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| 1 | A Rapid and Cost-Effective Method for Genotyping Genome-Edited Animals:A Heteroduplex Mobility Assay Using Microfluidic Capillary Electrophoresis显示文摘The recent emergence and application of engineered endonucleases have led to the development of genome editing tools capable of rapidly implementing various targeted genome editions in a wide range of species.Moreover,these novel tools have become easier to use and have resulted in a great increase of applications.Whilst gene knockout(KO) or knockin(KI) animal models are relatively easy to achieve,there is a bottleneck in the detection and analysis of these mutations.Although several methods exist to detect these targeted mutations,we developed a heteroduplex mobility assay on an automated microfluidic capillary electrophoresis system named HMA-CE in order to accelerate the genotyping process.The HMA-CE method uses a simple PCR amplification of genomic DNA(gDNA) followed by an automated capillary electrophoresis step which reveals a heteroduplexes(HD) signature for each mutation.This allows efficient discrimination of wild-type and genome-edited animals down to the single base pair level. | Vanessa Chenouard Lucas Brusselle Jean-Marie Heslan Séverine Remy Séverine Ménoret Claire Usal Laure-Hélène Ouisse Tuan Huy NGuyen Ignacio Anegon Laurent Tesson | 2016 | Journal of Genetics and Genomics2016，43，5： | 4 |
| 2 | Characterization of two rat models of cystic fibrosis—KO and F508del CFTR—Generated by Crispr-Cas9显示文摘Background: Genetically engineered animals are essential for gaining a proper understanding of the disease mechanisms of cystic fibrosis(CF). The rat is a relevant laboratory model for CF because of its zootechnical capacity, size, and airway characteristics, including the presence of submucosal glands.Methods: We describe the generation of a CF rat model(F508 del) homozygous for the p.Phe508 del mutation in the transmembrane conductance regulator(Cftr) gene. This model was compared to new Cftr-/-rats(CFTR KO). Target organs in CF were examined by histological staining of tissue sections and tooth enamel was quantified by micro-computed tomography. The activity of CFTR was evaluated by nasal potential difference(NPD) and short-circuit current measurements. The effect of VX-809 and VX-770 was analyzed on nasal epithelial primary cell cultures from F508 del rats.Results: Both newborn F508 del and Knock out(KO) animals developed intestinal obstruction that could be partly compensated by special diet combined with an osmotic laxative. The two rat models exhibited CF phenotypic anomalies such as vas deferens agenesis and tooth enamel defects. Histology of the intestine, pancreas, liver, and lungs was normal. Absence of CFTR function in KO rats was confirmed ex vivo by short-circuit current measurements on colon mucosae and in vivo by NPD, whereas residual CFTR activity was observed in F508 del rats. Exposure of F508 del CFTR nasal primary cultures to a combination of VX-809 and VX-770 improved CFTR-mediated Cl-transport.Conclusions: The F508 del rats reproduce the phenotypes observed in CFTR KO animals and represent a novel resource to advance the development of CF therapeutics. | Elise Dreano Marc Bacchetta Juliette Simonin Louise Galmiche Claire Usal Lotfi Slimani Jérémy Sadoine Laurent Tesson Ignacio Anegon Jean-Paul Concordet Aurélie Hatton Lucile Vignaud Danielle Tondelier Isabelle Sermet-Gaudelus Marc Chanson Charles-Henry Cottart | 2019 | Animal Models and Experimental Medicine2019，2，4： | 3 |
| 3 | Modification of enzymatically amplified DNA for the detection of point mutations显示文摘 | Haliassos A Chomel JC Tesson L | 1989 | Nucleic Acids Res1989，17，9： | 1 |
| 4 | Gene-environment interaction and the metabolic syndrome显示文摘 | Adamo KB Tesson F | 2008 | Novartis Found Symp2008，293，5： | 1 |
| 5 | The Origin of Human Mesenchymal Stromal Cells Dictates Their Reparative Properties显示文摘 | Nili Naftali‐Shani Ayelet Itzhaki‐Alfia Natalie Landa‐Rouben David Kain Radka Holbova Shimrit Adutler‐Lieber Natali Molotski Elad Asher Avishay Grupper Eran Millet Ariel Tessone Eyal Winkler Jens Kastrup Micha S. Feinberg Dov Zipori Meirav Pevsner‐Fischer | 2013 | Journal of the American Heart Association2013，，5： | 1 |
| 6 | Sequence stratigraphy, seismic profiles, and core of Pleistocene Deposits on the Rh?ne continental shelf显示文摘 | Gensous B Tesson M | 1996 | Sedimentary Geology1996，105，： | 1 |
| 7 | LacZ transgenic rats tolerant for beta-galactosidase: recipients for gene transfer studies using lacZ as a reporter gene显示文摘 | Menoret S Aubert D Tesson L | 2002 | Hum Gene Ther2002，13，： | 1 |
| 8 | Knockout rats generated by embryo microinjection of TALENs 显示文摘 | Tesson L Usa1 C Menoret S etal | 2011 | Nat Biotechnol2011，29，8： | 1 |
| 9 | Systemic Delivery of Bone Marrow–Derived Mesenchymal Stem Cells to the Infarcted Myocardium: Feasibility, Cell Migration, and Body Distribution显示文摘 | Israel M. Barbash Pierre Chouraqui Jack Baron Micha S. Feinberg Sharon Etzion Ariel Tessone Liron Miller Esther Guetta Dov Zipori Laurence H. Kedes Robert A. Kloner Jonathan Leor | 2003 | Circulation: Journal of the American Heart Association2003，，7： | 1 |
| 10 | Preparation of enantiomerically pure (1S,2S)-1-aminocyclopropanephosphonic acid from mthylcyclopropanone acetal via spirophonate intermediates显示文摘 | FADEL A TESSON N | 2000 | Tetrahedron: Asymmetry2000，11，9： | 1 |
| 11 | A novel mutation in the potassiumchannel gene KVLQT1 causes the Jervell and Lange-Nielsen cardioauditorysyndrome显示文摘 | Neyroud N Tesson F Denjoy I | 1997 | Nat Genet1997，15，2： | 1 |
| 12 | 查看详情显示文摘 | Charreau B Cassard A Tesson L | | 0，，： | 1 |
| 13 | In vitro and in vivo gene transfer in the rat: characterization of recombinant adenoviral vectors for rat interleukin-4 or interleukin-10cDNA显示文摘 | David A Cassard A Tesson L | 1997 | Transplant Proc1997，29，3： | 1 |
| 14 | A novel mutation in the potassium channel gene KVLQT1 causes the Jervell and LangeNielsen cardioauditory syndrome显示文摘 | Neyroud N Tesson F Denjoy I | | 0，，2： | 1 |
| 15 | A novel mutation in the potassium channel gene KVLQT1 causes the Jervell and Lange-Nielsen cardioauditory syndrome 显示文摘 | Neyroud N Tesson F Denjoy I | 1997 | Nat Genet1997，15，2： | 1 |
| 16 | Carbon monoxide inhibits TLR-induced dendritic cell immu nogenieity显示文摘 | R6my S Blancou P Tesson L Tardif V Brion R Royer P J | 2009 | J Immunol2009，182，： | 1 |
| 17 | Knockout rats gener- ated by embryo microinjection of TALENs显示文摘 | Tesson L Usal C M6noret S | 2011 | Nat Biotech- nol2011，29，8： | 1 |
| 18 | A new locus for autesomal dominant dilated cardiomyopathy identified on chromosome 6q12-q16显示文摘 | Sylvius N Tesson F Gayet C | 2001 | Am J Hum Genet2001，68，： | 1 |
| 19 | Gene-environment interaction and the metabolic syndrome显示文摘 | Adamo KB Tesson F | 2008 | Novartis Found Symp2008，29，3： | 1 |
| 20 | SubceIiular distribution of imidazoline- Guanidinium- reeeptive sites in human and rabbit liver: major local- ization to the mitochondrial outer membrane显示文摘 | TESSON F PRIP-BUUS C LEMOINE A | 1991 | J Biol Chem1991，260，： | 1 |
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