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| 1 | Advantages of white LED lamps and new detector technology in photometry显示文摘Light emitting diode(LED)lighting is becoming more and more popular,as incandescent lamps are being phased out globally.LEDs have several advantages over incandescent lamps,including energy efficiency,robustness,long lifetime,and good temporal stability.The three latter features make LEDs attractive candidates as new photometric standards.Because the spectra of white LEDs are limited to the visible wavelength range,a novel method for the realization of photometric units based on the predictable quantum efficient detector(PQED)can be utilized.The method eliminates the need of photometric filters that are traditionally used in photometry,and instead relies on carrying out the photometric weighting numerically based on the measured relative spectrum of the source.The PQED-based realization simplifies the traceability chain of photometric measurements significantly as compared with the traditional filter-based method.The measured illuminance values of a white LED deviate by only 0.03%when determined by the new and the traditional methods.The new PQED method has significantly lower expanded uncertainty of 0.26%(k=52)as compared with that of the traditional filter-based method of 0.42%(k=52).Furthermore,when filtered photometers that measure LED lighting are calibrated using LED lamps as calibration sources instead of incandescent lamps,a significant decrease in the uncertainty related to the spectral mismatch correction can be obtained.The maximum spectral mismatch errors of LED measurements decreased on average by a factor of 3 when switching from an incandescent lamp to an LED calibration source. | Tomi Pulli Timo Donsberg Tuomas Poikonen Farshid Manoocheri Petri Karha Erkki Ikonen | 2015 | Light(Science & Applications)2015，4，1： | 8 |
| 2 | 基于磁珠和时间分辨荧光免疫分析的微囊藻毒素LR单链抗体筛选与鉴定显示文摘【目的】从鼠源合成噬菌体抗体库中,快速分离获得微囊藻毒素LR单链抗体。【方法】采用磁珠筛选和负筛选方法,设计两轮微囊藻毒素LR单链抗体的筛选方案。用噬菌体产出投入比和多克隆噬菌体免疫分析,对每轮筛选后微囊藻毒素LR特异性噬菌体的富集效果进行鉴定。再将第2轮筛选获得的次级库提取单链抗体基因,转入可溶性表达载体;诱导表达后,采用时间分辨荧光免疫法,对单个噬菌体克隆鉴定并测序。【结果】两轮筛选的噬菌体产出投入比分别为4.8×10-8和2.88×10-6。第2轮筛选后的次级库,经多克隆噬菌体免疫分析得到的荧光信噪比,比原始抗体库提高了22.8倍。最终鉴定得到5株不同的阳性噬菌体克隆,其中最佳的克隆对微囊藻毒素LR的检测灵敏度(IC10)为13 ng.mL-1,抑制中浓度(IC50)为435 ng.mL-1,线性范围在31—5 952 ng.mL-1。【结论】本研究筛选到的单链抗体,有潜力应用于微囊藻毒素LR的免疫学检测或免疫亲和柱的制备,同时也探索了一种高效的微囊藻毒素LR单链抗体的库筛选、鉴定方法。 | 刘媛 Huovinen Tuomas 刘贤金 梁颖 张存政 谢雅晶 贺江 王耘 张霄 | 2012 | 中国农业科学2012，45，2： | 8 |
| 3 | 时间分辨荧光免疫分析方法检测烟草中菌核净残留显示文摘建立了简便、灵敏地检测烟草中菌核净残留的时间分辨荧光免疫分析方法(TRFIA),及配套的无净化的快速前处理技术。采用镧系元素螯合物Eu-N1标记亲和纯化后的羊抗兔IgG示踪抗体。采用间接竞争TRFIA法建立了菌核净标准抑制曲线,方法的检出限I10为2.0μg/L;抑制终浓度I50为32.00μg/L;检测线性范围为4~128μg/L。考察了丙酮提取后33%,10%和1%的烟草基质对菌核净-TRFIA分析方法的影响,表明在1%烟叶基质条件下,建立的菌核净的抑制曲线的线性范围与标准抑制曲线趋于平行,确定烟草样品的前处理稀释倍数为100倍,计算得到基质影响因子(Im)为17.1。对烟叶样品中添加1,7和24mg/L的菌核净标样,连续3d的添加回收实验表明,方法的回收率为73%~128%,相对标准标准偏差(RSD)在4.3%~13.2%之间;烟草中菌核净的实际最低检出限为1mg/L。本方法可望用于烟草中菌核净残留的快速筛选检测。 | 刘媛 刘蓉蓉 刘贤金 方敦煌 宋春满 Huovinen Tuomas Vehniinen Markus Lvgren Timo | 2012 | 分析化学2012，40，7： | 6 |
| 4 | Cellulose structure and lignin distribution in normal and compression wood of the Maidenhair tree(Ginkgo biloba L.)显示文摘We studied in detail the mean micro fibril angle and the width of cellulose crystals from the pith to the bark of a 15-year-old Maidenhair tree(Ginkgo biloba L.). The orientation of cellulose micro fibrils with respect to the cell axis and the width and length of cellulose crystallites were determined using Xray diffraction. Raman microscopy was used to compare the lignin distribution in the cell wall of normal/opposite and compression wood, which was found near the pith. Ginkgo biloba showed a relatively large mean micro fibril angle,varying between 19° and 39° in the S2 layer, and the average width of cellulose crystallites was 3.1–3.2 nm. Mild compression wood without any intercellular spaces or helical cavities was observed near the pith. Slit-like bordered pit openings and a heavily lignified S2 L layer con firmed the presence of compression wood. Ginkgo biloba showed typical features present in the juvenile wood of conifers. The micro fibril angle remained large over the 14 annual rings. The entire stem disc,with a diameter of 18 cm, was considered to consist of juvenile wood. The properties of juvenile and compression wood as well as the cellulose orientation and crystalline width indicate that the wood formation of G. biloba is similar to that of modern conifers. | Seppo Andersson Yurong Wang Raili P?nni Tuomas H?nninen Marko Mononen Haiqing Ren Ritva Serimaa Pekka Saranp?? | 2015 | Journal of Integrative Plant Biology2015，57，4： | 5 |
| 5 | Synchrotron radiation investigations of the Sr_2MgSi_2O_7:Eu^(2+),R^(3+) persistent luminescence materials显示文摘The electronic and defect energy level structure of polycrystalline Sr2MgSi2O7:Eu2+,R3+ persistent luminescence materials were studied with thermoluminescence and different synchrotron radiation spectroscopies (UV-VUV emission and excitation, X-ray absorption near-edge spectroscopy (XANES) and extended X-ray absorption fine structure (EXAFS)). Special attention was paid on the effect of the R3+ co-dopants on the persistent luminescence properties of the materials. Theoretical calculations using the density functional theory (DFT) were carried out simultaneously with the experimental work. The experimental band gap energy (Eg) value of ca. 7.1 eV agreed very well with the DFT value of 6.7 eV. The variation of the Eg value was attempted to relate with the trap structure as well as with the different properties of the R3+ co-dopants. The trap level energy distribution depended strongly on the R3+ co-dopant except for the shallowest trap energy above the room temperature remaining practically the same, however. The different processes in the mechanism of persistent luminescence from Sr2MgSi2O7:Eu2+,R3+ were assembled and their contributions discussed. | Tuomas Aitasalo Jukka Hassinen Jorma Hls Taneli Laamanen Mika Lastusaari Marja Malkamki Janne Niittykoski Pavel Novák | 2009 | Journal of Rare Earths2009，27，4： | 5 |
| 6 | 冷战和环境:芬兰在波罗的海地区国际环境政治方面的角色显示文摘1974年在赫尔辛基签署的关于《波罗的海区域海洋环境保护公约》可能是在波罗的海地区最重要的环境保护协议。本篇文章是首次运用原始档案资料第一次探究本公约的历史,也称之为《赫尔辛基公约》。这些原始资料主要出自芬兰外交部的档案。我们从国际政治角度研究了《赫尔辛基公约》整个签署的过程中芬兰的作用。本研究主要集中在芬兰、瑞典和苏联从20世纪60年代末到1974年的国家政党。我们表明,冷战政治从若干方面影响《赫尔辛基公约》的谈判和内容。我们也认为苏联利用新出现的国际环境问题作为一种新的强权政治的工具。 | Tuomas Rsnen Simo Laakkonen 翟伟康 | 2007 | AMBIO-人类环境杂志2007，36，2： | 4 |
| 7 | Converting lateral scanning into axial focusing to speed up three-dimensional microscopy显示文摘In optical microscopy,the slow axial scanning rate of the objective or the sample has traditionally limited the speed of volumetric imaging.Recently,by conjugating either a movable mirror to the image plane in a remote-focusing geometry or an electrically tuneable lens(ETL)to the back focal plane,rapid axial scanning has been achieved.However,mechanical actuation of a mirror limits the axial scanning rate(usually only 10–100 Hz for piezoelectric or voice coil-based actuators),while ETLs introduce spherical and higher-order aberrations that prevent high-resolution imaging.In an effort to overcome these limitations,we introduce a novel optical design that transforms a lateral-scan motion into a spherical aberration-free axial scan that can be used for high-resolution imaging.Using a galvanometric mirror,we scan a laser beam laterally in a remote-focusing arm,which is then back-reflected from different heights of a mirror in the image space.We characterize the optical performance of this remote-focusing technique and use it to accelerate axially swept light-sheet microscopy by an order of magnitude,allowing the quantification of rapid vesicular dynamics in three dimensions.We also demonstrate resonant remote focusing at 12 kHz with a two-photon raster-scanning microscope,which allows rapid imaging of brain tissues and zebrafish cardiac dynamics with diffraction-limited resolution. | Tonmoy Chakraborty Bingying Chen Stephan Daetwyler Bo-Jui Chang Oliver Vanderpoorten Etai Sapoznik Clemens F.Kaminski Tuomas P.J.Knowles Kevin M.Dean Reto Fiolka | 2020 | Light(Science & Applications)2020，9，1： | 4 |
| 8 | Additive QTLs on three chromosomes control flowering time in woodland strawberry(Fragaria vesca L.)显示文摘Flowering time is an important trait that affects survival,reproduction and yield in both wild and cultivated plants.Therefore,many studies have focused on the identification of flowering time quantitative trait locus(QTLs)in different crops,and molecular control of this trait has been extensively investigated in model species.Here we report the mapping of QTLs for flowering time and vegetative traits in a large woodland strawberry mapping population that was phenotyped both under field conditions and in a greenhouse after flower induction in the field.The greenhouse experiment revealed additive QTLs in three linkage groups(LG),two on both LG4 and LG7,and one on LG6 that explain about half of the flowering time variance in the population.Three of the QTLs were newly identified in this study,and one co-localized with the previously characterized FvTFL1 gene.An additional strong QTL corresponding to previously mapped PFRU was detected in both field and greenhouse experiments indicating that gene(s)in this locus can control the timing of flowering in different environments in addition to the duration of flowering and axillary bud differentiation to runners and branch crowns.Several putative flowering time genes were identified in these QTL regions that await functional validation.Our results indicate that a few major QTLs may control flowering time and axillary bud differentiation in strawberries.We suggest that the identification of causal genes in the diploid strawberry may enable fine tuning of flowering time and vegetative growth in the closely related octoploid cultivated strawberry. | Samia Samad Takeshi Kurokura Elli Koskela Tuomas Toivainen Vipul Patel Katriina Mouhu Daniel James Sargent Timo Hytönen | 2017 | Horticulture Research2017，4，1： | 3 |
| 9 | Early Repolarization: Electrocardiographic Phenotypes Associated With Favorable Long-Term Outcome显示文摘 | Jani T. Tikkanen M. Juhani Junttila Olli Anttonen Aapo L. Aro Samuli Luttinen Tuomas Kerola Solomon J. Sager Harri A. Rissanen Robert J. Myerburg Antti Reunanen Heikki V. Huikuri | 2011 | Circulation2011，，23： | 3 |
| 10 | 服用抗氧化剂降低老年人血清脂质过氧化物——随机双盲临床试验显示文摘给某老年人护理院的45位老人服用β-胡萝卜素,维生素B_6,维生素C,维生素E,锌和硒,或者相应的安慰剂作为日常食物的补充,为期一年。开始时,这些老年人的血清脂质过氧化物水平(2.7±0.7μmol/L)高于作为对照组的年轻健康成年人(2.3±0.6μmol/L),P<0.01。服药三个月后,老年人的血清脂质过氧化物已降至2.2±0.6μmol/L,并且保持这一低水平直到研究结束,而服安慰剂者血清脂质过氧化物无变化。 血清脂质过氧化物与全血硒浓度呈显著负相关(r=-0.428,P<0.01),但只有在血硒高于200μg/L时才可见血清脂质过氧化物的降低。血清α-维生素E浓度也与血清脂质过氧化物呈负相关,但相关程度(r=-0.273,P<0.76)低于与血硒间的相关。 从生物化学角度看,25%的老年人可观察到维生素B_6缺乏;每天补充2mg维生素B_6彻底治愈了全部的病例。服药组经几项心理学检验有轻微改善,而服用安慰剂的对照组无变化或者恶化。护士报告在服药组中见有临床改善,未见有毒性副作用。老年人服用抗氧化剂从生物化学和临床上都是有益的。 | Matti Tolonen Seppo Sarna Tuomas Westermarck Markku Halme Ulla-Riitta Nordberg Mirja Keinonen Jaap Schrijver Seppo E.J.Tuominen 杨旭冬 | 1989 | 营养学报1989，11，1： | 3 |
| 11 | 新与旧的结合 水舍——南外滩精品酒店显示文摘正如郭锡恩曾说的'一座城市成熟时,便会开始寻找老的东西,就像人一样。''水舍'的设计即是将历史与现在的结合,让客人在旧与新的融合中体验一种时空穿梭的时代感。 | 郭锡恩 胡如珊 Debby Haepers 蔡春燕 马克思 Jane Wang Derryck Menere Tuomas Uusheimo | 2011 | 中国建筑装饰装修2011，，2： | 3 |
| 12 | Modelling inflation in China—A regional perspective显示文摘 | Aaron Mehrotra Tuomas Peltonen Alvaro Santos Rivera | 2009 | China Economic Review2009，，2： | 2 |
| 13 | Dead standing pine trees in a boreal forest landscape in the Kalevala National Park,northern Fennoscandia: amount,population characteristics and spatial pattern显示文摘Background: After their death, Scots pine trees can remain standing for decades and sometimes up to 200 years,forming long-lasting and ecologically important structures in boreal forest landscapes. Standing dead pines decay very slowly and with time develop into ‘kelo' trees, which are characterized by hard wood with silvery-colored appearance. These kelo trees represent an ecologically important, long lasting and visually striking element of the structure of natural pine-dominated forests in boreal Fennoscandia that is nowadays virtually absent from managed forest landscapes.Methods: We examined and mapped the amount, structural features, site characteristics and spatial distribution of dead standing pine trees over a ten hectare area in an unmanaged boreal forest landscape in the Kalevala National Park in Russian Viena Karelia.Results: The mean basal area of dead standing pine trees in the forested part of the landscape was 1.7 m^2?ha^(-1) and the estimated volume 12.7 m^3?ha^(-1). From the total number of standing dead pine trees 65% were kelo trees, with a basal area of 1.1 m^2?ha^(-1) and volume of 8.0 m^3?ha^(-1), the remainder consisting of standing dead pines along the continuum between a recently dead tree and a kelo tree. Overall, standing dead pines were distributed throughout the study area, but there was a tendency towards spatial clustering up to <100 m distances. Standing dead pines were most commonly situated on flat ground or in the mid slope in the local topography.In addition, standing dead pines contributed to substrate diversity also by commonly having charred wood and broken tops. Based on the presence of dead pine snags in different stage of transition from a recently dead pine to a kelo with silvery surface, it seems evident that the process of kelo recruitment was continuously in action in the studied landscape.Conclusions: Kelo trees are an omnipresent feature in natural pine-dominated forest landscapes with important contribution to forest structural and substrate diversity. Because of their longevity and extremely slow turnover dynamics and importance for biodiversity, protection of vulnerable kelo tree populations, and ensuring their continuous recruitment, should be of high priority in forest restoration and sustainable management. | Timo Kuuluvainen Tuomas Aakala Gergely Várkonyi | 2017 | Forest Ecosystems2017，4，3： | 2 |
| 14 | 最新无模型深度强化学习研究:从零开始训练机器人“玩乐高”显示文摘伯克利最新提出无模型深度强化学习方法——soft Q-learning(SQL)算法,该算法可以对模拟和现实世界的任务执行组合性,同时在该算法基础上提出了一种使用具有表达性的神经网络策略学习机器人操作技能的学习框架。 | Tuomas Haarnoja Vitchyr Pong Aurick Zhou Murtaza Dalal Pieter Abbeel Sergey Levine | 2018 | 机器人产业2018，，3： | 2 |
| 15 | A new governance approach for multi-firm projects: Lessons from Olkiluoto 3 and Flamanville 3 nuclear power plant projects显示文摘 | Inkeri Ruuska Tuomas Ahola Karlos Artto Giorgio Locatelli Mauro Mancini | 2010 | International Journal of Project Management2010，，6： | 2 |
| 16 | Scalable integration of nano-,and microfluidics with hybrid two-photon lithography显示文摘Nanofluidic devices have great potential for applications in areas ranging from renewable energy to human health.A crucial requirement for the successful operation of nanofluidic devices is the ability to interface them in a scalable manner with the outside world.Here,we demonstrate a hybrid two photon nanolithography approach interfaced with conventional mask whole-wafer UV-photolithography to generate master wafers for the fabrication of integrated micro and nanofluidic devices.Using this approach we demonstrate the fabrication of molds from SU-8 photoresist with nanofluidic features down to 230 nm lateral width and channel heights from micron to sub-100 nm.Scanning electron microscopy and atomic force microscopy were used to characterize the printing capabilities of the system and show the integration of nanofluidic channels into an existing microfluidic chip design.The functionality of the devices was demonstrated through super-resolution microscopy,allowing the observation of features below the diffraction limit of light produced using our approach.Single molecule localization of diffusing dye molecules verified the successful imprint of nanochannels and the spatial confinement of molecules to 200 nm across the nanochannel molded from the master wafer.This approach integrates readily with current microfluidic fabrication methods and allows the combination of microfluidic devices with locally two-photon-written nano-sized functionalities,enabling rapid nanofluidic device fabrication and enhancement of existing microfluidic device architectures with nanofluidic features. | Oliver Vanderpoorten Quentin Peter Pavan K.Challa Ulrich F.Keyser Jeremy Baumberg Clemens F.Kaminski Tuomas P.J.Knowles | 2019 | Microsystems & Nanoengineering2019，5，1： | 2 |
| 17 | Wealth effects in emerging market economies显示文摘 | Tuomas A. Peltonen Ricardo M. Sousa Isabel S. Vansteenkiste | 2012 | International Review of Economics and Finance2012，，： | 1 |
| 18 | Rhinovirus-associated wheeze during infancy and asthma development显示文摘 | TUOMAS JARTTI JAMES E G | 2011 | Curr Respir Med Rev2011，7，3： | 1 |
| 19 | Focused web crawling in the acquisition of comparable corpora 显示文摘 | Tuomas Talvensaari | 2008 | Information Retrieval2008，11，： | 1 |
| 20 | The biology of vascular endothelial growth factors 显示文摘 | Tuomas Tammela Berndt Enholm Kari Alitalo | 2005 | Cardiovascular Research2005，65，： | 1 |
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