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| 1 | Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery显示文摘AIM: To investigate the impact of minor abdominal surgery on the caecal microbial population and on markers of gut inflammation.METHODS: Four week old piglets were randomly allocated to a no-surgery 'control' group(n = 6) or a 'transection surgery' group(n = 5).During the transection surgery procedure, a conventional midline incision of the lower abdominal wall was made and the small intestine was transected at a site 225 cm proximal to the ileocaecal valve, a 2 cm segment was removed and the intestine was re-anastomosed.Piglets received a polymeric infant formula diet throughout the study period and were sacrificed at two weeks post-surgery.Clinical outcomes including weight, stool consistency and presence of stool fat globules were monitored.High throughput DNA sequencing of colonic content was used to detect surgery-relateddisturbances in microbial composition at phylum, family and genus level.Diversity and richness estimates were calculated for the control and minor surgery groups.As disturbances in the gut microbial community are linked to inflammation we compared the gene expression of key inflammatory cytokines(TNF, IL1 B, IL18, IL12, IL8, IL6 and IL10) in ileum, terminal ileum and colon mucosal extracts obtained from control and abdominal surgery groups at two weeks post-surgery.RESULTS: Changes in the relative abundance of bacterial species at family and genus level were confined to bacterial members of the Proteobacteria and Bacteroidetes phyla.Family level compositional shifts included a reduction in the relative abundance of Enterobacteriaceae(22.95 ± 5.27 vs 2.07 ± 0.72, P < 0.01), Bacteroidaceae(2.54 ± 0.56 vs 0.86 ± 0.43, P < 0.05) and Rhodospirillaceae(0.40 ± 0.14 vs 0.00 ± 0.00, P < 0.05) following transection surgery.Similarly, at the genus level, changes associated with transection surgery were restricted to members of the Proteobacteria and Bacteroidetes phyla and included decreased relative abundance of Enterobacteriaceae(29.20 ± 6.74 vs 2.88 ± 1.08, P < 0.01), Alistipes(4.82 ± 1.73 vs 0.18 ± 0.13, P < 0.05) and Thalassospira(0.53 ± 0.19 vs 0.00 ± 0.00, P < 0.05).Surgeryassociated microbial dysbiosis was accompanied by increased gene expression of markers of inflammation.Within the ileum IL6 expression was decreased(4.46 ± 1.60 vs 0.24 ± 0.06, P < 0.05) following transection surgery.In the terminal ileum, gene expression of TNF was decreased(1.51 ± 0.13 vs 0.80 ± 0.16, P < 0.01) and IL18(1.21 ± 0.18 vs 2.13 ± 0.24, P < 0.01), IL12(1.04 ± 0.16 vs 1.82 ± 0.32, P < 0.05) and IL10(1.04 ± 0.06 vs 1.43 ± 0.09, P < 0.01) gene expression increased following transection surgery.Within the colon, IL12(0.72 ± 0.13 vs 1.78 ± 0.28, P < 0.01) and IL10(0.98 ± 0.02 vs 1.95 ± 0.14, P < 0.01) gene expression were increased following transection surgery.CONCLUSION: This study suggests that minor abdominal surgery in infants, results in long-term alteration of the colonic microbial composition and persistent gastrointestinal inflammation. | Susan Lapthorne Julie E Bines Fiona Fouhy Nicole L Dellios Guineva Wilson Sarah L Thomas Michelle Scurr Catherine Stanton Paul D Cotter Prue M Pereira-Fantini | 2015 | World Journal of Gastroenterology2015，21，14： | 3 |
| 2 | Reactivation of tuberculosis and vitamin D deficiency: the contribution of diet and exposure to sunlight 显示文摘 | Sita-Lumsden A Lapthorn G Swaminathan R | 2007 | Thorax2007，62，11： | 1 |
| 3 | Forced evolution of a herbicide detoxifying glutathione trasnsferase显示文摘 | Dixon D P McEwen A G Lapthorn A J | 2003 | Biological Chemistry2003，278，23： | 1 |
| 4 | 短肠相关性肝病与FXR信号通路有关?显示文摘【据《Hepatol》2014年7月报道】题:短肠相关洼肝病出现胆汁酸代谢障碍与FXR信号改变有关(作者Pereira-FantiniPM等)
短肠综合征(short bowel syndrome,SBS)有高达20%~30%的死亡率,虽然其高死亡率与SBS相关性肝病(SBS-ALD)有磁,但人们对于SBS—ALD的发病机制却了解不多。肠外营养(PN)被公认为是造成肝脏相关性疾病的一个关键因素,但并不是所有患者均接受了PN。对于小肠切除(small bowel resection,SBR)的儿童和成人来说,肠衰竭是出现相关性肝脏疾病的唯一独立危险因素。 | 刘洁 Pereira-Fantini PM Lapthorne S Joyce SA | 2014 | 中华普通外科学文献(电子版)2014，8，5： | 1 |
| 5 | Plant glutathione transferases 显示文摘 | Dixon D P Lapthorn A Edwards R | 2002 | Genome Biol2002，3，3： | 1 |
| 6 | Plant glutathione transferases显示文摘 | Dixon D P Lapthorn A Edwards R | 2002 | Genome Biol2002，3，30： | 1 |
| 7 | Altered FXR signalling is associated with bile acid dysmetabolism in short bowel syndrome-associated liver disease显示文摘 | Prue M. Pereira-Fantini Susan Lapthorne Susan A. Joyce Nicole L. Dellios Guineva Wilson Fiona Fouhy Sarah L. Thomas Michelle Scurr Colin Hill Cormac G.M. Gahan Paul D. Cotter Peter J. Fuller Winita Hardikar Julie E. Bines | 2014 | Journal of Hepatology2014，，5： | 1 |
| 8 | The three-dimensional structure of shikimate kinase显示文摘 | Coggins J R Lapthorn A J | 1998 | J Mol Biol1998，278，： | 1 |
| 9 | Forced evolution of a herbicide detoxifying glutathione transferase 显示文摘 | Dixon D P Mcewen A G Lapthorn AJ | 2003 | J Biol Chem2003，278，23： | 1 |
| 10 | Plant glutathine transferases显示文摘 | Dixon D P Lapthorn A Edwards R | 2002 | Genome Biol2002，3，： | 1 |
| 11 | Application of a new vector system for efficient protein purification in the crystallization of PA5104/ORF from Pseudomonas aeruginosa显示文摘 | Lohkamp B Paterson N G Lapthorn A J | 2006 | J Struct Biol2006，155，1： | 1 |
| 12 | Reactivation of tuberculosis and vitamin D deficiency: the contribution of diet and exposure to sunlight 显示文摘 | SITA-LUMSDEN A LAPTHORN G SWAMINATHAN R | 2007 | Thorax2007，62，： | 1 |
| 13 | Gut microbial diversity is reduced and is associated with colonic inflammation in a piglet model of short bowel syndrome 显示文摘 | Lapthorne S Pereira-Fantini PM Fouhy F | 2013 | Gut Microbes2013，4，3： | 1 |
| 14 | Plant glutathione transferases显示文摘 | Dixon D P Lapthorn A Edwards R | 2002 | Genome Biol2002，3，： | 1 |
| 15 | Plant glutathione transferases显示文摘 | Dixon DP Lapthorn A Edwards R | 2002 | Genome Biology2002，2，3： | 1 |
| 16 | Forced evolution of a herbicide detoxifying glutathione transferase显示文摘 | Dixon D P McEwen A G Lapthorn A J | 2003 | J Biol Chem2003，278，： | 1 |
| 17 | Crystal structre of human chorionic gonadotropin 显示文摘 | Lapthorn AJ Harris DC Littlejohn A | 1994 | Nature1994，369，6480： | 1 |
| 18 | Circularly-towed lumped mass cable model validation from experimental data显示文摘 | WILLIAMS P LAPTHORNE P TRIVAILO P | 2006 | AIAA Paper2006，，： | 1 |
| 19 | Crystal structure of human chorionic gonadotropin显示文摘 | Lapthorn AJ Harris DC Littlejohn A | 1994 | Nature1994，369，6480： | 1 |
| 20 | Crystal structure of human chorionic gonadotropin显示文摘 | A J Lapthorn D C Harris A Littlejohn | 1994 | Nature1994，369，： | 1 |
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