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| 1 | SlMYB75,an MYB-type transcription factor,promotes anthocyanin accumulation and enhances volatile aroma production in tomato fruits显示文摘Genetic manipulation of genes to upregulate specific branches of metabolic pathways is a method that is commonly used to improve fruit quality.However,the use of a single gene to impact several metabolic pathways is difficult.Here,we show that overexpression of the single gene SlMYB75(SlMYB75-OE)is effective at improving multiple fruit quality traits.In these engineered fruits,the anthocyanin content reached 1.86mg g−1 fresh weight at the red-ripe stage,and these SlMYB75-OE tomatoes displayed a series of physiological changes,including delayed ripening and increased ethylene production.In addition to anthocyanin,the total contents of phenolics,flavonoids and soluble solids in SlMYB75-OE fruits were enhanced by 2.6,4,and 1.2 times,respectively,compared to those of wild-type(WT)fruits.Interestingly,a number of aroma volatiles,such as aldehyde,phenylpropanoid-derived and terpene volatiles,were significantly increased in SlMYB75-OE fruits,with some terpene volatiles showing more than 10 times higher levels than those in WT fruits.Consistent with the metabolic assessment,transcriptomic profiling indicated that the genes involved in the ethylene signaling,phenylpropanoid and isoprenoid pathways were greatly upregulated in SlMYB75-OE fruits.Yeast one-hybrid and transactivation assays revealed that SlMYB75 is able to directly bind to the MYBPLANT and MYBPZM cis-regulatory elements and to activate the promoters of the LOXC,AADC2 and TPS genes.The identification of SlMYB75 as a key regulator of fruit quality attributes through the transcriptional regulation of downstream genes involved in several metabolic pathways opens new avenues towards engineering fruits with a higher sensory and nutritional quality. | Wei Jian Haohao Cao Shu Yuan Yudong Liu Juanfang Lu Wang Lu Ning Li Jianhui Wang Jian Zou Ning Tang Chan Xu Yulin Cheng Yanqiang Gao Wanpeng Xi Mondher Bouzayen Zhengguo Li | 2019 | Horticulture Research2019，6，1： | 39 |
| 2 | MicroTom Metabolic Network: Rewiring Tomato Metabolic Regulatory Network throughout the Growth Cycle显示文摘Tomato(Solanum lycopersicum)is a major horticultural crop worldwide and has emerged as a preeminent model for metabolic research.Although many research efforts have focused on the analysis of metabolite differences between varieties and species,the dynamics of metabolic changes during the tomato growth cycle and the regulatory networks that underlie these changes are poorly understood.In this study,we integrated high-resolution spatio-temporal metabolome and transcriptome data to systematically explore the metabolic landscape across 20 major tomato tissues and growth stages.In the resulting MicroTom Metabolic Network,the 540 detected metabolites and their co-expressed genes could be divided into 10 distinct clusters based on their biological functions.Using this dataset,we constructed a global map of the major metabolic changes that occur throughout the tomato growth cycle and dissected the underlying regulatory network.In addition to verifying previously well-established regulatory networks for important metabolites,we identified novel transcription factors that regulate the biosynthesis of important secondary metabolites such as steroidal glycoalkaloids and flavonoids.Our findings provide insights into spatiotemporal changes in tomato metabolism and generate a valuable resource for the study of metabolic regulatory processes in model plants. | Yan Li Yang Chen Lu Zhou Shengjie You Heng Deng Ya Chen Saleh Alseekh Yong Yuan Rao Fu Zixin Zhang Dan Su Alisdair R.Fernie Mondher Bouzayen Tao Ma Mingchun Liu Yang Zhang | 2020 | Molecular Plant2020，13，8： | 10 |
| 3 | Exploiting Natural Variation in Tomato to Define Pathway Structure and Metabolic Regulation of Fruit Polyphenolics in the Lycopersicum Complex显示文摘While the structures of plant primary metabolic pathways are generally well defined and highly conserved across species,those defining specialized metabolism are less well characterized and more highly variable across species.In this study,we investigated polyphenolic metabolism in the lycopersicum complex by characterizing the underlying biosynthetic and decorative reactions that constitute the metabolic network of polyphenols across eight different species of tomato.For this purpose,GC-MS-and LC-MS-based metabolomics of different tissues of Solatium lycopersicum and wild tomato species were carried out,in concert with the evaluation of cross-hybridized microarray data for MapMan-based transcriptomic analysis,and publicly available RNA-sequencing data for annotation of biosynthetic genes.The combined data were used to compile species-specific metabolic networks of polyphenolic metabolism,allowing the establishment of an entire pan-species biosynthetic framework as well as annotation of the functions of decoration enzymes involved in the formation of metabolic diversity of the flavonoid pathway.The combined results are discussed in the context of the current understanding of tomato flavonol biosynthesis as well as a global view of metabolic shifts during fruit ripening.Our results provide an example as to how large-scale biology approaches can be used for the definition and refinement of large specialized metabolism pathways. | Takayuki Tohge Federico Scossa Regina Wendenburg Pierre Frasse llse Balbo Mutsumi Watanabe Saleh Alseekh Sagar Sudam Jadhav Jay C.Delfin Marc Lohse Patrick Giavalisco Bjoern Usadel Youjun Zhang Jie Luo Mondher Bouzayen Alisdair R.Fernie | 2020 | Molecular Plant2020，13，7： | 3 |
| 4 | 盐酸美金刚在中国治疗阿尔茨海默病的药物经济学模型显示文摘目的探讨从中度阿尔茨海默病(AD)开始盐酸美金刚治疗与仅从重度AD开始盐酸美金刚治疗在中国的临床和经济效益。方法运用马尔可夫模型,通过定义患者的不同健康状况,模拟了中度AD患者在5年中的疾病进展。研究对两组患者进行了比较:1从中度AD开始接受美金刚治疗(进入模型的时期)并持续至病情发展到重度AD的患者;2仅从重度AD开始接受美金刚治疗的患者。结果 5年后,从中度AD开始美金刚治疗的患者中,较少的患者发展至重度AD(49%),依赖他人(59%)或有激越行为(47%);相比对于从重度AD开始治疗的患者分别为58%、67%和55%。从中度AD开始美金刚治疗的全国总治疗费用(670亿人民币)低于从重度AD开始的费用(730亿人民币)。结论在中国,从中度AD开始使用美金刚治疗可以节省大量成本。 | 胡善联 于欣 Emilie Clay Mondher Toumi Dominique Milea | 2016 | 中国药物经济学2016，0，9： | 2 |
| 5 | Clinical management and associated costs for moderate and severe Alzheimer’s disease in urban China: a Delphi panel study显示文摘Background:Healthcare resource utilisation for Alzheimer’s disease(AD)in China is not well understood.This Delphi panel study aimed to describe the clinical management pathways for moderate and severe AD patients in urban China and to define the amount and cost of healthcare resources used.Methods:A panel of 11 experts was recruited from urban China to participate in two rounds of preparatory interviews.In the first round,9 physicians specialised in dementia gave a qualitative description of the clinical management of AD patients.In the second round,2 hospital administrators were asked about the cost of AD management and care.Results from the interviews were discussed by the experts in a Delphi panel meeting,where consensus was reached on quantitative aspects of AD management,including the rate of healthcare resource utilisation,the respective unit costs and caregiving time.Results:Interviewees reported that mild AD is under-recognised in China;most patients are diagnosed with moderate to severe AD.Loss of independence and agitation/aggression are the main drivers for healthcare resource utilisation and contribute to a heavier caregiver burden.It was estimated that 70%moderate AD patients are independent/non-aggressive at the time of diagnosis,15%are independent/aggressive,10%are dependent/non-aggressive,and 5%are dependent/aggressive.Dependent/aggressive AD patients are more likely to be hospitalised(70–90%)than accepted in a nursing home(0–20%),while the opposite is true for dependent/non-aggressive patients(5–35%for hospitalisation vs.80%for nursing home).Independent AD patients require 1–3 hours/day of caregiver time,while dependent patients can require up to 12–15 hours/day.Experts agreed that AD complicates the management of age-related comorbidities,found in 70–80%of all AD patients,increasing the frequency and cost of hospitalisation.Conclusions:The Delphi panel approach was an efficient method of gathering data about the amount of healthcare resources used and associated costs for moderate and severe AD patients in urban China.The results of this study provide a useful source of information for decision makers to improve future healthcare policies and resource planning,as well as to perform economic evaluations of AD therapies. | Xin Yu Shengdi Chen Xiaochun Chen Jianjun Jia Chunhou Li Cong Liu Mondher Toumi Dominique Milea | 2015 | Translational Neurodegeneration2015，4，1： | 2 |
| 6 | Activity assessment of Tunisian olive leaf extracts against the trophozoite stage of Acanthamoeba显示文摘 | Ines Sifaoui Atteneri López-Arencibia Carmen Ma Martín-Navarro Nadia Chammem Mondher Mejri Jacob Lorenzo-Morales Manef Abderabba José E. Pi?ero | 2013 | Parasitology Research2013，，8： | 1 |
| 7 | Threshold Effects in the Capital Account Liberalization and Foreign Direct Investment Relationship显示文摘 | Gammoudi Mouna Cherif Mondher | 2014 | Chinese Business Review2014，13，9： | 1 |
| 8 | Effeets of some additives on wheat gluten solubility: A structural approach 显示文摘 | Mondher Mejri Barbara Roge Abdelfattah BenSouissi Franck Michels Mohamed Mathlouthi | 2005 | Food Chemistry2005，92，： | 1 |
| 9 | Coastal tourism and 'dutch disease' in a small island economy显示文摘 | Jean-Jacques Nowak & Mondher Sahli | 2007 | Tourism Eco nomics2007，13，1： | 1 |
| 10 | 1-aminocyclopropane-1-carboxylate oxidase of apple fruit is periplasmic显示文摘 | Sabine Ramassamy Enrique Olmos Mondher Bouzayen | 1998 | Journal of Experimental Botany1998，49，： | 1 |
| 11 | Evaluation of antioxidant activities of the edible and medicinal Suaeda species and related phenolic compounds显示文摘 | Samia Oueslati Najla Trabelsi Mondher Boulaaba | 2012 | Industrial Crops and Products2012，36，： | 1 |
| 12 | Geranylgeranyi diphosphate synthase activity and taxane production in Taxus baccata cells显示文摘 | Gregory L Camiel F D Mondher J | 1999 | Phytochemistry1999，50，1： | 1 |
| 13 | The chimeric repressor version of an E thylene Response Factor ( ERF ) family member, Sl‐ ERF .B3 , shows contrasting effects on tomato fruit ripening显示文摘 | Mingchun Liu Gianfranco Diretto Julien Pirrello Jean‐Paul Roustan Zhengguo Li Giovanni Giuliano Farid Regad Mondher Bouzayen | 2014 | New Phytol2014，，1： | 1 |
| 14 | Effect of annealing on photoluminescence and optical properties of porous anodic alumina films formed in sulfuric acid for solar energy applications显示文摘 | Mondher Ghrib Rachid Ouertani Monir Gaidi Najoua Khedher Mohamed Ben Salem Hatem Ezzaouia | 2011 | Applied Surface Science2011，，12： | 1 |
| 15 | 病毒诱导的基因沉默在番茄组织中的特异性分析显示文摘病毒诱导的基因沉默(VIGS)是植物基因功能研究的新技术。以组成型表达gusA基因的转基因番茄为材料,通过VIGS技术沉默gusA基因,对VIGS在番茄中的组织特异性进行了初步分析。结果表明,VIGS技术能有效地诱导gusA基因在番茄植株的叶片、花、果实、侧枝、主茎及茎分枝点等部位和组织中沉默,证明VIGS在番茄中具有较强的系统性沉默特征。 | 杨迎伍 李正国 Mondher Bouzayen | 2008 | 园艺学报2008，35，2： | 1 |
| 16 | Effect of chemical modification of luffa cylindrical fibers on the mechanical and hygrothermal behaviours of polyester/luf fa composites显示文摘 | Lassaad Ghali Mourad Aloui Mondher Zidi etal | 2011 | Bioresources2011，6，4： | 1 |
| 17 | Nitrided zeolites:A spectroscopic approach for the identification and quantification of incorporated nireogen species显示文摘 | Mondher S Stephanie D Eric M G | | 0，，10： | 1 |
| 18 | Effects of some additives on wheat gluten solubility: A structural approach显示文摘 | Mondher Mejri Barbara Rogé Abdelfattah BenSouissi Franck Michels Mohamed Mathlouthi | 2004 | Food Chemistry2004，，1： | 1 |
| 19 | Two highly divergent alcohol dehydrogenases of melon exhibit fruit ripening-specific expression and distinct biochemical characteristics显示文摘 | Daniel Manríquez Islam El-Sharkawy Francisco B. Flores Fikri El-Yahyaoui Farid Regad Mondher Bouzayen Alain Latché Jean-Claude Pech | 2006 | Plant Molecular Biology (-)2006，，4： | 1 |
| 20 | Overexpression of StDREB1 Transcription Factor Increases Tolerance to Salt in Transgenic Potato Plants显示文摘 | Donia Bouaziz Julien Pirrello Mariam Charfeddine Asma Hammami Rania Jbir Amina Dhieb Mondher Bouzayen Radhia Gargouri-Bouzid | 2013 | Molecular Biotechnology2013，，3： | 1 |
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