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| 1 | Reversal of the phenotype by K-ras^(val12) silencing mediated by adenovirus-delivered siRNA in human pancreatic cancer cell line Panc-1显示文摘AIM: To investigate the in vitro antitumor effect of adenovirus-mediated small interfering RNAs (siRNAs) on pancreatic cancer and the associated mechanism.METHODS: A 63-nucleotide (nt) oligonucleotide encoding K-ras^val12 and specific siRNA were introduced into pSilencer 3.1-H1, then the H1-RNA promoter and siRNA coding insert were subcloned into pAdTrack to get plasmid pAdTrackH1-K-ras^val12. After homologous recombination in bacteria and transfections of such plasmids into a mammalian packaging cell line 293, siRNA expressing adenovirus AdH1-K-ras^val12 was obtained. Stable suppression of K-ras^val2 was detected by Northern blot and Western blot. Apoptosis in Panc-1 cells was detected by flow cytometry.RESULTS: We obtained adenovirus AdH1-K-ras^val12 carrying the pSilencer 3.1-H1 cassette, which could mediate gene silencing. Through siRNA targeted K-ras^val12, the oncogenic phenotype of cancer cells was reversed. Flow cytometry showed that apoptotic index of Panc-1 cells was significantly higher in the AdH1-K-ras^val12-treatment group (18.70% at 72 h post-infection, 49.55% at 96 h post-infection)compared to the control groups (3.47%, 3.98% at 72 and 96 h post-infection of AdHl-empty, respectively, 4.21%,3.78% at 72 and 96 h post-infection of AdH1-p53,respectively) (P<0.05).CONCLUSION: These results demonstrate that adenoviral vectors can be used to mediate RNA interference (RNAi)to induce persistent loss of functional phenotypes. In gene therapy, the selective down-regulation of only the mutant version of a gene allows for highly specific effects on tumor cells, while leaving the normal cells untouched. In addition, the apoptosis of pancreatic cancer cell line Panc-1 can be induced after AdH1-K-ras^val12 infection. This kind of adenovirus based on RNAi might be a promising vector for cancer therapy. | Li-MoChen Huang-YingLe Ren-YiQin ManojKumar Zhi-YongDu Rui-JuanXia JingDeng | 2005 | World Journal of Gastroenterology2005，11，6： | 22 |
| 2 | Antiangiogenic effect of somatostatin receptor subtype 2 on pancreatic cancer cell line:Inhibition of vascular endothelial growth factor and matrix metalloproteinase-2 expression in vitro显示文摘AIM:To investigate the anti-angiogenic effect of somatostatin receptor subtype 2 (SSTR2) gene transfer into pancreatic cancer cell line PC-3, and the mechanisms involved in this effect.METHODS: The full length human SSTR2 cDNA was introduced into pancreatic cancer cell line PC-3 by lipofectamine-mediated transfection. Positive clones were screened by G418 and stable expression of SSTR2 was detected by immunohistochemistry SABC methods and RT-PCR. Enzyme-linked immunosorbent assay (ELISA) was used to detect vascular endothelial growth factor (VEGF) levels in the cell culture supernatants of SSTR2-expressing cells, vector control and mock control cells. Furthermore, the expressions of VEGF and matrix metalloproteinase-2 (MMP-2) were detected by immunohistochemistry SABC methods and RT-PCR in these cells.RESULTS: VEGF levels in the cell culture supernatants were significantly reduced in the SSTR2-expressing cells (first week,172.63±21.2ng/L and after two months, 198.85±26.44ng/L)compared with the vector control (first week, 790.39±86.52ng/L and after two months, 795.69±72.35ng/L) and mock control (first week, 786.42±90.62ng/L and after two months,805.32±84.36ng/L) (P<0.05).The immunohistochemical assay showed a significant reduction of the integral optical density of VEGF and MMP-2 in the SSTR2-expressing cells (42.25±8.6 and 70.5±6.25, respectively) compared with the vector control (85.75±12.9 and 110.52±13.5, respectively) and mock control (82.6±9.28 and 113.56±9.62,respectively) (P<0.05).Conversely, the average gray value of VEGF and MMP-2 was significantly increased in the SSTR2-expressing cells (121.56±8.43 and 134.46±19.95, respectively) compared with the vector control (55.72±5.6 and 62.26±12.68,respectively) and mock control cells (58.48±6.2 and 65.49±9.16, respectively) (P<0.05). Moreover, the expressions of VEGF mRNA and MMP-2 mRNA were significantly reduced in the SSTR2-expressing cells (0.1384±0.017 and 0.2343±0.070, respectively) compared with the vector control (1.024±0.117 and 0.806±0.119,respectively) and mock control (1.085±0.105 and 0.714±0.079,respectively) (P<0.05).CONCLUSION: The expression of reintroduced human SSTR2 gene exerts its antiangiogenic effects by downregulating the expressions of the factors involved in tumor angiogenesis and metastasis, suggesting SSTR2 gene transfer as a new strategy of gene therapy for pancreatic cancer. | ManojKumar Zheng-RenLiu LaxmiThapa QingChang Da-YuWang Ren-YiQin | 2004 | World Journal of Gastroenterology2004，10，3： | 23 |
| 3 | Strength deformation behaviour of circular concrete filled steel tubes subjected to pure bending显示文摘 | Manojkumar V. Chitawadagi Mattur C. Narasimhan | 2009 | Journal of Constructional Steel Research2009，，8： | 1 |
| 4 | Axial strength of circular concrete-filled steel tube columns — DOE approach显示文摘 | Manojkumar V. Chitawadagi Mattur C. Narasimhan S.M. Kulkarni | 2010 | Journal of Constructional Steel Research2010，，10： | 1 |
| 5 | Molecular approaches for identification and characterization of lactic acid bacteria显示文摘 | DheerajMOHANIA RavinderNAGPAL ManojKUMAR AartiBHARDWAJ MukeshYADAV ShaliniJAIN FrancescoMAROTTA VinodSINGH OmPARKASH HariomYADAV | 2008 | Journal of Digestive Diseases2008，，4： | 1 |
| 6 | Microtubule Plus-End-Tracking Proteins Target Gap Junctions Directly from the Cell Interior to Adherens Junctions显示文摘 | Robin M. Shaw Alex J. Fay Manojkumar A. Puthenveedu Mark von Zastrow Yuh-Nung Jan Lily Y. Jan | 2007 | Cell2007，，： | 1 |
| 7 | Decrease in the concentration of vitamin E in blood and tissues caused by di (2-ethylhexyl) phthalate, a commonly used plasticizer in blood storage bags and medical tubing显示文摘 | Manojkumar V Nair KGP Santhosh A | 1998 | Vox Sanguinis1998，75，2： | 1 |
| 8 | Optimisation of reverse transcriptase loop-mediated isothermal amplification assay for rapid detection of Macrobrachium rosenbergii noda virus and extra small virus in Macrobrachium rosenbergii显示文摘 | DEVIKA P MANOJKUMAR B MOHANAKUMARAN N | 2010 | Journal of Virological Methods2010，167，： | 1 |
| 9 | Optimisation of reverse transcriptase loop - mediated isothermal amplifi- cation assay for rapid detection of Macrobrachium rosenbergii noda virus and extra small virus in Macrobrachium rosenbergii 显示文摘 | Devika P Manojkumar B Mohanakumaran N | 2010 | Journal of Virological Methods2010，167，1： | 1 |
| 10 | Monopolar versus bipolar trans - urethral resection of bladder tumors (TURBT) - A single - centre, parallel ann, randomized controlled trial显示文摘 | Vivek Venkatramani Arabind Panda Ramani Manojkumar | 2013 | The Journal of Urology2013，12，： | 1 |
| 11 | Evidence that Golgistructure depends on a p115 activity that is independent of thevesicle tether components giantin and GM130显示文摘 | Manojkumar A Puthenveedu Linstedt AD | 2001 | J Cell Biol2001，155，2： | 1 |
| 12 | Failure analysis and remaining life assessment of service exposed primary reformer heater tubes显示文摘 | Jaganathan Swaminathan Krishna Guguloth Manojkumar Gunjan Prabirkumar Roy Rabindranath Ghosh | 2007 | Engineering Failure Analysis2007，，4： | 1 |
| 13 | A randomized, placebocontrolled, double-blind, crossover study to evaluate the efficacy of oval sildenafil therapy in severe pulmonary artery hypertension 显示文摘 | Tarvinder P Sing h Manojkumar Rohit | 2006 | Ameriecan Heart Journal2006，151，4： | 1 |
| 14 | Strength deformation behaviour of circular concrete filled steel tubes subjected to pure bending显示文摘 | Manojkumar V. Chitawadagi Mattur C. Narasimhan | 2009 | Journal of Constructional Steel Research2009，，8： | 1 |
| 15 | Strength deformation behaviour of circular concrete filled steel tubes subjected to pure bending显示文摘 | Manojkumar V. Chitawadagi Mattur C. Narasimhan | 2009 | Journal of Constructional Steel Research2009，，8： | 1 |
| 16 | A rare case of malig- nant fibrous histiocytoma/pleomorphic undifferentiated sarcoma of the kidney显示文摘 | Pathrose G John N T Manojkumar R | 2015 | J Clin Diagn Res2015，9，1： | 1 |
| 17 | Failure analysis and remaining life assessment of service ex posed primary reformer heater tubes显示文摘 | JAGANATHAN S KRISHNA G MANOJKUMAR G etal | 2008 | Engineering Failure Analysis2008，15，4： | 1 |
| 18 | QT interval dispersion in North Indian children with Kawasaki disease without overt coronary artery abnormalities 显示文摘 | Ghelani SJ Singh S Manojkumar R | 2009 | Reumatol lnt2009，30，2： | 1 |
| 19 | Evaluation of protein expression pattern of stanniocalcin 2,insulin-like growth factor-binding protein 7, inhibin beta A and four and ahalf LIM domains 1 in esophageal squamous cell carcinoma显示文摘 | ManojKumar Kashyap HarshA. Pawar Shivakumar Keerthikumar Jyoti Sharma Renu Goel Riaz Mahmood M. Vijaya Kumar K.V. Veerendra Kumar Akhilesh Pandey RekhaV. Kumar T.S.Keshava Prasad H.C. Harsha | 2013 | Cancer Biomarkers2013，，1： | 1 |
| 20 | Synthesis of coumarin heterocyclic derivatives with antioxidant activity and in vitro cytotoxic activity against tumour cells显示文摘 | Manojkumar P Ravi T K Subbuchettiar G | 2009 | Acta Pharm2009，59，2： | 1 |
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