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| 1 | Establishment of NOD/SCID mouse models of human hepatocellular carcinoma via subcutaneous transplantation of histologically intact tumor tissue显示文摘Hepatocellular carcinoma (HCC) is one of the most deadly human cancers, but it is very difficult to establish an animal model by using surgical specimens. In the present experiment, histologically intact fresh surgical specimens of HCC were subcutaneously transplanted in non-obese diabetic/severe combined immunodeficienccy (NOD/SCID) mice. The biological characteristics of the original and the corresponding transplanted tumors and cell lines were investigated. The results showed that 5 new animal models and 2 primary cell lines were successfully established from surgical specimens. Hematoxylin-eosin staining showed that xenografts retained major histological features of the original surgical specimens. The two new cell lines had been cultivated for 3 years and successively passaged for more than 100 passages in vitro. The morphological characteristics and biologic features of the two cell lines were genetically similar to the original tumor. The subcutaneous transplant animal models with histologically intact tumor tissue and primary cell lines could be useful for in vivo and in vitro testing of anti-cancer drugs and be ideal models to study various biologic features of HCC. | Mingxia Yan Hong Li Fangyu Zhao Lixing Zhang Chao Ge Ming Yao Jinjun Li | 2013 | Chinese Journal of Cancer Research2013，25，3： | 2 |
| 2 | 显示文摘 | Lin Yan Gao Mingxia Li Yufeng | 2008 | The chinese Journal of Nonferrous Metals2008，18，3： | 1 |
| 3 | Functional reorganization associated with outcome in hand function after stroke revealed by regional homogeneity显示文摘 | Dazhi Yin Yanli Luo Fan Song Dongrong Xu Bradley S. Peterson Limin Sun Weiwei Men Xu Yan Mingxia Fan | 2013 | Neuroradiology2013，，6： | 1 |
| 4 | Developing a strong anion exchange/RP (SAX/RP) 2D LC system for high‐abundance proteins depletion in human plasma显示文摘 | Shaochun Zhu Xueyang Zhang Mingxia Gao Guangfeng Hong Guoquan Yan Xiangmin Zhang | 2012 | Proteomics (-)2012，，23： | 1 |
| 5 | Correlation of Ferroptosis and Other Types of Cell Death in Neurodegenerative Diseases显示文摘Ferroptosis is defined as an iron-dependent,non-apoptotic cell death pathway,with specific morphological phenotypes and biochemical changes.There is a growing realization that ferroptosis has significant implications for several neurodegenerative diseases.Even though ferroptosis is different from other forms of programmed death such as apoptosis and autophagic death,they involve a number of common protein molecules.This review focuses on current research on ferroptosis and summarizes the cross-talk among ferroptosis,apoptosis,and autophagy that are implicated in neurodegenerative diseases.We hope that this information provides new ideas for understanding the mechanisms and searching for potential therapeutic approaches and prevention of neurodegenerative diseases. | Xiaoting Dang Xuejie Huan Xixun Du Xi Chen Mingxia Bi Chunling Yan Qian Jiao Hong Jiang | 2022 | Neuroscience Bulletin2022，38，8： | 1 |
| 6 | Epidemiological survey and genetic characterization of type 3 vaccine-derived poliovirus isolated from a patient with four doses of inactivated polio vaccine in Henan Province,China显示文摘Background:Vaccine-derived poliovirus(VDPV)is a potential threat to polio eradication because they can reintroduce into the general population and cause paralytic polio outbreaks,a phenomenon that has recently emerged as a prominent public health concern at the end of global polio eradication.This study aimed to describe the epidemiology and genetic characteristics of the frst VDPV identifed from a patient with acute faccid paralysis(AFP),with four doses of inactivated polio vaccine immunization in Henan Province,China in 2017.Methods:The patient was diagnosed with type 3 VDPV.Subsequently,a series of epidemiological approaches was implemented,including a retrospective search of AFP cases,rate of vaccination assessment,study of contacts,and supplementary immunization activities.Fecal samples were collected,viral isolation was performed,and the viral isolates were characterized using full-length genomic sequencing and bioinformatic analysis.Results:Phylogenetic analysis showed that the viral isolates from the patient were diferent from other reported genetic clusters of type 3 VDPV worldwide.They were identifed as a Sabin 3/Sabin 1 recombinant VDPV with a crossover site in the P2 region.Nucleotide substitutions,including U→C(472)and C→U(2493),have been identifed,both of which are frequently observed as reversion mutations in neurovirulent type 3 poliovirus.A unique aspect of this case is that the patient had been vaccinated with four doses of inactive polio vaccine,and the serum neutralizing antibody for Sabin types 1 and 3 were 1∶16 and 1∶512,respectively.Thus,the patient was speculated to have been infected with type 3 VDPV,and the virus continued to replicate and be excreted for at least 41 d.Conclusions:The existence of this kind of virus in human population is a serious risk and poses a severe challenge in maintaining a polio-free status in China.To the best of our knowledge,this is the frst report of VDPV identifed in the Henan province of China.Our results highlight the importance of maintaining a high-level vaccination rate and highly sensitive AFP case surveillance system in intercepting VDPV transmission. | Mingyu Zhang Jianhui Yang Yiran Bai Hui Zhu Changshuang Wang Lu Zhang Jin Xu Mingxia Lu Xiaoxiao Zhang Zhanpei Xiao Yating Ma Yan Wang Xiaolei Li Dongyan Wang Shuangli Zhu Dongmei Yan Wenbo Xu Yong Zhang Yanyang Zhang | 2022 | Infectious Diseases of Poverty2022，11，6： | 0 |
| 7 | Two-dimensional silica enhanced solid polymer electrolyte for lithium metal batteries显示文摘Solid polymer electrolytes(SPEs)are promising substitutes for current flammable liquid electrolytes to achieve high-safety and high-energy-density lithium metal batteries.Polyethylene oxide(PEO)based solid polymer electrolytes have attracted increasing attention because of their excellent flexibility,manufacturability,light weight,and low-cost processing,while they often suffer from low ionic con-ductivity at room temperature,low lithium transference number and unsatisfactory interfacial resis-tance,which largely restrain their practical application.Herein,two-dimensional holey silica nanosheets(2D-HSN)as the fillers,together with LiNO_(3) as the electrolyte additive,are introduced in a PEO/poly(-vinylidene fiuoride-co-hexafluoropropylene)(PVDF-HFP)blended polymer matrix to obtain a SPE.The incorporation of HSN filler creates supplementary channels for lithium ion migration and lowers the crystallinity of the polymer,thereby facilitating the movement of lithium ions.The HSN-based SPE demonstrates higher ionic conductivity up to 3.7 x 10-4 S cm-1 at 30℃,larger Li+transference number close to 0.34,and more stable lithium plating/stripping than that without the fillers,and HSN can promote the formation of more stable solid electrolyte interphase(SEI)layer.The as-assembled LiFePO4||Li batteries deliver a high specific capacity of 159 mA h g-1 with the capacity retention of 95.5%after 200 cycles at 30℃,as well as superior rate performance and cycling stability compared to that using the blank SPE. | Zhihong Luo Weiyong Li Chong Guo Ya Song Mingxia Zhou Yan Shi Jing Xu Long Li Bin Shi Quansheng Ouyang Jiao-jing Shao Guangmin Zhou | 2024 | Particuology2024，85，2： | 0 |
| 8 | Clinical Study of Association of Hs-CRP with Obesity and Diabetes显示文摘 | Mingxia Sun Liying Zhang Shanying Chen Xinyu Liu Yan Liang Xiaofei Shao Hequn Zou | 2014 | 器官移植内科学杂志2014，9，4： | 0 |
| 9 | Regulatory Effects of Zuogui Pill on Apoptosis of Follicles in Rats Injured by 60Co-γRays Based on PI3K/Akt/m TOR Signaling Pathway显示文摘[Objectives]To explore the protective effects of Zuogui Pill on ^(60)Co-γ-ray-induced premature aging of rats based on phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR)signaling pathway.[Methods]Sixty sexually mature female SD rats were irradiated with ^(60)Co-γ-ray(6.0 Gy,LD 40)for 24 h at one time.These rats were randomly divided into model group,Progynova group[0.18(g·kg)/d],Progynova[0.09(g·kg)/d]+Zuogui Pill high dose[23.625(g·kg)/d)]group,Zuogui Pill high dose[23.625(g·kg)/d)]group,Zuogui Pill medium dose[9.45(g·kg)/d)]group and Zuogui Pill low dose[4.725(g·kg)/d]group.The administration(once a day)lasted 21 d.The rat serum[follicle-stimulating hormone(FSH),luteinizing hormone(LH)and estradiol(E_(2))]were detected by Enzyme-linked immunosorbent assay(ELISA).The morphological changes of ovary were observed by hematoxylin-eosin(HE)staining.The apoptosis rate of granulosa cells was detected by terminal deoxynucleotidyl transferase(TdT)-mediated dUTP nick-end labeling(TUNEL).The protein expression of phosphorylated(p)-PI3K,p-Akt,p-mTOR,B-cell lymphoma-2(Bcl-2),and Bcl-2-associated X protein(Bax)in ovarian tissues were detected by Western blot.[Results]Compared with the normal group,the model group showed significant increase in the serum FSH(P<0.01),significant decrease in serum E_(2)(P<0.05),and decrease in the number of early follicles and luteum in the ovary(P<0.01).Besides,the apoptosis rate of granulosa cells increased significantly(P<0.01);the expression of p-PI3K,p-Akt,p-mTOR and Bcl-2 in ovarian tissue decreased significantly,while the expression of Bax increased significantly(P<0.01).Compared with the model group,the number of early follicles in the ovary increased and the apoptosis rate of granulosa cells decreased after intervention in each administration group.In addition,the protein expressions of p-PI3K,p-Akt,p-mTOR and Bcl-2 increased,while the expression of Bax decreased,especially in Progynova+Zuogui Pill high dose group,the differences were statistically significant(P<0.05,P<0.01).[Conclusions]Zuogui Pill may protect the radiation-injured ovary through activating the expression of PI3K/Akt/mTOR protein in ovarian tissue,increasing the amount of Bcl-2 protein and inhibiting the expression of Bax protein. | Fenqin ZHAO Mingxia AN Xiaonan DING Jieying LIU Yan ZHAO Zhihui XIE Shuping LI | 2022 | Medicinal Plant2022，13，5： | 0 |
| 10 | Tuning coordination environment of iron ions to ensure ultra-high pseudocapacitive capability in iron oxide显示文摘The mechanism governing the pseudocapacitive lithium storage behavior is one of the most critical unsolved issues in conversion-type anodes for lithium-ion batteries.In this work,we,for the first time,demonstrate that the pseudocapacitive capability of iron oxide-based anodes is closely associated with the electronic structures of iron ions.As proof of concept,the introduction of amorphization,nitrogen doping,oxygen vacancies reduces the coordination of iron ions and contributes significantly to the pseudocapacitive lithium storage capability of iron oxide,reaching up to 96%of the specific capacity at 1 mV·s^(−1).Due to the significantly modulated coordination environment,the 3d electrons of Fe(II)are delocalized with increased spin state and the energy band gap is narrowed,accompanied by an upshift of Fermi energy.The redox activity and carrier mobility of iron oxides are substantially increased,which substantially enhance the exchange current density and thereby improve the pseudocapacitive capability of iron oxide. | Wubin Du Chenhui Yan Mingxi Gao Jian Chen Panyu Gao Xuebin Yu Yinzhu Jiang Wenping Sun Yongfeng Liu Mingxia Gao Shibo Xi Hongge Pan | 2023 | Nano Research2023，16，5： | 0 |
| 11 | Application of a Soil Nutrient Measuring Instrument in Vegetable Seedling Substrate显示文摘[Objective] The aim was to find an efficient method to measure available nitrogen,phosphorus and potassium content in vegetable seedling substrate.[Method] The suitable dosage of leaching agent,ratio of the substrate to water and leaching time for the detection of nutrient contents in the substrate by a soil nutrient measuring instrument were discussed firstly,and then the results of nutrient contents measured by the soil nutrient measuring instrument were compared with that by conventional approaches.[Result]In comparison with the conventional methods,the average content of available nitrogen measured by the soil nutrient measuring instrument was slightly higher,while the average content of available phosphorus measured by the instrument was lower; the average content of available potassium measured by the instrument was close to that by the conventional method.[Conclusion] This study could provide us a new method to detect available nitrogen,phosphorus and potassium content in seedling substrate efficiently. | Wang Pengcheng Zhang Jian Tian Hongmei Jiang Haikun Wang Yan Wang Mingxia Fang Ling | 2015 | Meteorological and Environmental Research2015，6，11： | 0 |
| 12 | Clinical features of 162 fatal cases of COVID-19:a multi-center retrospective study显示文摘Background:The coronavirus disease 2019(COVID-19)has affected approximately 2 million individuals worldwide;however,data regarding fatal cases have been limited.Objective:To report the clinical features of 162 fatal cases of COVID-19 from 5 hospitals in Wuhan between December 30,2019 and March 12,2020.Methods:The demographic data,signs and symptoms,clinical course,comorbidities,laboratory findings,computed tomographic(CT)scans,treatments,and complications of the patients with fatal cases were retrieved from electronic medical records.Results:The median patient age was 69.5(interquartile range:63.0–77.25)years,and 80%of the patients were over 61 years.A total of 112(69.1%)patients were men.Hypertension(45.1%)was the most common comorbidity,while 59(36.4%)patients had no comorbidity.At admission,131(81.9%)patients had severe or critical COVID-19,whereas 39(18.1%)patients with hypertension or chronic lung disease had moderate COVID-19.In total,126(77.8%)patients received antiviral treatment,while 132(81.5%)patients received glucocorticoid treatment.A total of 116(71.6%)patients were admitted to the intensive care unit(ICU),and 137(85.1%)patients received mechanical ventilation.Most patients received mechanical ventilation before ICU admission.Approximately 93.2%of the patients developed respiratory failure or acute respiratory distress syndrome.There were no significant differences in the inhospital survival time among the hospitals(P=0.14).Conclusion:Young patients with moderate COVID-19 without comorbidity at admission could also develop fatal outcomes.The in-hospital survival time of the fatal cases was similar among the hospitals of different levels in Wuhan. | Xianlong Zhou Guoyong Ding Qing Fang Jun Guo Luyu Yang Ping Wang Shou-Zhi Fu Ang Li Jian Xia Jiangtao Yu Jianyou Xia Min Ma Zhuanzhuan Hu Lei Huang Ruining Liu Cheng Jiang Shaoping Li Mingxia Yu Xizhu Xu Yan Zhao Quan Hu Weijia Xing Zhigang Zhao | 2022 | Emergency and Critical Care Medicine2022，2，3： | 0 |
| 13 | Concept and application of anaerobic suspended granular sludge bed (SGSB) reactor for wastewater treatment显示文摘床扩大在 upflow 的设计和操作服务重要功能厌氧的反应堆。扩展小粒的污泥床(EGSB ) 的流动模式的分析大多数 EGSB 反应堆不表现作为的反应堆表演扩展了床反应堆,,广泛地被察觉。更确切地说,这些反应堆基于经典化学反应堆理论作为使流体化的床反应堆表现。在这份报纸,四个床扩大模式,划分的同样静态的床,扩展的床,推迟的床,和使流体化的床,为生物反应器被建议。一个高率的厌氧的推迟的小粒的污泥床(SGSB ) 反应堆然后被开发。SGSB 反应堆是 upflow 厌氧的反应堆,和它的扩大度能容易在一个范围以内被控制由控制 upflow 维持污泥床的推迟的地位速度。照原尺寸的反应堆的结果证实 SGSB 反应堆的使用是有益的。照原尺寸的 SGSB 反应堆稳定地跑并且在高装载率完成高货到付款移动效率(大约 90%)( 一般水准 40 kg | Mingxia ZHENG Zhong YAN Jiane ZUO Kaijun WANG | 2014 | Frontiers of Environmental Science & Engineering2014，8，5： | 0 |
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