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| 1 | Relationship between T-lymphocyte cytokine levels and sero-response to hepatitis B vaccines显示文摘AIM: To investigate the cellular defects by analyzing the (Th1/Th2) cytokine levels in vaccine responders and non-responders. METHODS: Peripheral blood mononuclear cell (PBMC) from responders and non-responders were stimulated with or with out recombinant HBsAg or PHA. Broad spectrum of cytokines viz (Th1) IFN-γ, IL-2, TNF-α, IL-12 and (Th2) IL-10, IL-4 were measured after in vitro stimulation with recombinant HBsAg and were compared with respective antibody titers. RESULTS: A significant decrease (P = 0.001) in Th1 and Th2 cytokines namely, IL-2, INF-γ, TNF-α and IL-10in non-responders was observed. The level of IL-4 was not significant between the three groups. Furthermore, despite a strong Th1 and Th2 cytokine response, the level of IL-12 was elevated in high-responders compared to other groups (P = 0.001) and demonstrated a positive correlation with anti-HBs titers and Th1 cytokine response. CONCLUSION: Our findings suggest that unrespon-siveness to recombinant hepatitis B vaccines (rHB) is multifactorial, including specific failure of antigen presentation or the lack of both T helper Th1 and Th2 response. | Vijayakumar Velu Shanmugam Saravanan Subhadra Nandakumar Esaki Muthu Shankar Appasamy Vengatesan Suresh Sakharam Jadhav Prasad Suryakant Kulkarni Sadras Panchatcharam Thyagarajan | 2008 | World Journal of Gastroenterology2008，14，22： | 22 |
| 2 | Management of complex and redo cases of pelvic fracture urethral injuries显示文摘Objectives:Pelvic fracture urethral injuries(PFUI)result from traumatic disruption of the urethra.A significant proportion of cases are complex rendering their management challenging.We described management strategies for eight different complex PFUI scenarios.Methods:Our centre is a tertiary referral centre for complex PFUI cases.We maintain a prospective database(1995e2016),which we retrospectively analysed.All patients with PFUI managed at our institute were included.Results:Over two decades 1062 cases of PFUI were managed at our institute(521 primary and 541 redo cases).Most redo cases were referred to us from other centres.Redo cases had up to five prior attempts at urethroplasty.We managed complex cases,which included bulbar ischemia,young boys and girls with PFUI,PFUI with double block,concomitant PFUI and iatrogenic anterior urethral strictures.Bulbar ischemia merits substitution urethroplasty,most commonly,using pedicled preputial tube.PFUI in young girls is usually associated with urethrovaginal fistula.Young boys with PFUI commonly have a long gap necessitating trans-abdominal approach.Our success rate with individualised management is 85.60%in primary cases,79.13%in redo cases and 82.40%in cases of bulbar ischemia.Conclusion:The definition of complex PFUI is ever expanding.The best chance of success is at the first attempt.Anastomotic urethroplasty for PFUI should be performed in experienced hands at high volume centres. | Sanjay BKulkarni Sandesh Surana Devang JDesai Hazem Orabi Subramanian Iyer Jyotsna Kulkarni Ajit Dumawat Pankaj M.Joshi | 2018 | Asian Journal of Urology2018，5，2： | 13 |
| 3 | 盐酸二甲双胍缓释片的溶胀机制与释放规律相关性研究显示文摘以羟丙甲纤维素(HPMC)为骨架材料,采用正交设计优化盐酸二甲双胍缓释片的处方。所得优化处方为:HPMCK100M-药物重量比为0.25∶1,聚乙烯吡咯烷酮(PVP)K90为黏合剂。测定了优化制品在不同pH介质(pH 1.0盐酸、pH 4.5乙酸盐缓冲液和pH 7.4磷酸盐缓冲液)中的溶胀因子和体外释放行为,并用不同模型拟合,继而考察药物释放与缓释片溶胀因子间的相关性。研究表明,所得制品的溶胀与体外释药况均符合Korsmeyer-Peppas模型;药物释放机制为Fick扩散。释药行为不依赖于介质pH的改变,且骨架片的溶胀对药物的释放起主导作用。本品中药物释放与骨架溶胀间具有良好的线性关系。 | 朱芳芳 高慧升 KULKARNI Anand HEGDE Deepak 涂家生 | 2013 | 中国医药工业杂志2013，44，4： | 6 |
| 4 | Ron receptor-dependent gene regulation of Kupffer cells during endotoxemia显示文摘BACKGROUND: Ron receptor tyrosine kinase signaling in macrophages, including Kupffer cells and alveolar macrophages,suppresses endotoxin-induced proinflammatory cytokine/chemokine production. Further, we have also identified genes from Ron replete and Ron deplete livers that were differentially expressed during the progression of liver inflammation associated with acute liver failure in mice by microarray analyses.While important genes and signaling pathways have been identified downstream of Ron signaling during progression of inflammation by this approach, the precise role that Ron receptor plays in regulating the transcriptional landscape in macrophages, and particular in isolated Kupffer cells, has still not been investigated.METHODS: Kupffer cells were isolated from wild-type(TK+/+)and Ron tyrosine kinase deficient(TK-/-) mice. Ex vivo, the cells were treated with lipopolysaccharide(LPS) in the presence or absence of the Ron ligand, hepatocyte growth factor-like protein(HGFL). Microarray and qRT-PCR analyses were utilized to identify alterations in gene expression between genotypes.RESULTS: Microarray analyses identified genes expressed differentially in TK+/+ and TK-/- Kupffer cells basally as well as after HGFL and LPS treatment. Interestingly, our studies identified Mefv, a gene that codes for the anti-inflammatory protein pyrin, as an HGFL-stimulated Ron-dependent gene.Moreover, lipocalin 2, a proinflammatory gene, which is induced by LPS, was significantly suppressed by HGFL treatment.Microarray results were validated by qRT-PCR studies on Kupffer cells treated with LPS and HGFL.CONCLUSION: The studies herein suggest a novel mechanism whereby HGFL-induced Ron receptor activation promotes the expression of anti-inflammatory genes while inhibiting genes involved in inflammation with a net effect of diminished inflammation in macrophages. | Rishikesh M Kulkarni William D Stuart Susan E Waltz | 2014 | Hepatobiliary & Pancreatic Diseases International2014，13，3： | 6 |
| 5 | Evanescent field absorption sensor for detection of copper (Ⅱ) in water using multimode optical fiber显示文摘The evanescent field absorption sensor for the detection of trace amount of copper(II) in water is experimentally analyzed with the etched step index multimode plastic clad silica(PCS) fiber.The designed sensor is sensitive to copper(II) content in water with a range up to 250 ppm. | U. S. Raikar V. K. Kulkarni A. S. Lalasangi I. I. Pattanashetti J.F. Akki | 2009 | Optoelectronics Letters2009，5，3： | 6 |
| 6 | 山嵛酸甘油酯在药剂学中的应用显示文摘山嵛酸甘油酯是一类多用途的长链脂肪酸甘油酯,具有优良的生物相容性,广泛应用于食品、药品和化妆品领域。本文以Gattefosse的产品Compritol 888 ATO为例,从口服给药、注射给药和局部给药三个方面综述山嵛酸甘油酯在药剂学中的应用和研究进展。 | 张伟 贺小玲 杨怡静 Anand Kulkarni Deepak Anant Hegde 涂家生 | 2012 | 药学与临床研究2012，20，6： | 5 |
| 7 | Vascular Anomalies Encountered During Pancreatoduodenectomy: Do They Influence Outcomes?显示文摘 | Parul J. Shukla MS FRCS Savio G. Barreto MS Aniruddha Kulkarni MD Ganesh Nagarajan MS Abe Fingerhut MD FACS FRCS | 2010 | Annals of Surgical Oncology2010，，1： | 5 |
| 8 | Low-velocity simultaneous bilateral femoral neck fracture following long-term antiepileptic therapy:A case report显示文摘BACKGROUND Simultaneous bilateral femoral neck fractures are relatively rare injuries.They are usually associated with underlying metabolic bone disorders or systemic diseases.Long-term use of narcotics and bisphosphonates can also result in similar fracture patterns;however,association of this fracture type with longterm use of antiepileptic drugs is not very common.Only one such case has been reported in the literature.This article describes the second.CASE REPORT We report a case of simultaneous displaced bilateral femoral neck fractures in a 50-year-old epileptic patient,who had taken phenytoin for the past 3 years.The fractures were a result of low-velocity injury following a fall from the bed.The fractures were managed with a bilateral hemi-replacement arthroplasty.Oral bisphosphonates were given to improve the bone quality in the post-operative period.The patient had a good post-operative outcome,that was sustained throughout the entire follow-up period of 1 year.CONCLUSION Antiepileptic drugs should be supplemented with bisphosphonates and vitamin D to improve bone quality and prevent fractures in epileptic patients. | Mohammed Sadiq Vikrant Kulkarni Syed Azher Hussain Mohammed Ismail Mayur Nayak | 2019 | World Journal of Orthopedics2019，10，10： | 5 |
| 9 | Antimicrobial metabolites from marine microorganisms显示文摘Marine ecological niches have recently been described as 'particularly promising' sources for search of new antimicrobials to combat antibiotic-resistant strains of pathogenic microorganisms. Marine organisms are excellent sources for many industrial products, but they are partly explored. Over 30 000 compounds have been isolated from marine sources. Bacteria, fungi, and cyanobacteria obtained from various marine sources secret several industrially useful bioactive compounds, possessing antibacterial, antifungal, and antimycobacterial activities. Sustainable cultivation methods for promising marine organisms and biotechnological processes for selected compounds can be developed, along with the establishment of biosensors for monitoring the target compounds. The semisynthetic modifications of marine-based bioactive compounds produce their new derivatives, structural analogs and mimetics that could serve as novel lead compounds against resistant pathogens. The present review focuses on promising antimicrobial compounds isolated from marine microbes from 1991-2013. | Prasanna Habbu Vijayanand Warad Rajesh Shastri Smita Madagundi Venkatrao H. kulkarni | 2016 | Chinese Journal of Natural Medicines2016，14，2： | 5 |
| 10 | Comparison of three different recombinant hepatitis B vaccines:GeneVac-B,Engerix B and Shanvac B in high risk infants born to HBsAg positive mothers in India显示文摘AIM: To evaluate a low cost Indian recombinant hepatitis B vaccine GeneVac-B for its immunogenicity and safety in comparison to Engerix B and Shanvac B vaccine in high risk newborn infants born to hepatitis B surface antigen (HBsAg) positive mothers. METHODS: A total of 158 infants were enrolled in the study. Fifty eight infants were enrolled in the GeneVac-B group while 50 each were included for Engerix B and Shanvac B groups. A three-dose regimen of vaccination; at birth (within 24 h of birth), 1st mo and 6 mo. were adopted with 10 μg dosage administered uniformly in all the three groups. Clinical and immunological parameters were assessed for safety and immunogenicity of the vaccines, in all the enrolled infants. RESULTS: Successful follow up until seven months of age was achieved in 83/ (48/58) for GeneVac-B, 76/ (38/50) and 64/ (32/50) for Engerix B and Shanvac B groups respectively. 100/ seroconversion and seroprotection was achieved in all the three groups of infants. The geometric mean titers of anti-HBs one month after the completion of three dose of vaccination were 90.5, 80.9 and 72.5 mIU/mL in GeneVac-B, Engerix B and Shanvac B vaccine group respectively. Furthermore the level of anti-HBs increases with age ofbabies who were born to HBsAg positive mothers. The GMT values of anti-HBs were 226.7, 193.9 and 173.6 mIU/mL respectively in GeneVac-B, Engerix B and Shanvac B groups one year after the completion of the three doses of vaccine. No systemic reactions were reported in infants during the entire vaccination process of GeneVac-B and the other two vaccines. Clinical safety parameters remained within the normal limits throughout the study period.CONCLUSION: The study concludes that there is no significant difference between the three recombinant hepatitis B vaccines. Administration of these vaccines within 24 h of birth to babies, born to HBsAg positive mothers will reduce the incidence of HBV infection. | Vijayakumar Velu Subhadra Nandakumar Saravanan Shanmugam Suresh Sakharam Jadhav Prasad Suryakant Kulkarni Sadras Panchatcharam Thyagarajan | 2007 | World Journal of Gastroenterology2007，13，22： | 4 |
| 11 | Soil and water conservation measures improve soil carbon sequestration and soil quality under cashews显示文摘Land degradation is becoming a serious problem in the west coast region of India where one of the world's eight biodiversity hotspots,the‘Western Ghats’,is present.Poor land management practices and high rainfall have led to increasing problems associated with land degradation.A long-term(13-year)experiment was done to evaluate the impact of soil and water conservation measures on soil carbon sequestration and soil quality at three different depths under cashew nut cultivation on a 19%slope.Five soil and water conservation measures-continuous contour trenches,staggered contour trenches,halfmoon terraces,semi-elliptical trenches,and graded trenches all with vegetative barriers of Stylosanthes scabra and Vetiveria zizanoides and control were evaluated for their influence on soil properties,carbon sequestration,and soil quality under cashews.The soil and water conservation measures improved significantly the soil organic carbon,soil organic carbon stock,carbon sequestration rate and microbial activity compared to the control condition(without any measures).Among the measures tested,continuous contour trenches with vegetative barriers outperformed the others with respect to soil organic carbon stock,sequestration rate,and microbial activity.The lower metabolic quotient with the measures compared to the control indicated alleviation of environmental stress on microbes.Using principal component analysis and a correlation matrix,a minimum dataset was identified as the soil available nitrogen,bulk density,basal soil respiration,soil pH,acid phosphatase activity,and soil available boron and these were the most important soil properties controlling the soil quality.Four soil quality indices using two summation methods(additive and weighted)and two scoring methods(linear and non-linear)were developed using the minimum dataset.A linear weighted soil quality index was able to statistically differentiate the effect of soil and water conservation measures from that of the control.The highest value of the soil quality index of 0.98 was achieved with continuous contour trenches with a vegetative barrier.The results of the study indicate that soil and water conservation measures for cashews are a potential strategy to improve the soil carbon sequestration and soil quality along with improving crop productivity and reducing the erosion losses. | Gopal Ramdas Mahajan Bappa Das Sandrasekaran Manivannan Begur Lakshminarasimha Manjunath Ram Ratan Verma Sujeet Desai Rahul Mukund Kulkarni Ashish Marotrao Latare Reshma Sale Dayesh Murgaonkar Kiran Puna Patel Shaiesh Morajkar Ashwini Desai Natasha Barnes Heena Mulla | 2021 | International Journal of Sediment Research2021，36，2： | 4 |
| 12 | Cyr61,一个潜在的前列腺癌预后生物标记物显示文摘富半胱氨酸血管生成诱导因子61(Cyr61)是一种细胞外基质蛋白,与生长因子和激素的信号转导相关,且其表达量在几种癌症中会经常发生变化。Cyr61在器官局限性前列腺癌中呈高度表达。此外,Cyr61的表达水平与较低的疾病复发风险相关,且在血清中能够定量测定。综合考虑,上述结果表明Cyr61是一种潜在的,临床上实用的、基于组织和血清的生物标记物,可用于区分致死及非致死性前列腺癌。 | Naoki Terada Prakash Kulkarni Robert H Getzenberg | 2012 | Asian Journal of Andrology2012，14，3： | 4 |
| 13 | Facet selective etching of Au microcrystallites显示文摘High-symmetry crystals exhibit isotropic properties. Inducing anisotropy, e.g., by facet selective etching, is considered implausible in face-centered cubic (FCC) metals, particularly gold, which, in addition to being an FCC, is noble. We report for the first time the facet selective etching of Au microcrystals obtained in the form of cuboctahedra and pentagonal rods from the thermolysis of a gold- organic precursor. The selective etching of {111} and {100} facets was achieved using a capping method in which tetraoctylammonium cations selectively cap the {111} facets while Br- ions protect the {100} facets. The exposed facets are oxidized by O2/C1-, yielding a variety of interesting geometries. The facet selective etching of the Au microcrystallites is governed only by the nature of the facets; the geometry of the microcystallite does not appear to play a significant role. The etched surfaces appear rough, but a closer examination reveals well-defined corrugations that are indexable to high hkl values. Such surfaces exhibit enhanced Raman activity. | Gangaiah Mettela and Giridhar U. Kulkarni | 2015 | Nano Research2015，8，9： | 3 |
| 14 | Prostate-associated gene 4 (PAGE4), an intrinsically disordered cancer/testis antigen, is a novel therapeutic target for prostate cancer显示文摘联系前列腺的基因(PAGE4 ) 4 是是的显著地前列腺特定的癌症 / 睾丸抗原高度,在人的胎儿的前列腺和它的 diseased 的 upregulated 说然而并非在成年的正常的腺。PAGE4 是作为压力反应蛋白质工作压制反应的氧种类以及阻止 DNA 损坏的内在地混乱的蛋白质(IDP ) 。另外, PAGE4 也是加强的一个 transcriptional 管理者由 oncogene c-Jun.c6 月的 transactivation 与 Fos 家庭的成员一起由 heterodimerizing 形成 AP-1 建筑群并且在前列腺的发展和病理起一个重要作用,强调 PAGE4/c-Jun 相互作用的重要性。HIPK1,另外压力反应小径的一个部件,在为它的 transcriptional 活动批评的 T51 的 phosphorylates PAGE4。Phosphorylation 导致 conformational 并且在导致新细胞的功能的 PAGE4 整体的动态切换。最后,生物信息学证据建议 PAGE4 mRNA 能或者被拼接导致与潜伏的功能提到 conformational 整体的一个范围的可能性的多肽的四潜在的 isoforms。一起考虑了,数据建议 PAGE4 可以代表在应力和前列腺癌症(PCa ) 之间的第一个分子的连接。因此, pharmacologically 指向 PAGE4 可以是为与 PCa 对待并且设法病人的一个新奇机会,特别有低风险的疾病的病人。 | Prakash Kulkarni A Keith Dunker Keith Weninge John Orban | 2016 | Asian Journal of Andrology2016，18，5： | 3 |
| 15 | Meeting report:a hard look at the state of enamel research显示文摘The Encouraging Novel Amelogenesis Models and Ex vivo cell Lines(ENAMEL) Development workshop was held on 23 June 2017 at the Bethesda headquarters of the National Institute of Dental and Craniofacial Research(NIDCR). Discussion topics included model organisms, stem cells/cell lines, and tissues/3 D cell culture/organoids. Scientists from a number of disciplines,representing institutions from across the United States, gathered to discuss advances in our understanding of enamel, as well as future directions for the field. | ophir d klein olivier duverger wendy shaw rodrigo s lacruz derk joester janet moradian-oldak megan k pugach j timothy wright sarah e millar ashok b kulkarni john d bartlett thomas gh diekwisch pamela den besten james p simmer | 2017 | International Journal of Oral Science2017，9，4： | 3 |
| 16 | Evaluation of Diffusion-weighted MR Imaging for Detection of Bowel Inflammation in Patients with Crohn’s Disease显示文摘 | Aytekin Oto Fang Zhu Kirti Kulkarni Gregory S. Karczmar Jerrold R. Turner David Rubin | 2009 | Academic Radiology2009，，5： | 3 |
| 17 | Ron receptor-dependent gene regulation in a mouse model of endotoxin-induced acute liver failure显示文摘BACKGROUND:Prior experimentation has shown that loss of the tyrosine kinase(TK) signaling domain of the Ron receptor leads to marked hepatocyte protection in a model of lipopolysaccharideinduced acute liver failure(ALF) in D-galactosamine(GalN)sensitized mice.The aim of this study was to identify the role of Ron in the regulation of hepatic gene expression.METHODS:Microarray analyses were performed on liver RNA isolated sequentially from wild-type(WT) and TK-/mice during the progression of ALF.Gene array data were validated using Western and immunohistochemistry analyses as well as with ex vivo culture systems.RESULTS:At baseline,101 genes were differentially expressed between WT and TK-/-livers,which regulate processes involved in hypoxia,proliferation,apoptosis and metabolism.One hour after ALF induction,WT livers exhibited increased cytokine expression compared to TK-/-livers,and after 4 hours,an induction of suppressor of cytokine signaling(SOCS) genes as well as JAK-STAT pathway activation were prominent in TK-/livers compared to controls.CONCLUSION:Our studies suggest a novel hepato-protective mechanism in Ron TK-/-mice wherein increased and sustained SOCS production and JAK-STAT activation in the hepatocyte may inhibit the destructive proinflammatory milieu and promote survival factors which blunt hepatic death and the ensuing development of ALF. | Rishikesh M Kulkarni Louis W Kutcher William D Stuart Daniel J Carson Mike A Leonis Susan E Waltz | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，4： | 3 |
| 18 | Effect of process parameters on electromagnetic impact welding of aluminum sheets显示文摘 | S.D. Kore P.P. Date S.V. Kulkarni | 2006 | International Journal of Impact Engineering2006，，8： | 3 |
| 19 | Self-assembled vertically aligned gold nanorod super-lattices for ultra-high sensitive detection of molecules显示文摘我们显示出超点阵能作为探针或底层服务为的那自我装配的垂直地排列的金 nanorod (VA-GNRs ) 超离频各种各样的分子的敏感察觉。d葡萄糖和2,4,6强烈炸药( TNT )由于他们的很低的拉曼剖面图被选择了为模型系统( 5.6 佩?????汣獡????汰獵瀭畬???㈠?猯'T￣慮潮畴敢??????????????????????吗???????????????????￡???霔?? | Amey Apte Prashant Bhaskar Raja Das Smita Chaturvedi PankajPoddar Sulabha Kulkarni | 2015 | Nano Research2015，8，3： | 3 |
| 20 | Monitoring Response to Antiangiogenic Treatment and Predicting Outcomes in Advanced Hepatocellular Carcinoma Using Image Biomarkers, CT Perfusion, Tumor Density, and Tumor Size (RECIST)显示文摘 | Tao Jiang Avinash Kambadakone Naveen M. Kulkarni Andrew X. Zhu Dushyant V. Sahani | 2012 | Investigative Radiology2012，，1： | 3 |
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