apoptosis in mesenchymal stem cells显示文摘目的: 间充质的干细胞(MSC ) 是心脏的代替治疗的一个有希望的候选人。然而,因为差的血供给, ischemia-reperfusion,和煽动性的因素,移植 MSC 的多数乐意地在移植以后被失去。我们试图在 MSC.Methods 的 hypoxia/reoxygenation-induced apoptosis 上学习组织缺氧 preconditioning ( HPC )的效果: MSC 的三代被划分成 6 个组包括正常的组, hypoxia-reoxygenation ( H/R )组, cyclosporine A ( CsA ),和 HPC 10 min , 20 min ,并且 30 min 组织。 apoptotic 索引,房间生存能力, mitochondrial 膜电位, Bcl-2 和 bax 的易位,细胞外的调整激酶(英皇家空军之阶级最低之兵), Akt ,组织缺氧可诱导的因素 1-alpha ,并且脉管的内皮生长因素( VEGF )在 H/R treatment.Results 以后被测试: HPC 减少了 apoptotic 索引并且增加了 H/R.Moreover 导致的生存能力, HPC 显著地稳定了 mitochondrial 膜电位在调整 Bcl-2 和 VEGF 表达式上面,并且增加了英皇家空军之阶级最低之兵和 Akt.As 的磷酸化积极控制, CsA 有象 HPC 的一样的函数,除了支持英皇家空军之阶级最低之兵和 Akt 磷酸化并且在调整 VEGF.Conclusion 上面: HPC 对 H/R 经由稳定 mitochondrial 膜电位导致的 MSC apoptosis 有保护的效果在调整 Bcl-2 和 VEGF 上面,并且支持英皇家空军之阶级最低之兵和 Akt phosphorylation.HPC 为新奇干细胞protective 策略的开发有含意。 | Jian-an WANG Tie-long CHEN Jun JIANG Heng SHI Chun GUI Rong-hua LUO Xiao-jie XIE Mei-xiang XIANG Xing ZHANG | 2008 | Acta Pharmacologica Sinica2008，29，1： | 25 |
| 17 | Changes of activated circulating endothelial cells and survivin in patients with non-small cell lung cancer after antiangiogenesis therapy显示文摘背景尽管 antiangiogenesis 治疗与它的公认的功效和细微不利效果在反肿瘤的治疗起一个重要作用,没有未来的临床的试用为预言 antiangiogenic therapy.This 学习的功效被承担在预言非小的 cel 的 therapy.Methods 病人的功效在 anti-angiogenesis 治疗和他们的意义以后调查激活的传播 endothelial 房间( aCECs )和 survMn | WANG Jing HUANG Chun WEI Xi-yin QI Da-liang GONG Li-qun MU Hai-yu YAO Qiang LI Kai | 2008 | Chinese Medical Journal2008，，22： | 24 |
| 18 | A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice显示文摘Generating mutants bearing multiple gene modifications is essential for determining the functions of gene family members with redundant functions,or for analyzing epistatic relationships in genetic pathways.Using conventional methods,mutants with multiple gene mutations are generated by several rounds of intercrossing plants carrying a single mutation and identification of the offspring.This process is both time- | Chun Wang Lan Shen Yaping Fu Changjie Yan Kejian Wang | 2015 | Journal of Genetics and Genomics2015，42，12： | 22 |
| 19 | Alteration of p53 and p21 during hepatocarcinogenesis in tree shrews显示文摘AIM: To investigate p53 mutation and p21 expression in hepatocarcinogenesis induced by hepatitis B virus (HBV) and aflatoxin B1 (AFB1) in tree shrews, and to reveal the role of these genes in hepatocarcinogenesis.METHODS: Tree shrews were divided into four groups:group A, those infected with HBV and fed with AFB1 (n = 39);group B, those infected with HBV alone (n = 28); group C,those fed with AFB1 alone (n = 29); and group D, normal controls (n = 20). The tree shrews underwent liver biopsies once every 15 wk. Expression of p53 and p21 proteins and genes in the biopsies and tumor tissues of the experimental tree shrews was detected, respectively, by immunohistochemistry,and by Southem blotting and reverse transcription-polymerase chain reaction and sequencing.RESULTS: The incidence of hepatocellular carcinomas (HCC) was higher in group A (66.7%) than that in group B (3.57%) and C (30%). The time of HCC occurrence was also earlier in group A than that in group C (120.0±16.6 wk vs 153.3±5.8 wk, respectively, P<0.01). p53 protein was not detected by immunohistochemistry in all groups before the 75^th wk of the experiment. At the 105^th wk, the positive rates fo p53 were 78.6%, 60% and 71.4% in groups A, B and C, respectively, which were significantly higher than that in group D (10%) (all P<0.05). An abnormal band of p53 gene was observed in groups A and C. The mutation points of p53gene in tree shrews with HCC were at codons 275, 78 and 13. The nucleotide sequence and amino acid sequence of tree shrew's wild-type p53 showed 91.7% and 93.4% homologies with those of human p53,respectively. The immunopositivity for p21 was found before HCC development. The incidence of HCC was significantly higher in tree shrews that were positive for p21 than those negative for p21 (80.0% vs 11.0%, P<0.001).The incidence of HCC in p21 positive animals in group A was significantly higher than those positive for p21 in group C (P | Jian-Jia Su Yuan Li Ke-Chen Ban Liu-Liang Qin Chun Yang Chao Ou Xiao-Xian Duan Hui-Yun Wang Rui-Qi Yang Young-Lk Lee | 2004 | World Journal of Gastroenterology2004，10，24： | 21 |
| 20 | Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp × common carp显示文摘This study investigated the gynogenetic cytobiological behavior of the third gynogenetic generation (G3), which was generated from the diploid eggs produced by the second gynogenetic generation (G2) of red crucian carp × common carp, and determined the chromosomal numbers of G3, G2×scatter scale carp and G2×allotetraploid hybrids of red crucian carp × common carp. The results showed that the diploid eggs of G2 with 100 chromosomes, activated by UV-irradiated sperm from scatter scale carp and without the treatment for doubling the chromosomes, could develop into G3 with 100 chromosomes. Similar to the first and second gynogenetic generations (G1 and G2), G3 was also diploid (2n=100) and presented the hybrid traits. The triploids (3n=150) and tetraploids (4n=200) were produced by crossing G2 with scatter scale carp and crossing G2 with allotetraploids, respectively. The extrusion of the second polar body in the eggs of G2 ruled out the possibility that the retention of the second polar body led to the formation of the diploid eggs. In addition, we discussed the mechanism of the formation of the diploid eggs generated by G2. The establishment of the diploid gynogenesis clonal line (G1, G2 and G3) provided the evidence that the diploid eggs were able to develop into a new diploid hybrid clonal line by gynogenesis. By producing the diploid eggs as a unique reproductive way, the diploid gyno- genetic progeny of allotetraploid hybrids of red crucian carp × common carp had important signifi- cances in both biological evolution and production application. | LIU ShaoJun, DUAN Wei, TAO Min, ZHANG Chun, SUN YuanDong, SHEN JiaMin, WANG Jing, LUO KaiKun & LIU Yun Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China | 2007 | Science China(Life Sciences)2007，50，2： | 21 |
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