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| 1 | Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery显示文摘AIM: To investigate the impact of minor abdominal surgery on the caecal microbial population and on markers of gut inflammation.METHODS: Four week old piglets were randomly allocated to a no-surgery 'control' group(n = 6) or a 'transection surgery' group(n = 5).During the transection surgery procedure, a conventional midline incision of the lower abdominal wall was made and the small intestine was transected at a site 225 cm proximal to the ileocaecal valve, a 2 cm segment was removed and the intestine was re-anastomosed.Piglets received a polymeric infant formula diet throughout the study period and were sacrificed at two weeks post-surgery.Clinical outcomes including weight, stool consistency and presence of stool fat globules were monitored.High throughput DNA sequencing of colonic content was used to detect surgery-relateddisturbances in microbial composition at phylum, family and genus level.Diversity and richness estimates were calculated for the control and minor surgery groups.As disturbances in the gut microbial community are linked to inflammation we compared the gene expression of key inflammatory cytokines(TNF, IL1 B, IL18, IL12, IL8, IL6 and IL10) in ileum, terminal ileum and colon mucosal extracts obtained from control and abdominal surgery groups at two weeks post-surgery.RESULTS: Changes in the relative abundance of bacterial species at family and genus level were confined to bacterial members of the Proteobacteria and Bacteroidetes phyla.Family level compositional shifts included a reduction in the relative abundance of Enterobacteriaceae(22.95 ± 5.27 vs 2.07 ± 0.72, P < 0.01), Bacteroidaceae(2.54 ± 0.56 vs 0.86 ± 0.43, P < 0.05) and Rhodospirillaceae(0.40 ± 0.14 vs 0.00 ± 0.00, P < 0.05) following transection surgery.Similarly, at the genus level, changes associated with transection surgery were restricted to members of the Proteobacteria and Bacteroidetes phyla and included decreased relative abundance of Enterobacteriaceae(29.20 ± 6.74 vs 2.88 ± 1.08, P < 0.01), Alistipes(4.82 ± 1.73 vs 0.18 ± 0.13, P < 0.05) and Thalassospira(0.53 ± 0.19 vs 0.00 ± 0.00, P < 0.05).Surgeryassociated microbial dysbiosis was accompanied by increased gene expression of markers of inflammation.Within the ileum IL6 expression was decreased(4.46 ± 1.60 vs 0.24 ± 0.06, P < 0.05) following transection surgery.In the terminal ileum, gene expression of TNF was decreased(1.51 ± 0.13 vs 0.80 ± 0.16, P < 0.01) and IL18(1.21 ± 0.18 vs 2.13 ± 0.24, P < 0.01), IL12(1.04 ± 0.16 vs 1.82 ± 0.32, P < 0.05) and IL10(1.04 ± 0.06 vs 1.43 ± 0.09, P < 0.01) gene expression increased following transection surgery.Within the colon, IL12(0.72 ± 0.13 vs 1.78 ± 0.28, P < 0.01) and IL10(0.98 ± 0.02 vs 1.95 ± 0.14, P < 0.01) gene expression were increased following transection surgery.CONCLUSION: This study suggests that minor abdominal surgery in infants, results in long-term alteration of the colonic microbial composition and persistent gastrointestinal inflammation. | Susan Lapthorne Julie E Bines Fiona Fouhy Nicole L Dellios Guineva Wilson Sarah L Thomas Michelle Scurr Catherine Stanton Paul D Cotter Prue M Pereira-Fantini | 2015 | World Journal of Gastroenterology2015，21，14： | 3 |
| 2 | Cancer - related fatigue:A critical ap- praisal显示文摘 | Prue G Rankin J Allen J | 2006 | Eur J Can2006，42，7： | 1 |
| 3 | Optimisation of LNG mixed-refrigerant processes considering operation and design objectives显示文摘 | Prue Hatcher Rajab Khalilpour Ali Abbas | 2012 | Computers and Chemical Engineering2012，，： | 1 |
| 4 | Use of Supplements Containing Folic Acid Among Women of Childbearing Age - United States, 2007显示文摘 | Petrini J R Hamner H C Flores A L Mulinare J Prue C | 2008 | MMWR Morbidity and Mortality Weekly Report2008，，1： | 1 |
| 5 | Cancer-related fatigue:a critical appraisal 显示文摘 | Prue G Rankin J Allen J | 2006 | Eur J Cancer2006，42，7： | 1 |
| 6 | Cancer-related fatigue:A critical appraisal显示文摘 | Prue G Rankin J Allen J | 2006 | European Journal of Cancer2006，，07： | 1 |
| 7 | Altered FXR signalling is associated with bile acid dysmetabolism in short bowel syndrome-associated liver disease显示文摘 | Prue M. Pereira-Fantini Susan Lapthorne Susan A. Joyce Nicole L. Dellios Guineva Wilson Fiona Fouhy Sarah L. Thomas Michelle Scurr Colin Hill Cormac G.M. Gahan Paul D. Cotter Peter J. Fuller Winita Hardikar Julie E. Bines | 2014 | Journal of Hepatology2014，，5： | 1 |
| 8 | Developing and evaluating intercultural competence: Ethnographies of intercultural encounters显示文摘 | Prue Holmes Gillian O’Neill | 2012 | International Journal of Intercultural Relations2012，，5： | 1 |
| 9 | Morphological and functional changes in the colon after massive small bowel resection显示文摘 | Kate L. Healey Julie E. Bines Sarah L. Thomas Guineva Wilson Russell G. Taylor Magdy Sourial Prue M. Pereira-Fantini | 2010 | Journal of Pediatric Surgery2010，，8： | 1 |
| 10 | Environmental degradation of polyacrylamide I effects of artificial en- vironmental conditions: temperature, light, and PH 显示文摘 | Smith E A Prues S L Oehme F W | 1996 | Ecotoxicology Environmental Safety1996，，36： | 1 |
| 11 | Modem apprentices in college: 'something old, something new' 显示文摘 | Prue Huddleston | 1998 | Journal of Vocational Education & Training1998，50，2： | 1 |
| 12 | GLP-2 Administration Results in Increased Proliferation but Paradoxically an Adverse Outcome in a Juvenile Piglet Model of Short Bowel Syndrome显示文摘 | Prue M Pereira-Fantini Eva S Nagy Sarah L Thomas Russell G Taylor Magdy Sourial Monique CJ Paris Jens J Holst Peter J Fuller Julie E Bines | 2008 | Journal of Pediatric Gastroenterology and Nutrition2008，，1： | 1 |
| 13 | Assessing the needs of informal caregiv- ers to cancer survivors: a review of the instruments 显示文摘 | Prue G Santin O Porter S | 2015 | Psy- chooncology2015，24，2： | 1 |
| 14 | Using the transcription factor inhibitor of DNA biding 1 to selectively target endothelial progenitor cells offers novel strategies to inhibit tumor angiogenesis and growth 显示文摘 | Albert SM Prue NP Daniel JN | 2010 | Cancer Res2010，70，18： | 1 |
| 15 | S100A12 provokes mast cell activation: A potential amplification pathway in asthma and innate immunity显示文摘 | Zheng Yang Wei Xing Yan Hong Cai Nicodemus Tedla Chris Armishaw Nick Di Girolamo Hong Wei Wang Taline Hampartzoumian Jodie L. Simpson Peter G. Gibson John Hunt Prue Hart J. Margaret Hughes Michael A. Perry Paul F. Alewood Carolyn L. Geczy | 2007 | The Journal of Allergy and Clinical Immunology2007，，1： | 1 |
| 16 | Standards, Competence and Knowledge: Initial Teacher Training and Business显示文摘 | Ian Abbott Prue Huddleston | 2000 | International Journal of Value-Based Management2000，，3： | 1 |
| 17 | Preconception health: awareness, planning,and communication among a sample of US men and women 显示文摘 | Mitchell EW Levis DM Prue CE | 2012 | Matem Child Health J2012，16，1： | 1 |
| 18 | Acute effects of whole-body vibration on muscle activity, strength, and power 显示文摘 | PRUE CORMIE RUSSELL S DEANE N | 2006 | J Strength Conditioning Res2006，20，2： | 1 |
| 19 | Acute effects of whole-body vibration on muscle activity, strength, and power 显示文摘 | Prue Cormie Russell S Deane N | 2006 | Journal of Strength and Conditioning Research2006，20，2： | 1 |
| 20 | Self - efficacy, aftercare and relapse in a treatment program from alcoholics显示文摘 | Prue DM Rapp SR | 1992 | Journal of Studies on Alcohol1992，53，5： | 1 |
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