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| 1 | Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma显示文摘AIM:To investigate cell type specific distribution ofβ-actin expression in gastric adenocarcinoma and its correlation with clinicopathological parameters.METHODS:β-actin is a housekeeping gene,frequently used as loading control,but,differentially expresses in cancer.In gastric cancer,an overall increased expression ofβ-actin has been reported using tissue disruptive techniques.At present,no histological data is available to indicate its cell type-specific expression and distribution pattern.In the present study,we analyzedβ-actin expression and distribution in paired normal and tumor tissue samples of gastric adenocarcinoma patients using immunohistochemistry(IHC),a tissue non-disruptive technique as well as tissue disruptive techniques like reverse transcriptase-polymerase chain reaction(RT-PCR)and western blotting.Correlation ofβ-actin level with clinicopathological parameters was done using univariate analysis.RESULTS:The results of this study showed significant overexpression,at both mRNA and protein level in tumor tissues as confirmed by RT-PCR(1.47±0.13 vs2.36±0.16;P<0.001)and western blotting(1.92±0.26 vs 2.88±0.32;P<0.01).IHC revealed thatβ-actin expression is majorly distributed between epithelial and inflammatory cells of the tissues.Inflammatory cells showed a significantly higher expression compared to epithelial cells in normal(2.46±0.13 vs 5.92±0.23,P<0.001),as well as,in tumor tissues(2.79±0.24 vs6.71±0.14,P<0.001).Further,comparison of immunostaining between normal and tumor tissues revealed that both epithelial and inflammatory cells overexpressβ-actin in tumor tissues,however,significant difference was observed only in inflammatory cells(5.92±0.23vs 6.71±0.14,P<0.01).Moreover,combined expression in epithelial and inflammatory cells also showed significant increase(4.19±0.15 vs 4.75±0.14,P<0.05)in tumor tissues.In addition,univariate analysis showed a positive correlation ofβ-actin level of inflammatory cells with tumor grade(P<0.05)while epithelial cells exhibited negative correlation(P>0.05).CONCLUSION:In gastric cancer,β-actin showed an overall higher expression predominantly contributed by inflammatory or tumor infiltrating immune cells of the tissue microenvironment and correlates with tumor grade. | Shafqat A Khan Monica Tyagi Ajit K Sharma Savio G Barreto Bhawna Sirohi Mukta Ramadwar Shailesh V Shrikhande Sanjay Gupta | 2014 | World Journal of Gastroenterology2014，20，34： | 2 |
| 2 | A rare case of primary gastric plasmacytoma: an unforeseen surprise显示文摘 | Krishnamoorthy N Bal MM Ramadwar M | 2010 | J Cancer Res Ther2010，6，4： | 1 |
| 3 | A rare case of primary gastric plasmacytoma: an unforeseen surprise显示文摘 | Krishnamoorthy N Bal MM Ramadwar M | 2010 | J Cancer Res Ther2010，6，4： | 1 |
| 4 | A rare case of pri- mary gastric plasmacytoma: an unforeseen surprise 显示文摘 | Krishnamoorthy N Bal M M Ramadwar M | 2010 | J CancerRes Ther2010，6，4： | 1 |
| 5 | A study of pathologic risk factors in postehemoreduced, enueleated specimens of advanced retinoblastomas in a developing country 显示文摘 | Suryawanshi P Ramadwar M Dikshit R | 2011 | Arch Path& Lab Med2011，135，： | 1 |
| 6 | Hepatoblastoma: a single institutional experience of 18 cases显示文摘 | Parul J. Shukla Savio G. Barreto Sajid S. Qureshi Rohini Hawaldar Shailesh V. Shrikhande Mukta R. Ramadwar Shripad Banavali | 2008 | Pediatric Surgery International2008，，7： | 1 |
| 7 | Collagen-coated Vicryl mesh is not a suitable material for repair of diaphragmatic defects显示文摘 | Ramadwar R H Carachi R Young D G | 1997 | J Pediatr Surg1997，32，： | 1 |
| 8 | A study of pathologic risk factors in postchemoreduced, enucleated specimens of advanced retinoblastomas in a developing country 显示文摘 | Suryawanshi P Ramadwar M Dikshit R | 2011 | Arch Pathol Lab Med2011，135，： | 1 |
| 9 | A rare case of primary gastric plasmacytoma:ar unforeseen surprise显示文摘 | Krishnamoorthy N Bal M M Ramadwar M | 2010 | J cancer Res Ther2010，6，4： | 1 |
| 10 | Kimura disease with prolifer- ative squamous metaplasia:an unusual finding and a poten- tial diagnostic pitfall 显示文摘 | Dave B Desai S Ramadwar M | 2007 | Indian J Pathol Microhiol2007，50，4： | 1 |
| 11 | Kimura disease with prelifemtive squamous metaplasia :an unusual finding mad a potential diagnos- tic piffull显示文摘 | Dave B Desai S Ramadwar M | 2007 | Indian J pathal mierebiol2007，50，4： | 1 |
| 12 | A rare ease of primary gastric plasmacytoma:An unforeseen surpfise显示文摘 | Krishnamoorthy N Bal MM Ramadwar M | 2010 | J Cancer Res Ther2010，6，4： | 1 |
| 13 | A rare case of pri-mary gastric plasmacytoma:an unforeseen surprise显示文摘 | Krishnamoorthy N Bal M M Ramadwar M | 2010 | J Cancer ResTher2010，6，4： | 1 |
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