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| 1 | Rumen microbiome structure and metabolites activity in dairy cows with clinical and subclinical mastitis显示文摘Background:Due to the high prevalence and complex etiology,bovine mastitis(BM)is one of the most important diseases to compromise dairy cow health and milk quality.The shift in milk compositions has been widely investigated during mastitis,but recent studies suggested that gastrointestinal microorganism also has a crucial effect on the inflammation of other peripheral tissues and organs,including the mammary gland.However,research focused on the variation of rumen inner-environment during mastitis is still limited.Therefore,the ruminal microbial profiles,metabolites,and milk compositions in cows with different udder health conditions were compared in the present study.Furthermore,the correlations between udder health status and ruminal conditions were investigated.Based on the somatic cell counts(SCC),California mastitis test(CMT)parameters and clinical symptoms of mastitis,60 lactating Holstein dairy cows with similar body conditions(excepted for the udder health condition)were randomly divided into 3 groups(n=20 per group)including the healthy(H)group,the subclinical mastitis(SM)group and the clinical mastitis(CM)group.Lactation performance and rumen fermentation parameters were recorded.And rumen microbiota and metabolites were also analyzed via 16S rRNA amplicon sequencing and untargeted metabolomics,respectively.Results:As the degree of mastitis increased,rumen lactic acid(LA)(P<0.01),acetate,propionate,butyrate,valerate(P<0.001),and total volatile fatty acids(TVFAs)(P<0.01)concentrations were significantly decreased.In the rumen of CM cows,the significantly increased bacteria related to intestinal and oral inflammation,such as Lachnospiraceae(FDR-adjusted P=0.039),Moraxella(FDR-adjusted P=0.011)and Neisseriaceae(FDR-adjusted P=0.036),etc.,were accompanied by a significant increase in 12-oxo-20-dihydroxy-leukotriene B4(FDR-adjusted P=5.97×10^(−9))and 10beta-hydroxy-6beta-isobutyrylfuranoeremophilane(FDR-adjusted P=3.88×10^(−10)).Meanwhile,in the rumen of SM cows,the Ruminiclostridium_9(FDR-adjusted P=0.042)and Enterorhabdus(FDR-adjusted P=0.043)were increased along with increasing methenamine(FDR-adjusted P=6.95×10^(−6)),5-hydroxymethyl-2-furancarboxaldehyde(5-HMF)(FDR-adjusted P=2.02×10^(−6))and 6-methoxymellein(FDR-adjusted P=2.57×10^(−5)).The short-chain fatty acids(SCFAs)-producing bacteria and probiotics in rumen,including Prevoterotoella_1(FDRadjusted P=0.045)and Bifidobacterium(FDR-adjusted P=0.035),etc.,were significantly reduced,with decreasing 2-phenylbutyric acid(2-PBA)(FDR-adjusted P=4.37×10^(−6)).Conclusion:The results indicated that there was a significant shift in the ruminal microflora and metabolites associated with inflammation and immune responses during CM.Moreover,in the rumen of cows affected by SM,the relative abundance of several opportunistic pathogens and the level of metabolites which could produce antibacterial compounds or had a competitive inhibitory effect were all increased. | Yue Wang Xuemei Nan Yiguang Zhao Linshu Jiang Mengling Wang Hui Wang Fan Zhang Fuguang Xue Dengke Hua Jun Liu Junhu Yao Benhai Xiong | 2021 | Journal of Animal Science and Biotechnology2021，12，3： | 6 |
| 2 | Microstructure and properties of Ti-6Al-4V fabricated by low-power pulsed laser directed energy deposition显示文摘Thin-wall structures of Ti-6A1-4V were fabricated by low-power pulsed laser directed energy deposition. During deposition, consistent with prior reports, columnar grains were observed which grew from the bottom toward the top of melt pool tail. This resulted in a microstructure mainly composed of long and thin prior epitaxial β columnar grains (average width ^200μm). A periodic pattern in epitaxial growth of grains was observed, which was shown to depend upon laser traverse direction. Utilizing this, a novel means was proposed to determine accurately the fusion boundary of each deposited layer by inspection of the periodic wave patterns. As a result it was applied to investigate the influence of thermal cycling on microstructure evolution. Results showed that acicular martensite,α' phase, and a small amount of Widmanstatten, a laths, gradually converted to elongated acicular a and a large fraction of Widmanstatten a laths under layer-wise thermal cycling. Tensile tests showed that the yield strength, ultimate tensile strength and elongation of Ti-6Al-4V thin wall in the build direction were 9.1 %, 17.3% and 42% higher respectively than those typically observed in forged solids of the same alloy. It also showed the yield strength and ultimate tensile strength of the transverse tensile samples both were 13.3% higher than those from the build direction due to the strengthening effect of a large number of vertical β grain boundaries, but the elongation was 69.7% lower than that of the build direction due to the uneven grain deformation of β grains. | Hua Tan Mengle Guo Adam T. Clare Xin Lin Jing Chen Weidong Huang | 2019 | Journal of Materials Science & Technology2019，35，9： | 5 |
| 3 | The cytotoxicity and protective effects of Astragalus membranaceus extracts and butylated hydroxyanisole on hydroxyl radical-induced apoptosis in fish erythrocytes显示文摘Erythrocytes play an essential role in transporting O_2 and CO_2 for respiration in fish. However,erythrocytes continuously suffer from reactive oxygen species(ROS)-induced oxidative stress and apoptosis. Thus, it is essential to expand our knowledge of how to protect erythrocytes against ROS-induced oxidative stress and apoptosis in fish. In this study, we explored the cytotoxicity and the effects of butylated hydroxyanisole(BHA), ethyl ether extracts, ethyl acetate extracts, acetone extracts(AE), ethanol extracts, and aqueous extracts of Astragalus membranaceus(EAm) on hydroxyl radical(·OH)-induced apoptosis in carp erythrocytes. The rat hepatocytes and carp erythrocytes were incubated with different concentrations of BHA or EAm(0.125 to 1 mg/mL). The toxicity in rat hepatocytes and carp erythrocytes was then measured using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay and a haemolysis assay,respectively. The carp erythrocytes were treated with BHA or EAm in the presence of 40 μmol/L FeSO4 and20 μmol/L H_2 O_2 at 37℃, except for the control group. Oxidative stress and apoptosis parameters in the carp erythrocytes were then evaluated using the commercial kit. The results indicated that at high concentrations, BHA and EAm could induce toxicity in rat hepatocytes and fish erythrocytes. However, BHA was more toxic than EAm at the same concentrations. Moreover, the toxicity order of BHA and EAm in the fish erythrocytes approximately agreed with that for the rat hepatocytes. Butylated hydroxyanisole and EAm suppressed the ·OH-induced phosphatidylserine exposure and DNA fragmentation(the biomarkers of apoptosis) by decreasing the generation of ROS, inhibiting the oxidation of cellular components, and restoring the activities of antioxidants in carp erythrocytes. Of all of the examined EAm, the AE showed the strongest effects. The effects of AE on superoxide anion, H_2 O_2, met-haemoglobin and reduced glutathione levels, as well as glutathione reductase activity and apoptosis were equivalent to or stronger than those of BHA. These results revealed that the AE of Astragalus membranaceus could be used as a potential natural antioxidant or apoptosis inhibitor in fish erythrocytes. | Huatao Li Xiaoqiu Zhou Min Wu Mengling Deng Chao Wang Jingjing Hou Pengju Mou | 2016 | Animal Nutrition2016，，4： | 5 |
| 4 | A continuous and high-efficiency process to separate coal bed methane with porous ZIF-8 slurry:Experimental study and mathematical modelling显示文摘Coal bed methane has been considered as an important energy resource.One major difficulty of purifying coal bed methane comes from the similar physical properties of CH_4 and N_2.The ZIF-8/water-glycol slurry was used as a medium to separate coal bed methane by fluidifying the solid adsorbent material.The sorption equilibrium experiment of binary mixture(CH_4/N_2)and slurry was conducted.The selectivity of CH_4 to N_2 is within the range of 2-6,which proved the feasibility of the slurry separation method.The modified Langmuir equation was used to describe the gas-slurry phase equilibrium behavior,and the calculated results were in good agreement with the experimental data.A continuous absorption-adsorption and desorption process on the separation of CH_4/N_2 in slurry is proposed and its mathematical model is also developed.Sensitivity analysis is conducted to determine the operation conditions and the energy performance of the proposed process was also evaluated.Feed gas contains 30 mol%of methane and the methane concentration in product gas is 95.46 mol%with the methane recovery ratio of 90.74%.The total energy consumption for per unit volume of product gas is determined as 1.846 kWh Nm^(-3).Experimental results and process simulation provide basic data for the design and operation of pilot and industrial plant. | Wan Chen Xiaonan Guo Enbao Zou Mengling Luo Mengzijing Chen Mingke Yang Hai Li Chongzhi Jia Chun Deng Changyu Sun Bei Liu Lanying Yang Guangjin Chen | 2020 | Green Energy & Environment2020，5，3： | 5 |
| 5 | Anisotropic NdFeB/SmCoCuFeZr composite bonded magnet prepared by warm compaction process显示文摘Anisotropic NdFeB/SmCoCuFeZr composite bonded magnets were prepared by warm compaction process. The effects of adding SmCoCuFeZr magnetic powder on the properties of anisotropic bonded NdFeB magnet were investigated in this work. The results show that, both magnetic properties and temperature stability of the bonded magnet can be improved by adding fine SmCoCuFeZr magnetic powder. In the present study, the optimal content of SmCoCuFeZr magnetic powder was about 20 wt.%, in this case, the Br, Hcj, and(BH)maxof the NdFeB/SmCoCuFeZr composite magnet achieved 0.943 T, 1250 kA/m, and168 kJ/m^3, respectively. | Mengling Wu Yuping Li Xin Wang Lizhao Chen Yaozhao Mu | 2017 | Journal of Rare Earths2017，35，12： | 4 |
| 6 | Light-emitting diodes based on all-inorganic copper halide perovskite with self-trapped excitons显示文摘Light-emitting diodes based on lead halide perovskite have attracted great attention due to their outstanding performance.However,their application is plagued by the toxicity of Pb and the poor stability.Herein novel copper-based all inorganic perovskite CsCu2I3 with much enhanced stability has been reported with a potential photoluminescence quantum yield(PLQY)over 20%and self-trapped excitons(STE).By taking advantage of its extraordinary thermal stability,we successfully fabricate high-quality CsCu2I3 film through direct vacuum-based deposition(VBD)of CsCu2I3 powder.The resulting film shows almost the same PLQY with the synthesized powder,as well as excellent uniformity and stability.The perovskite light-emitting diodes(Pe-LED)based on the evaporated CsCu2I3 emitting layer achieve a luminescence of 10 cd/m2 and an external quantum efficiency(EQE)of 0.02%.To the best of our knowledge,this is the first CsCu2I3 Pe-LED fabricated by VBD with STE property,which offers a new avenue for lead-free Pe-LED. | Nian Liu Xue Zhao Mengling Xia Guangda Niu Qingxun Guo Liang Gao Jiang Tang | 2020 | Journal of Semiconductors2020，41，5： | 4 |
| 7 | E. coli and bacteriophage MS2 disinfection by UV, ozone and the combined UV and ozone processes显示文摘低剂量的臭氧与的联合紫外(紫外) 照耀应该是一种选择把利益给消毒和还原剂缺点紫外并且臭氧消毒。然而,少些对消毒表演被知道紫外并且臭氧(UV/ozone ) coexposure 和顺序的 UV-followed-by-ozone (紫外臭氧) 并且 ozone-followed-by-UV (臭氧 -- 紫外) 暴露。在这研究, E 的 inactivation。coli 和抗菌素 MS2 由紫外,臭氧, UV/ozone coexposure,并且顺序紫外臭氧并且臭氧 -- 紫外暴露被调查并且比较。E 上的 0.50.9 木头杀死的 Synergistic 效果。包括率和效率的增加, coli inactivation 在象 0.05 mg 潩 ? 慷 ? 捡畴污祬椠据敲獡摥愠瑦牥挠汨牯湩瑡潩一样低的臭氧集中在 UV/ozone coexposure 以后被观察吗? | Jingyun FANG Huiling LIU Chii SHANG Minzhen ZENG Mengling NI Wei LIU | 2014 | Frontiers of Environmental Science & Engineering2014，8，4： | 3 |
| 8 | Advances in Studies on Interaction between Selenium and Heavy Metal Cadmium显示文摘Selenium is an essential functional element of the human body,and the issue of selenium accompanying heavy metals is receiving much concern.This paper expounded the form of existence of selenium and cadmium in soil and influencing factors of their bioavailability,and further elaborated the interaction between selenium and cadmium.On the basis,it provided references for further studies on the interaction between selenium and cadmium,and provided basis for establishing selenium-enriched barrier technology for crops. | Liping PAN Yongxian LIU Yanfei HUANG Mengling NONG Shiyang LU Jinping CHEN Yuying ZHAO Liumei XIONG Xiu LAN Kebing LI | 2017 | Asian Agricultural Research2017，9，8： | 3 |
| 9 | Daifan San intervenes in forkhead box P3 and the interleukin(IL)-23/IL-17A signaling pathway to help prevent and treat primary biliary cirrhosis显示文摘OBJECTIVE:To investigate the mechanism by which Daifan San(DFS)prevents and treats primary biliary cirrhosis(PBC)via the forkhead box P3(FoxP3)and interleukin(IL)-23/IL-17A signaling pathways.METHODS:Ninety C57BL/6 mice were randomly divided into the control,model,DFS low-dose,DFS middle-dose,DFS high-dose and ursodeoxycholic acid(UDCA)groups(n=15 per group).A mouse model of PBC was induced using polyinosinic polycytidylic acids(poly I:C).Lymphocyte subset expression in the peripheral blood was analyzed via flow cytometry.The inflammatory cytokines and antimitochondrial autoantibody(AMA)levels were detected via enzyme-linked immunosorbent assays.The expressions and location of typeⅠcollagen,typeⅢcollagen,cytokeratin 19 and FoxP3 in the liver tissue were evaluated via immunohistochemistry.FoxP3,IL-23 and IL-17 expressions in the peripheral blood and liver tissue were evaluated via real-time polymerase chain reaction and western blotting.RESULTS:IL-17,IL-23,IL-8,IL-33,TNF-α,and AMA expressions were significantly increased in the model group and decreased in the DFS and UDCA groups.Conversely,Treg cell and FoxP3 expressions were significantly decreased in the model group and increased in the DFS and UDCA groups.The IL-23/IL-17A signaling pathway was closely correlated with chronic inflammation of the bile duct in PBC and functional deletion of Treg cells,leading to reduced FoxP3 levels and mediating the loss of tolerance in PBC.CONCLUSION:DFS may delay the occurrence and relieve the symptoms of PBC by downregulating IL-23/IL-17A signaling pathway expression and upregulating FoxP3 expression. | Zhan Kai Xu Yan Han Mengling Cheng Liangbin | 2020 | Journal of Traditional Chinese Medicine2020，40，4： | 3 |
| 10 | Rail temperature rise characteristics caused by linear eddy current brake of high-speed train显示文摘The rail temperature rises when the linear eddy current brake of high-speed train is working,which may lead to a change of rail physical characteristics or an effect on train operations.Therefore,a study concerning the characteristics of rail temperature rise caused by eddy current has its practical necessity.In the research,the working principle of a linear eddy current brake is introduced and its FEA model is established.According to the generation mechanism of eddy current,the theoretical formula of the internal energy which is produced by the eddy current is deduced and the thermal load on the rail is obtained.ANSYS is used to simulate the rail temperature changes under different conditions of thermal loads.The research result shows the main factors which contribute to the rising of rail temperature are the train speed,brake gap and exciting current.The rail temperature rises non-linearly with the increase of train speed.The rail temperature rise curve is more sensitive to the exciting current than the air gap.Moreover,the difference stimulated by temperature rising between rails of 60 kg/m and 75 kg/m is presented as well. | Xiaoshan Lu Yunfeng Li Mengling Wu Jianyong Zuo Wei Hu | 2014 | Journal of Traffic and Transportation Engineering(English Edition)2014，1，6： | 3 |
| 11 | Organic–inorganic hybrid perovskite scintillators for mixed field radiation detection显示文摘Sensitive and fast detection of neutrons and gamma rays is vital for homeland security,high-energy physics,and proton therapy.Fast-neutron detectors rely on light organic scintillators,andγ-ray detectors use heavy inorganic scintillators and semiconductors.Efficient mixed-field detection using a single material is highly challenging due to their contradictory requirements.Here we report hybrid perovskites(C_(8)H_(12)N)_(2)Pb(Br_(0.95)Cl_(0.05))_(4)that combine light organic cations and heavy inorganic skeletons at a molecular level to achieve unprecedented performance for mixed-field radiation detection.High neutron absorption due to a high density of hydrogen,strong radiative recombination within the highly confined[PbX_(6)]^(4-)layer,and sub-nanometer distance between absorption sites and radiative centers,enable a light yield of 41000 photons/MeV,detection pulse width of 2.97 ns and extraordinary linearity response toward both fast neutrons andγ-rays,outperforming commonly used fast-neutron scintillators.Neutron energy spectrum,time-of-flight based fast-neutron/γ-ray discrimination and neutron yield monitoring were all successfully achieved using(C_(8)H_(12)N)_(2)Pb(Br_(0.95)Cl_(0.05))_(4)detectors.We further demonstrate the monitoring of reaction kinetics and total power of a nuclear fusion reaction.We envision that molecular hybridized scintillators open a new avenue for mixed-field radiation detection and imaging. | Mengling Xia Guangda Niu Linyue Liu Runlong Gao Tong Jin Pengying Wan Weicheng Pan Xianpeng Zhang Zuoxiang Xie Sam Teale Zenghua Cai Jiajun Luo Shan Zhao Haodi Wu Shiyou Chen Zhiping Zheng Qingguo Xie Xiaoping Ouyang Edward HSargent Jiang Tang | 2022 | InfoMat2022，4，9： | 2 |
| 12 | The Pumilio RNA-binding protein APUM24 regulates seed maturation by fine-tuning the BPM-WRI1 module in Arabidopsis显示文摘Pumilio RNAbindingproteinsparticipateinmes-senger RNA(mRNA)degradation and t ranslational repression,but their roles in plant development are largely unclear.Here,we show that Arabidopsis PUMILIO PROTEIN24(APUM24),an atypical Pumiliohomology domaincontaining protein,plays an im-portant part in regulating seed maturation,a major stage of plant development.APUM24 is strongly expressed in maturing seeds.Reducing APUM24 expression resulted in abnormal seed maturation,wrinkled seeds,and lower seed oil contents,and APUM24 knockdown resulted in lower levels of WRINKLED 1(WRI1),a key transcription factor con-trolling seed oil accumulation,and lower expression of WRI1 target genes.APUM24 reduces the mRNA stability of BTB/POZMATH(BPM)family genes,thus decreasing BPM protein levels.BPM is responsible for the 26S proteasomemediated degradation of WRI1 and has important functions in plant growth and development.The 3′untranslated regions of BPM family genes contain putative Pumilio response elements(PREs),which are bound by APUM24.Re-duced BPM or increased WRI1 expression rescued the decient seed maturation of apum242 knock-down mutants,and APUM24 overexpression re-sulted in increased seed size and weight.Therefore,APUM24 is crucial to seed maturation through its action as a positive regulatornetuning the BPMWRI1 module,making APUM24 apromising target for breeding strategies to increase crop yields. | Ruihua Huang Mengling Liu Guanping Gong Pingzhi Wu Barunava Patra Ling Yuan Hongting Qin Xiaoxu Wang Guohe Wang Huimei Liao Lu Gao Chengwei Yang Hongqing Li Shengchun Zhang | 2021 | Journal of Integrative Plant Biology2021，63，7： | 2 |
| 13 | Partial root-zone irrigation enhanced soil enzyme activities and water use of maize under different ratios of inorganic to organic nitrogen fertilizers显示文摘 | Fusheng Li Jiangmin Yu Mengling Nong Shaozhong Kang Jianhua Zhang | 2009 | Agricultural Water Management2009，，2： | 2 |
| 14 | Exponential stability of the Euler-Maruyama method for neutral stochastic functional differential equations with jumps显示文摘The exponential stability of trivial solution and numerical solution for neutral stochastic functional differential equations(NSFDEs) with jumps is considered. The stability includes the almost sure exponential stability and the mean-square exponential stability. New conditions for jumps are proposed by means of the Borel measurable function to ensure stability. It is shown that if the drift coefficient satisfies the linear growth condition, the Euler-Maruyama method can reproduce the corresponding exponential stability of the trivial solution. A numerical example is constructed to illustrate our theory. | Haoyi MO Mengling LI Feiqi DENG Xuerong MAO | 2018 | Science China(Information Sciences)2018，61，7： | 2 |
| 15 | Basic theory and stability analysis for neutral stochastic functional differential equations with pure jumps显示文摘This paper investigates the existence and uniqueness of solutions to neutral stochastic functional differential equations with pure jumps(NSFDEwPJs). The boundedness and almost sure exponential stability are also considered. In general, the classical existence and uniqueness theorem of solutions can be obtained under a local Lipschitz condition and linear growth condition. However, there are many equations that do not obey the linear growth condition. Therefore, our first aim is to establish new theorems where the linear growth condition is no longer required whereas the upper bound for the diffusion operator will play a leading role. Moreover, the pth moment boundedness and almost sure exponential stability are also obtained under some loose conditions. Finally, we present two examples to illustrate the effectiveness of our results. | Mengling LI Feiqi DENG Xuerong MAO | 2019 | Science China(Information Sciences)2019，62，1： | 2 |
| 16 | Regulation of tumor necrosis factor-related apoptosis-inducing ligand sensitivity in primary and transformed human keratinocytes显示文摘 | Leverkus M Neumann M Mengling T | 2000 | Cancer Res2000，60，： | 1 |
| 17 | Genetic variation and phylogenetic relationships of 22 porcine reproductive and respiratory syndrome virus (PRRSV) field strains based on sequence analysis of open reading frame 5 显示文摘 | Andreyev V G Wesley R D Mengling W L | 1997 | Arch Virol1997，142，5： | 1 |
| 18 | Successful combination of local CpG-ODN and radiotherapy in maligmant glioma显示文摘 | Yuxia Mengl Antoine F Carpentier | 2005 | Int J Cancer2005，116，： | 1 |
| 19 | Efficacy of an in-activated vaccine for Prevention of repro- ductive failure induced by porcine reproductive and respiratory syndrome virus显示文摘 | Plana Duran Collins J E Mengling W L | 1997 | Vet Microbiol1997，55，： | 1 |
| 20 | Biological assay of attenuated strain NADL- 2 and virulent strain NADL- 8 of porcine parvovirus显示文摘 | Mengling W L Pejsak Z Paul P S | 1984 | Am J vet Res1984，45，11： | 1 |
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