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| 1 | Silibinin induces hepatic stellate cell cycle arrest via enhancing p53/p27 and inhibiting Akt downstream signaling protein expression显示文摘BACKGROUND: Proliferation of hepatic stellate cells(HSCs) plays a pivotal role in the progression of liver fibrosis consequent to chronic liver injury. Silibinin, a flavonoid compound,has been shown to possess anti-fibrogenic effects in animal models of liver fibrosis. This was attributed to an inhibition of cell proliferation of activated HSCs. The present study was to gain insight into the molecular pathways involved in silibinin anti-fibrogenic effect. METHODS: The study was conducted on LX-2 human stellate cells treated with three concentrations of silibinin(10, 50 and 100 μmol/L) for 24 and 96 hours. At the end of the treatment cell viability and proliferation were evaluated. Protein expression of p27, p21, p53, Akt and phosphorylated-Akt was evaluated by Western blotting analysis and Ki-67 protein expression was by immunocytochemistry. Sirtuin activity was evaluated by chemiluminescence based assay. RESULTS: Silibinin inhibits LX-2 cell proliferation in doseand time-dependent manner; we showed that silibinin upregulated the protein expressions of p27 and p53. Such regulation was correlated to an inhibition of both downstream Akt and phosphorylated-Akt protein signaling and Ki-67 protein expression. Sirtuin activity also was correlated to silibinininhibited proliferation of LX-2 cells. CONCLUSION: The anti-proliferative effect of silibinin on LX-2 human stellate cells is via the inhibition of the expressions of various cell cycle targets including p27, Akt and sirtuin signaling. | Devaraj Ezhilarasan Jonathan Evraerts Brice Sid Pedro Buc Calderon Sivanesan Karthikeyan Etienne Sokal Mustapha Najimi | 2017 | Hepatobiliary & Pancreatic Diseases International2017，16，1： | 7 |
| 2 | 多途径丝裂原激活的蛋白激酶介导一氧化氮引起的肝癌细胞凋亡显示文摘目的 研究非离子型的diazeniumdiolate类一氧化氮供体引起肝癌细胞凋亡的分子机制。方法 利用免疫印迹、免疫沉淀、凝胶阻滞实验研究一氧化氮供体处理Hep3B肝癌细胞后,丝裂原激活的蛋白激酶、AP-1的激活以及和Hep3B肝癌细胞凋亡的关系。结果 一氧化氮可引起细胞外信号调节蛋白激酶、c-jun N末端激酶和p38激酶的激活,特别是细胞外信号调节的蛋白激酶的持续激活,其中细胞外信号调节的蛋白激酶和c-jun N末端激酶的特异的阻断剂U0126和JNK抑制剂Ⅱ可阻断AP-1的激活和Hep3B细胞的凋亡,而p38激酶的阻断剂SB203580不能阻断AP-1的激活和Hep3B肝癌细胞的凋亡。结论 一氧化氮通过激活细胞外信号调节蛋白激酶、c-jun N末端激酶,进而激活AP-1而引起Hep3B肝癌细胞的凋亡。 | 任正刚 刘银坤 Ziqiu Wang Sid Kar Brian I Carr | 2004 | 肿瘤2004，24，5： | 3 |
| 3 | Role of AMPK activation in oxidative cell damage: Implications for alcohol-induced liver disease显示文摘 | Brice Sid Julien Verrax Pedro Buc Calderon | 2013 | Biochemical Pharmacology2013，，2： | 3 |
| 4 | Pseudomyxoma peritonei: A French multicentric study of 301 patients treated with cytoreductive surgery and intraperitoneal chemotherapy显示文摘 | D. Elias F. Gilly F. Quenet J.M. Bereder L. Sidéris B. Mansvelt G. Lorimier O. Glehen | 2010 | European Journal of Surgical Oncology2010，，5： | 2 |
| 5 | Image-guided endoscopic evacuation of spontaneous intracerebral hemorrhage显示文摘 | Chad M. Miller Paul Vespa Jeffrey L. Saver Chelsea S. Kidwell Stanley T. Carmichael Jeffry Alger John Frazee Sid Starkman David Liebeskind Valeriy Nenov Robert Elashoff Neil Martin | 2008 | Surgical Neurology2008，，5： | 2 |
| 6 | Ten Years of Descending Necrotizing Mediastinitis: Management of 23 Cases显示文摘 | Fabio Roccia Gian Carlo Pecorari Alberto Oliaro Ettore Passet Paolo Rossi Juri Nadalin Paolo Garzino-Demo Sid Berrone | 2007 | Journal of Oral and Maxillofacial Surgery2007，，9： | 2 |
| 7 | Role of oxidative stress in the pathogenesis of alcohol-induced liver disease显示文摘 | B. Sid J. Verrax P. B. Calderon | 2013 | Free Radical Research2013，，11： | 2 |
| 8 | Age-specific relevance of usual blood pressure to vascular mortality显示文摘 | Sid Port | 2003 | The Lancet2003，，9366： | 1 |
| 9 | Thrombospondin-1 enhances human thyroid carcinoma cell invasion through urokinase activity显示文摘 | Sid B Langlois B Sartelet H | 2008 | Int J Biochem Cell Biol2008，40，9： | 1 |
| 10 | Evaluation of Trichoderma harzianum for controlling root rot caused by Phytophthora capsici in pepper plants显示文摘 | SID AHMED A PéREZ-SáNCHEZ C EGEA C | 1999 | Plant Pathology1999，48，： | 1 |
| 11 | Numerical modelling of twin helical spring under tensile loading 显示文摘 | Sid Ali Kaoua Kamel Taibi Nacera Benghanem | 2011 | Applied Mathematical Modelling2011，35，3： | 1 |
| 12 | Condition-Specific Quality of Life 24 Months After Retropubic and Transobturator Sling Surgery for Stress Urinary Incontinence 显示文摘 | Sids L T Tennstedt S Lukacz E | 2012 | Female pelvic medicine & reconstructive surgery2012，18，5： | 1 |
| 13 | Assessing realized information systems strategy 显示文摘 | Chan E Yoland Huff Sid L Copeland Duncan G | 1998 | Journal of Strategic Information Systems1998，6，： | 1 |
| 14 | Study of the operating characteristics of a reversed absorption heat-pump system ( heat transformer)显示文摘 | Sidding Mohammed B E Watson F A | 1983 | Chemical Engineering Research Design1983，61，： | 1 |
| 15 | Selecting bac- terial strains for use in the biocontrol of diseases caused by Phytophthora capsici and Alternaria alternata in sweet pep- per plants显示文摘 | Sid A Ezziyyani M Egea-Gilabert C | 2006 | Blologia Plantarum2006，47，4： | 1 |
| 16 | Remote assay for chlorophyll photosynthetic potential of crops on the example of wheat显示文摘 | Sid'ko A F | | 0，，05： | 1 |
| 17 | Improving gastroschisis outcomes: Does birth place matter?显示文摘 | Kate B. Savoie Eunice Y. Huang Shahroz K. Aziz Martin L. Blakely Sid Dassinger Amanda R. Dorale Eileen M. Duggan Matthew T. Harting Troy A. Markel Stacey D. Moore-Olufemi Sohail R. Shah Shawn D. St. Peter Koujen Tsao Deidre L. Wyrick Regan F. Williams | 2014 | Journal of Pediatric Surgery2014，，： | 1 |
| 18 | Dig显示文摘 | Ker M D Deng C K and Huang J L SID Tech | 2005 | 288-2912005，，： | 1 |
| 19 | Selecting bacterial strains for use in the biocontrol of diseases caused by Phytophthora capsici and Alternaria alternata in sweet pepper plants 显示文摘 | SID A EZZIYYAN1 M EGEA-GILABERT C | 2003 | Blologia Plantarum2003，47，4： | 1 |
| 20 | Defining Postoperative Ileus: Results of a Systematic Review and Global Survey显示文摘 | Ryash Vather Sid Trivedi Ian Bissett | 2013 | Journal of Gastrointestinal Surgery2013，，： | 1 |
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