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| 1 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 2 | Effect of six kinds of scale inhibitors on calcium carbonate precipitation in high salinity wastewater at high temperatures显示文摘Precipitation of calcium carbonate(Ca CO3) scale on heat transfer surfaces is a serious and expensive problem widely occurring in numerous industrial processes. In this study, we compared the scale inhibition effect of six kinds of commercial scale inhibitors and screened out the best one(scale inhibitor SQ-1211) to investigate its scale inhibition performance in highly saline conditions at high temperature through static scale inhibition tests. The influences of scale inhibitor dosage, temperature, heating time and p H on the inhibition efficiency of the optimal scale inhibitor were investigated. The morphologies and crystal structures of the precipitates were characterized by Scanning Electron Microscopy and X-ray Diffraction analysis. Results showed that the scale inhibition efficiency of the optimal scale inhibitor decreased with the increase of the reaction temperature. When the concentration of Ca2+was 1600 mg/L, the scale inhibition rate could reach 90.7% at 80°C at p H 8. The optimal scale inhibitor could effectively retard scaling at high temperature. In the presence of the optimal scale inhibitor, the main crystal structure of Ca CO3 changed from calcite to aragonite. | Xiaochen Li Baoyu Gao Qinyan Yue Defang Ma Hongyan Rong Pin Zhao Pengyou Teng | 2015 | Journal of Environmental Sciences2015，27，3： | 15 |
| 3 | Effect of endotoxin on fibronectin synthesis of rat primary cultured hepatocytes显示文摘EfectofendotoxinonfibronectinsynthesisofratprimaryculturedhepatocytesJIAJinBin,HANDeWu,XURuiLing,GAOFei,ZHAOLongFeng,ZHA... | JIA JinBin, HAN DeWu, XU RuiLing, GAO Fei, ZHAO LongFeng, ZHAO YuanChang, YAN JinPin and MA XueHuiKeywords liver cirrhosis endotoxins fibronectin/analysis liver/cytology cells, cultured lipopolysaccharide | 1998 | World Journal of Gastroenterology1998，4，4： | 14 |
| 4 | Sequential infection with H1N1 and SARS-CoV-2 aggravated COVID-19 pathogenesis in a mammalian model, and covaccination as an effective method of prevention of COVID-19 and influenza显示文摘Influenza A virus may circulate simultaneously with the SARS-CoV-2 virus,leading to more serious respiratory diseases during this winter.However,the influence of these viruses on disease outcome when both influenza A and SARS-CoV-2 are present in the host remains unclear.Using a mammalian model,sequential infection was performed in ferrets and in K18-MCE2 mice,with SARS-CoV-2 infection following H1N1.We found that co-infection with H1N1 and SARS-CoV-2 extended the duration of clinical manifestation of COVID-19,and enhanced pulmonary damage,but reduced viral shedding of throat swabs and viral loads in the lungs of ferrets.Moreover,mortality was increased in sequentially infected mice compared with single-infection mice.Compared with singlevaccine inoculation,co-inoculation of PiCoVacc(a SARS-CoV-2 vaccine)and the flu vaccine showed no significant differences in neutralizing antibody titers or virus-specific immune responses.Combined immunization effectively protected K18-MCE2 mice against both H1N1 and SARS-CoV-2 infection.Our findings indicated the development of systematic models of co-infection of H1N1 and SARS-CoV-2,which together notably enhanced pneumonia in ferrets and mice,as well as demonstrated that simultaneous vaccination against HINT and SARS-CoV-2 may be an effective prevention strategy for the coming winter. | Linlin Bao Wei Deng Feifei Qi Qi Lv Zhiqi Song Jiangning Liu Hong Gao Qiang Wei Pin Yu Yanfeng Xu Yajin Qu Fengdi Li Jing Xue Shuran Gong Mingya Liu Guanpeng Wang Shunyi Wang Binbin Zhao Bin Cong Chuan Qin | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 4 |
| 5 | Intelligent modelling of clay compressibility using hybrid meta-heuristic and machine learning algorithms显示文摘Compression index Ccis an essential parameter in geotechnical design for which the effectiveness of correlation is still a challenge.This paper suggests a novel modelling approach using machine learning(ML)technique.The performance of five commonly used machine learning(ML)algorithms,i.e.back-propagation neural network(BPNN),extreme learning machine(ELM),support vector machine(SVM),random forest(RF)and evolutionary polynomial regression(EPR)in predicting Cc is comprehensively investigated.A database with a total number of 311 datasets including three input variables,i.e.initial void ratio e0,liquid limit water content wL,plasticity index Ip,and one output variable Cc is first established.Genetic algorithm(GA)is used to optimize the hyper-parameters in five ML algorithms,and the average prediction error for the 10-fold cross-validation(CV)sets is set as thefitness function in the GA for enhancing the robustness of ML models.The results indicate that ML models outperform empirical prediction formulations with lower prediction error.RF yields the lowest error followed by BPNN,ELM,EPR and SVM.If the ranges of input variables in the database are large enough,BPNN and RF models are recommended to predict Cc.Furthermore,if the distribution of input variables is continuous,RF model is the best one.Otherwise,EPR model is recommended if the ranges of input variables are small.The predicted correlations between input and output variables using five ML models show great agreement with the physical explanation. | Pin Zhang Zhen-Yu Yin Yin-Fu Jin Tommy HTChan Fu-Ping Gao | 2021 | Geoscience Frontiers2021，12，1： | 3 |
| 6 | Root-associated(rhizosphere and endosphere) microbiomes of the Miscanthus sinensis and their response to the heavy metal contamination显示文摘The plant root-associated microbiomes, including both the rhizosphere and the root endosphere microbial community, are considered as a critical extension of the plant genome. Comparing to the well-studied rhizosphere microbiome, the understanding of the root endophytic microbiome is still in its infancy. Miscanthus sinensis is a pioneering plant that could thrive on metal contaminated lands and holds the potential for phytoremediation applications. Characterizing its root-associated microbiome, especially the root endophytic microbiome, could provide pivotal knowledge for phytoremediation of mine tailings. In the current study, M. sinensis residing in two Pb/Zn tailings and one uncontaminated site were collected. The results demonstrated that the metal contaminant fractions exposed strong impacts on the microbial community structures. Their influences on the microbial community, however, gradually decreases from the bulk soil through the rhizosphere soil and finally to the endosphere, which resulting in distinct root endophytic microbial community structures compared to both the bulk and rhizosphere soil. Diverse members affiliated with the order Rhizobiales was identified as the core microbiome residing in the root of M. sinensis. In addition, enrichment of plant-growth promoting functions within the root endosphere were predicted, suggesting the root endophytes may provide critical services to the host plant. The current study provides new insights into taxonomy and potential functions of the root-associated microbiomes of the pioneer plant, M. sinensis, which may facilitate future phytoremediation practices. | Xiaoxu Sun Benru Song Rui Xu Miaomiao Zhang Pin Gao Hanzhi Lin Weimin Sun | 2021 | Journal of Environmental Sciences2021，33，6： | 3 |
| 7 | Fatty acid fouling of forward osmosis membrane:Effects of pH,calcium,membrane orientation,initial permeate flux and foulant composition显示文摘Octanoic acid(OA) was selected to represent fatty acids in effluent organic matter(EOM). The effects of feed solution(FS) properties, membrane orientation and initial permeate flux on OA fouling in forward osmosis(FO) were investigated. The undissociated OA formed a cake layer quickly and caused the water flux to decline significantly in the initial 0.5 hr at unadjusted p H 3.56; while the fully dissociated OA behaved as an anionic surfactant and promoted the water permeation at an elevated p H of 9.00. Moreover, except at the initial stage, the sudden decline of water flux(meaning the occurrence of severe membrane fouling) occurred in two conditions: 1.0.5 mmol/L Ca2+, active layer facing draw solution(AL-DS) and 1.5 mol/L Na Cl(DS); 2. No Ca2+,active layer-facing FS(AL-FS) and 4 mol/L Na Cl(DS). This demonstrated that cake layer compaction or pore blocking occurred only when enough foulants were absorbed into the membrane surface, and the water permeation was high enough to compact the deposit inside the porous substrate. Furthermore, bovine serum albumin(BSA) was selected as a co-foulant.The water flux of both co-foulants was between the fluxes obtained separately for the two foulants at p H 3.56, and larger than the two values at p H 9.00. This manifested that, at p H 3.56,BSA alleviated the effect of the cake layer caused by OA, and OA enhanced BSA fouling simultaneously; while at p H 9.00, the mutual effects of OA and BSA eased the membrane fouling. | Pin Zhao Baoyu Gao Qinyan Yue Pan Liu Ho Kyong Shon | 2016 | Journal of Environmental Sciences2016，28，8： | 2 |
| 8 | Enhancing sensitivity of microbial fuel cell sensors for low concentration biodegradable organic matter detection:Regulation of substrate concentration,anode area and external resistance显示文摘The relatively low sensitivity is an important reason for restricting the microbial fuel cell(MFC)sensors'application in low concentration biodegradable organic matter(BOM)detection.The startup parameters,including substrate concentration,anode area and external resistance,were regulated to enhance the sensitivity of MFC sensors.The results demonstrated that both the substrate concentration and anode area were positively correlated with the sensitivity of MFC sensors,and an external resistance of 210Ωwas found to be optimal in terms of sensitivity of MFC sensors.Optimized MFC sensors had lower detection limit(1 mg/L)and higher sensitivity(Slope value of the linear regression curve was 1.02),which effectively overcome the limitation of low concentration BOM detection.The essential reason is that optimized MFC sensors had higher coulombic efficiency,which was beneficial to improve the sensitivity of MFC sensors.The main impact of the substrate concentration and anode area was to regulate the proportion between electrogens and nonelectrogens,biomass and living cells of the anode biofilm.The external resistance mainly affected the morphology structure and the proportion of living cells of the anode.This study demonstrated an effective way to improve the sensitivity of MFC sensors for low concentration BOM detection. | Yangyang Gao Sha Wang Fengjun Yin Pin Hu Xingzu Wang Yuan Liu Hong Liu | 2021 | Journal of Environmental Sciences2021，33，3： | 2 |
| 9 | Integrated histopathological,lipidomic,and metabolomic profiles reveal mink is a useful animal model to mimic the pathogenicity of severe COVID-19 patients显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is transmitted on mink farms between minks and humans in many countries.However,the systemic pathological features of SARS-CoV-2-infected minks are mostly unknown.Here,we demonstrated that minks were largely permissive to SARS-CoV-2,characterized by severe and diffuse alveolar damage,and lasted at least 14 days post inoculation(dpi).We first reported that infected minks displayed multiple organ-system lesions accompanied by an increased inflammatory response and widespread viral distribution in the cardiovascular,hepatobiliary,urinary,endocrine,digestive,and immune systems.The viral protein partially co-localized with activated Mac-2+macrophages throughout the body.Moreover,we first found that the alterations in lipids and metabolites were correlated with the histological lesions in infected minks,especially at 6 dpi,and were similar to that of patients with severe and fatal COVID-19.Particularly,altered metabolic pathways,abnormal digestion,and absorption of vitamins,lipids,cholesterol,steroids,amino acids,and proteins,consistent with hepatic dysfunction,highlight metabolic and immune dysregulation.Enriched kynurenine in infected minks contributed to significant activation of the kynurenine pathway and was related to macrophage activation.Melatonin,which has significant anti-inflammatory and immunomodulating effects,was significantly downregulated at 6 dpi and displayed potential as a targeted medicine.Our data first illustrate systematic analyses of infected minks to recapitulate those observations in severe and fetal COVID-19 patients,delineating a useful animal model to mimic SARS-CoV-2-induced systematic and severe pathophysiological features and provide a reliable tool for the development of effective and targeted treatment strategies,vaccine research,and potential biomarkers. | Zhiqi Song Linlin Bao Wei Deng Jiangning Liu Erjun Ren Qi Lv Mingya Liu Feifei Qi Ting Chen Ran Deng Fengdi Li Yunpeng Liu Qiang Wei Hong Gao Pin Yu Yunlin Han Wenjie Zhao Junjun Zheng Xujian Liang Fuhe Yang Chuan Qin | 2022 | Signal Transduction and Targeted Therapy2022，7，2： | 2 |
| 10 | High Ba-Sr adakitic charnockite suite from the Nagercoil Block,southern India:Vestiges of Paleoproterozoic arc and implications for Columbia to Gondwana显示文摘The Nagercoil block is the southernmost crustal segment of the Southern Granulite Terrane(SGT)in India and is mainly composed of charnockitic rocks and felsic gneisses(charnockite suite).In this study,we present petrologic,geochemical,zircon U-Pb,REE,and Hf isotopic studies on the charnockites and leucogneiss from the Nagercoil block.Based on field investigations and petrologic studies,the charnockites can be divided into garnet-bearing and garnet-absent anhydrous granulite facies rocks with orthopyroxene.The charnockites and leucogneiss show transition from adakites to non-adakitic magmatic rocks,with enrichment in LREEs(light rare earth elements)and LILEs(large ion lithophile elements),and depletion in HREEs(heavy rare earth elements)and HFSEs(high field strength elements).Some of the charnockites and the leucogneiss show typical HSA(high silica adakite)characters,(high SiO_(2),Al_(2)O_(3),Ba-Sr,La/Yb,and Sr/Y).The HSA is considered to have formed from the interaction of slab derived melts and peridotitic mantle wedge.The high Ba-Sr features were possibly inherited from subducted oceanic crust melting under high thermal gradient during Precambrian.The magmas were underplated and subjected to fractional crystallization.Zircon grains from the charnockite and leucogneiss show zoned magmatic cores surrounded by structureless metamorphic rims.Magmatic zircon grains from the charnockites show ages ranging from 1983±8.8 Ma to 2046±14 Ma,and the metamorphic domains show an age range of 502±14 Ma to 547±8.7 Ma.Zircon from the leucogneiss yielded magmatic and metamorphic ages of 1860±20 Ma and 575.6±8.8 Ma.Both charnockites and leucogneiss show two prominent age peaks at 1987 Ma and 568 Ma.The REE data of the zircon grains show LREE depletion and HREE enrichment,with the metamorphic grains showing more depletion in HREE.Zircon Hf isotopic data of the magmatic cores of zircon grains from the charnockite yieldedε_(Hf)(t)values from-1.17 to 0.46 with T_(DM)and T_(DM)~C and age peaks at 2392 Ma and 2638 Ma,suggesting Neoarchean to Paleoproterozoic juvenile sources.We suggest that the high Ba-Sr adakitic charnockite suite from the Nagercoil block formed in a Paleoproterozoic magmatic arc setting during the assembly of the Columbia supercontinent,and underwent high-grade metamorphism associated with the amalgamation of the Gondwana supercontinent during the late Neoproterozoic-Cambrian.Our study provides new insights into the vestiges of Columbia fragments within the Gondwana assembly with two distinct cycles of crustal evolution. | Pin Gao M.Santosh Cheng-Xue Yang Sanghoon Kwon Mu.Ramkumar | 2021 | Geoscience Frontiers2021，12，3： | 2 |
| 11 | Sequential immunizations confer cross-protection against variants of SARS-CoV-2,including Omicron in Rhesus macaques显示文摘Variants of concern(VOCs)like Delta and Omicron,harbor a high number of mutations,which aid these viruses in escaping a majority of known SARS-CoV-2 neutralizing antibodies(NAbs).In this study,Rhesus macaques immunized with 2-dose inactivated vaccines(Coronavac)were boosted with an additional dose of homologous vaccine or an RBD-subunit vaccine,or a bivalent inactivated vaccine(Beta and Delta)to determine the effectiveness of sequential immunization.The booster vaccination significantly enhanced the duration and levels of neutralizing antibody titers against wild-type. | Wei Deng Qi Lv Fengdi Li Jiangning Liu Zhiqi Song Feifei Qi Qiang Wei Pin Yu Mingya Liu Shasha Zhou Yaqing Zhang Hong Gao Nan Wang Zijing Jia Kai Gao Jiayi Liu Chong Xiao Haiquan Shang Xiangxi Wang Linlin Bao Chuan Qin | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 2 |
| 12 | Rapid,Catalytic Hydrolysis of DNA by Copper (Ⅱ) Complexes显示文摘IntroductionNucleaseswhichcatalyzethehydrolyticcleavageofthephosphodiesterbackboneofDNAandRNAplayanimportantroleinthemolecula... | REN Rui, YANG Pin** and GAO Dong (Institute of Coordination Chemistry, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing, 210093 Institute of Molecular Science, Shanxi University, Taiyuan, 030006) | 1999 | Chemical Research in Chinese Universities1999，15，2： | 2 |
| 13 | SARS-CoV-2 crosses the blood-brain barrier accompanied with basement membrane disruption without tight junctions alteration显示文摘SARS-CoV-2 has been reported to show a capacity for invading the brains of humans and model animals.However,it remains undear whether and how SARS-CoV-2 crosses the blood-brain barrier(BBB).Herein,SARS-CoV-2 RNA was occasionally detected in the vascular wall and perivascular space,as well as in brain microvascular endothelial cells(BMECs)in the infected K18-hACE2 transgenic mice.Moreover,the permeability of the infected vessel was in creased.Furthermore,disin tegrity of BBB was discovered in the infected hamsters by administration of Evans blue.Interestingly,the expression of claudin5,ZO-1,occludin and the ultrastructure of tight junctions(TJs)showed unchanged,whereas,the basement membrane was disrupted in the infected animals.Using an in vitro BBB model that comprises primary BMECs with astrocytes,SARS-CoV-2 was found to infect and cross through the BMECs.Consistent with in vivo experiments,the expression of MMP9 was increased and collagen IV was decreased while the markers for TJs were not altered in the SARS-CoV-2-infected BMECs.Besides,inflammatory responses including vasculitis,glial activation,and upregulated inflammatory factors occurred after SARS-CoV-2 infection.Overall,our results provide evidence supporting that SARS-CoV-2 can cross the BBB in a transcellular pathway accompanied with basement membrane disrupted without obvious alteration of TJs. | Ling Zhang Li Zhou Linlin Bao Jiangning Liu Hua Zhu Qi Lv Ruixue Liu Wei Chen Wei Tong Qiang Wei Yanfeng Xu Wei Deng-Hong Gao Jing Xue Zhiqi Song Pin Yu Yunlin Han Yu Zhang Xiuping Sun Xuan Yu Chuan Qin | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 2 |
| 14 | Correlation of tetracycline and sulfonamide antibiotics with corresponding resistance genes and resistant bacteria in a conventional municipal wastewater treatment plant显示文摘 | Pin Gao Mariya Munir Irene Xagoraraki | 2012 | Science of the Total Environment2012，，： | 2 |
| 15 | Contribution of MDR1 gene polymorphisms on IBD predisposition and response to glucocorticoids in IBD in a C hinese population显示文摘 | Qing Fan Yang Bai Li Chen Qing Sen Zhang Zhen Hua Zhu Bin Hu Yao He Xiang Gao Yi Ming Wang Pin Jin Hu Min Hu Chen Zhi Rong Zeng | 2015 | Journal of Digestive Diseases2015，，1： | 1 |
| 16 | Synthsis,Characterization and Antibacterial Activity of Novel Fe( III ), Co(Ⅱ) and Zn(Ⅱ) Complexs with Nornoxaein 显示文摘 | GAO Fei YANG Pin XIE J un | 1995 | J Inorg Biochem1995，60，： | 1 |
| 17 | Preparation and characterization of activated carbon produced from rice straw by (NH4)2HPO4 activation显示文摘 | Gao Pin Liu Zhenhong Xue Gang | 2011 | Bioresource Technology2011，102，3： | 1 |
| 18 | Principles of Biological Abiochemistry显示文摘 | Yang Pin Gao Fei | 2000 | Beijing :Higher Education Press2000，，8： | 1 |
| 19 | Correlation of tetracycline and sulfonamide antibiotics with corresponding resistance genes and resistant bacteria in a conventional municipal wastewater treatment plant显示文摘 | Pin Gao Mariya Munir Irene Xagoraraki | 2012 | Science of the Total Environment2012，，： | 1 |
| 20 | Identification of suppressor of cytokine signalling (SOCS) 6, 7, 9 and CISH in rainbow trout Oncorhynchus mykiss and analysis of their expression in relation to other known trout SOCS显示文摘 | Tiehui Wang Qian Gao Pin Nie Christopher J. Secombes | 2010 | Fish and Shellfish Immunology2010，，4： | 1 |
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