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| 1 | Micro-droplet Digital Polymerase Chain Reaction and Real-Time Quantitative Polymerase Chain Reaction Technologies Provide Highly Sensitive and Accurate Detection of Zika Virus显示文摘The establishment of highly sensitive diagnostic methods is critical in the early diagnosis and control of Zika virus(ZIKV)and in preventing serious neurological complications of ZIKV infection. In this study, we established micro-droplet digital polymerase chain reaction(ddPCR) and real-time quantitative PCR(RT-qPCR) protocols for the detection of ZIKV based on the amplification of the NS5 gene. For the ZIKV standard plasmid, the RT-qPCR results showed that the cycle threshold(Ct) value was linear from 10~1 to 10~8 copy/l L, with a standard curve R^2 of 0.999 and amplification efficiency of 92.203%;however, a concentration as low as 1 copy/l L could not be detected. In comparison with RT-qPCR, the dd PCR method resulted in a linear range of 10~1–10~4 copy/l L and was able to detect concentrations as low as 1 copy/l L. Thus, for detecting ZIKV from clinical samples, RT-qPCR is a better choice for high-concentration samples(above 10~1 copy/l L),while ddPCR has excellent accuracy and sensitivity for low-concentration samples. These results indicate that the ddPCR method should be of considerable use in the early diagnosis, laboratory study, and monitoring of ZIKV. | Yuan Hui Zhiming Wu Zhiran Qin Li Zhu Junhe Liang Xujuan Li Hanmin Fu Shiyu Feng Jianhai Yu Xiaoen He Weizhi Lu Weiwei Xiao Qinghua Wu Bao Zhang Wei Zhao | 2018 | Virologica Sinica2018，33，3： | 6 |
| 2 | Start-up of the Anammox process from the conventional activated sludge in a membrane bioreactor显示文摘 | Tao Wang Hanmin Zhang Fenglin Yang Sitong Liu Zhimin Fu Huihui Chen | 2008 | Bioresource Technology2008，，9： | 2 |
| 3 | Start-up of the Anammox process from the conventional activated sludge in a membrane bioreactor显示文摘 | Tao Wang Hanmin Zhang Fenglin Yang Sitong Liu Zhimin Fu Huihui Chen | 2008 | Bioresource Technology2008，，9： | 2 |
| 4 | The reductive characteristics of amidoxime chelate nonwovenfabric 显示文摘 | Lu Yun Fu Ruowen Zeng Hanmin | 1993 | Polym Sci(USSr)1993，35，4： | 1 |
| 5 | The Reductive Characteristic of Amidoxime Chelate Nonwoven Fabric显示文摘 | Ruowen FU Hanmin ZengJ | 1993 | Polym Science1993，35，4： | 1 |
| 6 | Start-up of the Anammox process from the conventional activated sludge in a membrane bioreactor显示文摘 | Tao Wang Hanmin Zhang Fenglin Yang Sitong Liu Zhimin Fu Huihui Chen | 2008 | Bioresource Technology2008，，9： | 1 |
| 7 | The reduction of Pt(IV) with activated carbon fibers—An XPS study显示文摘 | Ruowen Fu Hanmin Zeng Yun Lu | 1995 | Carbon1995，，5： | 1 |
| 8 | STUDIES ON THE CHEMICAL STRUCTURES OFACTIVATED CARBON FIBERS BY SOLID STATE NMR显示文摘The solid stale C13-NMR spectra of different ACFs from various precursor fiberswere recorded in this paper. The effects of activation conditions on chemical structuresOf ACFs, as well as the changes of chemical structures during carbonization and redoxreaction were investigated by NMR technique. At same time, the solid state P31-NMRspectra of ACFs are studied The C13-NMR spectra of ACFs can be divided into sixbands that are assigned to methyl and methylene groups, hydroxyl and ether groups,acetal (or methylenedioxy carbon, graphite-like aromatic carbon structure, phenol,and quinone groups, respectively. Only phosphorous pentoxide exists on ACFs andCFs. Moreover, most of them are stuck over the crystal face but not at the edge ofgraphite-like micro-crystaL The carbonization and activation conditions affect theC13-NMR spectra of ACFs. The experimental results indicate that the redox reactionof ACFs with oxidants greatly consumes C-H group. | FU Ruowen LIU Ling ZENG Hanmin HUANG Wenqiang SUN Pingchuan(Materials Science Institute, Zhongshan University Guangzhou, China2State key laboratory of functional polymer materials for adsorption and separationNankai University Tianjin, Chinacesfrw@zsulin | 1999 | Chinese Journal of Reactive Polymers1999，8，1： | 0 |
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