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| 1 | Organelle pH in the Arabidopsis Endomembrane System显示文摘细胞内部的分隔空间的 pH 为房间的生存能力是必要的。尽管有它的关联,很少对这些分隔空间的 pH 被知道。到在 vivo 的措施 pH,我们首先由联合修改溶解度的绿色的改进溶解度的特征产生了二个 pH 传感器荧光灯有 pHluorins 和 codon 的察觉到 pH 能力的蛋白质(GFP )(smGFP ) 在 Arabidopsis 为表示优化了。当 pH 与荧光消失在 pH 被降低, PEpHluorin (plant-solubility-modified 黄道 pHluorin ) 逐渐地失去荧光 6.2 并且 PRpHluorin (plant-solubility-modified ratiomatric pHluorin ) ,一个双刺激的传感器,允许精确大小。分隔空间特定的传感器被进一步熔化特定的排序的信号到 PEpHluorin 和 PRpHluorin 产生。我们的结果证明 cytosol 和原子核的 pH 是类似的(pH 7.3 和 7.2 ) 当 peroxisomes, mitochondrial 矩阵,和 plastidial 基质有碱的 pH 时。能分泌的小径的分隔空间揭示渐渐的使发酸,从 pH 跨越 7.1 在 endoplasmic 蜂窝胃(嗯) 到 pH 5.2 在液泡。令人惊讶地,在 trans-Golgi 网络(TGN ) 的 pH 和 multivesicular 身体(MVB ) 是,与 pH 6.3 和 6.2,相当类似。有 concanamycin A (ConcA ) 的 vacuolar 类型 H+-ATPase (V-ATPase ) 的抑制在 TGN 和液泡在 pH 引起了激烈的增加。总的来说, PEpHluorin 和 PRpHluorin 分别地是为在 vivo 的 pH 的可视化和 quantification 的优秀 pH 传感器。 | Jinbo Shen Yonglun Zeng Xiaohong Zhuang Lei Sun Xiaoqiang Yao Peter Pimpl Liwen Jiang | 2013 | Molecular Plant2013，6，5： | 14 |
| 2 | Wound Complications in 1145 Consecutive Transumbilical Single-Incision Laparoscopic Procedures显示文摘 | Helmut G. Weiss Walter Brunner Matthias O. Biebl Jan Schirnhofer Katharina Pimpl Christof Mittermair Christian Obrist Eberhard Brunner Tobias Hell | 2014 | Annals of Surgery2014，，1： | 3 |
| 3 | Single port laparoscopy in gastroenterology and hepatology: A fine step forward显示文摘Single incision laparoscopy(SIL) has become an emerging technology aiming at a further reduction of abdominal wall trauma in minimally invasive surgery. Available data is encouraging for the safe application of standardized SIL in a wide range of procedures in gastroenterology and hepatology. Compared to technically simple SIL procedures, the merit of SIL in advanced surgeries, such as liver or colorectal interventions, compared to conventional laparsocopy is self-evident without any doubt. SIL has already passed the learning curve and is routinely utilized in expert centers. This minimized approach has allowed to enter a new era of surgical management that can not be acceded without a fruitful combination of prudent training, consistent day-to-day work and enthusiastic motivation for technical innovations. Both, basic and novel technical specifics as well as particular procedures are described herein. The focus is on the most important surgical interventions in gastroenterology and aims at reviewingthe current literature and shares our experience in a high volume center. | Christof Mittermair Jan Schirnhofer Eberhard Brunner Katharina Pimpl Christian Obrist Michael Weiss Helmut G Weiss | 2014 | World Journal of Gastroenterology2014，20，42： | 3 |
| 4 | A prototype high-resolution animal positron tomograph with avalanche photodiode arrays and LSO crystals显示文摘 | Sibylle I. Ziegler Bernd J. Pichler Guido Boening Magdalena Rafecas Wendelin Pimpl Eckart Lorenz Norbert Schmitz Markus Schwaiger | 2001 | European Journal of Nuclear Medicine and Molecular Imaging2001，，2： | 1 |
| 5 | Isolated tuberculosis of posterior elements of spine:magnetic resonance imaging findings in 33 patients显示文摘 | Narlawar R S Shah J R Pimple MK | 2002 | Spine2002，27，3： | 1 |
| 6 | Development of a new electrodepo-sition method for Pu-determination in environmental samples 显示文摘 | Lee M H Pimpl M | 1999 | Applied Radiation and Isotopes1999，50，： | 1 |
| 7 | Isolated tuberculosis of posterior elements of spine:magnetic resonance imaging findings in 33 patients显示文摘 | Narlawar RS Shah JR Pimple MK | 2002 | Spine2002，27，3： | 1 |
| 8 | Isolated tuberculosis of posterior elements of spine: magnetic resonance imaging findings in 33 patients显示文摘 | Narlawar RS Shah JR Pimple MK | 2002 | Spine2002，27，： | 1 |
| 9 | Effect of a modified stmma resection technic on the rate of lesions of the recurrent laryngeal nerve显示文摘 | Pimpl W Riedl E | 1987 | Chirurg1987，58，： | 1 |
| 10 | Estimation of the mortality rate of patients with severe gastroduodenal hemorrhage with the aid of a new scoring system 显示文摘 | W PIMPL 0 BOECKL WACLAWICZEK HW | 1987 | Endoscopy1987，19，： | 1 |
| 11 | Depressive symptoms are associated with mental stress -induced myocardial is- chemia after acutemyocardial infarction显示文摘 | Wei J Pimple P Shah AJ | 2014 | PLoS One2014，9，10： | 1 |
| 12 | Incidence of septic and throm boembolic related deaths after splenectomy in adults显示文摘 | PIMPL W KAINDL H | 1989 | Br J Surg1989，76，5： | 1 |
| 13 | An anatomic study of the popliteofibular ligament显示文摘 | Wadia FD Pimple M Gajjar SM | 2003 | Int Orthop2003，27，3： | 1 |
| 14 | In situ localization and in vitro induction of plant COPI-coated vesicles显示文摘 | Pimpl P Movafeghi A Coughlan S | 2000 | Plant Cell2000，12，： | 1 |
| 15 | Microscopic evaluation and physicochemical analysis of Origanum majorana Linn leaves显示文摘 | BP Pimple AN Patel PV Kadam MJ Patil | 2012 | Asian Pacific Journal of Tropical Disease2012，，： | 1 |
| 16 | Cytology versus HPV testing for the detection of high-grade cervical lesions in women found positive on visual inspection in Mumbai,India显示文摘 | Pimple S Muwonge R Amin G | 2010 | Int J Gynaecol Obstet2010，108，： | 1 |
| 17 | Vibrational spectra of synthetic crandallite-type minerals-optical and inelastic neutron scattering spectra 显示文摘 | Breitinger D K Brehm G Mohr J Colognesi D Parker S F Stolle A Pimpl Th H Schwab R G | 2006 | Raman Spectrosc2006，37，： | 1 |
| 18 | Management of shoulder instability in a military population 显示文摘 | Pimple MK DaCosta A Clasper JC | 2008 | J R Army Med Corps2008，154，1： | 1 |
| 19 | Cytology versus HPV test- ing for the detection of high-grade cervical lesions in women found positive on visual in spection in Mumbai, India显示文摘 | Pimple S Muwonge R Amin G | 2010 | Int J Gynaecol Obstet2010，108，3： | 1 |
| 20 | Isolated tuberculosis of posterior elements of spine:magnetic resonance imaging findings in 33 patients显示文摘 | Narlawar RS Shah JR Pimple MK | 2002 | Spine2002，27，3： | 1 |
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