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| 1 | A Rapid and Cost-Effective Method for Genotyping Genome-Edited Animals:A Heteroduplex Mobility Assay Using Microfluidic Capillary Electrophoresis显示文摘The recent emergence and application of engineered endonucleases have led to the development of genome editing tools capable of rapidly implementing various targeted genome editions in a wide range of species.Moreover,these novel tools have become easier to use and have resulted in a great increase of applications.Whilst gene knockout(KO) or knockin(KI) animal models are relatively easy to achieve,there is a bottleneck in the detection and analysis of these mutations.Although several methods exist to detect these targeted mutations,we developed a heteroduplex mobility assay on an automated microfluidic capillary electrophoresis system named HMA-CE in order to accelerate the genotyping process.The HMA-CE method uses a simple PCR amplification of genomic DNA(gDNA) followed by an automated capillary electrophoresis step which reveals a heteroduplexes(HD) signature for each mutation.This allows efficient discrimination of wild-type and genome-edited animals down to the single base pair level. | Vanessa Chenouard Lucas Brusselle Jean-Marie Heslan Séverine Remy Séverine Ménoret Claire Usal Laure-Hélène Ouisse Tuan Huy NGuyen Ignacio Anegon Laurent Tesson | 2016 | Journal of Genetics and Genomics2016，43，5： | 4 |
| 2 | Sequence stratigraphy, seismic profiles, and core of Pleistocene Deposits on the Rh?ne continental shelf显示文摘 | Gensous B Tesson M | 1996 | Sedimentary Geology1996，105，： | 1 |
| 3 | Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR显示文摘 | Tesson L Heslan J M Menoret S | 2002 | Transgenic Res2002，11，1： | 1 |
| 4 | Nestedness in Networks: A Theoretical Model and Some Applications 显示文摘 | Konig M D Tessone C J Zenou Y | 2014 | Theoretical Eco- nomics2014，9，3： | 1 |
| 5 | Transgenic modifications of the rat genome显示文摘 | Tesson L Cozzi J Ménoret S | | 0，，05： | 1 |
| 6 | Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR显示文摘 | Tesson L Heslan JM Ménoret S | | 0，，01： | 1 |
| 7 | Genetic aspects of heart failure显示文摘 | Komajda M Charron P Tesson F | 1999 | EurJHeartFail1999，1，2： | 1 |
| 8 | Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR 显示文摘 | TESSON L HESLAN J M MENORET S | 2002 | Transgenic Res2002，11，1： | 1 |
| 9 | Stratigraphic organi- zation of Late Pleistocene deposits of the western part of the Golfe du Lion Shelf (Langedoc Shelf), western Mediterranean Sea, using high-resolution seismic and core data 显示文摘 | Tesson M Posamentier H W Gensous B | 2000 | AAPG Bulletin2000，，84： | 1 |
| 10 | Architecture and morpholo- gy of the outer segment of a mixed tide and wave-dominated-incised val- ley, revealed by HR seismic reflection profiling: the paleo-Charente River, France显示文摘 | Weber N Chaumillon E Tesson M | 2004 | Marine Geology2004，207，1234： | 1 |
| 11 | Attempted suicide among entrants to three treatment modalities for heroin dependence in the Australian treatment outcome study:prevalence and risk factors显示文摘 | Darke S Ross J Lynskey M Tesson M | 2004 | Drug and alcohol depend2004，73，： | 1 |
| 12 | Stratigraphic organization of Late Pleistocene deposits of the western part of the Golfe du Lion Shelf (Langedoc Shelf),western Mediterranean Sea,using high-resolution seismic and core data显示文摘 | Tesson M Posamentier H W Gensous B | 2000 | AAPG Bulletin2000，84，： | 1 |
| 13 | Synchronization of extended chaotic systems with long-range interactions: An analogy to levy-flight spreading of epidemics显示文摘 | Tessone C J Cencini M Torcini A | 2006 | Phys Rev Lett2006，97，22： | 1 |
| 14 | Forced regression in a sequence stratigraphic framework:Concepts,examples,and exploration significance显示文摘 | Posamentier HW Allen GP James DP Tesson M | 1992 | AAPG Bulletin1992，76，： | 1 |
| 15 | Optimization of cryopreservation procedures for ratembryos 显示文摘 | Menoret S Jean M Tesson L | 1999 | Transplant Proc1999，31，3： | 1 |
| 16 | Revisiting the steady state kinetic mechanism of glutamine-dependant asparagines synthetase from Escherichia coli显示文摘 | Tesson AB Soper TS Ciustea M | 2003 | Arch Biochem Biophys2003，413，1： | 1 |
| 17 | Review article: Genetic aspects of heart failure显示文摘 | Komajda M Charron P Tesson F | 1999 | The European Journal of Heart Failure1999，1，： | 1 |
| 18 | An tioxidant effect of Maillard reaction products:Application to a butter cookie of a competition kinetics analysis显示文摘 | Bressa F Tesson N Dalla R M | 1996 | Journal of Agricultural and Food Chemistry1996，44，3： | 1 |
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