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| 1 | Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1显示文摘Ferroptosis is a non-apoptotic regulated cell death caused by iron accumulation and subsequent lipid peroxidation.Currently,the therapeutic role of ferroptosis on cancer is gaining increasing interest.Baicalin an active component in Scutellaria baicalensis Georgi with anticancer potential various cancer types;however,the effects of baicalein on bladder cancer and the underlying molecular mechanisms remain largely unknown.In the study,we investigated the effect of baicalin on bladder cancer cells5637 and KU-19-19.As a result,we show baicalin exerted its anticancer activity by inducing apoptosis and cell death in bladder cancer cells.Subsequently,we for the first time demonstrate baicalin-induced ferroptotic cell death in vitro and in vivo,accompanied by reactive oxygen species(ROS) accumulation and intracellular chelate iron enrichment.The ferroptosis inhibitor deferoxamine but not necrostatin-1,chloroquine(CQ),N-acetyl-L-cysteine,L-glutathione reduced,or carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone(Z-VAD-FMK) rescued baicalin-induced cell death,indicating ferroptosis contributed to baicalin-induced cell death.Mechanistically,we show that ferritin heavy chain1(FTH1) was a key determinant for baicalin-induced ferroptosis.Overexpression of FTH1 abrogated the anticancer effects of baicalin in both 5637 and KU19-19 cells.Taken together,our data for the first time suggest that the natural product baicalin exerts its anticancer activity by inducing FTH1-dependent ferroptosis,which will hopefully provide a prospective compound for bladder cancer treatment. | Na Kong Xiaying Chen Jiao Feng Ting Duan Shuiping Liu Xueni Sun Peng Chen Ting Pan Lili Yan Ting Jin Yu Xiang Quan Gao Chengyong Wen Weirui Ma Wencheng Liu Mingming Zhang Zuyi Yang Wengang Wang Ruonan Zhang Bi Chen Tian Xie Xinbing Sui Wei Tao | 2021 | Acta Pharmaceutica Sinica B2021，11，12： | 23 |
| 2 | Hydrodynamic effect on the superlubricity of phosphoric acid between ceramic and sapphire显示文摘In this work,a super-low friction coefficient of 0.003 was found between a silicon nitride ball and a sapphire plate lubricated by phosphoric acid solution.The wear mainly occurred in the running-in period and disappeared after superlubricity was achieved.The friction coefficient was effectively reduced from 0.3 to 0.003 at a constant speed of 0.076 m/s,accompanied by a 12-nm-thickness film.The lubrication regime was indicated to change from boundary lubrication in the running-in period to elastohydrodynamic lubrication in the superlubricity period,which is also supported by the results of the friction coefficient versus sliding speed.In addition,the experimental results showed good agreement with theoretical calculations based on the elastohydrodynamic lubrication theory,suggesting a significant hydrodynamic effect of phosphoric acid on superlubricity. | Mingming DENG Chenhui ZHANG Jinjin LI Liran MA Jianbin LUO | 2014 | Friction2014，2，2： | 11 |
| 3 | Self-supported high-entropy alloy electrocatalyst for highly efficient H_(2) evolution in acid condition显示文摘Developing non-precious catalysts as Pt substitutes for electrochemical hydrogen evolution reaction(HER)with superior stability in acidic electrolyte is of critical importance for large-scale,low-cost hydrogen production from water.Herein,we report a CoCrFeNiAl high-entropy alloy(HEA)electrocatalyst with self-supported structure synthesized by mechanical alloying and spark plasma sintering(SPS)consolidation.The HEA after HF treatment and in situ electrochemical activation for 4000 cycles of cyclic voltammetry(HF-HEAa2)presents favourable activity with overpotential of 73 mV to reach a current density of 10 mA cm^(2) and a Tafel slope of 39.7 mV dec1.The alloy effect of Al/Cr with Co/Fe/Ni at atomic level,high-temperature crystallization,as well as consolidation by SPS endow CoCrFeNiAl HEA with high stability in 0.5 M H2SO4 solution.The superior performance of HF-HEAa2 is related with the presence of metal hydroxides/oxides groups on HEA. | Peiyan Ma Mingming Zhao Long Zhang Heng Wang Junfeng Gu Yuchen Sun Wei Ji Zhengyi Fu | 2020 | Journal of Materiomics2020，6，4： | 8 |
| 4 | Curcumenol triggered ferroptosis in lung cancer cells via lncRNA H19/miR-19b-3p/FTH1 axis显示文摘Curcumenol,an effective ingredient of Wenyujin,has been reported that exerted its antitumor potential in a few cancer types.However,the effect and molecular mechanism of curcumenol in lung cancer are largely unknown.Here,we found that curcumenol induced cell death and suppressed cell proliferation in lung cancer cells.Next,we demonstrated that ferroptosis was the predominant method that contributed to curcumenol-induced cell death of lung cancer in vitro and vivo for the first time.Subsequently,using RNA sequencing,we found that the long non-coding RNA H19(lncRNA H19)was significantly downregulated in lung cancer cells treated with curcumenol,when compared to untreated controls.Overexpression of lncRNA H19 eliminated the anticancer effect of curcumenol,while lncRNA H19 knockdown promoted ferroptosis induced by curcumenol treatment.Mechanistically,we showed that lncRNA H19 functioned as a competing endogenous RNA to bind to miR-19b-3p,thereby enhanced the transcription activity of its endogenous target,ferritin heavy chain 1(FTH1),a marker of ferroptosis.In conclusion,our data show that the natural product curcumenol exerted its antitumor effects on lung cancer by triggering ferroptosis,and the lncRNA H19/miR-19b-3p/FTH1 axis plays an essential role in curcumenol-induced ferroptotic cell death.Therefore,our findings will hopefully provide a valuable drug for treating lung cancer patients. | Ruonan Zhang Ting Pan Yu Xiang Mingming Zhang Han Xie Zimao Liang Bi Chen Cong Xu Jing Wang Xingxing Huang Qianru Zhu Ziming Zhao Quan Gao Chengyong Wen Wencheng Liu Weirui Ma Jiao Feng Xueni Sun Ting Duan Elaine Lai-Han Leung Tian Xie Qibiao Wu Xinbing Sui | 2022 | Bioactive Materials2022，7，7： | 8 |
| 5 | The magnetic properties of Serbian loess and its environmental significance显示文摘This paper reports a loess-paleosol sequence located in the Danube River basin,Serbia,which formed at least since the latest part of the early Pleistocene,and before the paleomagnetic B/M boundary.Various magnetic parameters of the Serbian V-L1-V-S4 loess-paleosol sequence have been measured and analyzed in the Titel Loess Plateau.These parameters show a very similar magnetic behavior compared with that of the Chinese loess.There is a general positive relationship between magnetic susceptibility() and pedogenesis.The main contributors to are the magnetic grains of SP(superparamagnetic) and SD(single domain) magnetic domains,while MD(multi domain) contributes only a small percentage.The difference in between loess and paleosol mainly is caused by pedogenesis.The very fine magnetic minerals increase gradually with increasing soil development(from loess to soil),and they lead to higher.The thermomagnetic curves show thatmagnetic minerals in the loess layers are magnetite and maghemite,both providing a major contribution to.In contrast the paleosol layers mainly are composed of magnetite,with almost no or a very small amount of maghemite,as implied by a reversible thermomagnetic behavior.This indicates that pedogenic conditions during V-S3 and V-S4 strong soil development have resulted in maghemite that is no longer stable,and has been resolved or converted to other stable phase minerals.This likely indicates that soil moisture during V-S3 and V-S4 development exceeded a critical condition of maghemite stability. | LIU XiuMing LIU Zhi Lü Bin MARKOVI S B CHEN JiaSheng GUO Hui MA MingMing ZHAO GuoYong FENG Hua | 2013 | Chinese Science Bulletin2013，58，3： | 7 |
| 6 | Electrosynthesis of Co3O4 and Co(OH)2 ultrathin nanosheet arrays for efficient electrocatalytic water splitting in alkaline and neutral media显示文摘 | Lin Zhang Bingrui Liu Ning Zhang Mingming Ma | 2018 | Nano Research2018，11，1： | 6 |
| 7 | Radiation-pressure-driven mechanical oscillations in silica microdisk resonators on chip显示文摘We demonstrate radiation-pressure-driven mechanical oscillations from high optical quality factor silica microdisk resonators on chip. Mechanical quality factors of 3520 in air and 12540 in vacuum for the fundamental radial breathing modes are obtained from 73 μm-diameter silica microdisks with mechanical frequencies of ~51 MHz. The measured mechanical oscillation threshold powers for the input light are determined to be 62.5 μW in air and down to 26.6 μW in vacuum. | WANG GuanZhong ZHAO MingMing MA JiYang LI GuanYu CHEN Yuan JIANG XiaoShun XIAO Min | 2015 | Science China(Physics,Mechanics & Astronomy)2015，58，5： | 6 |
| 8 | Dent Resistance for Automobile Body Panels显示文摘Dent resistance of automobile body panels is an important property for automobile design and manufacture, but the study on this aspect is not still profound. This study is to summarize the testing methods and physical significations of static and dynamic dent resistance of automobile body panels combined with the author's study, and to analyze the dent behaviors in the round. Several influence factors on dent resistance are expatiated including the mechanical properties of materials, stress states after forming, bake hardening ability, modulus, methods of testing, and structure of specimens and so on. The automotive lightweight and application of high strength steel sheets and aluminum alloys sheets are analyzed, and the significance of testing of dent resistance, especially for dynamic dent resistance of auto-panels, and the finite element simulation analysis are emphasized. To explain the physical phenomenon of dent behaviors, the latest and concerned study results are also discussed. According to this study, a dent resistance test and evaluation standard of Society of Automotive Engineers of China for automotive body panel is presented and is being carried out, and an industry conference is hold to discuss the working-out of the standard, a primary schedule of this standard is confirmed now. The study can guide the further testing and study of dent resistant of auto-panels. | LU Hongzhou MA Mingm YOU Jianghai LI Zhigang | 2009 | Chinese Journal of Mechanical Engineering2009，22，6： | 5 |
| 9 | Meta analysis of olfactory ensheathing cell transplantation promoting functional recovery of motor nerves in rats with complete spinal cord transection显示文摘OBJECTIVE:To evaluate the effects of olfactory ensheathing cell transplantation on functional recovery of rats with complete spinal cord transection.DATA SOURCES: A computer-based online search of Medline(1989–2013),Embase(1989–2013),Cochrane library(1989–2013),Chinese Biomedical Literature Database(1989–2013),China National Knowledge Infrastructure(1989–2013),VIP(1989–2013),Wanfang databases(1989–2013) and Chinese Clinical Trial Register was conducted to collect randomized controlled trial data regarding olfactory ensheathing cell transplantation for the treatment of complete spinal cord transection in rats.SELECTION CRITERIA: Randomized controlled trials investigating olfactory ensheathing cell transplantation and other transplantation methods for promoting neurological functional recovery of rats with complete spinal cord transection were included in the analysis.Meta analysis was conducted using Rev Man 4.2.2 software.MAIN OUTCOME MEASURES: Basso,Beattie and Bresnahan scores of rats with complete spinal cord transection were evaluated in this study.RESULTS: Six randomized controlled trials with high quality methodology were included.Meta analysis showed that Basso,Beattie and Bresnahan scores were significantly higher in the olfactory ensheathing cell transplantation group compared with the control group(WMD = 3.16,95% CI(1.68,4.65); P < 0.00001).CONCLUSION: Experimental studies have shown that olfactory ensheathing cell transplantation can promote the functional recovery of motor nerves in rats with complete spinal cord transection. | Jun Liu Ping Chen Qi Wang Yu Chen Hailong Yu Junxiong Ma Mingming Guo Meihui Piao Weijian Ren Liangbi Xiang | 2014 | Neural Regeneration Research2014，9，20： | 4 |
| 10 | Evaluation of the four potential Cretaceous-Paleogene (K-Pg) boundaries in the Nanxiong Basin based on evidences from volcanic activity and paleoclimatic evolution显示文摘Determining the location of the Cretaceous-Paleogene(K-Pg) boundary in terrestrial strata is highly significant for studying the evolution of terrestrial ecosystems at the end of the Cretaceous(especially the extinction of non-avian dinosaurs). At present, research on terrestrial K-Pg boundaries worldwide is concentrated in the middle and high latitudes, such as North America and Northeast China. Although many studies have also been carried out in the Nanxiong Basin, located at low latitudes(which has become the standard for dividing and comparing the continental K-Pg stratigraphy in China), many researchers have proposed four possible boundaries from different perspectives. Therefore, the exact location remains to be determined. In this study, the total mercury(Hg) content, environmental magnetism, geochemistry, and other parameters for the samples collected near the four boundaries were determined and compared with existing records. Results indicated that: 1) The total Hg content significantly increased in the upper part of the Zhenshui Formation and Pingling part of the Shanghu Formation with sharp fluctuations. As per latest dating results of Deccan Traps, the significantly high Hg value was attributed to the Deccan Traps eruption. Boundary 1 was located in the middle of the Hg anomaly interval, which was consistent with the relationship between the global K-Pg boundary and time of volcanic eruption. 2) The reconstructed paleoclimate evolution curve revealed that the red sediments in the basin recorded the late Maastrichtian warming event(66.2 Ma). Regarding the relationship between the four boundaries and this warming event, only boundary 1 was found to be closest to the real K-Pg boundary of the Nanxiong Basin. | Mengting ZHAO Mingming MA Mei HE Yudan QIU Xiuming LIU | 2021 | Science China Earth Sciences2021，64，4： | 4 |
| 11 | Qualitatively and quantitatively assessing the aggregation ability of sludge during aerobic granulation process combined XDLVO theory with physicochemical properties显示文摘Inexact mechanism of aerobic granulation still impedes optimization and application of aerobic granules. In this study, the extended Derjaguin, Landau, Verwey, and Overbeek(XDLVO) theory and physicochemical properties were combined to assess the aggregation ability of sludge during aerobic granulation process qualitatively and quantitatively. Results show that relative hydrophobicity of sludge and polysaccharide content of extracellular polymeric substances(EPS) increased, while electronegativity of sludge decreased during acclimation phase. After 20 days' acclimation, small granules began to form due to high aggregation ability of sludge. Since then, coexisted flocs and granules possessed distinct physicochemical properties during granulation and maturation phase. The relative hydrophobicity decreased while electronegativity increased for flocs, whereas that for granules presented reverse trend. Through analyzing the interaction energy using the XDLVO theory, small granules tended to self-grow rather than self-aggregate or attach of flocs due to poor aggregation ability between flocs and granules during the granulation phase. Besides, remaining flocs were unlikely to self-aggregate owing to poor aggregation ability, low hydrophobicity and high electronegativity. | Shasha Yuan Mingming Gao Hui Ma Muhammad Zaheer Afzal Yun-Kun Wang Mingyu Wang Hai Xu Shu-Guang Wang Xin-Hua Wang | 2018 | Journal of Environmental Sciences2018，30，5： | 4 |
| 12 | Matrix stiffness modulates tip cell formation through the p-PXN-Rac1-YAP signaling axis显示文摘Endothelial tip cell outgrowth of blood-vessel sprouts marks the initiation of angiogenesis which is critical in physiological and pathophysiological procedures.However,how mechanical characteristics of extracellular matrix(ECM)modulates tip cell formation has been largely neglected.In this study,we found enhanced CD31 expression in the stiffening outer layer of hepatocellular carcinoma than in surrounding soft tissues.Stiffened matrix promoted sprouting from endothelial cell(EC)spheroids and upregulated expressions of tip cell-enriched genes in vitro.Moreover,tip cells showed increased cellular stiffness,more actin cytoskeleton organization and enhanced YAP nuclear transfer than stalk and phalanx ECs.We further uncovered that substrate stiffness regulates FAK and Paxillin phosphorylation in focal adhesion of ECs promoting Rac1 transition from inactive to active state.YAP is subsequently activated and translocated into nucleus,leading to increased tip cell specification.p-Paxillin can also loosen the intercellular connection which also facilitates tip cell specification.Collectively our present study shows that matrix stiffness modulates tip cell formation through p-PXN-Rac1-YAP signaling axis,shedding light on the role of mechanotransduction in tip cell formation.This is of special significance in biomaterial design and treatment of some pathological situations. | Yaru Guo Feng Mei Ying Huang Siqin Ma Yan Wei Xuehui Zhang Mingming Xu Ying He Boon Chin Heng Lili Chen Xuliang Deng | 2022 | Bioactive Materials2022，7，1： | 4 |
| 13 | Serum macrophage migration inhibitory factor as a potential biomarker to evaluate therapeutic response in patients with allergic asthma:an exploratory study显示文摘Background:Previous studies have shown that macrophage migration inhibitory factor(MIF)is involved in the pathogenesis of asthma.This study aimed to investigate whether serum MIF reflects a therapeutic response in allergic asthma.Methods:We enrolled 30 asthmatic patients with mild-to-moderate exacerbations and 20 healthy controls,analyzing the parameter levels of serum MIF,serum total immunoglobulin E(tIgE),peripheral blood eosinophil percentage(EOS%),and fractional exhaled nitric oxide(FeNO).Lung function indices were used to identify disease severity and therapeutic response.Results:Our study showed that all measured parameters in patients were at higher levels than those of controls.After one week of treatment,most parameter levels decreased significantly except for serum tIgE.Furthermore,we found that serum MIF positively correlated with EOS%as well as FeNO,but negatively correlated with lung function indices.Receiver operator characteristic(ROC)curve analysis indicated that among the parameters,serum MIF exhibited a higher capacity to evaluate therapeutic response.The area under the curve(AUC)of MIF was 0.931,with a sensitivity of 0.967 and a specificity of 0.800.Conclusions:Our results suggested that serum MIF may serve as a potential biomarker for evaluating therapeutic response in allergic asthma with mild-to-moderate exacerbations. | Huiyuan ZHU Shaochun YAN Jingshuo WU Zhong ZHANG Xiaolin LI Zheng LIU Xing MA Lina ZHOU Lin ZHANG Mingming FENG Yiwei GENG Aixin ZHANG Sabina JANCIAUSKIENE Aiguo XU | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，6： | 3 |
| 14 | Present Situation and Control of Outbreaks of Algal Blooms in the Three Gorges Reservoir显示文摘After water storage in the Three Gorges Reservoir Region,there are no outbreaks of algal blooms in the main stream of the reservoir region,but the density of algae increases obviously. Outbreaks of algal blooms mainly appeared in the tributaries of the reservoir region such as the Xiangxi River,Daning River,Shennong River and Xiaojiang River,but they did not occur every year. The reasons for outbreaks of algal blooms in the tributaries are shown as follows: the existence of sources of algae(blue-green algae) in the Three Gorges Reservoir is the root cause,and the sources include sources existing and being produced in the reservoir and sources from upstream main stream and its tributaries and other related lakes and reservoirs,of which the sources are mainly from the Dianchi Lake; slight or moderate eutrophication of water is the basic condition;hydrologic and hydrodynamic conditions and suitable temperature are conducive to proliferation and aggregation of algae(blue-green algae) after the operation of the Three Gorges Reservoir until outbreaks of algal blooms appear. Outbreaks of algal blooms in the tributaries of the Three Gorges Reservoir Region mainly appear in backwater reaches; they mainly occur in the tributaries in the north of the reservoir region and near to the dam;they mainly appear from March to July; the dominant species of algae( blue-green algae) in the Three Gorges Reservoir are Pyrrophyta,Bacillariophyta and Chlorophyta,but they tend to change into blue-green algae and other algae. To control outbreaks of algal blooms in the tributaries of the Three Gorges Reservoir Region,it is needed to prevent water containing blue-green algae collected from the Dianchi Lake and other lakes and reservoirs from being input into the lower reaches,reduce pollution load flowing into the Three Gorges Reservoir,use enclosures to change hydrodynamic conditions of backwater reaches of the tributaries appropriately,and adopt biological measures such as culturing fish and planting plants to improve ecosystem of the tributaries and other measures to inhibit and eliminate algae and decrease eutrophication level. | Ma Jianhua Hu Mingming Zhu Xi | 2016 | Meteorological and Environmental Research2016，7，6： | 3 |
| 15 | Synthetic FXR Agonist GW4064 Prevents Diet-Induced Hepatic Steatosis and Insulin Resistance显示文摘 | Yongjie Ma Yixian Huang Linna Yan Mingming Gao Dexi Liu | 2013 | Pharmaceutical Research2013，，5： | 3 |
| 16 | Innovation Requirements and Progress of Dialysis Water Treatment System显示文摘With the development of medicine,people are becoming more and more aware of the quality of medical water supply,such as dial ysis water,dental water,preparation water,operating room water,supply room water.Therefore,People are constantly improving the water treatment system and its standardized,and the demand for dialysis water is also becoming more and more strict.Hemodialysis is an effective means of maintaining the life of renal failure patients,120~180L water will be used for a conventional dialysis treatment,and high flux dialysis will consume more. | Jun Ke Lifong Yan Taksui Wong Bo Hu Shuang Cui Sibo Huang Aiyun Cha Jie Huang Hongwei Hu Dawen Yun Shaofeng Huang Jie Shu Mingming Ma Lianghong Yin Xiangnan Dong Chen Yun Zuhui Chen Fanna Liu | 2017 | 临床医学工程2017，24，S1： | 2 |
| 17 | Application and optimization of solid-state fermentation process for enhancing polygalacturonase production by Penicillium expansum显示文摘Penicillium expansum 3.5425 was applied in solid-state fermentation(SSF)of agricultural wastes for polygalacturonase biosynthesis.Among various carbon additives,apple pomace was most suitable for the biosynthesis of polygalacturonase(1440.57 U/g).Optimization of medium parameters using rotational orthogonal design(ROD)experiment combined with optimal fermentation conditions resulted in a 2.72-fold increase in the polygalacturonase production.By using ammonium sulphate precipitation,ion-exchange and gel-permeation chromatography,the polygalacturonase produced by P.expansum 3.5425 was finally purified which had specific activity of 19269 U/mg and molecular weight of 30 kDa.The enzyme was remarkably active in the pH range of 3-5 and at 50℃,which makes it more acceptable in the industrial application.Besides,partially purified polygalacturonase(875.15 U/mL)was used for apple juice clarification and the clarity at 0.4 mL/kg was maximum,which reveals a great potential of polygalacturonase in food industry. | Mingming Zhu Hongju He Mingtao Fan Hanjun Ma Haiwei Ren Jie Zeng Haiyan Gao | 2018 | International Journal of Agricultural and Biological Engineering2018，11，6： | 2 |
| 18 | Identification of aeolian loess deposits on the Indo-Gangetic Plain(India)and their significance显示文摘Aeolian loess deposits contain abundant information about the evolution of the paleoenvironment.For example,paleoclimate changes recorded in Chinese loess area obtained significant achievement in the past few decades.Compared to Chinese loess,research on Indian loess is lacking.Currently,most studies focus on the Kashmir area located in the southern Himalayas,and studies on other areas are rare.However,field observations demonstrate that the sediments around the New Delhi-Agra-Jaipur Plain are similar to Chinese loess-paleosol sequences.For example,the boundary between two strata is transitional and without horizontal bedding.Moreover,obvious pedogenic horizons developed among sediment sequences,probably indicating unrecognised aeolian deposits in the Indo-Gangetic Plain(IGP).To confirm this,pilot samples were obtained from the IGP and detailed indoor measurements conducted.The results indicate that the distribution patterns of particle size and rare earth elements(REE) of the pilot samples are similar to Chinese loess.Furthermore,the scanning electronic microscopy(SEM) images of pilot samples show obvious conchoidal fractures,dash-shaped concavities,and abundant small pits that usually form through mechanical impact.These are typical characteristics of aeolian particles.In addition,environmental and rock magnetic measurements indicate that the dominant magnetic minerals in the pilot samples are magnetite and maghemite,and that they likely contain small amounts of hematite.Furthermore,conventional magnetic parameters are comparable with Chinese loess.Based on this,aeolian loess deposits are widely distributed in the IGP,which may have promoted the development of Indian farming and contributed towards the prosperity of ancient Indian civilisation.This study also provides a new and valuable record for the research on paleoclimate changes in the study area in the future. | LIU XiuMing MA MingMing WU HaiBin ZHOU ZiBo | 2017 | Science China Earth Sciences2017，60，3： | 2 |
| 19 | Citron C-05 inhibits both the penetration and colonization of Xanthomonas citri subsp.citri to achieve resistance to citrus canker disease显示文摘Citrus canker,caused by Xanthomonas citri subsp.citri(Xcc),is a serious bacterial disease that affects citrus production worldwide.Citron C-05(Citrus medica)is the only germplasm in the Citrus genus that has been identified to exhibit strong resistance to Xcc.However,it has not been determined when,where,and how Xcc is restricted in the tissues of Citron C-05 during the infection process.In the present study,we investigated the spatiotemporal growth dynamics of an eGFP-labeled virulent Xcc(eGFP-Xcc)strain in Citron C-05 along with five susceptible biotypes(i.e.,lemon,pummelo,sour orange,sweet orange,and ponkan mandarin)upon inoculation via the spraying or leaf infiltration of a bacterial suspension.The results from extensive confocal laser scanning microscopy analyses showed that while Xcc grew rapidly in plants of all five susceptible genotypes,Xcc was severely restricted in the epidermal and mesophyll cell layers of the leaves of Citron C-05 in the early stage of infection.Not surprisingly,resistance against Xcc in Citron C-05 was found to be associated with the production of reactive oxygen species and hypersensitive response-like cell death,as well as greater upregulation of several defense-related genes,including a pathogenesis-related gene(PR1)and a glutathione S-transferase gene(GST1),compared with sweet orange as a susceptible control.Taken together,our results not only provide further valuable details of the spatiotemporal dynamics of the host entry,propagation,and spread of Xcc in both resistant and susceptible citrus plants but also suggest that resistance to Xcc in Citron C-05 may be attributed to the activation of multiple defense mechanisms. | Hongyan Fu Mingming Zhao Jing Xu Limei Tan Jian Han Dazhi Li Meijun Wang Shunyuan Xiao Xianfeng Ma Ziniu Deng | 2020 | Horticulture Research2020，7，1： | 2 |
| 20 | LW-NIR hyperspectral imaging for rapid prediction of TVC in chicken flesh显示文摘Total viable count(TVC)is often used as an important indicator for chicken freshness evaluation.In this study,112 fresh chicken flesh samples were acquired after slaughtered and their hyperspectral images were collected in the LW-NIR(900-1700 nm)range.The full LW-NIR spectra(486 wavebands)within the images were extracted and applied to related to reference TVC values measured in different storage periods,using partial least squares regression(PLSR)algorithm,resulting in high correlation coefficients(R)and low root mean square errors(RMSE),for either raw spectra or pretreatment spectra.By using regression coefficients(RC)method,20,18,17 and 20 optimal wavebands were respectively selected from raw spectra,baseline correction(BC)spectra,Savitzky-Golay convolution smoothing(SGCS)spectra and standard normal variate(SNV)spectra and applied for the optimization of original full waveband PLSR model.By comparison,RC-PLSR model based on the SGCS spectra showed a better performance in TVC prediction with RC of 0.98 and RMSEC of 0.35 log10 CFU/g in calibration set,and RP of 0.98 and RMSEP of 0.44 log10 CFU/g in prediction set.At last,by transferring the best RC-PLSR model,the dynamic TVC change during the storage was visualized by color maps to indicate the TVC spoilage degree.The overall study revealed that LW-NIR hyperspectral imaging combined with PLSR could be used to predict the freshness of chicken flesh. | Hui Wang Hongju He Hanjun Ma Fusheng Chen Zhuangli Kang Mingming Zhu Zhengrong Wang Shengming Zhao Rongguang Zhu | 2019 | International Journal of Agricultural and Biological Engineering2019，12，3： | 2 |
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