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| 1 | Interferon-armed RBD dimer enhances the immunogenicity of RBD for sterilizing immunity against SARS-CoV-2显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has caused a global crisis,urgently necessitating the development of safe,efficacious,convenient-to-store,and low-cost vaccine options.A major challenge is that the receptor-binding domain(RBD)-only vaccine fails to trigger long-lasting protective immunity if used alone for vaccination. | Shiyu Sun Yueqi Cai Tian-Zhang Song Yang Pu Lin Cheng Hairong Xu Jing Sun Chaoyang Meng Yifan Lin Haibin Huang Fang Zhao Silin Zhang Yu Gao Jian-Bao Han Xiao-Li Feng Dan-Dan Yu Yalan Zhu Pu Gao Haidong Tang Jincun Zhao Zheng Zhang Jiaming Yang Zhenxiang Hu Yang-Xin Fu Yong-Tang Zheng Hua Peng | 2021 | Cell Research2021，31，9： | 7 |
| 2 | Micro-droplet Digital Polymerase Chain Reaction and Real-Time Quantitative Polymerase Chain Reaction Technologies Provide Highly Sensitive and Accurate Detection of Zika Virus显示文摘The establishment of highly sensitive diagnostic methods is critical in the early diagnosis and control of Zika virus(ZIKV)and in preventing serious neurological complications of ZIKV infection. In this study, we established micro-droplet digital polymerase chain reaction(ddPCR) and real-time quantitative PCR(RT-qPCR) protocols for the detection of ZIKV based on the amplification of the NS5 gene. For the ZIKV standard plasmid, the RT-qPCR results showed that the cycle threshold(Ct) value was linear from 10~1 to 10~8 copy/l L, with a standard curve R^2 of 0.999 and amplification efficiency of 92.203%;however, a concentration as low as 1 copy/l L could not be detected. In comparison with RT-qPCR, the dd PCR method resulted in a linear range of 10~1–10~4 copy/l L and was able to detect concentrations as low as 1 copy/l L. Thus, for detecting ZIKV from clinical samples, RT-qPCR is a better choice for high-concentration samples(above 10~1 copy/l L),while ddPCR has excellent accuracy and sensitivity for low-concentration samples. These results indicate that the ddPCR method should be of considerable use in the early diagnosis, laboratory study, and monitoring of ZIKV. | Yuan Hui Zhiming Wu Zhiran Qin Li Zhu Junhe Liang Xujuan Li Hanmin Fu Shiyu Feng Jianhai Yu Xiaoen He Weizhi Lu Weiwei Xiao Qinghua Wu Bao Zhang Wei Zhao | 2018 | Virologica Sinica2018，33，3： | 6 |
| 3 | Functionally Modified Cellulose Nanocrystals as an Adsorbent for Anionic Dyes显示文摘Cellulose nanocrystal was modified with poly(N,N-diethylaminomethyl methacrylate) to prepare an adsorbent containing amine groups for removing anionic dyes from waste water. The prepared adsorbent was characterized by Fourier-transform infrared spectrometry(FT-IR), X-ray photoelectron spectroscopy(XPS), and thermogravimetric analysis(TGA). The adsorption was affected by various factors, such as the contact time, adsorbent dosage, dye solution pH value, initial dye concentration, and ionic strength. The results revealed that amine functional groups mainly contribute to the adsorption of azo dyes(AO7). The adsorbent showed pseudo-secondorder adsorption kinetics, indicating that the dye molecules were chemisorbed on the adsorbent. The adsorption isotherm was found to fit better with the Langmuir isotherm model than with the Freundlich isotherm model. | Ming Li ShiYu Fu | 2018 | Paper And Biomaterials2018，3，4： | 5 |
| 4 | Effect of Enzyme-assisted Refining on the Properties of Bleached Softwood Pulp显示文摘Enzymatic pretreatment of pulp is demonstrated to be potentially effective for decreasing the energy consumption in the refining process.Herein,a neutral cellulase was utilized for the pretreatment of bleached softwood pulp in order to improve the refining performance.Cellulase pretreatment effectively improved the drainability of the pulp and could thus reduce the energy consumption in the refining process.The beating degree of the pulp was significantly improved at 6000 PFI revolutions,at which a maximum increase of 70% could be obtained.The water retention value(WRV) of the pulp increased by 17% after treatment with cellulase at a dosage of 5 IU/g,and the fibers could be easily torn apart after enzymatic treatment.To achieve the same beating degree,the refining time could be shortened by 80% when the pulp was treated with cellulase.Using a low dosage of cellulase,the freeness of the pulp increased rapidly without deterioration of the mechanical properties. | YangYang Peng Hao Liu ShiYu Fu XinLiang Li | 2018 | Paper And Biomaterials2018，3，3： | 4 |
| 5 | Effect of Acetic Acid Prehydrolysis on Unbleached Bamboo Pulp Production显示文摘Bamboo was used as raw material for unbleached pulp production.An acetic acid prehydrolysis process was employed before the pulping process.The effect of acetic acid prehydrolysis on pulp properties was investigated.The results showed that some components,such as hemicellulose and extractives,were degraded or dissolved in the prehydrolysis process prior to kraft pulping.SEM images of the substrate after treatment indicated that the parenchyma cell wall was thinner,and the size of pores between fiber bundle cell walls was larger.The pulping results showed that acetic acid prehydrolysis could improve the pulp quality and make the pulp easier to bleach.The brightness of the pulp reached 59.6%ISO with a single oxygen delignification step.The acetic acid prehydrolysis decreased pulp viscosity and fiber length,but not significantly.The amount of parenchyma cells in the pulp was reduced,which was beneficial for papermaking and improving mechanical strength of paper.This procedure has good potential for unbleached pulp production. | YangYang Peng ChenYin Chen Ying Wang ShiYu Fu | 2018 | Paper And Biomaterials2018，3，3： | 3 |
| 6 | Preparation of Photo-thermal Cellulose Nanocrystal-based Hydrogel显示文摘Cellulose nanocrystal(CNC)prepared by hydrolysis of cotton linters with sulfuric acid was used to react with chloroauric acid to manufacture a gold nanoparticle/CNC composite.The composite was then graft-copolymerized with N-isopropylacrylamide to obtain a photo-thermal ultrafine gold nanoparticles/CNC-based hydrogel.The hydrogel was studied by performing scanning electron microscopy,and it was found that the prepared hydrogel had a network structure.The temperature of the hydrogel increased from 25℃to 39℃and its volume decreased by 30%when it was exposed to visible light(400~750 nm)for 1 h.The experiment results indicated that the prepared photo-thermal CNC-based hydrogel has thermal responsiveness and photo-thermal properties. | ZiHe Guo Tao Ma ShiYu Fu | 2019 | Paper And Biomaterials2019，4，2： | 3 |
| 7 | Performance Assessment of Nanocellulose Hydroxypropyl Methyl Cellulose Composite on Role of Nano-CaCO_(3) for the Preservation of Paper Documents显示文摘Deacidification and self-cleaning are important for the preservation of paper documents.In this study,nano-CaCO_(3) was used as a deacidification agent and stabilized by nanocellulose(CNC)and hydroxypropyl methylcellulose(HPMC)to form a uniform dispersion.Followed by polydimethylsiloxane(PDMS)treatment and chemical vapor deposition(CVD)of methyltrimethoxysilane(MTMS),a hydrophobic coating was constructed for self-cleaning purposes.The pH value of the treated paper was approximately 8.20,and the static contact angle was as high as 152.29°.Compared to the untreated paper,the tensile strength of the treated paper increased by 12.6%.This treatment method endows the paper with a good deacidification effect and self-cleaning property,which are beneficial for its long-term preservation. | Xiaochun Ma Altaf Halim Xiaohong Li Huiming Fan Shiyu Fu | 2022 | Paper And Biomaterials2022，7，2： | 2 |
| 8 | Regulating the Function of Nanocomposite Made from Hydroxypropyl Methyl Cellulose with Bacterial Cellulose Nanocrystal显示文摘Hydroxypropyl methyl cellulose(HPMC)-based hybrid nanocomposites reinforced with bacterial cellulose nanocrystals(BCNC) were prepared and characterized.The HPMC nanocomposites exhibited good thermal stability,with a thermogravimetric peak temperature of around 346℃.The addition of BCNC did not significantly affect the thermal degradation temperature or improve the transparency of HPMC nanocomposites.However,the addition of BCNC favorably affected the light scattering properties of the nanocomposites and enhanced mechanical properties such as tensile stress and Young's modulus from 65 MPa and 1.5 GPa up to 139 MPa and 3.2 GPa,respectively.The oxygen permeability of the HPMC nanocomposites also increased with increase in the amount of BCNC added. | AiJing Zhou YangYang Peng ShiYu Fu Hao Liu | 2016 | Paper And Biomaterials2016，1，2： | 2 |
| 9 | Rapid determination of methanol in black liquors by full evaporation headspace gas chromatography 显示文摘 | Li Hailong Zhan Huaiyu Fu Shiyu | 2007 | Journal of Chromatography A2007，1175，1： | 1 |
| 10 | Crosstalk between adipose-derived stem cells and chondrocytes:when growth factors matter显示文摘Adipose-derived stem cells(ASCs)and mesenchymal stem cells are promising for tissue repair because of their multilineage differentiation capacity.Our previous data confirmed that the implantation of mixed ASCs and chondrocytes into cartilage defects induced desirable in vivo healing outcomes.However,the paracrine action of ASCs on chondrocytes needs to be further elucidated.In this study,we established a co-culture system to achieve cell-to-cell and cell-to-tissue crosstalk and explored the soluble growth factors in both ASCs and chondrocytes supplemented with 1%fetal bovine serum to mimic the physiological microenvironment.In ASCs,we screened for growth factors by semi-quantitative PCR and quantitative real-time PCR and found that the expression of bone morphogenetic protein 2(BMP-2),vascular endothelial growth factor B(VEGFB),hypoxia inducible factor-1α(HIF-1α),fibroblast growth factor-2(FGF-2),and transforming growth factor-β1 significantly increased after co-culture in comparison with mono-culture.In chondrocytes,VEGFA was significantly enhanced after co-culture.Unexpectedly,the expression of collagen II and aggrecan was significantly down-regulated in the co-culture group compared with the mono-culture group.Meanwhile,among all the growth factors screened,we found that the BMP family members BMP-2,BMP-4,and BMP-5 were down-regulated and that VEGFB,HIF-1α,FGF-2,and PDGF were significantly decreased after co-culture.These results suggest that crosstalk between ASCs and chondrocytes is a pathway through the regulated growth factors that might have potential in cartilage repair and regeneration and could be useful for tissue engineering. | Juan Zhong Bin Guo Jing Xie Shuwen Deng Na Fu Shiyu Lin Guo Li Yunfeng Lin Xiaoxiao Cai | 2015 | Bone Research2015，3，4： | 1 |
| 11 | Effect of surface microfibrillation of sisal fibre on the mechanical properties of sisal/aramid fibre hybrid composites显示文摘 | Zhong Linxin Fu Shiyu Zhou Xuesong | 2011 | Composites: Part A2011，42，： | 1 |
| 12 | Colloidal stability of negatively charged cellulose nanocrystalline in aqueous systems显示文摘 | Zhong Linxin Fu Shiyu Peng Xinwen | 2012 | Carbohydrate Polymers2012，90，1： | 1 |
| 13 | Rapid determination of methanol in black liquors by full evaporation headspace gas chromatography显示文摘 | Li Hailong Zhan Huaiyu Fu Shiyu | 2007 | Journal of Chromatography A2007，1175，： | 1 |
| 14 | Magnetic Wood-based Superhydrophobic Aerogel for Efficient Oil-Water Separation显示文摘The fabrication of directionally driven oil-water separation materials has great significance for the removal of oil spills and organic pollutants.In this study,an oil-water separation aerogel capable of directionally adsorbing oil was designed using an anisotropic wood aerogel with a layered structure and a top-down fabrication strategy.Specifically,a magnetic wood-based superhydrophobic aerogel(methyltrimethoxysilane(MTMS)/Fe_(3)O_(4) wood aerogel)was developed through the in situ coprecipitation of Fe_(3)O_(4) nanoparticles and chemical vapor deposition.Owing to its highly porous structure,lipophilicity,hydrophobicity(water contact angle of 160°),and high compressibility,the MTMS/Fe_(3)O_(4) wood aerogel exhibits excellent oil-water separation performance and compression cycle stability.Additionally,the Fe_(3)O_(4) endows the material with excellent magnetic and photothermal conversion capabilities.These excellent properties make MTMS/Fe_(3)O_(4) wood aerogel a promising recyclable and sustainable oil-water separation material. | Junqing Chen Zede Yi Shiyu Fu | 2022 | Paper And Biomaterials2022，7，2： | 1 |
| 15 | Rapid determination of ethanol in fermentation liquor by full evaporation headspace gas chromatography显示文摘 | Li Hailong Chai Xin-Sheng Deng Yulin Zhan Huaiyu Fu Shiyu | 2008 | Journal of Chromatography A2008，，： | 1 |
| 16 | Preparation of Hydroxypropyl Methylcellulose Acetate Succinate with a Narrow Molecular Weight Dispersion显示文摘Hydroxypropyl methylcellulose acetate succinate(HPMCAS)was successfully synthesized from the reaction of hydroxypropyl methylcellulose with succinic anhydride and acetic anhydride in an acetone/pyridine system.Products with different contents of succinyl groups and acetyl groups were prepared by varying the reaction conditions.In the acetone/pyridine system,equipment corrosion does not occur,the product is easy to wash,and the solvent can be recycled.By varying the concentration of the esterifying agents,products with different ratios of acidic groups can be obtained.Under the optimum conditions,the obtained products had an average molecular weight between 5.39×104 and 5.41×104,a number average molecular weight from 4.97×104 to 5.13×104,and a polydispersity index from 1.05 to 1.08.The products dissolved well in acetone and methanol,and formed films on a mold.The films had good pH-sensitivity,tensile strength,and thermal stability.The formed films could dissolve in solutions with a pH value ranging from 5.4 to 6.4,and are therefore suitable for use as an enteric coating for pharmaceutical dosage forms. | Liangliang Zhang Shiyu Fu | 2020 | Paper And Biomaterials2020，5，4： | 1 |
| 17 | Superabsorbent nanocomposite hydrogels made of carboxylated cellulose nanofibrils and CMC-g-p(AA-co-AM)显示文摘 | Yiming Zhou Shiyu Fu Liangliang Zhang Huaiyu Zhan | 2013 | Carbohydrate Polymers2013，，2： | 1 |
| 18 | Investigation of the chemical basis for inefficient lignin removal in softwood kraft pulp during oxygen delignification显示文摘 | Fu Shiyu Lucian A L | | 0，，： | 1 |
| 19 | Expression of ERCC1, TYMS, RRM1, TUBB3, non-muscle myosin II, myoglobinand MyoD1 in lung adenocarcinoma pleural effusions predicts survival in patientsreceiving platinum-based chemotherapy显示文摘 | Haijiao Jiang He Wang Shiyu Wang Zhengtong Pei Zhimin Fu Changqing Fang Jian Wang Qingjie Lu Enhua Wang Jianhua Li | 2015 | Molecular Medicine Reports2015，，： | 1 |
| 20 | Etherification of Alkali-pretreated Sugarcane Bagasse Cellulose in Tetrahydrofuran显示文摘Sugarcane bagasse(SCB)is an important by-product in the sugar industry.It is a source of cellulose fibers or cellulose for paper mills and textiles factories.In this study,SCB was ethyl etherified in tetrahydrofuran(THF)after alkali pretreatment.The alkali concentration for the pretreatment,the ratio of ethyl bromide(EtBr)to dried SCB in the reaction,reaction time,and temperature were investigated for the etherification of SCB.The ethoxyl content and characterization of the product were determined using headspace gas chromatography(HS-GC),Fourier Transform Infrared(FT-IR)and 13C-NMR spectroscopy,respectively.It was found that SCB was well-etherified with EtBr in alkali-THF.Upon ethylation of SCB,the ethoxyl content of the product was high when the alkali concentration and the ratio of EtBr to dried SCB were controlled from 50%to 75%and 4:1(V/w)to 6:1(V/w),respectively.The reaction occurred optimally when the temperature was controlled below 110℃;above this temperature,the degree of etherification decreased.The thermal stability of ethylated SCB was higher than that of SCB but slightly lower than that of commercial ethyl cellulose.Ethylated SCB has the potential to form composites with many materials because it is soluble in a wide variety of solvents. | Shiyu Fu Chuanlong Xie | 2020 | Paper And Biomaterials2020，5，3： | 1 |
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